References Prof. RAKESH BHATNAGAR Ph.D., F.N.A,, F.N.A.Sc ... · 2018-TILL DATE Vice Chancellor...
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Prof. RAKESH BHATNAGAR Ph.D., F.N.A,, F.N.A.Sc., F.A.Sc. Vice Chancellor, Banaras Hindu University, Varanasi J.C. Bose National Fellow Molecular Biology and Genetic Engineering Laboratory School of Biotechnology Jawaharlal Nehru University New Delhi - 110067, India Contact info: Phone: 011-26704079, 26704715 Mail: [email protected] [email protected] [email protected] DATE OF BIRTH: 11-07-1951 EDUCATION: Ph.D. (1978), Department of Biochemistry National Sugar Institute, Kanpur, India. ADMINISTRATIVE EXPERIENCE: 2018-TILL DATE Vice Chancellor Banaras Hindu University, Varanasi 2013 - 2015 Dean School of Biotechnology Jawaharlal Nehru University, New Delhi. 2012 – 2013 Vice Chancellor Kumaun University Nainital, Uttarakhand 2011 - 2012 Director Academic Staff College Jawaharlal Nehru University, New Delhi. 2009 - 2011 : Director Advanced Instrumentation Research Facility Jawaharlal Nehru University, New Delhi. 2007 - 2012 : Chairman Intellectual Property Management Cell Jawaharlal Nehru University, New Delhi.
Transcript of References Prof. RAKESH BHATNAGAR Ph.D., F.N.A,, F.N.A.Sc ... · 2018-TILL DATE Vice Chancellor...
References
Prof. RAKESH BHATNAGAR
Ph.D., F.N.A,, F.N.A.Sc., F.A.Sc.
Vice Chancellor, Banaras Hindu University, Varanasi
J.C. Bose National Fellow
Molecular Biology and Genetic Engineering Laboratory
School of Biotechnology
Jawaharlal Nehru University
New Delhi - 110067, India
Contact info:
Phone: 011-26704079, 26704715
Mail: [email protected]
one
DATE OF BIRTH: 11-07-1951
EDUCATION: Ph.D. (1978), Department of Biochemistry
National Sugar Institute, Kanpur, India.
ADMINISTRATIVE EXPERIENCE:
2018-TILL DATE Vice Chancellor
Banaras Hindu University, Varanasi
2013 - 2015 Dean
School of Biotechnology
Jawaharlal Nehru University, New Delhi.
2012 – 2013 Vice Chancellor
Kumaun University
Nainital, Uttarakhand
2011 - 2012 Director
Academic Staff College
Jawaharlal Nehru University, New Delhi.
2009 - 2011 : Director
Advanced Instrumentation Research Facility
Jawaharlal Nehru University, New Delhi.
2007 - 2012 : Chairman
Intellectual Property Management Cell
Jawaharlal Nehru University, New Delhi.
2001 - 2003 : Chairman
1993 - 1995 Centre for Biotechnology
Jawaharlal Nehru University, New Delhi.
1990 - 1993 : Coordinator
Bioinformatics Centre
Jawaharlal Nehru University, New Delhi.
PROFESSIONAL EXPERIENCE:
1997 onwards Professor
School of Biotechnology
Jawaharlal Nehru University, New Delhi.
2010 -2013 Faculty in U.S.-India Joint Research Training Program
Seattle Biomedical Research Institute Seattle WA, USA
1989 - 1997 Associate Professor
Centre for Biotechnology
Jawaharlal Nehru University, New Delhi.
1987 - 1989 Senior NRC Associate, USAMRIID,
Fredrick MD, USA.
1986 - 1987 Visiting Associate, NHLBI, NIH,
Bethesda, MD, USA.
1985 - 1986 Visiting Assistant Prof., Lab. Connective
Tissue, Biochemistry, CHU, Caen, FRANCE.
1983 - 1985 Assistant Professor of Biochemistry
Post Graduate Institute of Med. Education &
Research, Chandigarh.
1982 - 1983 Pool Officer, Department of Biochemistry
V. P. Chest Institute, University of Delhi.
1979 - 1982 Scientist (Wiessenschaftliecher Angeschtelter)
Institute of Biochemistry and Molecular Biology,
Freiburg University, Freiburg, GERMANY.
TECHNOLOGY TRANSFER
We have transferred technology for making “Genetically Engineered Vaccine against
Anthrax” to M/s. Panacea Biotec Ltd., New Delhi at a total technology transfer fee of Rs.
