Rhodopsin:Effect of replacement of amino acids in the predicted folding core of Rhodopsin Growth hormone receptor (GHR):Expression of Cytoplasmic domain of GHR4 in E.coli,

Epidermal Growth Factor (EGF):Secretory and extracelluar production of human EGF

Ciliary Neurotrophic Factor Alpha Receptor (CNTFR):Expression of Cytokine binding domain (CBD) of CNTFR

Rhodopsin

ResidueNameS22CR1F24CR2E25CR3A26CR4A27CR5N111CR6L112C/F116CdR7L112CR7F116CR8A166CR9A168CR10A169CR11

Bovine Rhodopsin

GAATTCATGAACGGTACCGAAGGCCCAAACTTCTACGTTCCTTTCTCCAACAAGACGGGCGTGGTGCGCAGCCCGTTCGAGGCTCCGCAGTACTACCTGGCGGAGCCCTGGCAGTTCTCCATGCTGGCCGCCTACATGTTCCTGCTGATCATGCTTGGCTTCCCGATCAACTTCCTCACGCTGTACGTCACAGTCCAGCACAAGAAGCTTCGCACACCGCTCAACTACATCCTGCTCAACCTGGCCGTGGCAGATCTCTTCATGGTCTTCGGTGGCTTCACCACCACCCTCTACACCTCTCTCCATGGGTACTTCGTCTTTGGGCCGACGGGCTGCAACCTCGAGGGCTTCTTTGCCACCCTGGGCGGTGAAATTGCACTGTGGTCTCTGGTAGTACTGGCGATCGAGCGGTACGTGGTGGTGTGCAAGCCCATGAGCAACTTCCGCTTCGGTGAGAACCACGCCATCATGGGCGTCGCCTTCACCTGGGTCATGGCTCTGGCCTGTGCGGCCCCGCCGCTCGTCGGCTGGTCTAGATACATCCCGGAGGGCATGCAGTGCTCGTGCGGGATCGATTACTACACGCCGCACGAGGAGACCAACAATGAGTCGTTCGTCATCTACATGTTCGTGGTCCACTTCATCATCCCGCTGATTGTCATCTTCTTCTGCTATGGCCAGCTGGTGTTCACCGTCAAGGAGGCTGCAGCCCAGCAGCAGGAGAGCGCCACCACTCAGAAGGCCGAGAAGGAGGTCACGCGTATGGTTATCATCATGGTCATCGCTTTCCTAATCTGCTGGCTGCCCTATGCTGGTGTGGCGTTCTACATCTTCACCCATCAGGGCTCTGACTTTGGGCCCATCTTCATGACCATCCCGGCTTTCTTTGCCAAGACGTCTGCCGTCTACAACCCGGTCATCTACATCATGATGAACAAGCAGTTCCGGAACTGCATGGTCACCACTCTCTGCTGTGGCAAGAACCCGCTGGGTGACGACGAGGCGTCGACCACCGTCTCCAAGACAGAGACCAGCCAAGTGGCGCCTGCCTAAGCGGCCGC

MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA

(Underlined nucleotide has been modified, opsin gene in PMT4 is different to the original sequence as shown above)

Relative ratio A280/A500 of rhodopsin wide Type (WT) and 11 mutantsMutants F24C, A26C, L112C/F116C, F116C and A168C showed relative ratio between 500nm and 280nm were greater than 8Mutants S22C, E25C, A27C, N111C, A166C and A169C showed less than 5[Further experiments are still need to perform to further verify before the classified the mutants]

Chart18

2.30.33

4.170.61

19.112.36

3.840.27

9.360.96

4.150.69

2.540.21

20.195.24

14.143.75

3.10.25

7.740.84

2.530.12

Relative Ratio of A280/A500

Sheet1

WTR1R2R3R4R5R6R7R8R9R10R11

WTS22CF24CE25CA26CA27CN111CL112C/F116CF116CA166CA168CA169C

2.34.1719.113.849.364.152.5420.1914.143.17.742.53

0.330.612.360.270.960.690.215.243.750.250.840.12

Sheet1

00.33

00.61

02.36

00.27

00.96

00.69

00.21

05.24

03.75

00.25

00.84

00.12

Relative Ratio of A280/A500

Sheet2

Sheet3

Elution profile of Rhodopsin mutant A169C first with NaPi buffer and then followed buffer with 150mM NaCl

Chart3

0.0042170.058021

0.0239170.087996

0.046720.107994

0.0266410.066425

0.0221810.05174

0.0183270.041144

0.0234360.056261

0.0542180.14752

0.0459720.110377

0.034230.080516

0.0294290.068123

0.0223780.05962

Absorbance 500 nm

Absorbance 280 nm

Fraction

Absorbance

Elution Profile of Rhodopsin Mutant A169C from 1D4 Sepharose Column

WT 1

6500.0018480.000083-0.000092-0.00211-0.001176-0.002988-0.0030930.0073640.0240430.0188040.0131450.0097770.0055250.005014

6490.0019130.000074-0.000078-0.002129-0.001258-0.003011-0.0032310.0076179310.0248720690.01945241380.01359827590.01011413790.00571551720.0051868966

6480.0019130.000037-0.000032-0.002065-0.001213-0.003011-0.003386totalper plate

6470.0018390.000051-0.000092-0.002102-0.001291-0.003053-0.0032270.08655724140.0173114483

6460.0019690.000133-0.00006-0.001951-0.001286-0.00298-0.003226

6450.0019830.000133-0.000041-0.002033-0.001204-0.003113-0.003209280-6500.037510.0489920.0361950.0277740.0232450.014280.014075

6440.002020.000120.000009-0.002061-0.001195-0.003063-0.003264500-6500.0073640.0240430.0188040.0131450.0097770.0055250.005014

6430.00190.000032-0.000097-0.002085-0.001287-0.003105-0.003292280/5005.09369907662.03768248551.92485641352.11289463672.37751866632.58461538462.807140008

6420.0019470.0000460.000028-0.002057-0.001236-0.003151-0.003324stdev 71.0986792164

6410.0019560.000041-0.000074-0.002108-0.001365-0.003192-0.003485stdev 60.343052336

6400.0019660.000083-0.000143-0.002044-0.001379-0.003316-0.003517error 70.415261711

6390.0020120.000055-0.00012-0.002072-0.001393-0.00328-0.003453error 60.1400505297

6380.0019340.000028-0.00017-0.002141-0.001407-0.00328-0.003545Ave of 72.7054866673

6370.0019620.000037-0.00023-0.002072-0.001481-0.003386-0.003642Ave of 62.3074512658

