Research on Different Cholesterol Levels in Chicken

8/12/2019 Research on Different Cholesterol Levels in Chicken

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Prepared By: Rey Angelo F. SalazarHNF152 GH-2L


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Rationale of the study

Cholesterol

Bodys cholesterol needs

Dietary sources of cholesterol

Native Chicken vs. Hybrid Chicken


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OBJECTIVES:

To determine the levels of cholesterol of the meat

and eggs from two varieties of chickens: Philippine

native chicken (Red Junglefowl) and the hybrid

(Mixture of red and gray junglefowl) chicken used in

mass production.

Specific: To distinguish the better chicken variety in terms of

cholesterol content.


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Raw Materials

The native Philippine chicken and the hybrid

chickens meat and 5 eggs each will be obtained in

Los Banos market around January 7, 2011 to

January 10, 2011. The chicken will be dressed,

cleaned and cut in its four major parts; the breasts,

wings, thighs and legs. Both the eggs and chicken will

also be observed for their freshness for optimum

results.


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Preparation

The two variants of chicken will be sliced into its four

major parts; the breast, wings, thighs and legs.

The parts will be boiled then the bones and meat

will be separated. The meats from the different

parts will be placed into properly labeledcontainers and then will we set aside.


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Preparation

The 10 eggs, 5 from the native Philippine chicken

and 5 from the hybrid chicken, will be boiled.

Then, eggs will be cooled and peeled. The yolks will

be separated from the white and will be placed in

containers with appropriate labels.


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Chemical Analysis

Preparation of crude cholesterol

Five egg yolks, five from the native chicken and five from

the hybrid, will be weighed then pulverized in 250-mLlabeled beakers with the aid of a stirring rod. 120mL of

acetone will be added then stirred for 5 minutes, then will

be filtered using vacuum (Extraction and filtration should

be done in an ice bath). Another 120mL of acetone will be

added to the insoluble material. It will be mixed and then

filtered. The insoluble material will be discarded.


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Chemical Analysis


Using simple distillation under steam bath, the acetone

solution will be distilled to obtain a liquid oil residue. Thedistillate will be collected in a receiver cooled in an ice

bath and then transferred in a container labeled

recovered acetone. The flask will be removed from the

water bath after all the acetone had been removed. Then,

40mL of 15% ethanolic KOH (Potassium hydroxide) will be

added. A reflux condenser will be attached and the

mixture will be refluxed for 30 minutes.


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Chemical Analysis


The solution will be cooled to room temperature

and will be poured into a 250-mL separatory

funnel. 30mL of water will be added to the round-

bottom flask, then swirled, and the wash water

will be added to the separatory funnel.


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Chemical Analysis


The water-alcohol solution will be extracted twice with

30mL portions of ether. The ether extracts will becombined then washed with 15mL of distilled water.

The ether solution with 15mL of saturated salt solution will

be shook to lower the water content, and then the solutionswill be separated. The solutions will be filtered with the

use of a cotton plug into a 150-mL beaker and then

evaporated to dryness in the hood using a hot water bath.

(Prolonged heating should be avoided)


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Chemical Analysis

Recrystallization of cholesterol

The crude cholesterol will be dissolved in minimum

amount of hot methanol (~20mL) and will be

quantitatively transferred in a test tube. If the

resulting solution is highly colored, a small amount

of decolorizing carbon will be added, the solutionwill be reheated and the filtered through a fluted

filter paper mounted on a stemless funnel.


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Chemical Analysis

Recrystallization of cholesterol

A 25-mL Erlenmeyer flask will be used to receive

a filtrate. Then the solution will be cooled in an ice

bath and will be left to stand for crystallization.

The crystals will be suction filtered with frequent

washings with cold methanol. The weight andpercent yield of the obtained purified and dried

cholesterol will be determined.

Research on Different Cholesterol Levels in Chicken

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