Revlon EISB Performance Status Update – February 2016

OBG PRESENTS:

EVO / Bioaugmentation for Treatment of Trichloroethene by Biobarrier and Source Injection ApproachChristine Fogas (OBG), Michael Kozar, P.G. (OBG), Bella Bakrania (OBG), and Eric Schleicher, P.G. (OBG)

Fourth International Symposium on Bioremediation and Sustainable Environmental TechnologiesMay 22-25, 2017, Miami, FL

http://www.obg.com/

Emulsified Vegetable Oil (EVO) and Bioaugmentation for Treatment of Trichloroethene(TCE) by “Biobarrier” and Source Injection Approach

2

Background

Implementation

Performance Monitoring

3

Background

Site Setting

4

6

Remedial Objectives

Remediate source / reduce CVOC mass flux

Enhance monitored natural attenuation (MNA) downgradient

Meet New Jersey Groundwater Quality Standards (GWQS) – TCE = 1 ppb; 1,1,1-TCA = 30 ppb

Key Considerations

Difficult urban setting

Treatment needed off-Site

Long-term solution / low maintenance

No aboveground equipment

7

▪ Showed TCE degradation, but accumulation of cDCE with electron donor only (MEAL)

▪ Showed complete dechlorination to ethene with Dhc addition (KB-1)

2001 Microcosm

▪ Conducted at a “hot-spot” in shallow source area

▪ 1st Injection = 65 lbs sodium lactate; 2nd Injection = 600 lbs sodium lactate

▪ Bioaugmentation with Dhc

2003 Field Pilot Study

▪ Used groundwater from off-site monitoring well where “biobarrier” was planned

▪ Tested new commercially available electron donors and buffers

2010 Microcosm

Enhanced In Situ Bioremediation (EISB) Proof of Concept

8

Cross Sectional View of Site

Biobarrier Segment “B”

Biobarrier Segment “A”

Source Area

9

Implementation

10

Overview / Chronology

11

• Initial (8) injection wells for EISB pilot test in source area installed June 2003

• Additional (25) injection wells installed January – March 2014

• Newman Zone® Standard Emulsified Vegetable Oil (EVO) was selected

• EVO injection completed over a period of 14 days (September 2014 – October 2014)

• Bioaugmentation conducted May 2015

• TSI-DC Dehalococcoides mccartyii Bioaugmentation Culture® supplied by Terra Systems, Inc.

• Groundwater (GW) sampling / monitoring events conducted:•Baseline (pre-EVO injection): May 2014

•Months 1 through 6: November 2014 – April 2015 (pre-bioaugmentation)

•Months 7 and beyond: August 2015 – April 2017 (post-bioaugmentation)

Injection Well

Install

EVO Injection

Bioaugment


Injection WellInstallation

12

Off-site barrier well installations

9-ft high pile of plow snow covering two

planned drill locations

Compact Rotosonicdrill rig

EVO Injections

13

Newman Zone® product tote

Spill containment Gravity feed setup

Mixing tanks

Biobarrier injections

14

Actual vs. Target EVO Injected in Pounds

A total of 25,937 lbs of Newman Zone® Standard EVO at 10% concentration (31,438 gallons in solution) was injected in the EISB treatment zones over a period of 14 days

3,310

9,981

12,646

0

2,000

4,000

6,000

8,000

10,000

12,000

14,000

EVO

Inje

cte

d (

lbs)

SOURCE AREA BARRIER SEGMENT A BARRIER SEGMENT B

StoichiometricTarget LOW

(1,620)

Injection Porosity Target HIGH

(11,520)


(11,520)

Stoichiometric Target LOW

(3,266)


(12,370)

Stoichiometric Target LOW

(1,814)

Bioaugmentation

15

TSI-DC Bioaugmentation Culture® contains > 1 x 1011 cells/L of Dehalococcoides mccartyii

Self-performed bioaugmentation

Fouling Slowed Progress

Total of 336 liters of culture injected over a period of 7 days to achieve target concentration of 1 x 107 cells/L in groundwater

16


EVO is fermented to release volatile fatty acids (VFAs), which act as a carbon source for Dhc and other microbes

17

EVO InjectionsComplete

Bioaugmentation

0

200

400

600

800

1000

1200

1400

0

100

200

300

400

500

600

Co

nce

ntr

atio

n (

mg

/L)

MW-8C: Volatile Fatty Acids (VFAs) (Source Area - Shallow GW Zone)