6360.001935-0.000028-0.000184-0.002132-0.001476-0.00346-0.00367

6350.001972-0.000129-0.000253-0.002188-0.001454-0.003461-0.003712

6340.002019-0.000005-0.00017-0.002183-0.001514-0.003571-0.003657

6330.001968-0.000129-0.000198-0.002138-0.001629-0.003649-0.003622

6320.001918-0.000124-0.000267-0.002212-0.001647-0.003673-0.00381

6310.002024-0.000055-0.000217-0.002166-0.001542-0.003673-0.00375

6300.002122-0.000023-0.000152-0.00213-0.001569-0.003751-0.003746

6290.0021170.000074-0.000074-0.00207-0.001634-0.003723-0.003696

6280.002034-0.000023-0.000157-0.002172-0.00163-0.003789-0.003839

6270.002057-0.000065-0.000157-0.00208-0.001607-0.003876-0.003972

6260.002160.000083-0.000157-0.002048-0.001634-0.003821-0.003858

6250.00216-0.000023-0.000115-0.002158-0.001644-0.003831-0.003927

6240.002132-0.000005-0.000175-0.002122-0.001658-0.00395-0.003918

6230.002184-0.000037-0.000157-0.002136-0.001612-0.003987-0.004001

6220.0022390.000074-0.000185-0.002118-0.00164-0.003882-0.003983

6210.0021790.000088-0.000208-0.002044-0.001663-0.003933-0.004097

6200.002272-0.000023-0.000203-0.002086-0.001668-0.003938-0.004025

6190.0022310.000014-0.000198-0.002031-0.001755-0.00397-0.004107

6180.0022730.000005-0.000226-0.002082-0.001741-0.004043-0.004085

6170.0022310.000023-0.000152-0.002091-0.001696-0.004062-0.004163

6160.0022680.000014-0.00012-0.002169-0.00176-0.004067-0.004223

6150.0023330-0.000166-0.002096-0.001719-0.004127-0.004085

6140.0022550.000111-0.000194-0.002022-0.001825-0.004159-0.004205

6130.0024540.000166-0.000005-0.001967-0.001737-0.004026-0.004017

6120.0024170.000134-0.000018-0.00199-0.001742-0.003999-0.004136

6110.002390.000092-0.000102-0.001995-0.001844-0.004211-0.004192

6100.0024040.000203-0.000051-0.001931-0.00171-0.004284-0.004129

6090.0023530.000125-0.000028-0.002001-0.001697-0.00417-0.004221

6080.0023950.0002080.000097-0.001936-0.001656-0.00423-0.004052

6070.002480.000217-0.000083-0.001955-0.001799-0.004323-0.004062

6060.0024390.0001940.000046-0.001974-0.001712-0.004173-0.00421

6050.0025040.0002590.000106-0.002034-0.00168-0.004183-0.00416

6040.0023050.0003290.000051-0.001851-0.001676-0.004179-0.004083

6030.0023790.0005180.000319-0.001703-0.001505-0.004065-0.004065

6020.0022790.0003660.000185-0.001806-0.001622-0.00421-0.004004

6010.0025310.0004170.000213-0.001774-0.001461-0.004005-0.00406

6000.0026810.0005650.000352-0.001687-0.001443-0.004089-0.00402

5990.0027520.0005750.000413-0.001674-0.001393-0.004041-0.003843

5980.0028650.0007330.000566-0.001508-0.001338-0.003922-0.003899

5970.0024980.0007010.000413-0.001565-0.001413-0.004008-0.00393

5960.0023040.000720.000465-0.001553-0.001405-0.004034-0.003906

5950.0024690.0008930.000632-0.001368-0.001211-0.003995-0.003875

5940.0025120.0009860.000623-0.001443-0.001174-0.003868-0.003772

5930.0025150.0010010.000694-0.001352-0.001208-0.004024-0.003632

5920.0025260.0010250.000676-0.001265-0.001223-0.003949-0.003741

5910.0024810.0010260.000863-0.001372-0.001159-0.003952-0.003739

5900.0022920.0010090.000775-0.001304-0.001244-0.004086-0.003845

5890.0022230.0011220.000822-0.001282-0.001226-0.004061-0.00388

5880.0020650.0012960.00087-0.001278-0.001241-0.003994-0.003777

5870.002010.001470.001015-0.001125-0.001032-0.003969-0.003788

5860.0019790.0014860.001082-0.001159-0.001117-0.003856-0.003722

5850.0018060.0015660.001242-0.001047-0.001089-0.004026-0.003882

5840.0015910.0016520.001196-0.001156-0.001151-0.003979-0.003993

5830.0016060.0016910.001432-0.001063-0.00104-0.004018-0.003865

5820.0018240.0019890.001433-0.000928-0.001031-0.003922-0.003927

5810.0019290.0018810.001533-0.001031-0.000881-0.004027-0.003962

5800.0017930.0021270.001717-0.000849-0.000872-0.003935-0.003856

5790.0019490.002270.001671-0.000831-0.000859-0.003942-0.004021

5780.0019120.0023840.001865-0.000812-0.000709-0.003901-0.003748

5770.0016480.0025740.002002-0.0007-0.000765-0.00387-0.003739

5760.0019360.0027210.00222-0.000573-0.000592-0.003823-0.003777

5750.0021020.0029250.002244-0.000503-0.000644-0.003853-0.00382

5740.0024610.0031380.002608-0.000348-0.000371-0.003718-0.003568

5730.0027130.00330.002741-0.000343-0.000442-0.00376-0.003648

5720.0031010.0035650.002722-0.000089-0.000348-0.003685-0.003676

5710.0032810.0036410.003091-0.000038-0.000244-0.003648-0.003476

5700.003210.0038740.0030630.000052-0.000212-0.003523-0.003541

5690.0033140.0040680.0033140.000273-0.00008-0.003518-0.003532

5680.003390.0042350.0034140.000263-0.000099-0.003537-0.003415

5670.0034140.0045770.0036270.0003390.000075-0.003485-0.003336

5660.0033670.0046680.0037790.0003860.000094-0.003528-0.003458

5650.0035270.0050140.0039310.0005980.000263-0.003438-0.003438

5640.003670.0051340.0041020.0007010.000311-0.003346-0.003374

5630.0036650.0054470.0045190.000880.000598-0.003251-0.003237

5620.0037070.0058370.0046710.0010120.000645-0.002994-0.003153

5610.0037260.0060230.0048610.0010830.000758-0.003144-0.003139

5600.0037070.0062810.0050610.0010730.000819-0.003135-0.002948

5590.0039430.0067790.0053730.0015780.001054-0.003035-0.002998

5580.0040830.0070710.0057390.0016970.001145-0.002682-0.002813

5570.0041460.0075470.0061220.0020470.001447-0.002641-0.002632

5560.0042420.0077960.006280.0021460.001504-0.002637-0.00266

5550.0042130.0080410.0065620.0022930.001674-0.00251-0.00252

5540.004470.0084860.0068150.0025010.001759-0.00237-0.002468

5530.0044740.0088160.0070770.0026760.002108-0.002178-0.00222

5520.0046160.0093340.0074350.0029030.002113-0.002098-0.002163

5510.0046870.0096080.0078460.0030830.002335-0.001999-0.002201

5500.004830.0100310.007980.0032440.002392-0.00192-0.002084

5490.004910.0104010.0082910.0034520.002675-0.001779-0.001952

5480.0050620.0106660.008660.003680.002732-0.001784-0.001877

5470.0050570.0110520.00890.0038220.002808-0.001624-0.001863

5460.0051420.0116090.0092770.0041340.003176-0.001408-0.001629

5450.0051750.0119130.0095560.0043480.003176-0.001277-0.001451

5440.0052980.0123420.0098340.0045750.003484-0.001239-0.001371

5430.0053220.0127670.0102370.0048080.003687-0.001033-0.001361

5420.0054780.0131730.0104340.0049930.003777-0.000939-0.001155

5410.0055070.0136140.0108820.0053070.004067-0.00078-0.001094

5400.0056020.0139590.0111380.0054930.00429-0.000653-0.00085

5390.0058220.0144040.0114970.0057030.004367-0.00054-0.000817

5380.0056810.0147130.0116730.0058520.00452-0.000437-0.000733

5370.005890.0152390.0121420.0061530.004734-0.000273-0.000611

5360.0061760.0156660.0123730.0063760.004985-0.000094-0.00039

5350.0061580.0160390.0126990.0065970.0051290.000005-0.000296

5340.0062330.0164420.0131090.0070160.0054040.000395-0.000183

5330.0062730.0169050.0134030.0069990.0054670.000306-0.000169

5320.0063540.01720.0136810.0072380.0056820.000386-0.000061

5310.0063690.0175820.0138950.0074350.0057580.0005040.000151

5300.0065420.0179770.0142520.0076080.0059360.0006260.000151

5290.0066040.0182530.0145250.0077710.0060980.0005930.000249

5280.0068080.0188730.0147940.0080670.0063160.0009090.000329

5270.0069440.0190690.0149440.0081980.0063660.0009850.000311

5260.0068910.0193940.0151640.0083610.0064660.0009850.000504

5250.007050.0196310.0153840.0087220.0066770.0011310.000541

5240.0071550.0201190.0156820.008650.0067630.001220.000584

5230.0072810.0203750.0159140.0087050.0069120.001240.00073

5220.0073580.0206370.0161740.0089830.0071040.0012870.000674

5210.0073490.0208720.0163470.0091820.0071910.0014240.000782

5200.0075220.0212190.0165090.0092980.0072820.0015140.000933

5190.0075550.0214760.0167820.0093940.007320.0016040.00098

5180.007860.0217980.0171170.0096760.0076110.001840.001202

5170.0081350.0220240.0172120.0098550.0076790.0018260.001339

5160.0083930.0222790.0174110.0099940.0079760.0020330.001315

5150.0083560.0224630.0174550.0100060.0078530.0019770.001438

5140.008510.0226210.0176350.0102080.0078720.0020290.001415

5130.0084680.0227640.0176580.0101080.0080410.0019020.001254

5120.0086070.0230160.018030.0103390.008060.0021050.001377

5110.0085640.0230610.0179080.0103690.008070.0020530.001486

5100.0085590.0232110.0181050.0103550.0082040.0020770.001552

5090.0086190.0233450.0180050.0105010.0080910.0021390.001571

5080.0087710.0235050.0182040.0105570.0082910.0022470.001547

5070.0087570.0236650.0183320.0107070.008430.0023180.001651

5060.0087830.0236170.0184060.0106720.0083370.002210.001406

5050.0088790.0238050.0184550.0107580.0084420.0023050.001666

5040.008830.0237050.0184080.0106080.0082150.0021820.001548

5030.0089870.0240080.0184430.0108230.0085350.0024280.001633

5020.0089630.0240810.0186020.0109060.008430.0022910.001733

5010.0089990.0240370.018430.0108170.008350.0022960.001776

5000.0092120.0241260.0187120.0110350.0086010.0025370.001921

4990.0092010.0240620.0186660.011020.0083360.0023670.001799

4980.0091630.024150.0186850.0108890.0085520.0024670.001785

4970.009170.0239070.0184880.0109640.0084480.0023630.001724

4960.0093270.0240090.0185740.0109580.0085050.0023630.001676

4950.0092280.0239630.018390.010790.008520.0024960.00162

4940.0092570.0238840.0184450.010830.0084730.0023440.001752

4930.0093150.0238410.0182640.010830.0084160.0022970.001682

4920.0094020.0236710.018210.0108250.0083770.0023070.001606

4910.0093320.0236210.0181590.0106730.0082730.0021890.001653

4900.0092040.0235540.0180170.0107240.0082850.0021470.001573

4890.0093350.0233520.0179990.0106380.0082760.0021140.001436

4880.0092780.0232940.0178290.0104990.008070.0021520.001446

4870.0095630.0232560.0176780.0105530.007970.0022280.001413

4860.0096740.0230560.0178440.0104750.0081520.0021750.001484

4850.0094870.0229350.0176360.0104910.0081050.0020380.001413

4840.009490.0226830.0174050.0102870.0078530.0019110.001271

4830.0095010.0225280.0172610.0102010.0078640.0018590.001281

4820.0095490.0223870.0171560.0101760.0077960.001920.00121

4810.0095590.0221750.0169660.0101050.0076720.0018120.00121

4800.0095160.0220520.0168190.0100420.0077010.0017310.001106

4790.0094510.021640.0165140.0096820.0074140.0015750.001026

4780.0094030.0214820.0163430.0095810.0073660.0015330.001064

4770.0093940.0212460.0161050.0094810.0071130.0014090.001002

4760.0095140.021160.0161180.0095140.0071260.0013760.000936

4750.009510.0208150.0158890.0093210.007060.0013860.000865

4740.0095830.0206870.0157230.0091970.0070410.0013430.000714

4730.0093640.0202840.0153130.0089540.0067080.0010410.000553

4720.0095710.0201140.0152480.0089530.0066980.0010450.000619

4710.0094430.0197540.0149490.0087780.0064690.0009130.000487

4700.0095580.0196420.0147860.0086370.006310.000880.000463

4690.0094440.0192960.0146130.0083930.0062970.0006760.000359

4680.0094060.018940.0142760.0083690.0061250.0005530.000307

4670.0094090.0187290.014220.0082130.0060420.0005960.000331

4660.0095240.0185350.0138720.0080480.0059650.0004820.000175

4650.0092830.0181580.0136880.0079290.0057830.0003070.000095

4640.0095130.0179920.0135150.0077830.0057250.000180.000024

4630.0093210.0175660.0132290.0076210.005529-0.000005-0.000184

4620.0092960.017270.0129650.0075140.0053950.000014-0.000222

4610.0094180.0171040.0127810.0073470.005242-0.000123-0.000444

4600.0093890.016780.0125530.007040.005171-0.000264-0.000416

4590.0093980.0165240.0124210.0070730.004955-0.000255-0.000486

4580.0095050.0161870.0120870.0069680.004889-0.000368-0.000529

4570.0094430.0160550.0119080.0067660.004784-0.000482-0.000534

4560.0094680.0157870.0117920.0066280.004699-0.000397-0.000713

4550.0094390.0154550.0115940.0064460.004622-0.000652-0.000666

4540.0094650.0152510.0113630.006480.004551-0.000699-0.000586

4530.0095510.014920.0111530.0062720.004319-0.000742-0.000775

4520.0094780.0147140.0109250.0061810.004295-0.000709-0.000808

4510.009430.0143550.0106020.0059830.004159-0.000921-0.000888

4500.0093970.0140990.0103890.0057940.003951-0.001045-0.001068

4490.0095290.0138960.010410.0057320.004057-0.001017-0.000946

4480.0095890.0136220.0100390.0057270.003692-0.001225-0.001168

4470.0092740.0131770.0097740.005320.003456-0.001392-0.001354

4460.0095020.0130420.0095840.0052510.003425-0.001473-0.00136

4450.0093930.0128170.0093970.0050570.003332-0.001559-0.001559

4440.0094820.0125620.0091660.0049840.003276-0.001674-0.001598

4430.0095030.0122130.0090120.00480.003125-0.00176-0.001561

4420.0094120.0121190.0088480.0047840.002898-0.0017-0.001799

4410.0095180.0118250.0086040.004670.00287-0.001852-0.001848

4400.0094520.0115360.0084750.0043990.00268-0.002204-0.001901

4390.0094570.0112970.0081630.004190.002687-0.00222-0.002168

4380.0095190.0109450.0079240.0041340.002515-0.002268-0.002154

4370.0093770.0108470.0076990.0038950.002224-0.002416-0.002355

4360.0095370.0106310.0076780.0039980.002138-0.002621-0.002346

4350.0095910.0103780.0073910.0036820.002029-0.002689-0.002466

4340.0094780.0102640.0073350.0035770.001943-0.002813-0.002448

4330.0097130.0101620.0072140.0036260.001982-0.002723-0.002372

4320.0095910.0099520.0070480.0035020.001781-0.002701-0.002487

4310.0096570.0095990.0068030.0033880.001782-0.002882-0.002631

4300.009670.0095480.0067170.0033020.001571-0.002869-0.002612

4290.0095070.0091750.0064670.0030960.001577-0.003103-0.002903

