Research Staff National Xenopus · genic membrane GFP frog (by Gary McDowell, 2013 workshop...
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Xenopus Principal Investigators Marko Horb, Director Robert Grainger NXR Administrator Barbara Burbank Frog Lines at the NXR X. laevis: J strain (inbred) Tubb2-GFP Flk1-GFP Col12a1-GFP memGFP H2BmRFP Brainbow X. tropicalis: Nigerian Ivory Coast Otx2-GFP Rax mutant Sleeping beauty transposon EF1a-GFP See mbl.edu/xenopus for a complete list of available lines. Tubb2-GFP Xenopus laevis tadpole, one of the lines maintained at the NXR (by Gary McDowell, 2013 workshop parcipant) R N National Xenopus Resource National Xenopus Resource Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 @XenopusNXR Funding support for the NXR comes from the Office of the Director (OD) and the Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) at the National Institutes of Health. at the Marine Biological Laboratory Woods Hole, Massachusetts, USA Resource National The main Xenopus laevis housing room in the NXR mbl.edu/xenopus The Marine Biological Laboratory (MBL) is dedicated to scientific discovery and improving the human condition through research and education in biology, biomedicine, and environmental science. Founded in Woods Hole, Massachusetts, in 1888, the MBL is a private, nonprofit institution and an affiliate of the University of Chicago. Research Staff Esther Pearl Cristy Salanga Animal Husbandry Staff Katilin Doucette Lynn Ware e-mail: [email protected] E-mail: [email protected]
Transcript of Research Staff National Xenopus · genic membrane GFP frog (by Gary McDowell, 2013 workshop...
Xenopus
Principal InvestigatorsMarko Horb, DirectorRobert Grainger
NXR AdministratorBarbara Burbank
Frog Lines at the NXR
X. laevis:J strain (inbred)Tubb2-GFPFlk1-GFPCol12a1-GFPmemGFPH2BmRFPBrainbow
X. tropicalis:NigerianIvory CoastOtx2-GFPRax mutantSleeping beauty transposonEF1a-GFP
See mbl.edu/xenopus for a complete list of available lines.
Tubb2-GFP Xenopus laevis tadpole, one of the lines maintained at the NXR (by Gary McDowell, 2013 workshop participant)
RNNational Xenopus Resource
National Xenopus ResourceMarine Biological Laboratory7 MBL StreetWoods Hole, MA 02543
@XenopusNXR
Funding support for the NXR comes from the Office of the Director (OD)and the Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) at the National Institutes of Health.
at the Marine Biological LaboratoryWoods Hole, Massachusetts, USA
Resource
National
The main Xenopus laevis housing room in the NXR
mbl.edu/xenopus
The Marine Biological Laboratory (MBL) is dedicated to scientific discovery and improving the human condition through research and education in biology, biomedicine, and environmental science. Founded in Woods Hole, Massachusetts, in 1888, the MBL is a private, nonprofit institution and an affiliate of the University of Chicago.
Research Staff Esther PearlCristy Salanga
Animal Husbandry StaffKatilin DoucetteLynn Ware
e-mail: [email protected]
E-mail: [email protected]
NXR microinjection equipment set up
Workshops
BioinformaticsThe workshop is designed to teach compu-tational skills and methods, primarily aimed at Xenopus wet lab biologists, to enable them to establish a degree of autonomy in processing and computational analysis of experimental data. Researchers are taught a comprehensive set of basic computing skills so that when they leave they are able to work independently and effectively using the newest set of software and tools. Anal-ysis of RNAseq, shotgun proteomics and ChIP-seq data is covered.
Advanced ImagingThe week-long practical workshop is designed to provide hands-on training in advanced imaging in Xenopus in both live and fixed tissue, including computational post-processing. Many embryological techniques for dissecting and preparing embryos for imaging are also taught.
Future Workshops• Genome editing• Transgenesis • Husbandry
The heart of a Cardiac Actin-GFP Xenopus laevis tadpole, photograph colored red (by Kyle Jewhurst, 2013 workshop participant)
Frog Lines
Transgenic Reporter LinesThe NXR breeds, maintains and distributes community generated transgenic lines.
Custom TransgenicsThe NXR creates custom transgenic lines for individual investigators on a made-to-order basis.
Custom KnockoutsThe NXR creates custom knockout lines in X. tropicalis or X. laevis using either TALEN or CRISPR/Cas on a made-to-order basis, including cloning the TALEN and sgRNA constructs.
mbl.edu/xenopus for more information and pricing
High magnification image of the eye of NXR custom trans-genic membrane GFP frog (by Gary McDowell, 2013 workshop participant)
Tyrosinase knockouts:(top) X. laevis J strain TALEN knockout; (bottom) X.tropicalis CRISPR knockout
NXR Resources
The National Xenopus Resource (NXR) is a part of the Eugene Bell Center for Regenerative Biology and Tissue Engineering at the Marine Biological Laboratory (MBL) in Woods Hole, Massachusetts.
The NXR creates, maintains and distributes transgenic and mutant Xenopus lines, runs workshops and hosts visiting researchers.
We offer the following services:
Research Hotel
The NXR Research Hotel offers a fee-based service for visiting scientists. The weekly fee provides researchers with a lab bench and use of equipment, chemicals and frogs.
The NXR also rents tank space and provides animal feeding and general care services to affiliated scientists.
