R.Ravichandran Email: ravimibt@gmail.com Phone: +91-9843700367

Objective

To refine and rejuvenate my intellectual competency by pursuing active research works

in my area of interest.

Educational qualifications

PhD June 2010- till date

Centre for Bioseparation technology (CBST)

VIT University, Vellore

Tamil Nadu.

Thesis title: Development and characterization of murine monoclonal antibodies against B.abortus porin

(Outer membrane protein) for rapid immunodiagnosis of brucellosis in human and bovine

(Submitted for thesis evaluation in March 2015)

Supervisor: Dr.N.S.Jayaprakash

Master’s Degree M.Tech in Industrial Biotechnology (5 year Integ.)

April 2005 to May 2010

SASTRA University, Thanjavur, Tamil Nadu.

Technical skills I am proficient in the following techniques listed which I have been doing regularly as part of the

research projects undertaken:

BIACORE 3000 (Membrane protein characterization)

o Monoclonal antibody-membrane protein affinity measurement

o Handled various biosensor chips (NTA , CM5, HPA)

Molecular biology

o Cloning, expression and purification of bacterial membrane proteins

o Handled different bacterial vector system (pET 28a, pET 20b)

Liquid Chromatography systems

o HPLC : Waters - analytical ; FPLC : AKTA (Amersham Biosciences)

Chromatographic techniques

o Mixed mode chromatography ; Affinity Chromatography (Protein A, Protein

G); Size exclusion, IEX, HIC, IMAC, RP-HPLC

(I have handled all these chromatographic techniques for both the purification of membrane

proteins, monoclonal antibodies as well as fractionation of DNA, viruses)

Other techniques and skills

SDS-PAGE (1D and 2D),Western Blotting ,Protein quantification, ELISA, Basic

microbiology, Mammalian cell culture.

Good communication and leadership skills.

Ability to take up projects as well as work in a team.

Good in soft skills like Microsoft word, excel, power point, Graph pad prism etc.

Research experience 4 years 10 months

Centre for Bioseparation Technology (CBST), VIT University, India

Ph.D Research work: Rapid immunodiagnosis of brucellosis in human and bovine (ICMR-ADHOC, Gov.of.India.)

Responsibilities:

Developed a sandwich ELISA assay for the detection of Brucella outer membrane protein

(porin) in human and veterinary sera samples using highly specific and sensitive

monoclonal antibodies for the diagnosis of brucellosis.

Developed an indirect ELISA for the detection of specific antibodies to recombinant

Brucella Outer membrane porin. The work was carried out in collaboration between

(CBST), VIT University, Chistian Medical College, Vellore and Department of

Veterinary public health and preventive medicine, TANUVAS, Chennai.

Other projects Involved

S.NO

Projects

Outcome/achievement

1.

Developed Specific monoclonal antibodies

against HRP II (P.falciparum)

A rapid lateral flow test for

Malaria diagnosis

2. Developed Specific monoclonal antibodies

against surface lipid antigens of M.bovis

A rapid liposomal agglutination

test for Tuberculosis diagnosis

3. Developed Specific monoclonal antibodies

against a fungal lectin (Sclerotium rolfsii)

Developed mAb will be utilized to

study the cancer cell inhibition by

the novel lectin (SRL)

4. Developed Specific monoclonal antibodies

against toxins of Bacillus thurengenesis

Elisa kit for detection of transgenic

crops.

Industrial training and experience

April 2010

Underwent 8 weeks Industrial training in upstream and downstream processing of recombinant

streptokinase at the premises of Shasun pharmaceutical limited (Biotech division), Velachery

high road, Chennai-600042.

Symposia/Conference/work shop

International Symposium "Bioprocess Industry-Academia Interaction" during 24-27 July,

2011

“Downstream Processing and Validation with an Industrial Approach”:- Training

program jointly organized by CBST and i-FAST on Sept. 3rd-8th, 2012.

“Bio-Downstream Technologies Course (BTC-2013)”. 30th Sep and 4th

Oct. 2013

Publications

1. Vivek Rangarajan, M Rajasekharan, R Ravichandran, K Sriganesh and V

Vaitheeswaran. Pectinase Production from Orange Peel Extract and Dried Orange Peel

Solid as Substrates Using Aspergillus niger. International Journal of Biotechnology

and Biochemistry. (2010)

2. Reena Verma, Ramakrishnan Ravichandran, Naatamai S. Jayaprakash, Ashok Kumar,

Mookambeswaran A.Vijayalakshmi, and Krishnan Venkataraman. Adjuvant poly(N-

isopropylacrylamide) generates more efficient monoclonal antibodies against truncated

recombinant histidinerich protein2 of Plasmodium falciparum for malaria diagnosis.

Biotechnology journal. (2015)

3. R. Ravichandran, Venkatesh Padmanabhan, M. A. Vijayalakhsmi, and N. S.

Jayaprakash. Studies on Recovery of Lactoferrin from Bovine Colostrum Whey Using

Mercapto Ethyl Pyridine and Phenyl Propyl Amine HyperCel™ Mixed Mode Sorbents

Biotechnology and Bioprocess Engineering 20: 148-156 (2015)

Awards/achievements

o Recipient of CSIR, Govt. of India, India, senior research fellowship (SRF) for period

between April 2013 – March 2015.

Personal details

Date of Birth: 06.09.1987

Nationality: Indian

Sex: Male

Languages Known: English, Tamil and Telugu

Permanent address: 8/8 F-2, Anna Street. Gandhi Nagar, Saligramam, Chennai-600093