Ribosome footprinting Not all RNAs encode proteins.
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Transcript of Ribosome footprinting Not all RNAs encode proteins.
Note 3’UTRs Ribosome + mRNA Also note that coding regions are highly
conserved by a conservation score CSF Codon substitution frequency
Yet, lincRNAs look more like translated regions and 5’UTRs based on
translational efficiency (TE = ribosome/mRNA)
Why high TE for lincRNAs?
• Reasons the authors give:– Non-ribosomal contamination– Seductive hypothesis in the discussion:• Translational machinery is always engaged with
transcripts in the cytosol of the cell.• “Pervasive” engagement of the ribosome with
transcripts.– This can be co-opted by evolution to create new protein-
coding genes.