4280.0096020.0090250.0063760.0030250.001452-0.00327-0.002994

4270.0097090.008830.0061140.0029520.001471-0.003198-0.002828

4260.0097240.0087910.0061330.0029470.001346-0.003336-0.002904

4250.0096110.0085110.0059760.0027950.001131-0.003508-0.003152

4240.0097950.0084160.0057160.0025260.001059-0.003576-0.003196

4230.0097470.0082460.0056720.0024630.001021-0.0035-0.003153

4220.0098870.0081510.0055910.0026180.000964-0.003435-0.003145

4210.0099070.0080490.0054950.0025030.00106-0.00353-0.003131

4200.0099020.0079030.0053740.0024260.000935-0.003597-0.003207

4190.0100790.0079120.0053980.0024350.000973-0.003582-0.003216

4180.0099310.0076380.0051480.002330.00083-0.003688-0.00337

4170.0101420.0076280.005090.0022720.000897-0.003783-0.003161

4160.0101570.0075310.0050660.0022150.000873-0.00375-0.003232

4150.0101970.0074480.0049550.0023160.000801-0.003788-0.003308

4140.010260.0073050.0048740.0020860.000715-0.003861-0.003295

4130.0102670.0071940.0047590.0020280.000475-0.003971-0.003367

4120.0102390.0070010.0046240.0019470.000485-0.0041-0.003568

4110.0103830.0069280.0046240.0019320.000595-0.004006-0.003406

4100.0103520.0068820.0044850.0019280.000451-0.004069-0.003569

4090.0105530.0069160.0045580.0019280.000456-0.003964-0.003436

4080.0104860.0068190.0044660.0019620.000538-0.00405-0.003384

4070.0106580.0066960.0043760.0017510.000432-0.004066-0.003567

4060.010740.006880.0044870.0019440.000605-0.003952-0.003423

4050.0107240.0067750.0044840.0019680.000428-0.004196-0.003525

4040.0107420.0066010.004170.0017080.000341-0.004202-0.003649

4030.0108030.0065250.0042820.001680.000399-0.004279-0.003603

4020.0108730.0065790.0041910.0017910.00038-0.004265-0.003655

4010.0108810.0065270.0042210.0017140.000317-0.004314-0.003737

4000.010880.0064420.0040670.0015650.000289-0.004367-0.003729

3990.0110270.0064680.0040880.0015510.000226-0.004354-0.003797

3980.0110870.0065660.0042530.0017160.000241-0.004302-0.003731

3970.0113050.0065520.0041760.0017590.000395-0.00435-0.003779

3960.0113730.0065840.0041190.00160.000313-0.004238-0.003603

3950.0113830.0065130.0040620.0016450.000347-0.004239-0.003777

3940.0112910.0065880.0040870.0017270.00027-0.004292-0.003672

3930.011490.006520.0040390.0016640.00026-0.004302-0.003706

3920.0116220.0066150.0041180.0016790.000284-0.00437-0.003702

3910.0114770.006490.0041930.0016560.000308-0.004391-0.003723

3900.0117810.0066770.0041450.0018640.000434-0.004349-0.003762

3890.0117760.006630.0041170.001710.000439-0.004436-0.003792

3880.011810.0064620.0040750.0016720.000328-0.004481-0.003812

3870.0119480.0066470.0041820.0018410.000323-0.004333-0.003635

3860.0120850.0066980.0041630.0017930.000376-0.004267-0.003708

3850.0120380.0067190.0041150.001740.000391-0.004344-0.003733

3840.0123630.00680.0042740.0017770.000498-0.004294-0.003638

3830.0123660.0067830.0040010.0019030.000312-0.004298-0.003631

3820.0123180.0067860.004120.0018130.000327-0.004376-0.003656

3810.0125250.006820.00430.0019370.000502-0.004282-0.003375

3800.0126780.006910.0042440.0018530.000487-0.004063-0.003566

3790.0126360.0069170.0041420.0018440.00039-0.004435-0.003606

3780.0128490.0071270.004370.0020010.000729-0.004062-0.003253

3770.0128260.0072060.0043920.0020590.000622-0.00417-0.003352

3760.012810.0071270.0043230.0019020.00051-0.004136-0.003482

3750.0130660.0072380.0044640.0021310.000539-0.004091-0.003452

3740.0131950.0070620.0044860.0020960.000655-0.004008-0.003493

3730.0132060.0073410.004480.0022120.00067-0.004141-0.003478

3720.0134770.0073520.0044210.0021050.00068-0.004252-0.003444

3710.0133450.0074150.0044650.0020610.000582-0.004141-0.003526

3700.0134750.0076040.0046450.002250.000708-0.004072-0.003467

3690.0135460.0074520.0045910.0021920.000815-0.004106-0.003342

3680.0136760.007580.0046990.0021720.000767-0.004034-0.003385

3670.0137740.0076830.0046050.0023140.000786-0.004082-0.003438

3660.0140310.0077790.0047460.0024150.000897-0.003999-0.003307

3650.013870.0076630.0047470.002260.000723-0.00401-0.003313

3640.014140.0079270.0048090.0023810.001053-0.004003-0.003302

3630.0142570.0079530.004790.0024590.000975-0.003985-0.003249

3620.0145230.0080640.0050630.0025560.001053-0.003753-0.003162

3610.0144660.0080850.0049470.0025810.001101-0.003859-0.003086

3600.0146190.0080170.0049820.0025080.001092-0.003918-0.00324

3590.0145940.008190.0050260.0025910.001048-0.003884-0.003048

3580.0148120.0082530.0051380.0026930.001204-0.003816-0.002965

3570.0149270.0083670.0051180.002830.00134-0.003653-0.002965

3560.0151720.0084580.0052130.0027520.001204-0.003726-0.002947

3550.0150660.0084310.0050920.0028160.001209-0.003693-0.003001

3540.0152730.008550.0052490.0029240.001282-0.003646-0.002803

3530.0154350.0087190.0055290.0029340.001477-0.003458-0.002741

3520.0153170.0085650.0051820.0028010.00122-0.003828-0.003067

3510.0155770.0085030.0053060.0029330.001328-0.003704-0.002904

3500.0156910.00870.0054070.0029590.001372-0.003657-0.002968

3490.0158240.0089230.0055520.002990.001568-0.003457-0.002641

3480.0157630.0085120.0052880.0028860.00115-0.003924-0.003016

3470.0159710.0088330.0053390.0030140.00137-0.003564-0.002781

3460.016060.0087390.0053850.0029640.001474-0.003703-0.002764

3450.0160840.0087060.0054490.003040.001368-0.003653-0.002849

3440.0163340.0087150.0052810.002910.00134-0.003705-0.002973

3430.0165810.0089360.0055490.0030530.001438-0.003478-0.002814

3420.0166980.008780.0055460.0030990.001596-0.003673-0.002844

3410.0166730.0087950.0053370.0028990.001391-0.003675-0.002807

3400.0169050.008610.0052060.0029790.001421-0.003677-0.003056

3390.0169150.008460.0051640.0028280.001363-0.004019-0.002883

3380.0171170.0086990.0054360.0028620.001416-0.003645-0.002824

3370.0171560.0088080.005280.0029080.001358-0.003778-0.002962

3360.0175330.0088150.0052570.0030070.001398-0.003711-0.002957

3350.0175480.0087030.0053380.002870.001256-0.003741-0.003031

3340.0177620.0088450.0054280.0030240.001463-0.003912-0.003003

3330.017680.008730.0051340.0029420.001208-0.003943-0.003316

3320.0179310.0087710.0052130.0028870.00106-0.003987-0.003156

3310.0180430.008710.0052770.0029960.001311-0.003953-0.003146

3300.0182430.0086450.0051930.003050.001306-0.004016-0.003097

3290.0184730.0086930.0052520.003020.001497-0.003865-0.002795

3280.0184820.0086250.0050970.0029430.001258-0.003863-0.002948

3270.0186830.008550.0054230.002990.001419-0.003684-0.002834

3260.0185360.0084030.0049470.0028090.001373-0.003688-0.002781

3250.0187510.0087510.0050340.0027050.001485-0.003504-0.002741

3240.0192380.0087680.0050540.0030970.001646-0.003543-0.002839

3230.0193570.0087020.0051570.0028080.001572-0.003345-0.002645

3220.0192550.0086690.0050720.0028440.001574-0.003801-0.002811

3210.0193970.0083990.004850.0025940.001451-0.003549-0.003013

3200.0195880.0086730.0051380.0028380.00147-0.003755-0.002781

3190.0196940.0082210.0051210.0028440.001525-0.003559-0.002763

3180.0199770.0085780.0050810.0029170.001544-0.003703-0.002666

3170.0198570.0086460.0050950.0028780.001588-0.003553-0.002825

3160.0207330.0087770.0055960.0030730.001851-0.003196-0.002486

3150.0206040.0086450.005390.0031510.001822-0.003423-0.002626

3140.0205460.0087380.0052290.0029080.001383-0.003694-0.002903

3130.0208770.0087380.0053430.0029520.001378-0.003381-0.002748

3120.020990.008780.0055280.0032010.001578-0.003578-0.002724

3110.0214440.0088990.005450.0030930.002032-0.003534-0.00267

3100.0214470.0089710.0053260.0029590.001677-0.003774-0.002905

3090.0218140.0092970.0056470.003260.002242-0.00338-0.002497

3080.0219410.00920.0054510.0033490.001852-0.003545-0.002628

3070.0222010.0097720.0059160.0035680.002119-0.00323-0.002452

3060.0223910.0100580.005910.003660.002251-0.003268-0.00221

3050.0227030.0105210.0064870.0040130.002691-0.002988-0.002022

3040.0229550.0112810.0069150.0043950.002905-0.002669-0.001973

3030.0231630.0119390.007650.0047720.003448-0.002524-0.001658

3020.0236650.0128810.0081670.0050580.003537-0.002215-0.001557

3010.0238750.0137210.0091090.0059020.004187-0.001614-0.000927

3000.0243370.0144150.009590.0061710.004582-0.001475-0.000723

2990.0247820.0154420.0104660.0066250.005133-0.001237-0.000427

2980.0252880.0167720.0113120.0074590.005703-0.0007190.00001

2970.0257820.0178550.01220.008090.006202-0.0002580.00054

2960.0265550.0194780.0132950.0090660.0071890.000190.00109

2950.0272460.0210820.0146970.009910.0077270.0010860.001593

2940.027750.0233480.0164850.010970.0090030.0016820.002063

2930.0284530.0261760.0182180.0125630.0102530.0026370.002985

2920.0298070.0288690.0205180.0141070.0116480.0037520.004101

2910.0302720.0312550.0223280.0156420.0129330.0046570.00482

2900.0310130.0332370.0240460.0166980.0138060.0051270.005388

2890.0318220.0356370.0256180.0179330.0148190.0062170.006163

2880.032530.0383770.027820.0193610.0163830.0071890.006906

2870.0335980.0414490.0301980.0211250.0180140.0085180.00824

2860.0343670.0434490.0318650.0223920.0188770.0090520.008833

2850.0353050.0454550.0329090.0235390.0199290.0098180.009454

2840.0359060.0464330.0339450.023950.0205710.0100960.010012

2830.0366770.0474780.0347340.0244270.021020.0106350.010405

2820.0375210.0478940.0350280.0247590.0212710.0108030.010548

2810.0383450.0486640.0354090.025330.0216340.0111050.010885

2800.0393580.0490750.0361030.0256640.0220690.0112920.010982

2790.0404050.0495950.0362020.0257330.0221350.0112390.011325

2780.0414650.049440.0363240.0259460.0224660.0113530.011463

2770.042330.049430.0362820.0257130.0222250.0114230.011523

2760.0427440.0487760.0353630.0251740.0217710.0110670.011047

2750.0433830.0483550.0355060.0253610.021990.0113110.011576

2740.0437040.0474670.0347520.0250250.0219940.0109780.011313

2730.04430.0471740.0345370.0249680.0217850.0110180.011063

2720.0443140.0458180.0333290.0239220.0210780.0103390.010918

2710.0449470.0450440.0326240.0238350.0208510.0102010.010764

2700.0453160.0444470.0324240.0233730.0205870.0100090.010663

2690.0456260.0437270.0318740.0230780.0200910.0097670.01059

2680.0454620.042570.0311080.0223670.0197620.009380.010451

2670.0452520.0414470.030020.0218480.0192570.0090270.010121

2660.0451280.0403110.0295320.0213720.0190650.0086810.009863

2650.0450410.0396510.0286630.0208780.0185130.008530.009433

2640.0450780.038210.0278870.0202080.0180160.0078160.009128

2630.0450130.0368910.026740.0194170.0174070.00740.008864

2620.044750.0359640.0260310.0190440.0169760.0070990.008472

2610.0443580.0353220.0256260.0185870.0167950.0070810.00861

2600.044270.0346550.0250540.0182420.0164490.0068490.008332

2590.0441440.0341890.0247650.0179990.0161980.0063520.008024

2580.0437550.0329990.0237620.0172580.0156170.0059540.007588

2570.0439410.0320310.0231570.0169070.015030.0056670.007234

2560.0434420.0310770.0225150.0162360.0145820.0051280.006796

2550.0433470.0308210.022110.0161280.0143320.005040.007019

2540.0432770.030780.0220890.0161110.0143750.004960.006693

2530.0435470.0310350.0222930.0162630.0146280.0051250.006895

2520.0435810.0309680.0221740.0161080.0144340.0051180.00681

2510.0433150.0306160.0219230.0158130.0141160.0048730.006559

2500.0435140.031010.0222910.0161940.0145120.0051080.006905

2490.0435960.0319380.0230070.0168190.0151010.0054740.007092

2480.0435130.0332110.0238580.0171810.0155660.0057050.007596

2470.0438720.0347520.0251950.0182270.0162560.0064740.00803

2460.0440560.0368010.0269280.019360.017170.0072990.008481

2450.0444310.0397450.0289430.021170.0186830.0082980.009304

2440.0448450.0432610.0316410.0228440.0203140.0094120.01046

2430.0456170.0481890.0354670.0256750.0227330.0111710.012075

2420.0462630.0534690.0397610.0287480.0256510.0133620.014126

2410.0476330.0608230.0453090.0329610.0290950.0160940.016623

2400.048640.0693150.0521670.0378260.0335530.0191720.019287

2390.0501940.0803620.0605940.0440340.0389760.0233210.023041

2380.0521210.0940540.0711340.0515930.0455020.0282990.027662

2370.0538350.1097310.0834240.0603110.0533220.0341850.032731

2360.0563320.1283960.0981150.0709720.062610.0412670.038983

2350.0587790.1496950.1146750.082980.0732960.0489670.046151

2340.0612860.1728040.1326130.0961450.0848730.0577190.053831

2330.0635390.1965710.1512520.1095910.0964510.0666770.061738

2320.0650950.2198490.1694890.1228440.1082840.0751080.069573

2310.0661460.2417670.1866590.1354050.1193910.0835780.077366

2300.0659170.2607270.2016480.1464670.1290470.0907410.084185

WT 1

Fractions

Relative Ratio A280/A500

(D) Wild type rhodopsin

WT

Wavelength (nm)

Absorbance

Spin labeling experiment for electron paramagnetic resonance (EPR) spectroscopy Rhodopsin was labeled with 50uM NEM in 50uM NaPi, 0.05%DM in dark for at least 16 hrs Wash with 5mM MES, 0.05%DM 50uM spin label MTSL was added for 3 hrs RT

Rhodopsin was labeled with 50uM NEM in 20mM Tris, 0.05%DM, 150mM NaCl in dark for at least 16 hrsWash with 20mM Tris, 0.05%DM, 150mM NaCl50uM spin label MTSL was added overnight at 4C R9 and R11After extensively washing with 1XPBS, 0.05%DM followed by 2mM NaPi, 0.05% DM of solubilized Rhodopsin-1D4 SepharoseWashing 5mM MES, 0.05%DMLabeled Rhodopsin were eluted 70uM epitope nonapeptide

