SLIDES

Schemes for slides

With background Without background

Gram positive cocci Gram positive bacilli Gram negative cocci

Scheme for slides

With background Without background

Brown Blue Red

Organism Gram +ve cocci Organism Gram -ve

Staphylococci in culture

Staphylococci in culture

Streptococci in culture

Streptococci in culture

Candida

Neisseria in culture

Corynebacterium diphtheriae

Clostridium tetani

Clostridium tetani

Staphylococci in pus

Staphylococci in Pus

Streptococci in Pus

Streptococci in pus

Vincent’s angina

Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)

Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)

Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)

Spirochaetes(Fontana stain)

Spirochaetes (fontana stain)

Spirochaetes (fontana stain)

MEDIA

Nutrient agar

Staphylococcus aureus on nutrient agar( showing golden yellow endopigment )

Blood agar

Types of hemolysis on Blood agar

Alpha-hemolysis on blood agar

Beta-hemolysis on blood agar

Non-hemolysis on blood agar

Chocolate agarfor Neisseria

Loffler’s serumfor C. diphtheriae

Lowenstein Jensen mediumfor M. tuberculosis

Cooked meat medium for anaerobic cultivation

TESTS

Coagulase Test

Staphylococcus aureus

Coagulase negative staphylococci, e.g. S. epidermidis

Coagulase test

Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis

Antibiotic Sensitivity Test (Disc diffusion)

Sensitive to: 1, 2, 3, 4, 6

Resistant to:5

1

5

62

4

3

Antibiotic Sensitivity Test (Disc diffusion)

Sensitive to: 1, 2, 5, 6

Resistant to:3, 4

1

5

62

4

3

Phage typing

1 2

3 4 5 6

7 8 9 10

11 12 13 14

15 16 17 18

19 20

Sensitive to 4, 5, 6,……..etc.

SEROLOGY

Elek’s Test

Toxigenic C. diphtheriae Non-toxigenic C. diphtheriae

Simple tube agglutination test(Titre:1/320)

TITRE

1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control