Regulation of biomass growth and carbon partitioning in poplar – molecular characterization of a...
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POSTER PRESENTATION Open Access Regulation of biomass growth and carbon partitioning in poplar – molecular characterization of a candidate gene Cintia Ribeiro 1* , Evandro Novaes 2 , Christopher Dervinis 3 , Matias Kirst 3 From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery Arraial d Ajuda, Bahia, Brazil. 26 June - 2 July 2011 Improvement of plant feedstock for bioenergy produc- tion can be achieved by modifying wood chemical prop- erties and increasing biomass productivity. We previously identified a candidate gene for carbon parti- tioning and growth on chromosome 13 (cpg13) of poplar. Cpg13 was identified as the regulator of carbon partition and growth within a QTL interval that explains 56% of the variation in cellulose to lignin ratio, as well as 20-25% of the heritable variation in biomass. Putative homologues of cpg13 in Arabidopsis are annotated as proteins of unknown function; therefore, the functional characterization of cpg13 is essential. At present, evi- dence of the functional role of cpg13 is being obtained by the analysis of poplar transgenic plants transformed with RNAi, overexpression and GFP-fused – cpg13 con- structs. Preliminary data indicates that down-regulating cpg13 positively impacts growth, while a negative effect is detected in lines overexpressing cpg13. Transgenic poplar 35S::cpg13:GFP shows localization in cell wall, consistent with in silico predictions. Comparative geno- mics indicate moderate similarity with methyltransfer- ase. Analysis of the impact of differentially regulating cpg13 on lignin and cellulose is currently under way in the mutants. Furthermore, attempts to purify the cpg13 protein are in progress, to define its role through a ser- ies of biochemical function assays. Author details 1 Plant Molecular and Cellular Biology Graduate Program, University of Florida, Gainesville, FL, 32611, USA. 2 Escola de Agronomia e Eng. de Alimentos, Federal University of Goias, Goias, Brazil. 3 School of Forest Resources and Conservation, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, USA. Published: 13 September 2011 doi:10.1186/1753-6561-5-S7-P122 Cite this article as: Ribeiro et al.: Regulation of biomass growth and carbon partitioning in poplar – molecular characterization of a candidate gene. BMC Proceedings 2011 5(Suppl 7):P122. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit * Correspondence: [email protected] 1 Plant Molecular and Cellular Biology Graduate Program, University of Florida, Gainesville, FL, 32611, USA Full list of author information is available at the end of the article Ribeiro et al. BMC Proceedings 2011, 5(Suppl 7):P122 http://www.biomedcentral.com/1753-6561/5/S7/P122 © 2011 Ribeiro et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Transcript of Regulation of biomass growth and carbon partitioning in poplar – molecular characterization of a...
POSTER PRESENTATION Open Access
Regulation of biomass growth and carbonpartitioning in poplar – molecularcharacterization of a candidate geneCintia Ribeiro1*, Evandro Novaes2, Christopher Dervinis3, Matias Kirst3
From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and DeliveryArraial d Ajuda, Bahia, Brazil. 26 June - 2 July 2011
Improvement of plant feedstock for bioenergy produc-tion can be achieved by modifying wood chemical prop-erties and increasing biomass productivity. Wepreviously identified a candidate gene for carbon parti-tioning and growth on chromosome 13 (cpg13) ofpoplar. Cpg13 was identified as the regulator of carbonpartition and growth within a QTL interval that explains56% of the variation in cellulose to lignin ratio, as wellas 20-25% of the heritable variation in biomass. Putativehomologues of cpg13 in Arabidopsis are annotated asproteins of unknown function; therefore, the functionalcharacterization of cpg13 is essential. At present, evi-dence of the functional role of cpg13 is being obtainedby the analysis of poplar transgenic plants transformedwith RNAi, overexpression and GFP-fused – cpg13 con-structs. Preliminary data indicates that down-regulatingcpg13 positively impacts growth, while a negative effectis detected in lines overexpressing cpg13. Transgenicpoplar 35S::cpg13:GFP shows localization in cell wall,consistent with in silico predictions. Comparative geno-mics indicate moderate similarity with methyltransfer-ase. Analysis of the impact of differentially regulatingcpg13 on lignin and cellulose is currently under way inthe mutants. Furthermore, attempts to purify the cpg13protein are in progress, to define its role through a ser-ies of biochemical function assays.
Author details1Plant Molecular and Cellular Biology Graduate Program, University ofFlorida, Gainesville, FL, 32611, USA. 2Escola de Agronomia e Eng. deAlimentos, Federal University of Goias, Goias, Brazil. 3School of Forest
Resources and Conservation, Institute of Food and Agricultural Sciences,University of Florida, Gainesville, FL, 32611, USA.
Published: 13 September 2011
doi:10.1186/1753-6561-5-S7-P122Cite this article as: Ribeiro et al.: Regulation of biomass growth andcarbon partitioning in poplar – molecular characterization of acandidate gene. BMC Proceedings 2011 5(Suppl 7):P122.
Submit your next manuscript to BioMed Centraland take full advantage of:
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
• Inclusion in PubMed, CAS, Scopus and Google Scholar
• Research which is freely available for redistribution
Submit your manuscript at www.biomedcentral.com/submit
* Correspondence: [email protected] Molecular and Cellular Biology Graduate Program, University ofFlorida, Gainesville, FL, 32611, USAFull list of author information is available at the end of the article
Ribeiro et al. BMC Proceedings 2011, 5(Suppl 7):P122http://www.biomedcentral.com/1753-6561/5/S7/P122
© 2011 Ribeiro et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.