Chart2

-0.002806-0.001931-0.0022550.0012670.0005640.0035270.0045310.0084590.003624

-0.002743-0.001945-0.0021960.0012720.0005050.0035270.0046710.0084940.003611

-0.002748-0.001908-0.0021090.0012720.0006240.003620.0046430.0084520.003583

-0.002803-0.001991-0.0022330.0012310.0005650.0035190.0045840.0083750.003598

-0.002662-0.001826-0.00210.0012630.0006340.0036580.0047040.0084770.003699

-0.002658-0.001877-0.0021190.0012640.0006570.0036540.0047010.0083810.003682

-0.002626-0.001804-0.0020230.0013140.0007210.0037790.0047650.0085580.003774

-0.002695-0.001955-0.0021330.0011630.0006150.003650.0047340.0084290.003655

-0.002586-0.00191-0.0020650.0012640.0006380.0037060.0047720.0084770.003766

-0.002622-0.001901-0.0020880.0012320.0006250.0038220.0047860.0084360.003697

-0.0026-0.001869-0.0021110.0011950.0006250.0038410.0047220.0084190.003735

-0.002591-0.001782-0.0020290.0013380.0007540.0039250.0048570.0084940.00373

-0.002564-0.001838-0.002020.0012880.0007350.0039490.0047840.0084670.003773

-0.002542-0.001893-0.0021120.0012470.000680.0038520.0047660.0083990.003718

-0.002492-0.001778-0.0020030.0012930.0007590.0040010.0049190.0083660.003811

-0.002538-0.001816-0.0020630.0012510.0007270.0039830.004860.0084620.003835

-0.002424-0.00182-0.0020260.0012750.0007270.0039890.0048610.0083510.003785

-0.002498-0.001821-0.0021270.0012110.0007180.0039850.0048670.0083250.003758

-0.00237-0.00183-0.0019860.0012750.0007820.0040960.0049510.0083950.003818

-0.002361-0.001762-0.0019860.0013030.0008420.0040780.0049280.008420.003869

-0.002375-0.001748-0.0021050.0012890.0007550.0040980.0049480.0083610.003843

-0.002261-0.00167-0.0019360.0013820.0009070.0042140.0049940.0084220.003907

-0.002275-0.001712-0.0019550.0013310.0008150.0042840.0050610.0083770.003941

-0.002193-0.001685-0.0019640.0013680.0008790.0042750.0050240.008420.003946

-0.002193-0.001607-0.0020010.0013270.0008470.0042760.0050010.0083420.0039

-0.00212-0.001593-0.0019280.0014280.000930.0044250.0050570.0084120.003956

-0.002107-0.001621-0.0018690.0013780.0009440.0043880.0051230.0083380.003915

-0.002006-0.001534-0.0018410.0014380.0009860.0044490.0051650.0083010.004026

-0.001997-0.001497-0.0018140.0014840.0009950.004510.0052120.0084090.004026

-0.002039-0.001534-0.0018190.0014750.0010090.0044820.0051340.0083170.003971

-0.00192-0.001406-0.0017180.0015580.0010410.0045150.0052790.0083310.00405

-0.00175-0.001305-0.0016720.0016040.0011750.0046730.0052740.008420.004083

-0.001805-0.001383-0.0016910.0015540.0010880.0046840.0052470.00830.004074

-0.001732-0.00136-0.0016720.0015860.0011340.0046790.0053030.0083140.004075

-0.001581-0.001273-0.0016360.0016790.0011850.004740.0053220.0083620.00408

-0.001581-0.001177-0.0016090.0016420.0011570.0047310.0053320.008270.00408

-0.001402-0.001177-0.0015120.0017120.0012360.004820.0053510.0082750.004057

-0.001384-0.001049-0.0015310.0017350.0012820.0048860.0053280.0082570.00416

-0.00126-0.000966-0.0013750.0018230.0012550.0049940.0054180.00830.004165

-0.001104-0.000865-0.0013930.0019250.0014070.0050130.0055250.0082820.004263

-0.00098-0.00075-0.0012240.0019580.00150.0051760.0055770.0082970.004273

-0.000847-0.000645-0.0012840.0020270.0014910.0051310.0055830.0083030.004232

-0.000727-0.000566-0.0011370.0020560.0014910.005220.005640.0083140.004307

-0.000516-0.00035-0.0010170.0021210.0016210.0053420.0057580.0083440.004336

-0.000373-0.000198-0.000880.0022420.0017370.0053950.0057870.0084440.004397

-0.000175-0.000124-0.0008390.0022750.0017750.0054950.0058030.0083720.004371

0.0000090.000074-0.0007510.0023870.0018720.0055290.0058930.0083550.004446

0.0002820.000189-0.0005630.0025270.0019650.0056280.0059930.0084890.004536

0.0004760.000457-0.000360.0026530.0021330.005840.0060880.0085570.004579

0.0006790.000568-0.0002720.0027050.0021760.0058990.0061660.0085230.004581

0.0009390.000767-0.000180.0028170.002260.0059580.0062340.0085930.004616

0.0012260.0010130.0000420.0030050.0024050.0061610.0063540.0086240.00472

0.0014080.0011390.0000920.0029970.0024950.0061660.0063580.008630.004681

0.001710.001390.0003420.0032320.002580.0063510.0064590.0086860.004763

0.0019670.0016510.0004210.0033270.0026790.006450.0065670.0086870.004869

0.0023410.0018750.0005930.0035020.0027930.00660.0067080.0087780.004971

0.0024920.0020490.000770.0035520.0029820.0066570.0067880.0088230.004895

0.0029140.0023680.001050.0038390.0031560.0068730.0068870.0089230.005053

0.0032620.0027150.0012040.0039270.0032250.0069870.0069540.0090540.005142

0.0035650.0029610.0013640.0040990.0033440.0070970.0070970.0090.005174

0.0039430.0032210.0016030.0043320.003530.0072490.0071880.009150.005277

0.0042610.0034350.0017490.0043790.0035760.0072660.0072040.009130.005278

0.0046550.0037430.0020080.0046130.0037660.0074710.0074090.0093370.005282

0.0051210.0041220.0023180.0048570.0038820.0076090.0074710.0093490.005437

0.0055540.0045160.0025360.005040.0041340.0077810.0076240.0094610.005512

0.0061020.0048350.0028340.0053030.0043160.0079670.0077820.0095440.005582

0.00660.0051990.0031090.0054540.0044520.0080260.007850.0095610.005686

0.0070030.0056370.0033880.0057080.0047330.0082730.0080110.0096670.005807

0.0075030.0059690.0036320.0059160.004780.0083470.0081270.0096990.005741

0.0082060.0065540.0040390.0063170.0050220.0085970.0082820.0099790.005999

0.0088260.0070610.0043620.0064860.0053330.0088160.0084720.0100940.006092

0.0094990.0075070.0048040.0067970.0055340.0089770.0086040.0102080.006137

0.0101410.0080450.0051140.0071290.0057690.0091920.0088380.0103080.006306

0.0109020.0086340.0056280.0075260.0059460.0094330.0090260.0105170.006369

0.0116980.0092480.0060420.0078520.0063230.0096650.0092150.0106440.006551

0.0125390.0099510.0065340.0082130.0066050.0098590.0094330.0107380.006677

0.0132910.0104430.0069590.0086070.0068640.010150.0095690.0109230.006826

0.0141220.011080.0072990.0089050.0071660.0103390.0096770.0109740.006856

0.0150670.0119310.0080240.0094310.0075420.0106360.0100360.0111990.007074

0.0161170.012660.0085550.0098490.0079340.0109490.0103140.0114640.007275

0.0171430.0135220.0091110.0102580.0082740.0113020.0105170.011610.007419

0.0181480.0142870.0096860.0107960.0085990.0115750.0107380.0117060.007509

0.0192270.0151760.0102720.0112920.0089530.0118510.0110180.0119710.007691

0.0203330.0160280.0109890.0118370.0094330.0121840.0112930.0121750.007844

0.0216390.0170730.0117770.0124330.0098630.0125730.0115460.0124620.008053

0.0228080.0180240.0125240.0130030.0103290.0129930.0118780.0126310.008364

0.0241220.0189460.0131440.0135260.0107860.0133180.0121780.0129120.008493

0.0254550.0200520.0140040.0141390.0112420.0137040.0125920.0132250.008818

0.0267450.0210810.0147460.0147410.0117810.0141350.012790.0134090.008875

0.0282630.0222180.0155950.0155030.0121950.0144880.01320.0136790.00909

0.0296880.0233660.0163010.0160970.0127460.0148620.0133940.0139170.009297

0.0312960.0245990.0172620.0168380.0132650.0154170.0138410.0142380.009582

0.0328470.0258970.0182490.0175690.0138760.0158470.0143220.0144960.009801

0.0345320.027220.0192130.0185070.0145670.0165230.0147710.0149360.010192

0.0362330.0285560.0201760.0191790.0151790.016850.0150580.0152130.01037

0.0379210.0298730.0211190.0199480.0157610.017390.0154790.0155130.010662

0.0396440.0312320.0221850.0208050.0164010.0179050.0159830.0159340.010987

0.0413070.0325290.0231470.0215810.0169980.0183430.0162820.0161010.011263

0.0430840.0340030.0242340.0224020.017640.0189270.0167760.0165370.011463

0.0449330.0354880.0252290.0232790.0182590.019440.0171840.016930.011814

0.0468420.0368190.0263790.0241270.0189550.0200240.0175890.0172380.012084

0.048680.0383810.0274750.0250470.0196590.0205880.0180960.0176460.01243

0.0505980.0398120.0285650.0258380.0203660.0210940.0185550.0180030.0127

0.0525790.0413990.029640.0267620.0210670.0216630.0190530.0183090.012979

0.0544650.0428740.0307480.0276620.0217190.0221230.0194410.018760.01326

0.0564670.0444930.0319510.0286170.0224290.02280.0200060.0191480.013639

0.0584360.0460660.0331610.0295670.0231390.023470.0204470.0194840.014045

0.0604270.0475550.0342660.0304920.0239260.0240060.0209680.0198820.014248

0.0625060.049190.0354340.0314030.0245810.0245810.0213720.0202010.014602

0.0644010.0507630.0366120.0322910.0253160.0251920.0219340.0206390.014966

0.0664750.0523010.0377710.0332610.0260650.0257660.0223950.0209710.015303

0.0685730.0540510.0390020.0342930.0268110.0264170.0229370.0213970.015598

0.0705470.0555370.040070.0352060.0275380.0269680.023350.0217550.015911

0.0725070.0571460.0412410.0361240.0282540.0275640.0238610.0222730.016233

0.0744210.0587180.0423710.0370260.0289840.0281420.0243550.0225820.016537

0.076440.0601680.0435340.0379020.0296880.028730.0248480.0229540.016859

0.0784430.0618060.0447460.0389660.030420.029350.0253480.0234080.017259

0.0805070.0633580.0458490.0398640.0311440.0299260.025850.0238040.017629

0.0823430.0649470.0469970.0407430.0318740.0304830.0263010.0241030.01789

0.0843250.0663240.04820.0416140.0325640.031040.0267420.0244780.018126

0.0862330.0680190.0492810.0425910.0333090.0316970.0273130.0249770.018575

0.0880990.0694270.0503490.0433870.0339310.0322450.0276460.0253120.018811

0.0899530.0708550.0514140.0442540.0346010.0327910.0281510.025640.01914

0.0916260.0721870.0524580.0451290.0352820.0332820.0285860.0259230.019368

0.0934460.0734940.0534630.0458670.0358920.0337770.0290760.0263150.019691

0.0950760.0748040.0544240.0467120.0364660.0342620.02950.0266520.020012

0.0964850.0760250.055210.0473550.0369390.0347360.0297360.0268510.020206

0.0981840.0773440.0561650.0481210.0375890.0352920.0302510.0272620.020484

0.0997070.0785350.0570760.0488460.0381680.0356130.0306230.0274510.020659

0.1011870.0797420.0580110.049470.0387610.0361470.031050.027850.021037

0.1026670.0808260.0587010.0501230.0392850.0365540.0313660.0280990.021183

0.1039960.0819340.059520.050830.0397310.0369210.0316380.0282920.021412

0.1052620.0829270.0602960.0513960.0402410.0373560.0320570.0286430.021678

0.1064780.083830.0610110.0519640.0406450.0376440.0322660.0288650.021748

0.107610.084750.06170.0525420.0410590.0380910.0326360.0290520.022055

0.1088810.0857540.0623960.0531730.0415720.0385390.0329890.0293570.022278

0.1097370.0865180.0629010.0536080.0418750.03880.0331790.0295810.022394

0.1107820.0871370.0634660.0539310.0421520.0390430.0333930.0296920.022536

0.1117590.0881010.0641120.0545430.0426510.0393950.0337460.0300430.022814

0.1124220.0886520.06450.0547330.0428110.039620.0338040.0300130.022871

0.1132960.0892970.0650640.0551950.0431530.0398670.034150.0302610.023012

0.1139990.0899830.0653550.0555960.0434850.0400830.0343320.0303550.023113

0.1147170.0904490.0658340.0558720.0437590.040350.0345040.0305960.023306