Lactic Acid

Acetic Acid

Propionic Acid

Formic Acid

Butyric Acid

Pyruvic Acid

EISB Monitoring Wells

18

19

Baseline TCE = 3,320 ppb

April 2017 TCE = 18.4 ppb

NJ GWQS for TCE = 1 ppb


Bioaugmentation

0

100

200

300

400

500

600

700

800

900

1,000

0

5

10

15

20

25

30

35

40

45

50

May

-14

Jun

-14

Jul-

14

Au

g-1

4

Sep

-14

Oct

-14

No

v-14

Dec

-14

Jan

-15

Feb

-15

Mar

-15

Ap

r-1

5

May

-15

Jun

-15

Jul-

15

Au

g-1

5

Sep

-15

Oct

-15

No

v-15

Dec

-15

Jan

-16

Feb

-16

Mar

-16

Ap

r-1

6

May

-16

Jun

-16

Jul-

16

Au

g-1

6

Sep

-16

Oct

-16

No

v-16

Dec

-16

Jan

-17

Feb

-17

Mar

-17

Ap

r-1

7

May

-17

Tota

l VFA

Co

nce

ntr

atio

n (

mg

/L)

VO

C C

on

cen

trat

ion

(µ

M)

MW-8C: EISB Source Area - Shallow Overburden Zone

PCE TCE cDCE VC Ethene + Ethane Total VFA's

20



April 2017 TCE = < 0.26 ppb

0

150

300

450

600

750

900

1,050

1,200

1,350

1,500

0

10

20

30

40

50

60

70

80

90

100

May

-14

Jun

-14

Jul-

14

Au

g-1

4

Sep

-14

Oct

-14

No

v-14

Dec

-14

Jan

-15

Feb

-15

Mar

-15

Ap

r-1

5

May

-15

Jun

-15

Jul-

15

Au

g-1

5

Sep

-15

Oct

-15

No

v-15

Dec

-15

Jan

-16

Feb

-16

Mar

-16

Ap

r-1

6

May

-16

Jun

-16

Jul-

16

Au

g-1

6

Sep

-16

Oct

-16

No

v-16

Dec

-16

Jan

-17

Feb

-17

Mar

-17

Ap

r-1

7

May

-17

Tota

l VFA

Co

nce

ntr

atio

n (

mg

/L)

VO

C C

on

cen

trat

ion

(µ

M)

MW-21: EISB Biobarrier - Deep Till Zone


21





Bioaugmentation

0

150

300

450

600

750

900

1,050

1,200

1,350

1,500

0

5

10

15

20

25

30

35

40

45

50

May

-14

Jun

-14

Jul-

14

Au

g-1

4

Sep

-14

Oct

-14

No

v-14

Dec

-14

Jan

-15

Feb

-15

Mar

-15

Ap

r-1

5

May

-15

Jun

-15

Jul-

15

Au

g-1

5

Sep

-15

Oct

-15

No

v-15

Dec

-15

Jan

-16

Feb

-16

Mar

-16

Ap

r-1

6

May

-16

Jun

-16

Jul-

16

Au

g-1

6

Sep

-16

Oct

-16

No

v-16

Dec

-16

Jan

-17

Feb

-17

Mar

-17

Ap

r-1

7

May

-17

Tota

l VFA

Co

nce

ntr

atio

n (

mg

/L)

VO

C C

on

cen

trat

ion

(µ

M)

MW-22: EISB Biobarrier - Deep Till Zone


22

TCE Concentrations (ppb)Historic Range* April 2017 Results

Source Area Monitoring Wells

MW-8 (shallow overburden)+ 110 – 3,300 < 0.26

MW-8B (shallow overburden) 54 - 760 5,270

MW-8C (shallow overburden) 130 - 6,100 18.4

MW-8D (shallow overburden) 42 - 17,000 < 0.26

MW-20 (intermediate till) 400 - 25,000 580

MW-18 (deep till) 130 - 340 148

Barrier Segment “B” Monitoring Well

MW-21 (deep till) 5,500 - 16,000 < 0.26

Barrier Segment “A” Monitoring Well

MW-22 (deep till) 580 - 9,000 2.1Notes:NJ GWQS for TCE = 1 ppb, cDCE = 70 ppbJ = estimated value* Excludes data from the EISB Pilot Test period (July 2003 – April 2004)+ cis-DCE concentrations shown for MW-8 (pilot test well)

23

1,1,1-TCA Concentrations (ppb)

Historic Range* April 2017 Results

Source Area Monitoring Wells

MW-8 (shallow overburden) 25 – 1,000 0.72 J

MW-8B (shallow overburden) < 1 - 24 714

MW-8C (shallow overburden) 1.2 - 190 0.78 J

MW-8D (shallow overburden) 2.5 - 710 < 0.22

MW-20 (intermediate till) 2.7 – 1,100 25.6

MW-18 (deep till) 0.7 – 5.9 0.42 J

Barrier Segment “B” Monitoring Well

MW-21 (deep till) < 13 - 100 < 0.22

Barrier Segment “A” Monitoring Well

MW-22 (deep till) 3.6 - 520 < 0.22

Notes:NJ GWQS for TCA = 1 ppbJ = estimated value*Excludes data from the EISB Pilot Test period (July 2003 – April 2004)

