RESTRICTION ENZYME ANALYSIS -...
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RESTRICTION ENZYME ANALYSIS IMBB 2013
Transcript of RESTRICTION ENZYME ANALYSIS -...
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RESTRICTION ENZYME ANALYSIS
IMBB 2013
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RE: recap
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NEBcutter-online tool (free)
• This tool will take a DNA sequence and find the
largest, non-overlapping ORF.
• Can select restriction enzymes that cut the
sequence from a wide range of commercial RE.
• Can customize restriction digestion.
• Can visualize restriction fragments on a virtual
agarose gel.
• http://tools.neb.com/NEBcutter2/
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RFs
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