Research Kit - Belyntic · PP resin PP resin PECinker couplin pp protecting group leaving group...
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Our Research Kit works as the perfect introduction to the world of PEC peptide purification. The ready-to-use package comes with PEC-Linker, cartridges pre- filled with activated filter material, and related consumables. You can choose between three formats to provide you with the ideal kit in a suitable number and scale for your purification needs. Ready-to-use „The time saved working with the Research Kit is precious, enabling tech- nicians to work on other tasks. Also, Belyntic has offered fantastic custo- mer support, with fast communication and great suggestions on how to solve issues to achieve the highest yield and purity.“ Jonas E. Jensen, PhD, Zealand Pharma A/S (Boc)N O O O O O O N H H N Br 2 N3 NO2 Built on Belyntic‘s catch-and-safety-release PEC-Linker RC+ Research Kit 8 x 25 μmol*: meet your research demands Research Kit 8 x 100 μmol*: tackle the elevated scales Research Kit 24 x 10 μmol*: create small purified libraries *referring to synthesis scale Product Highlights: - fast & easy purification - instrument-free parallel purification - low solvent consumption - hands-on explanation on our YouTube-Channel PEC-Linker Activated filter material Reducing Agent Blocking Agent Buffer Manual Product code Price RC+ Agarose100 filled in 24 fritted cartridges DL-Dithiothreitol L-Cysteine Mixture of citric acid-sodium carbonate Included 180484009 540 € RC+ Agarose100 filled in 8 fritted cartridges DL-Dithiothreitol L-Cysteine Mixture of citric acid-sodium carbonate Included 180484008 350 € Research Kit 8 x 25 μmol RC+ Agarose100 filled in 8 fritted cartridges DL-Dithiothreitol L-Cysteine Mixture of citric acid-sodium carbonate Included 180484007 600 € Research Kit 8 x 100 μmol Research Kit 24 x 10 μmol Research Kit
Transcript of Research Kit - Belyntic · PP resin PP resin PECinker couplin pp protecting group leaving group...
Our Research Kit works as the perfect introduction to the world of PEC peptide purification. The ready-to-use package comes with PEC-Linker, cartridges pre-filled with activated filter material, and related consumables. You can choose between three formats to provide you with the ideal kit in a suitable numberand scale for your purification needs.
Ready-to-use
„The time saved working with the Research Kit is precious, enabling tech-nicians to work on other tasks. Also, Belyntic has offered fantastic custo-mer support, with fast communication and great suggestions on how to solve issues to achieve the highest yield and purity.“
Jonas E. Jensen, PhD, Zealand Pharma A/S
(Boc) NO
O
O
O
O
O
NH
HN
Br
2
N3
NO2
Built on Belyntic‘s catch-and-safety-releasePEC-Linker RC+
Research Kit 8 x 25 μmol*: meet your research demands Research Kit 8 x 100 μmol*: tackle the elevated scales Research Kit 24 x 10 μmol*: create small purified libraries
*referring to synthesis scale
Product Highlights:
- fast & easy purification- instrument-free parallel purification - low solvent consumption - hands-on explanation on our YouTube-Channel
PEC-Linker
Activated filter material
Reducing Agent
Blocking Agent
Buffer
Manual
Product code
Price
RC+
Agarose100 filled in 24 fritted cartridges
DL-Dithiothreitol
L-Cysteine
Mixture of citric acid-sodium carbonate
Included
180484009
540 €
RC+
Agarose100 filled in 8 fritted cartridges
DL-Dithiothreitol
L-Cysteine
Mixture of citric acid-sodium carbonate
Included
180484008
350 €
Research Kit 8 x 25 μmol
RC+
Agarose100 filled in 8 fritted cartridges
DL-Dithiothreitol
L-Cysteine
Mixture of citric acid-sodium carbonate
Included
180484007
600 €
Research Kit 8 x 100 μmol Research Kit 24 x 10 μmol
Research Kit
1. Couple the PEC-Linker after SPPS2. Cleave and elute the peptide3. Precipitate and dissolve4. Catch on activated filter material5. Wash away impurities6a. Reduce the PEC-Linker to enable safety-release of peptide6b. Release your purified peptide with weak acids
Belyntic‘s Peptide Easy Clean (PEC) technology breaks barriers in peptide purification. The key for a universal appli-cation is our novel reductively cleavable PEC-Linker RC+. The target peptide is covalently captured on our high-capacity activated filter material via robust oxime ligation. The safety-catch functionality allows the traceless release of the purified peptide without the formation of by-products.
2. TFA cleavage
3. Dissolution4. Catch
activated filter material
purified peptide
P T I D
I D
H N²
HN
P E
PG
Ac T E
PG
E
PG
capping with Ac O2
amino acid coupling
Ac
SPPSresin
SPPSresin
1. PEC-Linker coupling
pNp
protecting group leaving groupp-Nitrophenol
pNp
PG
PG
P T I D
I D
P E
PG
E
PG
E
PG
SPPSresin
Ac T
PG
P T I D
I D
P E
E
E
H N²
Ac T
Dithiothreitol
6a. DTT
P T I D
I D
P E
E
E
N
Ac T
P T I DP E E
N
H N²
ID
EA
cT 5. W
ash
DTT (ox)
6b. H+ Release
1,6-elimination
H N² CO2
P T I DH N² P E E
0ur Technology
+
SPPS
1. 2. 3. 4.
purifiedpeptide
5. 6a. 6b.
Richard-WiIlstaetter-Str. 1112489 BerlinGermany
Changing the Tide in Peptides @belyntic#belynticBelyntic
0049 30 [email protected]