Sentinel Monitoring Well

24

25


April 2017 TCE = 2,670 ppb


Bioaugmentation

5/1/20145/31/20146/30/20147/30/20148/29/20149/28/201410/28/201411/27/201412/27/20141/26/20152/25/20153/27/20154/26/20155/26/20156/25/20157/25/20158/24/20159/23/201510/23/201511/22/201512/22/20151/21/20162/20/20163/21/20164/20/20165/20/20166/19/20167/19/20168/18/20169/17/201610/17/201611/16/201612/16/20161/15/20172/14/20173/16/20174/15/2017

0

150

0

5

10

15

20

25

30

35

40

45

50

May

-14

May

-14

Jun

-14

Jul-

14

Au

g-1

4

Sep

-14

Oct

-14

No

v-14

Dec

-14

Jan

-15

Feb

-15

Mar

-15

Ap

r-1

5

May

-15

Jun

-15

Jul-

15

Au

g-1

5

Sep

-15

Oct

-15

No

v-15

Dec

-15

Jan

-16

Feb

-16

Mar

-16

Ap

r-1

6

May

-16

Jun

-16

Jul-

16

Au

g-1

6

Sep

-16

Oct

-16

No

v-16

Dec

-16

Jan

-17

Feb

-17

Mar

-17

Ap

r-1

7

Tota

l VFA

Co

nce

ntr

atio

n (

mg/

L)

VO

C C

on

cen

trat

ion

(µ

M)

MW-29: Sentinel A - Deep Till Zone


26

Post-Bioaugmentation

Sample collected for CENSUS-DNA analysis by Microbial Insights, Inc.

June 2015 Jan 2016 April 2017

MW-8C 1.90 x 109 N/A N/A

MW-20 2.13 x 107 N/A 3.46 x 10

6

MW-21 2.84 x 104

3.28 x 108

N/A

MW-22 7.58 x 108 N/A N/A

MW-29 4.80 x 103 N/A 1.90 x 10

3

MW-25BR N/A 2.20 x 108 N/A

Note:

Literature established 1 x 107 cells/L as a threshold for

generally useful rates of dechlorination (Lu et. Al., 2006)

CENSUS-DNA:Dehalococcoides (Dhc) Results (cells/L)

27

January 2012TCE = 850 ppb


EVOInjectionsComplete

Bioaugmentation

0

100

200

300

400

500

600

700

0

2

4

6

8

10

12

Jan

-12

Mar

-12

Jun

-12

Sep

-12

Dec

-12

Mar

-13

Jun

-13

Sep

-13

Dec

-13

Mar

-14

Jun

-14

Sep

-14

Dec

-14

Mar

-15

Jun

-15

Sep

-15

Dec

-15

Mar

-16

Jun

-16

Sep

-16

Dec

-16

Mar

-17

Jun

-17

Be

nze

ne

Co

nce

ntr

atio

n (

μg/

L)

VO

C C

on

cen

trat

ion

(µ

M)

MW-25BR: Sentinel B - Bedrock ZoneChlorinated Ethenes / Ethene + Ethane Concentrations

vs. Benzene Concentration over Time

Ethene + Ethane not measured

Baseline GW Sampling Event

(Pre- Full-Scale EISB)

Methane production is expected as a result of EISB

28

MW-8C MW-20 MW-21

Shallow/Source Int/Source Deep/Biobarrier

5/28/2014 8.4 2.9 230

11/4/2014 12 19 35

1/5/2015 280 20 3,300

6/10/2015 1,900 120 13,000

8/26/2015 3,300 440 27,000

1/14/2016 9,700 -- 26,000

9/26/2016 11,000 11,000 27,000

4/21/2017 15,000 6,300 17,000

Dissolved Methane in GW Results (ug/L)

Multigas meter used to check:

▪ Interior of Site building

▪ Basement spaces across street

▪ Nearby drainage inlets

▪ Shallow monitoring wells downgradient

No evidence of methane accumulation was found

Injection wells fitted with vented well caps to prevent methane gas buildup

Performance Monitoring Conclusions

29

EVO + bioaugmentation injections were successful in establishing conditions for EISB in the source area and biobarrier treatment zones

VFA depletion is not an immediate concern along the biobarriers and majority of source area

Dhc population is thriving in EISB treatment zones, as well as in the downgradient bedrock zone

Some of the VFAs are being utilized by methanogens, resulting in high levels of dissolved methane in groundwater

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Revlon EISB Performance Status Update – February 2016

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