2.00 crores and a royalty of 5% for 10 years.
TECHNOLOGY TRANSFER AGREEMENT BETWEEN JAWAHALAL NEHRU
UNIVERSITY AND M/S PANACEA BIOTECH LTD. (THROUGH BCIL, NEW DELHI)
SIGNED IN PRESENCE OF DR. MANJU SHARMA
New Anthrax Vaccine Developed in India
Pallava Bagla
for National Geographic News
November 6, 2001
The war against terrorism may have a new weapon. Indian scientists announced November 5 that they have developed a safer and cheaper
anthrax vaccine. The new vaccine is less toxic and longer lasting
than the currently available vaccine, said its developers Rakesh Bhatnagar, chairman of the Center for Biotechnology at Jawaharlal Nehru University (JNU), and Yogendra Singh, a scientist at the Center for
Biochemical Technology, New Delhi. Anthrax is an acute infectious disease caused by the spore-forming bacterium Bacillus anthracis. It occurs commonly
among cattle, sheep, goats, camels, and other herbivores but it can also occur in humans when they are exposed to infected animals or tissue from infected animals. It has recently become a weapon in the arsenal of terrorists.
"Four people have died from anthrax infections in the United States over the past several weeks, and in some of these cases, bioterrorism-quality anthrax has been the cause," said Elaine Fuchs, a molecular geneticist at the Howard Hughes Medical Institute in Chicago and president of the American Society of Cell Biology (ASCB).
The human anthrax vaccine on the market today was developed at the behest of the Pentagon, and is only available for use by American troops. The current vaccine requires 18 months to take effect and can have toxic side effects. Many soldiers have opted to face disciplinary action rather than subject themselves to the six-course dose.
"The new research by Dr. Bhatnagar is extremely promising, precisely because his newly-engineered anthrax proteins
might avoid the side-effects that plagued the previous vaccine," said Katherine Wilson, a biologist at Johns Hopkins University School of Medicine, Baltimore. Bhatnagar's work was scheduled to be released at the ASCB annual conference in December. The association
decided to break its long tradition of keeping the contents of research papers secret until they have been presented because of the vaccine's potential impact on public health.
Developing a Vaccine The vaccine is the result of seven years of work, funded in part by the Indian Department of Biotechnology. Led by Bhatnagar, the research team created harmless mutant forms of the three key proteins that together make anthrax
fatal. The genes for the mutated proteins were introduced into host organisms, where they reproduced. The mutant proteins were then purified to ensure that reactivity and side effects of the vaccine were minimized.
Once the researchers were confident that they could reproduce the protective antigens on a laboratory scale, Bhatnagar's team took the production to near-industrial scale. A five-liter capacity fermenter can now produce
approximately five grams of protective antigen per liter, said Bhatnagar. One gram of the antigen can supply millions of vaccine shots.
The genetically engineered anthrax antigen can also be modified to create an anthrax vaccine for use in livestock. Moving Toward Commercialization
The vaccine will soon undergo toxicity testing, followed by human trials. While there are many hurdles still to be overcome, the Indian team is optimistic that the new vaccine will stand the scrutiny.
India's science minister Murli Manohar Joshi, told a crowded press conference Monday that India is fast-tracking the regulatory procedure and hopes to have the vaccine available in six to nine months to ensure that the "masses get the much needed vaccine on time."
Panacea Biotech, an Indian biotechnology company, has purchased the anthrax vaccine technology and will produce the vaccine commercially.
"If all goes on schedule we hope to market the new anthrax vaccine worldwide in the next six to nine months at a very nominal price," said Rajesh Jain, director of Panacea Biotech. The final pricing will be worked out once the product goes through regulatory clearance, he said.
"Given the urgent and unexpected need for new and improved methods of treating and preventing anthrax infections, research on anthrax vaccines has surfaced to the forefront of biomedical research in the world," said Fuchs. "Dr. Bhatnagar's research has the potential for contributing to these efforts."
http://news.nationalgeographic.com/news/2001/11/1105_anthraxvaccine.html
© 1996-2008 National Geographic Society. All rights reserved.
ICMR AWARD GIVEN BY Ms. SUSHMA SWARAJ
PRESIDENT’S AWARD FOR BEST INNOVATION (2016), CONFERRED
BY SHRI PRANAB MUKHERJEE, PRESIDENT OF INDIA.
Created a BSL-3 (Bio-Safety Laboratory level-3) for the first time in a
University.
