RNA$Extrac+on:$Trizol -...

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RNA Extrac+on: Trizol

Transcript of RNA$Extrac+on:$Trizol -...

Page 1: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA  Extrac+on:  Trizol

Page 2: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA  Extrac+on:  Trizol

Page 3: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA  extrac+on  from  cells,  +ssues,  organs

Page 4: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Mammalian  +ssues  (DEPC  treated)

Page 5: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA  Extrac+on

Page 6: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA  Analysis

OD260/OD280:  1.9-­‐2.1                  GOOD  <1.8                            Protein  contamina+on  >2.2                            RNA  degreda+on    OD260/OD230:  <2.0                            Trizol  contamina+on

Page 7: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA  Electrophoresis

Page 8: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

mRNA  prepara+on

Page 9: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA
Page 10: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Reverse  Transcrip+on  PCR

Page 11: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Hybridiza+on

Page 12: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA
Page 13: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Northern  Blot

Page 14: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

Northern  Blot

Page 15: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA  in  situ  hybridiza+on

Page 16: RNA$Extrac+on:$Trizol - person.zju.edu.cnperson.zju.edu.cn/attachments/2012-10/07-1350281434-653711.pdf · 23S rRNA 16S rRNA • 28 s 18 s SIM Agarose gel electrophoresis for RNA

RNA  in  situ  hybridiza+on


Targeting of RNA into mitochondria: mechanistic and ... · nDNA >1000 proteins, several ncRNAs (tRNAs, MRP RNA, Rnase P RNA, 5S rRNA) Genetic double-dependence . Import of RNA into

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Triphosphate residues at the 5' ends of rRNA precursor and 5S RNA from Dictyostelium discoideum

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Molecular phylogenetic analysis using ribosomal RNA (rRNA)

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Comprehensive characterization of four different ...en.terapiekomorkowe.pl/wp-content/uploads/2016/08/Comprehensive... · Total cellular RNA was extracted using TRIzol reagent (Invitrogen

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RNase MRP and the RNA Processing Cascade in the Eukaryotic ... · RNA-processing cascade. This cascade is centred around the processing of three types of RNA, mRNA, tRNA and rRNA

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RNA Secondary Structure Prediction. 16s rRNA RNA Secondary Structure Hairpin loop Junction (Multiloop)Bulge Single- Stranded Interior Loop Stem Image–

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Nuclear Pore Flow Rate of Ribosomal RNA and Chain Growth ... · PDF fileof cytoplasmic rRNA ... sponding stationary RNA distribution: over 90% of the egg RNA consists of 18 S ... certain

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RNA processing #1 Making ends of RNA. Types of RNA processing A) Cutting and trimming to generate ends: –rRNA, tRNA and mRNA B) Covalent modification:

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A novel stress response pathway regulates rRNA biogenesis · 16.08.2020  · 38 RNA synthesis is rRNA. Maturation of the 47S rRNA requires multiple endonucleolytic and exonucleolytic

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TRIzol products—validated for high-quality RNA · TRIzol PLUS RNA Purification Kit 1 10 100 1,000 1 10 100 1,000 Starting material (mg) Yield of total RNA (µg) Supplier Q TRIzol

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RNA Metabolism - Creightonchemistry.creighton.edu/~jksoukup/lec27.pdfRNA Metabolism Transcription - process by which DNA (genetic info) gets made into RNA (mRNA, rRNA, or tRNA) by

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Ribosomal RNA Transcription, Processing and Modification · Ribosomal RNA. Transcription, Processing and Modification. rRNA constitute 80% of the RNA in rapidly dividing cell! A growing

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Other RNA Processing Events Chapter 16. Ribosomal RNA Processing rRNA genes of both eukaryotes and bacteria are transcribed as larger precursors must.

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Section O – RNA processing and RNPs. O1 rRNA processing and ribosomes Types of RNA processing, rRNA processing in prokaryote, rRNA processing in eukaryotes,

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genesdev.cshlp.orggenesdev.cshlp.org/.../SuppText_TablesS1-S5.docx · Web viewCrypts were immediately centrifuged at 4 C and re-suspended in 800 µl Trizol for RNA Isolation, and

genesdev.cshlp.orggenesdev.cshlp.org/.../SuppText_TablesS1-S5.docx · Web viewCrypts were immediately centrifuged at 4 C and re-suspended in 800 µl Trizol for RNA Isolation, and

Quick-DNA Miniprep Plus - Zymo Research...other epigenetic tools. For RNA isolation from TRIzol®, the Direct-zol RNA Plus Kits (R2070, R2072, R2071, R2073) offer total RNA purification

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Biosafety - جامعة نزوى · Hplc High performance liquid chromatography ... ppt Phosphinothricin Qtl quantitative trait loci rna Ribonucleic acid rrna Ribosomal RNA rt-pcr

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