Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars
description
Transcript of Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars
![Page 1: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/1.jpg)
Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars
By Emily ZhouDepartment of HorticultureSTAT 5606 Spring 2013
![Page 2: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/2.jpg)
Sweet Basil (Ocimum basilicum L.)o A frost sensitive low-growing herb
in family Lamiaceae (mint family)o A summer and annual crop in North
America and native to southern Asia
o Grows a lot in Mediterranean area and South Asia
o More than 60 cultivars
2
![Page 3: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/3.jpg)
Seed Germination Testing Very important to assess quality
6 Sweet Basil Cultivars1. ‘Italian Large Leaf’ (‘ILL’): higher yields2. ‘Italian Large Leaf’ 35X (‘ILL’ 35X): 5+ years old
seeds3. ‘Nufar’: disease (Fusarium) resistant ‘Italian Large
Leaf’ type4. ‘Genovese’: traditional Italian basil5. ‘Genovese Compact Improved’ (‘GCI’): for
containers and pack sales6. ‘Aroma 2’: disease (Fusarium) resistant ‘Genovese’
type
![Page 4: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/4.jpg)
Thermo-gradient Table Creating different temperatures at same time
using the principles of thermodynamics. Wide temperature range from -10 to 85ºC (1.1ºC )
. Often used to test seed germination.
![Page 5: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/5.jpg)
Germination Testing 4 × 25 seeds of cultivar 1 and 2 at each
temperature 2 × 50 seeds of cultivar 3 to 6 at each
temperature Seeds were placed in 11×11×4 cm transparent
covered plastic boxes, on top of two thicknesses of germination blotter paper, the blotters were saturated with 15 ml distilled water
Standard AOSA (The Association of Official Seed Analysts) germination testing procedure, grow in dark in one dimensional thermo-gradient table
![Page 6: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/6.jpg)
Germination Data Analysis Germination percentages
MTG (mean time to germination): ∑(NiTi)/∑(Ni), where Ni is the number of new germinated seeds at time Ti after imbibition calculated from four replications of 25 seeds each (Nerson and Paris, 1988)
![Page 7: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/7.jpg)
Analysis of Covariance of Germination%
![Page 8: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/8.jpg)
Analysis of Covariance of Germination%
![Page 9: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/9.jpg)
Analysis of Covariance of Germination%
Response Surface
![Page 10: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/10.jpg)
Germ% Response Surface Effect by Cultivar
![Page 11: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/11.jpg)
Germ% Response Surface Effect by Cultivar
Cultivars Max Germ (%)
Optimal Temp (°C)
R2 P
1-ILL 35X 84.1 32.2 0.821 <0.0001*
2-Nufar 100 26.4 0.502 <0.0001*
3-Genovese 89.2 28.4 0.632 <0.0001*
4-GCI 100 27.1 0.547 <0.0001*
5-Aroma 2 94.3 31.3 0.757 <0.0001*
6-ILL 99.3 31.5 0.649 <0.0001*
![Page 12: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/12.jpg)
Analysis of Covariance of MTG(days)
![Page 13: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/13.jpg)
Analysis of Covariance of MTG(days)
![Page 14: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/14.jpg)
Analysis of Covariance of MTG(days)
Response Surface
![Page 15: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/15.jpg)
MTG(days)Response Surface Effect by Cultivar
![Page 16: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/16.jpg)
MTG(days)Response Surface Effect by Cultivar
Cultivars Max MTG (days)
Optimal Temp (°C)
R2 P
1-ILL 35X -1.4 30.6 0.934 <0.0001*
2-Nufar -1.0 32.1 0.860 <0.0001*
3-Genovese -1.6 31.6 0.844 <0.0001*
4-GCI -0.3 32.1 0.828 <0.0001*
5-Aroma 2 -0.9 32.2 0.894 <0.0001*
6-ILL -1.4 31.7 0.839 <0.0001*
![Page 17: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/17.jpg)
Future ResearchFind out the optimal germination temp(°C) for each cultivar
High germ% Low MTG(days)
![Page 18: Response Surface Analysis of Seed Germination Percentage and MTG of 6 Basil Cultivars](https://reader035.fdocuments.in/reader035/viewer/2022062810/56815c48550346895dca4b79/html5/thumbnails/18.jpg)
Thank you!