0.1153210.0908560.0661950.0562080.0440120.0405130.0347560.0306290.023486

0.1157910.0913260.0664370.0563790.0442070.0407160.0348740.0307150.023471

0.1162770.0915490.0667490.0565370.044250.0409080.0350520.0308360.023563

0.1167340.0919970.0669950.0568360.0444350.0410960.0352130.0309490.023644

0.1170980.0923040.0671680.0569780.044750.0411920.0353520.030960.023745

0.1173060.0924870.0673280.0571440.0447590.041350.0354570.0310590.023831

0.1175170.0927050.0674030.0571620.0448730.041380.0354750.0310960.023791

0.1176420.0927150.0674910.0572620.0449220.0414170.0355310.0310960.023726

0.1178410.0928660.0675930.0573320.0450020.0415750.0357090.0312460.02391

0.1178560.0928610.0676260.0573790.0450830.0416350.0357310.0312590.024046

0.1177320.0928340.067520.0572530.0450220.0417180.0357210.0312430.023979

0.1176150.0927620.0674390.0572420.0450210.0416760.0357810.0311650.023882

0.1174260.0925790.0673380.0571330.0450660.041690.0358560.0311490.023877

0.1170650.0924170.0671670.0571510.0449130.0416150.035750.0310980.023864

0.1168990.092280.067080.0570780.044870.0416090.035790.0310780.023955

0.11650.0919790.0667190.056830.0448030.0415620.0357230.031010.023856

0.1160250.0915190.0664660.0566820.04460.0414210.0356850.0309460.023797

0.1156180.0913090.0662680.0564190.0446880.0414210.0356790.0309150.023822

0.1150350.0907630.0659480.0561950.0444350.0413140.035450.0307480.02361

0.1144870.0903630.0655840.0559080.0442430.041190.035520.030650.023593

0.1137930.0898290.0651460.0555090.043930.0409720.0354570.0305110.023529

0.1131990.0894190.0648630.0553560.0438440.0409690.0353940.030590.023488

0.1124070.0887460.064420.0550390.0435340.0407790.0353020.0304230.023412

0.1116410.0881910.0639530.0546390.0433410.0405510.0351530.0303310.02338

0.1107640.0874950.0635040.0542090.0429520.0404120.0349940.0301180.023239

0.1099270.086810.0629630.0538550.0427510.0402230.0348590.0300230.023185

0.1090140.0860120.062380.0534060.0424890.0399880.0347640.0298790.023048

0.1080770.0853970.0618240.0529780.0421750.0398260.0346230.0296790.023009

0.1070510.0845150.0612370.0525390.041750.0394740.0343820.029570.022757

0.1060490.0837730.060630.051890.041370.0391730.0340690.029250.022515

0.1049420.0828850.0599750.0514810.0410210.0389450.0338930.0291270.022463

0.1038390.0820620.0593540.0509720.0406530.0386290.0337490.0289030.022296

0.1026520.081160.0586810.0505230.0403590.038440.0335360.0287980.022118

0.1014860.0802920.0579850.0499180.0399120.0381390.0333910.0285890.022041

0.1002250.0792250.0573020.0493840.0395180.0378910.0331450.0283050.021884

0.0990250.0783690.0565780.0488430.0391530.0376040.0329320.028170.021766

0.0978080.077370.0557930.0482080.0387020.0373030.0327410.0279350.021495

0.096470.0762760.0550390.0475970.0382220.0368910.0324650.0277120.021363

0.0951030.0753380.0542810.0471360.0379310.0365910.0322750.0275190.021248

0.0939170.0743650.0535170.0464420.0374770.0363270.0320190.0272250.021101

0.0925740.0733360.0528290.0459070.0370780.0359960.0318140.0271240.020923

0.0911780.0721920.0519030.045110.0365110.035660.031440.0267930.020646

0.0897560.0711820.051180.0445090.0361470.0353340.0313040.0266040.020539

0.0883680.0700550.050250.0438780.0355890.0349660.0310550.0263120.020305

0.0871320.0690050.0495440.0433720.0351440.0346750.0308030.0260830.020154

0.0857320.067950.0487590.042660.0347990.0343090.0306680.0259460.020048

0.0843550.066960.0478820.0420750.0343050.0339280.030350.025770.019795

0.0830010.0659070.0472540.0414290.0338750.0337070.0300750.0254590.019666

0.081680.0648320.0463580.0407620.033360.0332530.0298620.0250770.019397

0.0802680.0637640.0456120.0401810.0329980.0329520.0296190.0249570.019357

0.0789240.0626890.0447280.0395290.0324870.0325780.0294340.0247130.018997

0.0775490.0616860.0440240.0389160.0320620.0324070.0291280.0244210.018936

0.0761270.0606480.04320.0382390.0316070.0320080.0288630.0242270.018704

0.0748970.0595680.0424040.0377610.0311550.0316930.0287050.0240310.018588

0.0736190.0585620.0417190.0371220.0307880.0314010.0285110.0237880.018386

0.0722280.0575320.0408530.0364910.0303560.0311560.0282770.023610.018199

0.0709720.0564080.0401840.0359030.0298090.0307560.0280280.0232720.017961

0.0696210.0555180.0393860.0353360.029540.0304210.0278510.0231440.017822

0.0684290.0546320.0386460.0348060.0291770.0302650.0276890.0229580.01773

0.0670330.0535390.0379330.0341840.0286560.029980.0275310.0227180.017619

0.0655740.0523520.0370050.0335370.0280970.0295580.0270790.0224660.01733

0.0644140.0514380.0362690.0329730.0277390.0293320.0270030.0223140.017266

0.0629960.0503550.0355090.0322260.0273390.0288960.0266690.0220140.01699

0.0617780.0493970.0348210.0317710.0269520.0286050.026610.0219630.016882

0.0604080.0483860.0340060.0311530.0264080.0283380.0263680.0216550.016752

0.0591670.0474460.0332530.0305760.0260670.0280620.0261520.0214290.016589

0.058230.0466540.0326620.0301810.0257350.0279020.0260280.0214840.016588

0.0567390.0455770.0318980.0294810.025270.0274820.0257940.0211290.016203

0.0557460.0449320.0311860.0289750.0250030.0271640.0255780.0210280.016126

0.0545890.0440780.0305990.0284760.0245640.0269610.025390.0207860.016068

0.0534060.0430380.0299290.0281180.0242870.0267750.0253350.0205840.015914

0.0522760.0423130.0293590.0276870.0240140.0265470.0252180.0204020.015871

0.051150.0414350.0288370.0270390.0236870.026350.0250570.0204050.015877

0.0501810.0406030.0280780.0266630.0232770.0261210.0248020.0202410.015579

0.0489420.0398030.0273360.0261090.0228710.0257650.0246740.0199570.015548

0.0481360.0391910.0268870.0256860.0226570.02560.0246160.0199640.015455

0.0470750.0382820.0262560.0252390.0223770.0254260.0244310.0197190.015255

0.0461310.0376320.0257480.0248990.0221290.0250860.0244340.0197220.015232

0.0452210.0368750.0251790.0242490.0216180.0248650.0241030.0194570.015027

0.0444150.0361690.0247460.0239780.021550.0248620.0242050.0193540.015129

0.0434510.0356050.0241740.0235390.0211020.0245680.0239720.0192470.014884

0.0426650.0348460.0237180.0230730.0208240.0243440.0237740.0190440.014692

0.0418790.03440.023350.0228510.0207980.0242320.0237930.0190890.014731

0.0410170.0337710.0228020.0223140.0203020.0241020.0237490.0188830.014678

0.0402630.0332290.0223070.0221010.0201450.0239290.0236110.0188110.014641

0.0394810.0326530.0219610.0216590.019910.0237190.0234010.0187010.014495

0.0389040.0322240.0215930.0214820.0198040.0235910.02350.0186250.01441

0.0381890.0316680.0211590.0210440.0195410.0234690.0233580.0184780.014314

0.037460.0312940.0208640.0207990.0193570.0233480.0233230.0184290.014319

0.0369260.0308380.0204870.0205920.0192210.0233070.0233230.0184370.014287

0.0363450.0304170.02020.02030.0190140.0231750.0232660.0183460.01425

0.0357740.0300380.0198780.0200930.0188780.0230530.0232650.0182240.014149

0.0351440.0296140.0195570.0197970.0186870.0229880.0231740.0181280.014097

0.0347250.0293270.0193050.01960.0186450.0229260.0232190.0181810.01411

0.034240.0290210.0190490.0194590.0184940.0228450.0232180.018140.014132

0.0338620.0287340.0188270.0191870.0183770.0228380.0231920.0181080.014055

0.03340.0284710.0185970.0190020.0182570.0228290.0232230.0180370.014024

0.0330660.0282360.0183910.01890.0181660.0227580.0232070.0180460.014121

0.032710.0280160.0182340.0187830.0181590.0227510.0232870.0180690.014044

0.0324330.0278650.0180450.018630.0181350.0227130.0233030.018150.01409

0.0320340.0276310.0178580.0184420.0179980.0227930.0232830.0180220.013937

0.0318560.0274640.0177020.0183570.0178920.0226920.0233950.0180720.014064

0.0316250.0273580.0176630.0182720.0180120.0228440.0235110.0180620.014074

0.0313610.0272480.0174580.0181530.0178480.022730.0234730.0180930.014088

0.0312670.0272060.0174550.0181850.0179250.0228490.0236430.018210.014125

0.0310720.027160.0173740.0180430.0179430.0228780.0237230.0182230.014236

0.0309910.0270440.0173080.0180520.0178870.0229140.0238150.0181870.014264

0.0309050.0269880.0171970.0180210.0180160.0229440.0239310.0182910.014292

0.0307630.0269780.0171390.0179080.0179330.0230490.0239760.0182480.014268

0.0307350.026940.0170930.0179630.0179780.0231110.0240590.0183880.014316

0.0306420.0269440.0170560.0178950.017980.0231650.0241640.0182760.014368

0.0305910.0269480.0170620.0178310.0179770.0232080.0242020.0184020.014398

0.0306860.0270520.0171060.0179560.0180660.0233660.0243450.0185320.01452

0.0306890.0270740.0170950.0178510.0182360.0234430.024530.0184620.014514

0.030750.027190.0171170.0179980.0183190.0235370.0246750.018690.014715

0.0307130.0271770.017180.0179960.0183020.0236650.0247230.0186030.014563

0.0307470.0273130.0172050.0180060.0184280.0237580.025030.0186940.014731

0.0308070.0273250.01710.0180860.0185130.0239630.0251690.0188640.014829

0.0310420.0275430.0172910.0182430.018740.0240970.025370.0190120.01495

0.0309890.0276170.0172390.0182270.0187090.0242450.0254840.0191110.014982

0.0311650.0276410.0173150.0182930.018720.0241970.0254460.0190160.014941

0.0313510.027960.0173970.018340.0189930.0244630.0258040.0191940.015188

0.0314950.0280450.0175670.0184510.0190540.0246330.0258530.0193310.015222

0.0317270.0281910.0176340.0185140.019260.0247560.0261120.0193350.015164

0.0323320.0288220.0180750.0188220.019570.0254060.0263050.0195450.015333

0.0324320.0287210.0180250.0189640.0197320.025290.0266370.0198130.015467

0.0325950.0289230.0180610.0189130.0199080.0255140.0267730.0197250.015601

0.0328040.0291530.018130.0191690.0199620.0258020.0269830.0198850.015727

0.03320.0292630.0183030.0191340.020160.0259580.027310.0200830.01592

0.0332220.0295060.0184180.019370.0202480.0261770.0273320.0200750.015966

0.0333840.0296790.0184160.0194080.0203110.0262520.0275520.0202050.015966

0.033820.0300040.018790.0196810.0207770.0265050.0279130.0203760.016193

0.0339590.0300690.0186230.0196250.020660.0265580.0279040.0205030.016258

0.0342130.0303480.0188330.0198710.0208410.0267280.0282290.0205870.016292

0.0346040.0305960.0189980.019980.0210360.0269290.0284410.0208480.016487

0.0348790.0307290.0191180.0202880.0212070.0271740.0286350.0209280.016576

0.0351250.031030.0192490.0203130.021380.0273180.0288260.0209280.016677

0.0355810.0314210.0195490.0205420.0216490.0276760.0292110.0211820.016935

0.0358520.0316740.0196950.0207030.0218010.0278040.0293380.0213840.017065

0.0361450.0317970.0197020.0207060.0219760.0279410.0295490.0213810.017092

0.0364060.0319660.0198650.0208950.0220390.0280720.0297630.0215450.017154

0.0367610.0323020.0199150.0211220.0224040.028380.030.0216960.017294

0.0372020.0325790.0201190.0213110.0226140.0286650.0302950.0219420.017613

0.0374790.0328210.0204670.0215840.0227860.0289160.0305940.0221130.017783

0.0376890.0331170.020480.0216880.0229770.0291260.0308570.0222230.017856

0.0383010.033390.0207580.0218550.0232410.0294480.0311290.0225010.018047

