Rodent Surgery Course

Why????• Laws and regulations dictate appropriate

procedures and care

Laws and Regulations

You have a contract with the AIC Surgical variables can affect

animal welfare and research

You Have a Responsibility to the Animal

Success: Requires careful

planning

Challenges…. Same person is surgeon, anesthetist, scrub

nurse Experimental design requires repetitive

surgeries Small body size of rodents mandates use of

microsurgery or ophthalmic instruments Immunodeficient rodents

Major Survival Surgery “Any surgical intervention that penetrates

and exposes a body cavity or any procedure which produces permanent impairment of physical or physiologic functions”

Minor Survival Surgery Surgery that “does not expose a body

cavity and causes little or no physical impairment”

Non rodents: Dedicated surgical facility

Rodents: Surgical facility not required

Rodent Surgery Area Room or part of a room that is easily

sanitized and not used for other activities when rodent surgery is in progress

Rodent Surgery Area Subdivided into:

Place for cages of rodents awaiting surgery Prep area Surgery area Recovery area

Anesthesia

Inhalation Injectable

Instruments Quality Appropriate size for

procedure Correct utilization

Surgical Pack Order Sterilization

indicators

Wound Closure

Surgical Needles

Surgical Adhesive

Preoperative Management Animal health status Period of stabilization Proper handling and restraint Stress-reduction Food and water NOT withheld AVOID antimicrobial agents

Perioperative Management Prepare surgery area

organize disinfect

Sterilization of Instruments Autoclave

Indications of Sterilization

Glass Bead Sterilizer Provides rapid

sterilization

Chemical Time lengthy Kill spores?????

Patient Prep

Prevent Hypothermia

                               

Surgeon Prep

Operative Technique Follow aseptic

surgery procedures

Utilize good surgical technique

Tunnel vascular catheters

Operative Care Monitoring the

patient

Post-operative Care Analgesia Hydration Nutrition Wound care Suture removal

Maintaining Records Procedure and date Post op care Suture removal

Monitoring Outcomes

PI’s for donating extra animals for this lab!!