Results

1. Colonial Macroscopic Examination

Table 2.1 : Colonial Macroscopic Examination

Yeast Colonial macroscopic Colour Texture Smell Distribution

Pichia spp White Slimy Pungent Even

Hanselula capsulata Clear White Creamy Pungent Colony

Rhodotorula gracilis

Creamy Red Moist Pungent Line

2. Microscopic Examination

Table 2.2 : Microscopic examination

Yeasts Microscopic shape budding cells acrospore

Pichia spp Round Present Absent Hansenula capsulata Round Present Present Rhodotorula gracilis Oval Present Present\

Illustration and Labelled ( Table 2.2 )

a) Pichia spp

Pichia spp Sample Microscope Image Illustration

b) Hanselula capsulate spp

Hanselula Capsulata Sample Microscope Image Illustration

c) Rhodotorula gracillis

Rhodotorula gracillis Sample Microscope Image Illustration

3. Sugar Fermentation

Table 2.3: Acid production from sugar fermentation test

Yeast Acid production from sugar Glucose Lactose Maltose Sucrose

Pichia spp ++ +++ + +++

Hanselula capsulata ++ ++ ++ ++

Rhodotorula gracilis

++ +++ +++ +++

* +++ strong acid production ++ mild acid production + low acid production

Table 2.4: Gas production from sugar fermentation test

Yeast Gas production from sugar Glucose Lactose Maltose Sucrose

Pichia spp √ √ √ √

Hanselula capsulata √ √ √ √

Rhodotorula gracilis

√ √ √ √

* Observe any carbon dioxide gas in durham tube

4. Isolation of yeast from food sample My food sample : Tempe and Tapai Table 2.5 : Colonial Macroscopic Examination from food sample

Yeast Colonial macroscopic Colour Texture Smell Distribution

Tempe White Moist Pungent Dotted

Tapai White Soft furry Pungent Colony

Yeasts Microscopic Examination

Shape budding cells acrospore

Temperound present present

Tapai oval present present

llustration and Labelled ( Table 2.5 )

a) Tempe GYEA

Microscope Image Illustration

b) Tempe PDA

Microscope Image Illustration

b) Tapai GYEA

Microscope Image Illustration

b) Tapai PDA

Microscope Image Illustration