AWARDS/FELLOWSHIPS/ RECOGNITIONS/ACHIEVEMENTS:
President’s Award for Innovation (2016), conferred by the President of India.
Fellow, Indian National Science Academy, New Delhi, India.
J.C. Bose National Fellow, New Delhi, India.
Fellow, National Academy of Sciences, Allahabad, India.
Fellow, Indian Academy of Sciences, Bangalore, India.
Indian Council of Medical Research (ICMR) award for outstanding research work in the
field of Immunology (Development of recombinant anthrax vaccine) for the year 2001,
received on 14th Dec. 2003.
Ranked 7th among the top ten eminent researchers publishing commendable research
papers on anthrax. (Open source Global Anthrax Research Literature 2006)
All India Biotech Association Award 2001-2002, in recognition of achievements in
Promotion of Biotechnology, In the Area of Biotechnology for Health Care.
CONSULTANCY: In addition to teaching and research, I have been providing
consultancy to Biotechnology/ Pharmaceutical industry during the last fifteen years.
REFERENCES:
Prof. Yogendra Alagh
Former Vice Chancellor
Minister of Science & Technology &Section
Former Chancellor Nagaland University
Dr. Art Friedlander
USAMRIID, Fredrick MD, USA
Prof. Sudhir Kumar Sopory
Former Vice Chancellor
Jawaharlal Nehru University
New Delhi – 110067
Dr. Stephen Leppla
Chief, Microbial Pathogenesis,
Building 33, Room 1W20B4
33 North Drive, Bethesda, MD 20892 [email protected]
Prof. N.K. Ganguly
Former Director General
Indian Council of Medical Research
New Delhi – 110 067 [email protected]
Prof. Karl Decker
Professor Emeritus
Institute of Biochemistry & Molecular Biology
Freiburg University, Germany
Prof (Dr) Seyed E. Hasnain
Vice Chancellor Jamai Humdard University, New Delhi
REFERENCEReferences
No. of Research Scholars (Ph.D) successfully guided:
S.
No. Name of scholar Affiliated University/ Institute
Awarded /
Under
guidance
Main Guide or
Co-Guide
1 Uttam Garg PGI Chandigarh Awarded Main Guide
2 Deotosh Kr Surendra
Singh
Jawaharlal Nehru University Awarded Main Guide
3 Adhuna Jawaharlal Nehru University Awarded Main Guide
4 Puran Singh Sijwali Jawaharlal Nehru University Awarded Main Guide
5 Pankaj Gupta Jawaharlal Nehru University Awarded Main Guide
6 Smriti Batra Jawaharlal Nehru University Awarded Main Guide
7 Vibha Chauhan Jawaharlal Nehru 8University Awarded Main Guide
8 Aparna Singh Jawaharlal Nehru University Awarded Main Guide
9 Sharik Rahil Khan Jawaharlal Nehru University Awarded Main Guide
10 Syed Mohseen Waheed Jawaharlal Nehru University Awarded Main Guide
11 Praveen Kumar Jawaharlal Nehru University Awarded Main Guide
12 Samer Singh Jawaharlal Nehru University Awarded Main Guide
13 Md. Azhar Aziz Jawaharlal Nehru University Awarded Main Guide
14 Puneet Khandelwal Jawaharlal Nehru University Awarded Main Guide
15 A.Krishnanachari Jawaharlal Nehru University Awarded Main Guide
16 Subhash Chandra Jawaharlal Nehru University Awarded Main Guide
17 Megha Gupta Jawaharlal Nehru University Awarded Main Guide
18 Sheeba Alam Jawaharlal Nehru University Awarded Main Guide
19 Mohan Joshi Jawaharlal Nehru University Awarded Main Guide
20 Suchi Midha Jawaharlal Nehru University Awarded Main Guide
21 Jitendra Singh Jawaharlal Nehru University Awarded Main Guide
22 Manpreet Kaur Jawaharlal Nehru University Awarded Main Guide
23 Jyotsana Jawaharlal Nehru University Awarded Main Guide
24 Parul Kulshrestha Jawaharlal Nehru University Awarded Main Guide
25 Kanchan Sinha Jawaharlal Nehru University Awarded Main Guide
26 Divya Goe Jawaharlal Nehru University Awarded Main Guide
27 Manish Mishra Jawaharlal Nehru University Awarded Main Guide
28 Preeti Singh Jawaharlal Nehru University Awarded Main Guide
29 Gurudutta Panda Jawaharlal Nehru University Awarded Main Guide
