Results of the 2015 Proficiency Tes7ng SchemeThe Pirbright Institute receives strategic funding...
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www.pirbright.ac.uk The Pirbright Institute receives strategic funding from BBSRC. Results of the 2015 Proficiency Tes7ng Scheme Anna B. Ludi 1 , Ginette Wilsden 1 , Lissie Henry 1 , Valerie Mioulet 1 , Britta Wood 1 , Ashley Gray 1 , Barsha Thapa, Bryony Armson 1 , Julie Maryan, Sarah Belgrave 1 , Donald P. King 1 FMD and SVD Reference Laboratories
Transcript of Results of the 2015 Proficiency Tes7ng SchemeThe Pirbright Institute receives strategic funding...
www.pirbright.ac.ukThe Pirbright Institute receives strategic funding from BBSRC.
Resultsofthe2015ProficiencyTes7ngScheme
Anna B. Ludi1, Ginette Wilsden1, Lissie Henry1, Valerie Mioulet1, Britta Wood1, Ashley Gray1, Barsha Thapa, Bryony Armson1, Julie Maryan,
Sarah Belgrave1, Donald P. King1
FMDandSVDReferenceLaboratories
Ensurecountriesareabletoiden7fyFMDintheircountry§ AchievedbyfeedbackonPTS,trainingandconsulta7on
Harmonisa7onamongstreferencelaboratories
§ Achievedbypresen7ngresultsonhowreferencelaboratoriescompare,distribu7onofreagentsandprotocols
PurposeofProficiencyTes7ngScheme(PTS)
Par7cipants
63 Countries Worldwide
Panel1–Infec7ousmaterialfromonecaseofsuspectedvesiculardiseaseforvirusdetec7on(epithelialsamples)
Panel2–Non-infec7ousmaterialforvirusgenome/an7gendetec7on
Panel3–Non-infec7ousmaterialforFMDserologynon-structuralandstructuralproteins
PanelsDistributed
Panel1–laboratorytestsusedn=21
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VirusIsola7on An7genELISA rRT-PCR
Num
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fLaboratories
SampleIden7fica7on–O/SEA/Mya-98,O/SKR/6/2014
Interpreta7onrRT-PCR–allsamplescorrectlyiden7fied
VirusIsola7on–Onelaboratoryincorrectlyiden7fiedtwosamples
An7genELISA–Onelaboratoryincorrectlyiden7fiedasample
Discussion Cellsusedforvirusisola7on–IB-RS-2,lambkidney,bovinekidney,swinekidney,SK-6,BTY,LFBKαVβ6,BHK-21,ZZR-127
Panel1–Conclusion
Panel2–laboratorytestsusedn=57
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An7genELISA rRT-PCR
Num
bero
flaboratories
SampleIden7fica7on SVD/ITL/8/2008 Lineage-O/ME-SA/Ind-2001 O/SAU/1/2013 Lineage–O/SEA/Mya-98 O/SKR/6/2014 Lineage–A/Asia/Iran-05SIS-10 A/TUR/16/2014 Lineage–Asia1/Sind-08 Asia1/TUR/2/2013 Lineage–SAT2/VIIAlx-12 SAT2/EGY/24/2014
Interpreta7on An7genElisa–Sample5gaveambiguousresultsandsample6mistypedorwasinconclusiveincertainlabsrRT-PCR–Veryconsistentamongalllaboratories,onelaboratoryrequiredfollowup
Discussion TPIan7genELISAkitsarenolongeravailable.NewkitsareavailablethroughapartnershipwithIZSLER–pleasecontactpirbright.kitorders@pirbright.ac.ukformoreinforma7on.
Panel2–Conclusion
Panel3–laboratorytestsusedn=58
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NSP PrioCHECK ID.Vet SPCE LPBE VNT
Num
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Difficultyininterpre7ngELISAcross-reac7vity,par7cularlyLPBE
Discussion Importanceofknowingthelimita7onoftheassaysused
Howisitbeingusedinthefield?Istheserotypealreadyknown?
Panel3–Conclusion
Sample 8
4 mistyped as As1 (1 lab no A system) 1 mistyped as O 2 mistyped as SAT 2 (1 lab no O system) 2 mistyped as SAT 3 (1 lab no A system) 2 positive - unknown serotype
Panel1:Outbreakscenariosofvesiculardiseases(FMDand/orSVD)(infec'ousandnon-infec'ous) Useyourna7onal con7ngencyplanor similardocument to iden7fy theappropriate testsandtes7ngsequence.Thisexerciseisprimarilytoevaluateyouroveralldiagnos7cprocedureratherthan individual test methods. Please use downstream characterisa7on methods, such assequencingiftheyarepartofyourcon7ngencyplan.
Example-Panel1a-Anownerhasreportedsuspicionoffootandmouthdisease,followingashort history of severe lameness and milk drop in one dairy cow, and the development ofblistersinthemouthsoftwocalvesthatarekeptnearby.Thesamplesofthetwocalveshavebeensenttoyourlaboratoryforinves7ga7on.
Panel 2: Quality Assurance FMDV samples to ensure recent strains are detected (non-infec7ous)
ThispanelofvirusesrepresentsrecentFMDVisolatesthatposeacurrentthreattotheEUandelsewhere.Theaimoftheevalua7onistomakesuretheselateststrainscanbedetected.
Panel3:Serologicalsamplesfromanoutbreakscenarioinpanel1 Example–Panel3a–Theoutbreakinscenarios1aand1bhavenowbeencontained.Threeserawerecollectedpostvaccina7on.Pleasetestthemandcommentastowhetherthesamplesarefrominfectedanimals,andifnot,whetherthereisanyevidenceofvaccine-inducedimmunity.
Changestothe2016PTS
Acknowledgement
WorldReferenceLaboratory
CentralServiceUnit
ProficiencyTes7ngSchemeAdvisoryBoard
Youforpar'cipa'nginthePTSandlisteningtothispresenta'on