0.0385330.0337020.0208420.0220510.0233510.0295350.0313720.0225760.018024

0.0389080.0339840.0209530.0222440.0236060.0298560.0315950.0226570.018221

0.0392650.0342950.0212030.0223480.0237610.0300240.0318470.0228320.018303

0.0396560.0345240.0212820.0224980.0240240.0303070.0320690.0229260.018421

0.0399950.0347480.0215280.0227250.024190.0305750.0323440.0231480.018544

0.0402810.0350090.0216560.0228530.0244060.0308060.0324930.023220.018665

0.0405920.0352520.0217870.023020.0246770.031020.0328280.0234850.01881

0.0409130.0356470.0218520.0231680.024820.0313430.033220.0235760.018995

0.0412950.0357810.0220860.0233460.0251230.0315170.0333640.0237710.019075

0.0415250.0359790.0221180.0235020.0253110.0318670.0337640.0238750.019267

0.0417890.0361210.0221260.0236280.0254850.0321250.0339880.0240940.019339

0.0419840.0362620.0223350.0237060.0256310.0322520.0341160.0242850.01949

0.0418930.036420.0222550.023750.0256920.0323890.0342820.0242470.01961

0.0421630.036540.0223670.0239040.0259680.0327880.0345950.0243970.019674

0.0423090.0365420.0221650.0238260.0259430.0328380.0346830.0243660.019796

0.0423530.0365630.0222070.0237630.0260050.0329030.034930.0245860.019826

0.0424310.0368230.0223540.0240080.0262740.0333270.0351560.0247290.020026

0.0425370.0367420.022370.0240510.0264020.0333850.0355970.0249080.020193

0.0426550.0367710.0222930.0240220.0266180.0337130.0358640.0250540.020321

0.0426080.0367530.0222330.024220.0266880.0337570.0359510.0251650.020367

0.0426690.0369480.0221990.0241140.0268480.0340180.0361940.0251740.020552

0.0427770.0368830.0222120.0242530.0269680.0340470.0364310.0254790.020683

0.0428740.0369770.0222120.0242740.0271150.0344840.0367540.0256090.020667

0.0428210.037030.0221720.0242340.0271380.034710.0369760.0257460.020929

0.0428270.0369530.0220650.0242720.0271360.0347230.0372620.0256860.020914

0.0431280.0369980.0220.0243360.0274660.0350330.0375950.0259840.021126

0.043020.0370510.021910.0243390.0275320.0351750.0377920.026190.021268

0.0427750.0369660.0217890.0243270.0275840.035280.0380110.0261580.021367

0.0428380.0368930.0218010.0241640.027750.0354620.0381840.0262340.021405

0.0426890.0368510.0215380.0239990.0276940.0354940.0383730.0263810.021394

0.0427220.0367290.0215510.0241620.0279150.0357450.0385770.026570.021556

0.0425050.0366880.0214350.0240370.0278910.0358730.0385520.0263780.021481

0.042570.0369410.0214760.0242890.028180.0362320.0391380.0268540.021918

0.0426080.0368990.0215020.0242910.0282950.036190.0394570.0268330.021924

0.0432140.0372630.0212870.0248640.028840.0371150.0399670.0273570.022636

0.0431480.0373640.0210180.024910.0293090.0373530.040190.027160.022609

0.043270.0373320.0212010.0248360.0292120.0376120.0404510.0274370.022976

0.0435560.0374890.0214330.0251260.0294310.0380650.0412410.0278150.023424

0.0427850.0371010.0207940.0245170.0292060.0377630.0407970.0276510.023164

0.0434470.0376370.0213180.025310.0299250.0384950.0414950.0279450.023371

0.0431450.0372520.0208840.0249250.0298190.0385820.0414330.0277450.023166

0.0432320.0374650.0209940.0249530.0298730.0387370.0418350.0282190.02343

0.0434040.0375020.0209330.0249550.0302820.039050.0421510.0281640.023303

0.0433820.0375110.0208130.0249220.0303110.0393190.0423110.0282860.023679

0.0436720.037830.0210.0251860.0302770.0396980.042670.0283560.023795

0.0436690.0378730.0209570.0252980.0306720.0399920.0431650.0286090.024091

0.0436910.0379110.021070.0254590.0307150.040030.0433320.028750.024333

0.0441760.038350.0213250.0255450.0314040.0409540.0439130.0293280.024532

0.0442520.0385030.0211220.0256440.0312870.0410930.0444130.0293150.024842

0.04460.0386690.020960.0256730.03160.0413360.0444010.0294440.024716

0.0448820.0388450.0212690.0260790.0321280.0416580.0453930.0298020.025054

0.045590.0394240.0216450.0264560.0323970.0421080.0457190.030230.025543

0.0462970.0401490.0221380.026860.0327440.0426170.0463190.030570.025617

0.0472130.0411170.0226530.02740.0333940.043140.0468940.0307850.026058

0.0486720.0422220.0234540.0279010.0339820.0438470.0475110.0313430.026495

0.0506870.0437180.0245250.0289230.0347880.0448310.0484080.0319890.027131

0.0532870.0458480.0260330.0302150.0362910.0458260.0494260.0326720.027669

0.0567980.0486460.027870.0318510.0374160.0472810.050550.0334310.028196

0.0604280.0517220.0302320.033490.0391660.0486340.0518810.0344390.029032

0.0645860.0549140.0324170.0354810.0405340.0498990.0529520.0353230.02993

0.0685840.0584840.0348540.0374330.0424450.0514240.0545050.0364610.030725

0.0728040.0616980.0372910.0394220.0441080.0531180.0557530.0374120.031418

0.0772520.0653250.0398930.0415410.0457220.054790.057220.0384750.032475

0.0819660.0690510.0425970.0437180.0474670.0563660.0588280.0395860.033385

0.0872380.0731950.0457160.0461810.0497280.0581180.0605340.0407060.034557

0.0936870.0788790.0495090.0492430.0522130.0604540.062580.0425050.035727

0.1023080.0857710.0544090.0533690.0557770.0632070.0649310.0440110.037445

0.1131780.0944120.0609370.0585650.0595350.0667950.0680050.0465140.039183

0.1257530.1046560.0682960.0644370.0646790.0708710.0712440.0491640.041502

0.1374460.1140140.0750790.0695870.0688040.0743850.0742410.0513270.04335

0.1477380.1225880.081570.074770.0730680.0780310.0774080.0541320.045486

0.1577040.1303860.0871770.079420.0767040.0814240.0803040.0560980.047175

0.1692160.13970.0938430.0848230.0811090.0851540.083310.058550.049293

0.1816140.1497040.1014050.0907580.0858370.0889120.0867110.061230.051486

0.1941130.1599020.1086270.0965550.0904960.0931460.0902740.0637930.053668

0.2048160.16860.1149490.1014310.0948290.0965980.0930370.0659580.055604

0.2122880.1742410.119070.1049210.0974260.0991390.0954590.0676890.057167

0.2170930.1786240.1221680.1075150.0999920.1015290.0974270.069230.058152

0.2205420.1813210.1241750.1092140.1014570.1028760.0988470.0701140.05928

0.2239280.1843740.1260620.1108690.1032810.1047310.1004940.0712170.0602

0.2274250.187110.1279480.1123280.1047460.1063060.1018380.0723190.06101

0.2303070.1895820.1294420.1137950.106390.1083190.1040490.0734620.062084

0.2313750.1908050.1299670.1142720.1073430.1092450.1053930.0744330.062986

0.2317880.1910690.13020.1148710.1081170.110310.1070860.0750410.063697

0.2306270.1904280.1291130.1142780.1084390.1112570.1080330.0756310.064452

0.2281610.1885280.1275230.1129360.1079490.1111740.108530.0753390.064393

0.2249840.1861510.1257940.1116830.1073410.1110150.1086790.0753850.064294

0.2213490.183220.1236250.1099930.1064350.1105780.1085390.074920.06412

0.2168220.179760.1207780.1079920.1050270.1095130.107860.074520.063832

0.2126890.1766810.1183840.1061230.1039140.1088130.1076320.0739580.063432

0.2091450.1736670.1160040.104330.1028580.108520.1077070.0736540.063316

0.2059640.1715410.1141990.1031010.1023670.1083770.1079770.0735680.06331

0.2027360.1692240.1120860.101950.1017830.1082520.1085140.0733320.063133

0.1980660.1651970.1089850.0992620.1003410.1074980.1079030.0728030.062819

0.1923370.1610350.105740.096640.0989190.1068150.1079920.0726620.062849

0.188540.1579230.1030710.0950490.0979030.1061220.1076270.0720410.062534

0.1856620.1557680.1014080.0937280.0971250.1059740.1076590.0718680.062585

0.1796150.1508710.0978660.0907760.0951890.1045360.106550.0709120.061437

0.1738250.1464180.0943820.088330.0937370.103460.1061050.0702440.061263

0.1704860.143870.0921010.0868770.0927430.1033130.106340.0700480.06098

0.1678780.1422790.0905720.0858450.0925890.1032520.1065670.0699150.061262

0.1665890.1409330.0895730.085150.0923140.1036170.1070980.0699490.061376

0.1648360.139790.0884480.0845930.0922130.1037360.1076240.0701460.061488

0.1614410.1371270.0863630.082940.0915810.1037040.107840.0702950.06174

0.1570330.1335950.0835540.0808890.0906250.1031170.1079720.0698670.061497

0.1548030.1320650.0821340.0799720.0902740.1034990.1084730.0699930.061515

0.1543560.1319050.0816170.0795910.0906270.1042620.109590.0703730.061969

0.1554480.133030.0820150.0801910.0916070.1055430.1108850.0711690.062714

0.1571370.1342780.0828550.0811220.0928120.1070010.1125450.072170.063768

0.1580810.1354510.0834040.0817560.0939380.1084690.1142170.0729650.064656

0.1598780.1368150.0840350.0825080.0952820.1100270.1157880.0737850.065581

0.1638120.140330.0861010.0844570.0974710.1126530.1186280.0754390.066779

0.1694230.1453970.089260.0873160.100410.1155340.1214840.0773940.068651

0.1775890.1519620.0939440.0912020.1042690.1193390.1251530.0800050.070636

0.1870050.1600230.0993830.0958180.1086870.1237910.1294470.0827610.073393

0.1991740.1699880.10610.1015820.114040.1287280.1341650.0860060.076269

0.2142850.1825110.1149670.1087180.1206980.1350440.1401330.0900090.079658

0.2332610.1981280.1256670.117640.1285520.1422260.1467130.0946510.0837

0.257240.2180330.1398310.1291880.1386090.151480.1551760.1006390.088789

0.2868140.2420390.1569450.1431420.1508410.1625590.1650640.1079650.095035

0.3225560.2715230.177830.1600730.1654540.1756540.176730.116270.1024

0.3670470.3081270.2038040.1813150.1834280.1917940.1911930.1268120.111295

0.4223220.3535080.236260.2073890.2059170.2118350.2090970.139810.122396

0.4899590.4091040.275910.2396440.2333340.2362240.2307550.1558350.136

0.5694690.4743030.3223310.2772440.2652380.26440.2559780.1741430.151759

0.660930.549780.3768070.3209160.3019070.2969530.2850350.1954590.170124

0.7634630.6338750.437490.3699430.343220.3332420.3174090.2194580.190673

0.873120.7240560.5034350.4221270.3865730.3711440.3513820.2445160.212198

0.9849780.8156490.571630.4759910.4306490.4088390.3852930.2693270.233885

1.0930310.9056470.6388010.5285210.4728360.4449070.4172110.2934590.254529

1.1918880.9867620.6999360.5756530.5111660.4762650.4447350.3146060.272907

1.2752821.0558340.7524570.6163260.5431610.5025280.4670930.3323660.287737

Elute 1

Elute 2

Elute3

Elute 4

Elute 5

Elute 6

Elute 7

Elute 8

Elute 9

Wavelength (nm)

Absorbance

Eluted R11 with spin labeling

R11 1

650-0.003048-0.002806-0.001931-0.0022550.0012670.0005640.0035270.0045310.0084590.0036240.0097270.1204480.0946460.0697460.0559950.0443580.037890.0310.0226370.020102

649-0.00308-0.002743-0.001945-0.0021960.0012720.0005050.0035270.0046710.0084940.0036110.01006241380.12460137930.09790965520.07215103450.05792586210.04588758620.03919655170.03206896550.02341758620.0207951724

648-0.00298-0.002748-0.001908-0.0021090.0012720.0006240.003620.0046430.0084520.003583totalper plate

647-0.003104-0.002803-0.001991-0.0022330.0012310.0005650.0035190.0045840.0083750.0035980.52401620690.0131004052

646-0.00304-0.002662-0.001826-0.00210.0012630.0006340.0036580.0047040.0084770.003699

645-0.002986-0.002658-0.001877-0.0021190.0012640.0006570.0036540.0047010.0083810.003682280-6500.0369530.2331130.1915130.1316970.1125280.1058260.1047920.0995180.0650030.05846