30 Shashikala Verma Jawaharlal Nehru University Awarded Main Guide
31 Rajni Garg Jawaharlal Nehru University Awarded Main Guide
32 Deeksha Tripathi Jawaharlal Nehru University Awarded Main Guide
33 Alisha dhiman Jawaharlal Nehru University Awarded Main Guide
34 Vikas Somani Jawaharlal Nehru University Awarded Main Guide
35 Somya Aggarwal Jawaharlal Nehru University Awarded Main Guide
36 Damini Singh Jawaharlal Nehru University Awarded Main Guide
37 Neha Chaudhary Jawaharlal Nehru University Awarded Main Guide
38 Jaishree Garhyan Jawaharlal Nehru University Awarded Main Guide
39 Monisha Gopalani Jawaharlal Nehru University Awarded Main Guide
40 Akanksha Jawaharlal Nehru University Awarded Main Guide
41 Amit Rahi Jawaharlal Nehru University Awarded Main Guide
42 Divya Khandari Jawaharlal Nehru University Awarded Main Guide
43 Himanshu Jawaharlal Nehru University Awarded Main Guide
44 Surender Mohan Jawaharlal Nehru University Awarded Main Guide
45 Anshu Malik Jawaharlal Nehru University Awarded Main Guide
46 Sonal Gupta Jawaharlal Nehru University Awarded Main Guide
47 Mohit Mishra Jawaharlal Nehru University Under guidance Main Guide
48 Vatika Gupta MDU University, Rohtak Awarded Co Guide
49 Shikha Joon Netaji Subhas Institute of
Technology
Awarded Co Guide
50 Hemant Joshi Jawaharlal Nehru University Under guidance Co Guide
51 Manish Gupta ICGEB Awarded Co Guide
52 Harish Kumar ICGEB Awarded Co Guide
53 Shashi Kant ICGEB Awarded Co Guide
RESEARCH PROJECTS–
ON-GOING
Sl. Organization’s
name
Name of project Duration of
project
Amount
of
Grant (in
lakhs)
1 Department of
Biotechnology
Study of in-depth genetic heterogeneity
with respect to resistome and
compensatory adaption of MDR Mtb
clinical strains inside BM- Mesenchymal
stem cells circulating in the North- East
Region
* 2019-2022
*Project
sanctioned.
Grant yet to be
received
300.00
2 Department of
Biotechnology
Upgradation of BSL-3, for studying
dangerous pathogens with special
references to Bacillus anthracis as well as
to provide BSL-3 Facility to NER
institutions and training of researchers of
this region
2017-2020 314.679
3 Department of
Science Technology
J.C. Bose Fellowship 2016-2021 95.00
4 UPOE-II Understanding Physiology of B.
anthracis using a genomics, proteomics
and system Biology approach
2014-2019 11.00
5 Indian Council of
Medical Research
Design and Evaluation of Inhibitors
Abrogating Surface Bound
Mycobacterium tuberculosis a Enolase
Interaction with Extracellular Matrix
Proteins for Tuberculosis Control
2015-2018 59.00
6 Department of
Biotechnology
Development of recombinant Omp25
based vaccine against Brucellosis for
Human.
2014-2018 103.00
COMPLETED 7 Indian Council of
Medical Research
Development of Nano particulate anti
tubercular Drug delivery system for
macrophage receptor specific
targeting through oral and respiratory
route: Phase I study.
2015-2017 20.00
8 Department of
Biotechnology
Operation and Maintenance of P3
Facility (BSL3) for studying
dangerous pathogens with special
reference to anthrax causing
pathogens Bacillus anthracis-
[11547]”
2012-2017 114.7
9 Indian Council of
Medical Research
Role of multifunctional plasminogen
biding glyceraldehyde 3 phosphate
dehydrogenase of bacillus anthracis.
2012-2017 61.05
10 Department of
Biotechnology
Characterization and adjuvant
potential of Poly-a- L-Glutamine of
Mycobacterium tuberculosis H37Rv.
2012-2016 47.32
11 Indian Council of
Medical Research
Development of novel antibiotics of
control infectious disease in
pathogenic bacteria through induction
of programmed cell death.
2012-2015 28.97
12 DST in
collaboration with M/s.
Lal Path Labs Pvt. Ltd.
Development of Diagnostic Kit for
detection of Mycobacterium
tuberculosis complex &
Mycobacterial species (by whole
genome sequencing).
