Research Progress relating to the role of cytochrome P450 in the biosynthesis of terpenoids in...
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Transcript of Research Progress relating to the role of cytochrome P450 in the biosynthesis of terpenoids in...
Research Progress relating to the role of cytochrome P450 in the biosynthesis of
terpenoids in medicinal plants
Yu-Jun Zhao.Qi-Qing.Ping Su.Xin Chen.Xiu-juan.Wei.Lu-QiBy judy mutua
CYP450 Spectrum
• The term P450 is derived from the spectrophotometric peak at the wavelength of the absorption maximum of the enzyme (450 nm) when it is in the reduced state and complexed with CO
Different cytochrome P450 systems
• Most CYPs require a protein partner to deliver one or more electrons.
Examples of plant cytochromesP450 Function Plant SpeciesCYP93E2 β-Balsams alcohol-11-
hydroxylaseBarrel Clover (Medicago truncatula)
CYP71AV1 Amorpha-4,11-diene C-12 oxidase
Artemesia annua
CYP76B6 Geraniol 10-hydroxylase
Madagascar periwinkle (Catharanthus roseus)
CYP725A-like Taxadiene 5-α-hydroxylase
Japanese Yew (Taxus cuspidata)
CYP88D6 Β-Amyrin 11-oxidase Chinese liquorice (Glycyrrhiza uralensis)
Chinese liquorice(Glycyrrhiza uralensis)
• The main active Ingredient is glycyrrhizicacid used to treat cancer& AIDS.
Identification of Licorice β-amyrin 11-oxidase
CYP72 CYP83 CYP88 CYP714 ↘ ↓ ↓ ↙
Conversion of β-amyrin
Transfected insect cells
Challenges in Terpenoid synthesis
• Low abundance in their host organism. • low yields .• high consumption of natural resources.• Chemical synthesis difficult to scale for
industrial production.
Comparison of hostsCharacteristic Bacterial cells Plant cellsSize 1µm 20-50µmShear stress Insensitive SensitiveDoubling Time < 1 hour DaysBatch Fermentation time
Days Weeks
Product Yields High lowPrecursors poor richNative conditions no yesCYP450 enzymes Absent present
Conclusion
• Traditional engineering strategies result to unbalanced carbon flux and accumulation of toxic intermediates that inhibit growth.
• The utilisation of genetic engineering to regulate gene expression and obtain large quantities of terpene compounds should be explored fully.