644-0.002977-0.002626-0.001804-0.0020230.0013140.0007210.0037790.0047650.0085580.003774500-6500.0097270.1204480.0946460.0697460.0559950.0443580.037890.0310.0226370.020102

643-0.002977-0.002695-0.001955-0.0021330.0011630.0006150.003650.0047340.0084290.003655280/5003.79901305641.93538290382.02346639061.88823731832.00960800072.38572523562.76569015573.21025806452.87153774792.9081683415

642-0.003042-0.002586-0.00191-0.0020650.0012640.0006380.0037060.0047720.0084770.003766

641-0.003037-0.002622-0.001901-0.0020880.0012320.0006250.0038220.0047860.0084360.003697stdev0.6392072302

640-0.003065-0.0026-0.001869-0.0021110.0011950.0006250.0038410.0047220.0084190.003735stdev0.5031625803

639-0.002928-0.002591-0.001782-0.0020290.0013380.0007540.0039250.0048570.0084940.00373error0.2021350744

638-0.003007-0.002564-0.001838-0.002020.0012880.0007350.0039490.0047840.0084670.003773error0.1677208601

637-0.003108-0.002542-0.001893-0.0021120.0012470.000680.0038520.0047660.0083990.003718average2.5797087215

636-0.002984-0.002492-0.001778-0.0020030.0012930.0007590.0040010.0049190.0083660.003811average2.4442304621

635-0.003017-0.002538-0.001816-0.0020630.0012510.0007270.0039830.004860.0084620.003835

634-0.003027-0.002424-0.00182-0.0020260.0012750.0007270.0039890.0048610.0083510.003785

633-0.003078-0.002498-0.001821-0.0021270.0012110.0007180.0039850.0048670.0083250.003758

632-0.002968-0.00237-0.00183-0.0019860.0012750.0007820.0040960.0049510.0083950.003818

631-0.00301-0.002361-0.001762-0.0019860.0013030.0008420.0040780.0049280.008420.003869

630-0.003024-0.002375-0.001748-0.0021050.0012890.0007550.0040980.0049480.0083610.003843

629-0.002956-0.002261-0.00167-0.0019360.0013820.0009070.0042140.0049940.0084220.003907

628-0.002924-0.002275-0.001712-0.0019550.0013310.0008150.0042840.0050610.0083770.003941

627-0.002993-0.002193-0.001685-0.0019640.0013680.0008790.0042750.0050240.008420.003946

626-0.003021-0.002193-0.001607-0.0020010.0013270.0008470.0042760.0050010.0083420.0039

625-0.00292-0.00212-0.001593-0.0019280.0014280.000930.0044250.0050570.0084120.003956

624-0.002994-0.002107-0.001621-0.0018690.0013780.0009440.0043880.0051230.0083380.003915

623-0.00293-0.002006-0.001534-0.0018410.0014380.0009860.0044490.0051650.0083010.004026

622-0.002898-0.001997-0.001497-0.0018140.0014840.0009950.004510.0052120.0084090.004026

621-0.002922-0.002039-0.001534-0.0018190.0014750.0010090.0044820.0051340.0083170.003971

620-0.002931-0.00192-0.001406-0.0017180.0015580.0010410.0045150.0052790.0083310.00405

619-0.002817-0.00175-0.001305-0.0016720.0016040.0011750.0046730.0052740.008420.004083

618-0.0029-0.001805-0.001383-0.0016910.0015540.0010880.0046840.0052470.00830.004074

617-0.00289-0.001732-0.00136-0.0016720.0015860.0011340.0046790.0053030.0083140.004075

616-0.002886-0.001581-0.001273-0.0016360.0016790.0011850.004740.0053220.0083620.00408

615-0.002836-0.001581-0.001177-0.0016090.0016420.0011570.0047310.0053320.008270.00408

614-0.002841-0.001402-0.001177-0.0015120.0017120.0012360.004820.0053510.0082750.004057

613-0.002837-0.001384-0.001049-0.0015310.0017350.0012820.0048860.0053280.0082570.00416

612-0.002855-0.00126-0.000966-0.0013750.0018230.0012550.0049940.0054180.00830.004165

611-0.002769-0.001104-0.000865-0.0013930.0019250.0014070.0050130.0055250.0082820.004263

610-0.002668-0.00098-0.00075-0.0012240.0019580.00150.0051760.0055770.0082970.004273

609-0.002728-0.000847-0.000645-0.0012840.0020270.0014910.0051310.0055830.0083030.004232

608-0.002655-0.000727-0.000566-0.0011370.0020560.0014910.005220.005640.0083140.004307

607-0.002628-0.000516-0.00035-0.0010170.0021210.0016210.0053420.0057580.0083440.004336

606-0.002565-0.000373-0.000198-0.000880.0022420.0017370.0053950.0057870.0084440.004397

605-0.002589-0.000175-0.000124-0.0008390.0022750.0017750.0054950.0058030.0083720.004371

604-0.0024750.0000090.000074-0.0007510.0023870.0018720.0055290.0058930.0083550.004446

603-0.0023510.0002820.000189-0.0005630.0025270.0019650.0056280.0059930.0084890.004536

602-0.0023660.0004760.000457-0.000360.0026530.0021330.005840.0060880.0085570.004579

601-0.0022240.0006790.000568-0.0002720.0027050.0021760.0058990.0061660.0085230.004581

600-0.0022210.0009390.000767-0.000180.0028170.002260.0059580.0062340.0085930.004616

599-0.0021020.0012260.0010130.0000420.0030050.0024050.0061610.0063540.0086240.00472

598-0.0022230.0014080.0011390.0000920.0029970.0024950.0061660.0063580.008630.004681

597-0.0020910.001710.001390.0003420.0032320.002580.0063510.0064590.0086860.004763

596-0.0020510.0019670.0016510.0004210.0033270.0026790.006450.0065670.0086870.004869

595-0.0018950.0023410.0018750.0005930.0035020.0027930.00660.0067080.0087780.004971

594-0.0019430.0024920.0020490.000770.0035520.0029820.0066570.0067880.0088230.004895

593-0.0018240.0029140.0023680.001050.0038390.0031560.0068730.0068870.0089230.005053

592-0.0018210.0032620.0027150.0012040.0039270.0032250.0069870.0069540.0090540.005142

591-0.0018180.0035650.0029610.0013640.0040990.0033440.0070970.0070970.0090.005174

590-0.0017960.0039430.0032210.0016030.0043320.003530.0072490.0071880.009150.005277

589-0.0018250.0042610.0034350.0017490.0043790.0035760.0072660.0072040.009130.005278

588-0.0017940.0046550.0037430.0020080.0046130.0037660.0074710.0074090.0093370.005282

587-0.0016840.0051210.0041220.0023180.0048570.0038820.0076090.0074710.0093490.005437

586-0.0017830.0055540.0045160.0025360.005040.0041340.0077810.0076240.0094610.005512

585-0.0016630.0061020.0048350.0028340.0053030.0043160.0079670.0077820.0095440.005582

584-0.0016970.00660.0051990.0031090.0054540.0044520.0080260.007850.0095610.005686

583-0.0016890.0070030.0056370.0033880.0057080.0047330.0082730.0080110.0096670.005807

582-0.0017510.0075030.0059690.0036320.0059160.004780.0083470.0081270.0096990.005741

581-0.0015790.0082060.0065540.0040390.0063170.0050220.0085970.0082820.0099790.005999

580-0.0015940.0088260.0070610.0043620.0064860.0053330.0088160.0084720.0100940.006092

579-0.0015060.0094990.0075070.0048040.0067970.0055340.0089770.0086040.0102080.006137

578-0.0014880.0101410.0080450.0051140.0071290.0057690.0091920.0088380.0103080.006306

577-0.0013940.0109020.0086340.0056280.0075260.0059460.0094330.0090260.0105170.006369

576-0.0013620.0116980.0092480.0060420.0078520.0063230.0096650.0092150.0106440.006551

575-0.0012410.0125390.0099510.0065340.0082130.0066050.0098590.0094330.0107380.006677

574-0.0012930.0132910.0104430.0069590.0086070.0068640.010150.0095690.0109230.006826

573-0.0013120.0141220.011080.0072990.0089050.0071660.0103390.0096770.0109740.006856

572-0.0011620.0150670.0119310.0080240.0094310.0075420.0106360.0100360.0111990.007074

571-0.0011010.0161170.012660.0085550.0098490.0079340.0109490.0103140.0114640.007275

570-0.0009980.0171430.0135220.0091110.0102580.0082740.0113020.0105170.011610.007419

569-0.0010310.0181480.0142870.0096860.0107960.0085990.0115750.0107380.0117060.007509

568-0.0009040.0192270.0151760.0102720.0112920.0089530.0118510.0110180.0119710.007691

567-0.0008250.0203330.0160280.0109890.0118370.0094330.0121840.0112930.0121750.007844

566-0.0006840.0216390.0170730.0117770.0124330.0098630.0125730.0115460.0124620.008053

565-0.0005530.0228080.0180240.0125240.0130030.0103290.0129930.0118780.0126310.008364

564-0.0005670.0241220.0189460.0131440.0135260.0107860.0133180.0121780.0129120.008493

563-0.0003140.0254550.0200520.0140040.0141390.0112420.0137040.0125920.0132250.008818

562-0.0003610.0267450.0210810.0147460.0147410.0117810.0141350.012790.0134090.008875

561-0.0002950.0282630.0222180.0155950.0155030.0121950.0144880.01320.0136790.00909

560-0.0001880.0296880.0233660.0163010.0160970.0127460.0148620.0133940.0139170.009297

5590.0000230.0312960.0245990.0172620.0168380.0132650.0154170.0138410.0142380.009582

5580.0001550.0328470.0258970.0182490.0175690.0138760.0158470.0143220.0144960.009801

5570.0002820.0345320.027220.0192130.0185070.0145670.0165230.0147710.0149360.010192

5560.0003710.0362330.0285560.0201760.0191790.0151790.016850.0150580.0152130.01037

5550.0004880.0379210.0298730.0211190.0199480.0157610.017390.0154790.0155130.010662

5540.0006620.0396440.0312320.0221850.0208050.0164010.0179050.0159830.0159340.010987

5530.0007230.0413070.0325290.0231470.0215810.0169980.0183430.0162820.0161010.011263

5520.0008780.0430840.0340030.0242340.0224020.017640.0189270.0167760.0165370.011463

5510.0010990.0449330.0354880.0252290.0232790.0182590.019440.0171840.016930.011814

5500.0011790.0468420.0368190.0263790.0241270.0189550.0200240.0175890.0172380.012084

5490.001240.048680.0383810.0274750.0250470.0196590.0205880.0180960.0176460.01243

5480.0014430.0505980.0398120.0285650.0258380.0203660.0210940.0185550.0180030.0127

5470.0016120.0525790.0413990.029640.0267620.0210670.0216630.0190530.0183090.012979

5460.0016780.0544650.0428740.0307480.0276620.0217190.0221230.0194410.018760.01326

5450.0019370.0564670.0444930.0319510.0286170.0224290.02280.0200060.0191480.013639

5440.002130.0584360.0460660.0331610.0295670.0231390.023470.0204470.0194840.014045

5430.0022290.0604270.0475550.0342660.0304920.0239260.0240060.0209680.0198820.014248

5420.0023940.0625060.049190.0354340.0314030.0245810.0245810.0213720.0202010.014602

5410.0024830.0644010.0507630.0366120.0322910.0253160.0251920.0219340.0206390.014966

5400.0026720.0664750.0523010.0377710.0332610.0260650.0257660.0223950.0209710.015303

5390.0028610.0685730.0540510.0390020.0342930.0268110.0264170.0229370.0213970.015598

5380.0029130.0705470.0555370.040070.0352060.0275380.0269680.023350.0217550.015911

5370.0031920.0725070.0571460.0412410.0361240.0282540.0275640.0238610.0222730.016233

5360.0033010.0744210.0587180.0423710.0370260.0289840.0281420.0243550.0225820.016537

5350.00340.076440.0601680.0435340.0379020.0296880.028730.0248480.0229540.016859

5340.0035660.0784430.0618060.0447460.0389660.030420.029350.0253480.0234080.017259

5330.0037930.0805070.0633580.0458490.0398640.0311440.0299260.025850.0238040.017629

5320.0038920.0823430.0649470.0469970.0407430.0318740.0304830.0263010.0241030.01789

5310.0039970.0843250.0663240.04820.0416140.0325640.031040.0267420.0244780.018126

5300.004210.0862330.0680190.0492810.0425910.0333090.0316970.0273130.0249770.018575

5290.0043380.0880990.0694270.0503490.0433870.0339310.0322450.0276460.0253120.018811

5280.0045080.0899530.0708550.0514140.0442540.0346010.0327910.0281510.025640.01914

5270.0045610.0916260.0721870.0524580.0451290.0352820.0332820.0285860.0259230.019368

5260.0047560.0934460.0734940.0534630.0458670.0358920.0337770.0290760.0263150.019691

5250.0048840.0950760.0748040.0544240.0467120.0364660.0342620.02950.0266520.020012

