Replicacion y Recombinacion DNA
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Transcript of Replicacion y Recombinacion DNA
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Factores de replicación y combinación que contribuyen a la
reparación del DNA
Sergio UribePrograma de Doctorado en Ciencias Médicas
Universidad Austral de Chile
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Reparación del DNA
Development of cancer stem cells from the normal stem cells and progenitor cells. Accumulation of DNA errors in normal stem cells or progenitor cells are activated to generate a cancer stem cells (CSCs) that further generate a primary tumor constituting CSCs and other tumor cells. Vaish Molecular Cancer 2007 6:26 doi:10.1186/1476-4598-6-26
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From:Â DNA Repair
Copyright © 2002, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter; Copyright © 1983, 1989, 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson .
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From:Â DNA Repair
Copyright © 2002, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter; Copyright © 1983, 1989, 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson .
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From:Â DNA Repair
Copyright © 2002, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter; Copyright © 1983, 1989, 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson .
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From:Â DNA Repair
Copyright © 2002, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter; Copyright © 1983, 1989, 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson .
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Materials and methods
Yeast strains and plasmids
Growing conditions, cell cycle arrests, and drug treatments
Spot assays of drug sensitivity
Protein techniques
FACS analysis
Extraction of replication intermediates and the 2D gel procedure
Quantification of replication intermediates
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Materials and methods
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Results
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Results The role of the HR factors Rad55 and Rad59 in the
formation of template switch intermediates Rad55, but not Rad55 phosphorylation by Rad53 or
Rad59, is required for template switch replication The effect of the RPA mutation, rfa1-t11, on template
switch RPA, promoting the strand invasion step of homologous
recombination, is required for template switch replication
The Exo1 exonuclease is required for efficient damage-induced template switch events
Exo1 contributes to damage-bypass replication by template switch
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TLS polymerases are not required for template switch–mediated damage bypass Translesion synthesis polymerases do not
contribute to the DNA synthesis step of template switch.
Differential requirements for replicative polymerases in template switch replication Polδ but not Polε, is required for template switch
replication
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...understanding how different DNA synthetic and repair demands are orchestrated to prevent the accumulation of DNA damage and maintain chromosomal stability has important implications for enhancing our knowledge of how cells are protected from cancer-causing alterations
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Referencias
Maresca M, Erler A, Fu J, Friedrich A, Zhang Y, Stewart AF. Single-stranded heteroduplex intermediates in lambda Red homologous recombination. BMC Molecular Biology 2010, 11:54
Vanoli F, Fumasoni M, Szakal B, Maloisel L, Branzei D. Replication and recombination factors contributing to recombination-dependent bypass of DNA lesions by template switch. PLoS Genet. 2010 Nov 11;6(11):e1001205.
Alberts B et al. Molecular Biology of the Cell, 5th edition. Ch 5. DNA Replication, Repair, and Recombination
Vaish M. Mismatch repair deficiencies transforming stem cells into cancer stem cells and therapeutic implications. Mol Cancer. 2007 Apr 2;6:26.