Relative Fluorescence
description
Transcript of Relative Fluorescence
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Rel
ativ
e Fl
uore
scen
ce
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Fo
Fm
Fv
PSII
Flashlet Number
FRR DATA
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Rel
ativ
e Fl
uore
scen
ce
Flashlet Number
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Rel
ativ
e Fl
uore
scen
ce
Flashlet Number
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0 1000000 2000000 3000000 4000000 5000000 6000000 7000000 8000000
Time (s)
Rel
ativ
e Fl
uore
scen
ce
120o E
5o
15o
25o
35o
5o
15o
25o
160o W160o E 120o W
5o
15o
25o
35o
5o
15o
25o
140o E 80o W
Nitrate C
oncentrations (M)
8
6
4
2
0180o 140o W 100o W
Hawaii
1994‘OliPac’
2001C
2000
1995 IronExII
2001B
2001A
2006
2003
2002 2001D‘GasEx’
26 short-term (18-30 h) enrichment experiments (control, +NO3, +NH4, +PO4, +Fe)
Depth-resolved time-series stations
Tropical Pacific Study
>140,000 Variable fluorescence (FRRf) Incident PARChlorophyll & HPLC beam attenuation @ 660 nmMacronutrients spectral attenuation & absorption (AC9)Fe concentrations cDOMPhotosynthesis-Irradiance (14C) particulate backscattering coefficientsPhytoplankton absorption
web.science.oregonstate.edu/ocean.productivity/
2005
Sequence Number
Fo
& F
m (
rela
tive
)P
AR
(uE
in)
2006A
2005
2006B
2007
Sequence Number
Fv/
Fm
(re
lati
ve) P
AR
(uE
in)
2006A
2005
2006B
2007
Sequence Number
Sigm
a P
SII
(rel
ativ
e)P
AR
(uE
in)
2006A
2005
2006B
2007
120o E
5o
15o
25o
35o
5o
15o
25o
160o W160o E 120o W140o E 80o W180o 140o W 100o W
Hawaii
Time12:00 0:00 12:00 0:00 12:00
PQ
turn
over
tim
e
Low nitrate, low biomass zone High nitrate, high biomass zone
Latitude
0
5
10
15
20
160N 80140 120 90 60 2040 00 20 8040 S
Slow PQ turnover
Rapid PQ turnover
Fluorescence properties in the tropical Pacific:
25
PQ p
ool e
lect
ron
turn
over
tim
e
0
10
20
A
Time12:00 0:00
0.1
0.2
0.3
12:00 0:00 12:00
F v/F m
Nocturnal Decreases
The nocturnal decrease:
PSI
Cytb6 f
c553
PQ
PQH2
PSII
Fdx
Ter
min
alox
idas
e
PQ
PQH2
Ndh
NADH
Nig
htt
ime
e
e
e e
Slight electron backtransfer
e
FRRf
Iron stress
O2H20
12
MAJOR electron backtransfer
1. Photosynthesis OFF, Respiration ON2. Increasing PQ pool reduction and electron backtransfer3. Photosynthate supply running out4. PSI & PSII turnover at dawn
Large nocturnal decreases require Fe stress, modest or better growth rates, but not high macronutrients
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Time Series 2 8S, 125W
Time Series 3 5S, 140W
Time Series 4 - 0Eq, 140W
Blue =
day, sunrise, sunsetP
ink = night
14-C data
Dawn maxima:
PSI
Cytb6 f
c553
Fdx
PQPQH2 e
e
H20 O212 + 2 H
PSII
e
Isi
Iron stressIron stress
FRRf fluorescence
Latitude
Fv/
Fm
0.1
0.2
0.3
0.4
0.5
160N 80140 120 100 60 2040 00 20 8040 S
High Dawn Maxima
Low Dawn Maxima
Low dawn maxima require Fe stress, high macronutrients, but not rapid growth rates
Nocturnal PSII cross-section decreases:
Latitude
PS
II (2 p
hoto
n-1)
100
200
400
0
300
160N 80140 120 100 60 2040 00 20 8040 S
‘State transitions’
Large state transitions require Fe stress, significant prokaryotic biomass, but not high macronutrients
PQ turnover:
Long PQ turnover times require Fe stress-induced decreases in down-stream components
175o W 135o W155o W 95o W115o W165o E
0%
20%
40%
60%
Nocturnal decrease in F
v /Fm
175o W 135o W155o W 95o W115o W165o E
0
0.2
0.4
0.6
Daw
n Fv /F
m maxim
um
175o W 135o W155o W 95o W115o W165o ELongitude
I
IIIII
I
I
I
Lat
itud
e
5o S
15o S
25o S
15o N
25o N
35o N
5o N
Lat
itud
e
5o S
15o S
25o S
15o N
25o N
35o N
5o N
Lat
itud
e
5o S
15o S
25o S
15o N
25o N
35o N
5o N
POC / PON
y = 39.85x + 12.355
R2 = 0.9983
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y = 38.813x + 3.814
R2 = 0.9994
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80
90
0 0.5 1 1.5 2 2.5
y = 38.001x + 5.472
R2 = 0.9984
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Sequential Station
Bla
nks
2006A
Sequence Number
PO
N (
ug L
-1)
2006A
2005
2006B
Sequence Number
PO
C (
ug L
-1)
2006A
2005
2006B
Sequence Number
C:N
rat
io
2006A
2005
2006B
Turner fluorometer
Sequence Number
2006A
2005
2006B
2007
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0.3
0.35
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0.3
0.35
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