5240.0048670.0964850.0760250.055210.0473550.0369390.0347360.0297360.0268510.020206

5230.0050660.0981840.0773440.0561650.0481210.0375890.0352920.0302510.0272620.020484

5220.0051990.0997070.0785350.0570760.0488460.0381680.0356130.0306230.0274510.020659

5210.0053560.1011870.0797420.0580110.049470.0387610.0361470.031050.027850.021037

5200.0054850.1026670.0808260.0587010.0501230.0392850.0365540.0313660.0280990.021183

5190.0055280.1039960.0819340.059520.050830.0397310.0369210.0316380.0282920.021412

5180.0056520.1052620.0829270.0602960.0513960.0402410.0373560.0320570.0286430.021678

5170.0056620.1064780.083830.0610110.0519640.0406450.0376440.0322660.0288650.021748

5160.0057660.107610.084750.06170.0525420.0410590.0380910.0326360.0290520.022055

5150.0059940.1088810.0857540.0623960.0531730.0415720.0385390.0329890.0293570.022278

5140.0060130.1097370.0865180.0629010.0536080.0418750.03880.0331790.0295810.022394

5130.0060520.1107820.0871370.0634660.0539310.0421520.0390430.0333930.0296920.022536

5120.0062080.1117590.0881010.0641120.0545430.0426510.0393950.0337460.0300430.022814

5110.0061630.1124220.0886520.06450.0547330.0428110.039620.0338040.0300130.022871

5100.0062950.1132960.0892970.0650640.0551950.0431530.0398670.034150.0302610.023012

5090.0062910.1139990.0899830.0653550.0555960.0434850.0400830.0343320.0303550.023113

5080.0064390.1147170.0904490.0658340.0558720.0437590.040350.0345040.0305960.023306

5070.0064820.1153210.0908560.0661950.0562080.0440120.0405130.0347560.0306290.023486

5060.0065220.1157910.0913260.0664370.0563790.0442070.0407160.0348740.0307150.023471

5050.0065850.1162770.0915490.0667490.0565370.044250.0409080.0350520.0308360.023563

5040.0065990.1167340.0919970.0669950.0568360.0444350.0410960.0352130.0309490.023644

5030.0066620.1170980.0923040.0671680.0569780.044750.0411920.0353520.030960.023745

5020.006720.1173060.0924870.0673280.0571440.0447590.041350.0354570.0310590.023831

5010.0066490.1175170.0927050.0674030.0571620.0448730.041380.0354750.0310960.023791

5000.0066790.1176420.0927150.0674910.0572620.0449220.0414170.0355310.0310960.023726

4990.0068260.1178410.0928660.0675930.0573320.0450020.0415750.0357090.0312460.02391

4980.0069650.1178560.0928610.0676260.0573790.0450830.0416350.0357310.0312590.024046

4970.0068940.1177320.0928340.067520.0572530.0450220.0417180.0357210.0312430.023979

4960.0068320.1176150.0927620.0674390.0572420.0450210.0416760.0357810.0311650.023882

4950.006990.1174260.0925790.0673380.0571330.0450660.041690.0358560.0311490.023877

4940.0069630.1170650.0924170.0671670.0571510.0449130.0416150.035750.0310980.023864

4930.0069720.1168990.092280.067080.0570780.044870.0416090.035790.0310780.023955

4920.0070020.11650.0919790.0667190.056830.0448030.0415620.0357230.031010.023856

4910.0069560.1160250.0915190.0664660.0566820.04460.0414210.0356850.0309460.023797

4900.0069080.1156180.0913090.0662680.0564190.0446880.0414210.0356790.0309150.023822

4890.00690.1150350.0907630.0659480.0561950.0444350.0413140.035450.0307480.02361

4880.0068870.1144870.0903630.0655840.0559080.0442430.041190.035520.030650.023593

4870.0068930.1137930.0898290.0651460.0555090.043930.0409720.0354570.0305110.023529

4860.0068740.1131990.0894190.0648630.0553560.0438440.0409690.0353940.030590.023488

4850.0068890.1124070.0887460.064420.0550390.0435340.0407790.0353020.0304230.023412

4840.0067990.1116410.0881910.0639530.0546390.0433410.0405510.0351530.0303310.02338

4830.0067520.1107640.0874950.0635040.0542090.0429520.0404120.0349940.0301180.023239

4820.00670.1099270.086810.0629630.0538550.0427510.0402230.0348590.0300230.023185

4810.0068010.1090140.0860120.062380.0534060.0424890.0399880.0347640.0298790.023048

4800.0068870.1080770.0853970.0618240.0529780.0421750.0398260.0346230.0296790.023009

4790.0067980.1070510.0845150.0612370.0525390.041750.0394740.0343820.029570.022757

4780.006760.1060490.0837730.060630.051890.041370.0391730.0340690.029250.022515

4770.0066030.1049420.0828850.0599750.0514810.0410210.0389450.0338930.0291270.022463

4760.0064940.1038390.0820620.0593540.0509720.0406530.0386290.0337490.0289030.022296

4750.0065420.1026520.081160.0586810.0505230.0403590.038440.0335360.0287980.022118

4740.0065130.1014860.0802920.0579850.0499180.0399120.0381390.0333910.0285890.022041

4730.0063940.1002250.0792250.0573020.0493840.0395180.0378910.0331450.0283050.021884

4720.0063750.0990250.0783690.0565780.0488430.0391530.0376040.0329320.028170.021766

4710.0062750.0978080.077370.0557930.0482080.0387020.0373030.0327410.0279350.021495

4700.0061950.096470.0762760.0550390.0475970.0382220.0368910.0324650.0277120.021363

4690.0062230.0951030.0753380.0542810.0471360.0379310.0365910.0322750.0275190.021248

4680.0061370.0939170.0743650.0535170.0464420.0374770.0363270.0320190.0272250.021101

4670.0061030.0925740.0733360.0528290.0459070.0370780.0359960.0318140.0271240.020923

4660.0059750.0911780.0721920.0519030.045110.0365110.035660.031440.0267930.020646

4650.0059690.0897560.0711820.051180.0445090.0361470.0353340.0313040.0266040.020539

4640.0058030.0883680.0700550.050250.0438780.0355890.0349660.0310550.0263120.020305

4630.0057920.0871320.0690050.0495440.0433720.0351440.0346750.0308030.0260830.020154

4620.0058540.0857320.067950.0487590.042660.0347990.0343090.0306680.0259460.020048

4610.0056690.0843550.066960.0478820.0420750.0343050.0339280.030350.025770.019795

4600.0056060.0830010.0659070.0472540.0414290.0338750.0337070.0300750.0254590.019666

4590.0056170.081680.0648320.0463580.0407620.033360.0332530.0298620.0250770.019397

4580.005540.0802680.0637640.0456120.0401810.0329980.0329520.0296190.0249570.019357

4570.0054220.0789240.0626890.0447280.0395290.0324870.0325780.0294340.0247130.018997

4560.0054690.0775490.0616860.0440240.0389160.0320620.0324070.0291280.0244210.018936

4550.0053510.0761270.0606480.04320.0382390.0316070.0320080.0288630.0242270.018704

4540.0053560.0748970.0595680.0424040.0377610.0311550.0316930.0287050.0240310.018588

4530.0052810.0736190.0585620.0417190.0371220.0307880.0314010.0285110.0237880.018386

4520.0052870.0722280.0575320.0408530.0364910.0303560.0311560.0282770.023610.018199

4510.0052180.0709720.0564080.0401840.0359030.0298090.0307560.0280280.0232720.017961

4500.0052060.0696210.0555180.0393860.0353360.029540.0304210.0278510.0231440.017822

4490.0052320.0684290.0546320.0386460.0348060.0291770.0302650.0276890.0229580.01773

4480.0051060.0670330.0535390.0379330.0341840.0286560.029980.0275310.0227180.017619

4470.0049050.0655740.0523520.0370050.0335370.0280970.0295580.0270790.0224660.01733

4460.0049750.0644140.0514380.0362690.0329730.0277390.0293320.0270030.0223140.017266

4450.0048210.0629960.0503550.0355090.0322260.0273390.0288960.0266690.0220140.01699

4440.0047520.0617780.0493970.0348210.0317710.0269520.0286050.026610.0219630.016882

4430.0047030.0604080.0483860.0340060.0311530.0264080.0283380.0263680.0216550.016752

4420.004720.0591670.0474460.0332530.0305760.0260670.0280620.0261520.0214290.016589

4410.0046730.058230.0466540.0326620.0301810.0257350.0279020.0260280.0214840.016588

4400.0045240.0567390.0455770.0318980.0294810.025270.0274820.0257940.0211290.016203

4390.0044680.0557460.0449320.0311860.0289750.0250030.0271640.0255780.0210280.016126

4380.0044450.0545890.0440780.0305990.0284760.0245640.0269610.025390.0207860.016068

4370.0043610.0534060.0430380.0299290.0281180.0242870.0267750.0253350.0205840.015914

4360.0043050.0522760.0423130.0293590.0276870.0240140.0265470.0252180.0204020.015871

4350.0041820.051150.0414350.0288370.0270390.0236870.026350.0250570.0204050.015877

4340.004280.0501810.0406030.0280780.0266630.0232770.0261210.0248020.0202410.015579

4330.0042010.0489420.0398030.0273360.0261090.0228710.0257650.0246740.0199570.015548

4320.0043260.0481360.0391910.0268870.0256860.0226570.02560.0246160.0199640.015455

4310.004140.0470750.0382820.0262560.0252390.0223770.0254260.0244310.0197190.015255

4300.0040930.0461310.0376320.0257480.0248990.0221290.0250860.0244340.0197220.015232

4290.0040080.0452210.0368750.0251790.0242490.0216180.0248650.0241030.0194570.015027

4280.0040120.0444150.0361690.0247460.0239780.021550.0248620.0242050.0193540.015129

4270.0039210.0434510.0356050.0241740.0235390.0211020.0245680.0239720.0192470.014884

4260.0038990.0426650.0348460.0237180.0230730.0208240.0243440.0237740.0190440.014692

4250.0040330.0418790.03440.023350.0228510.0207980.0242320.0237930.0190890.014731

4240.0039440.0410170.0337710.0228020.0223140.0203020.0241020.0237490.0188830.014678

4230.0038290.0402630.0332290.0223070.0221010.0201450.0239290.0236110.0188110.014641

4220.003720.0394810.0326530.0219610.0216590.019910.0237190.0234010.0187010.014495

4210.0038590.0389040.0322240.0215930.0214820.0198040.0235910.02350.0186250.01441

4200.0037340.0381890.0316680.0211590.0210440.0195410.0234690.0233580.0184780.014314

4190.0038120.037460.0312940.0208640.0207990.0193570.0233480.0233230.0184290.014319

4180.0038410.0369260.0308380.0204870.0205920.0192210.0233070.0233230.0184370.014287

4170.0038080.0363450.0304170.02020.02030.0190140.0231750.0232660.0183460.01425

4160.0037610.0357740.0300380.0198780.0200930.0188780.0230530.0232650.0182240.014149

4150.0037320.0351440.0296140.0195570.0197970.0186870.0229880.0231740.0181280.014097

4140.0038490.0347250.0293270.0193050.01960.0186450.0229260.0232190.0181810.01411

4130.0037770.034240.0290210.0190490.0194590.0184940.0228450.0232180.018140.014132

4120.0038120.0338620.0287340.0188270.0191870.0183770.0228380.0231920.0181080.014055

4110.0037790.03340.0284710.0185970.0190020.0182570.0228290.0232230.0180370.014024

4100.0038520.0330660.0282360.0183910.01890.0181660.0227580.0232070.0180460.014121

4090.003920.032710.0280160.0182340.0187830.0181590.0227510.0232870.0180690.014044

4080.0038820.0324330.0278650.0180450.018630.0181350.0227130.0233030.018150.01409

4070.0038350.0320340.0276310.0178580.0184420.0179980.0227930.0232830.0180220.013937

4060.003880.0318560.0274640.0177020.0183570.0178920.0226920.0233950.0180720.014064

4050.0038750.0316250.0273580.0176630.0182720.0180120.0228440.0235110.0180620.014074

4040.0038860.0313610.0272480.0174580.0181530.0178480.022730.0234730.0180930.014088

4030.0040650.0312670.0272060.0174550.0181850.0179250.0228490.0236430.018210.014125

4020.0040270.0310720.027160.0173740.0180430.0179430.0228780.0237230.0182230.014236

4010.0041010.0309910.0270440.0173080.0180520.0178870.0229140.0238150.0181870.014264

4000.0041210.0309050.0269880.0171970.0180210.0180160.0229440.0239310.0182910.014292

3990.0041130.0307630.0269780.0171390.0179080.0179330.0230490.0239760.0182480.014268

3980.0041230.0307350.026940.0170930.0179630.0179780.0231110.0240590.0183880.014316

3970.0042020.0306420.0269440.0170560.0178950.017980.0231650.0241640.0182760.014368

3960.0042470.0305910.0269480.0170620.0178310.017