2011-2014 316.946 (219.342+
97.604)
13 Department of
Biotechnology
Identification and Characterization of
immunodominant B. Cell Epitopes,
that confer protective Immunity
against Anthrax.
2010-2014 54.104
14 Department of
Science and Technology
Development of transgenic
BrassicaJuncea expressing normal and
mutated edema factor gene for the
development of vaccine against
anthrax
2010-2013 34.70
15 Council of
Scientific Industrial
Research
Role of Heat Shock Protein as
immunodulators and their role in
immunity against Bacillus anthracis
infection.
2011-2014 29.84
16 DST in
collaboration with
Parental Biotech Ltd.
Rabies DNA Vaccine: Bioprocess
optimization & immunogenecity
studies.
2009-2013 223.92
(128.94
+94.98)
17 Indian Council of
Medical Research
Cloning expression and molecular
characterization of alanine racemase
of Bacillus anthracis.
2009-2012 15.34
18 Indian Council of
Medical Research
(Indo-German)
Genome wide reconstruction and
analysis of Bacillus anthracis
metabolic network and its
transcriptional regulation
2009- 2011 20.71
19 DST in
collaboration with
Arbro Pharmaceuticals
Ltd.
Development of a bispecific single
chain antibody neutralizing edema
toxin and lethal toxin of anthrax
2008-2011 177.454 (159.502
+ 17.952)
20 Department of
Biotechnology
Creation of P3 facility for studying
dangerous pathogens with special
reference to anthrax causing pathogen
Bacillus anthracis
2007-2011 290.00
21 Indian Council of
Medical Research
Characterization of DNA Gyrase from
Bacillus anthracis: A potential target
for new drugs
2008-2011 27.00
22 Department of
Biotechnology
Development of Mycobacterial
Antigen Delivery System using
Anthrax adenylatecyclase
2006-2010 78.43
23 Indian Council of
Medical Research
Production of recombinant vaccine
against anthrax using yeast expression
system
2006-2009 24.03
24 Council of
scientific
Industrial Research
Creation of Catalytically Inactive
variants of edema factor and lethal
factor from B. anthracis and its
Application in vaccine Development
against anthrax
2006-2009 14.00
25 Indian Council of
Medical Research
Development of Mycobacterial
Antigen Delivery System using
Anthrax Toxin components
2006-2009 20.00
26 Indian Council of
Medical Research
Therapeutic intervention of anthrax :
A molecular Medicine approach
2006-2009 23.44
27 Department of
Biotechnology
Development of DNA vaccine against
rabies using targeted vectors for
veterinary use (project with Dr. Anant
Rai, IVRI, Izatnagar)
2005-2009 68.21
28 Indian Council of
Agriculture Research
Search for eco-friendly bio-
insecticides from
Xenorhabdusnematophila and its
applications in aquaculture-a
molecular approach
2005-2008 43.83
29 Department of
Biotechnology
Programmed cell death in pathogenic
bacteria:- potential for developing
novel antibiotics to control infectious
diseases
2005-2008 51.66
30 Department of
Biotechnology
Generation of Non-toxic Lethal Factor
and Edema Factor for the
development of improved vaccine
against Anthrax.
2002-2005 35.09
31 Ministry of
Environment &Forest,
GOI
Search for Novel bioinsecticides from
Xenorhabdusnematophilus
2002-2005 17.93
32 UGC under
University
Of Excellence
Programme
New strategies to control Anthrax–
Mapping of the lethal factor binding
domain of Protective Antigen of
anthrax toxin.
2002-2005 15.00
33 NATP-World
Bank
Transgenic plants as a source of
edible vaccine against anthrax.
2000-2003 46.20
34 Department of
Biotechnology
Production of thermostable protective
antigen of Bacillus anthracis:
Computational and genetic
engineering approach.
1999-2002 18.71
35 Department of
Biotechnology
Generation of non-toxic Protective
antigen molecules for development of
recombinant anthrax vaccine.
1998-2002 45.20
36 Department of
Biotechnology
Cloning and Expression of non-toxic
variant of protective antigen for
developing recombinant vaccine
against anthrax.
1995-1999 55.00
37 DRDE Cloning and expression of lethal 1993-1995 12.0
factor gene of B. anthracis
38 DRDE Overexpression of protective antigen
gene of B. anthracis
1991-1993 8.0
39 Department of
Atomic Energy
Major Basic proteins from
Eisonophils
1995-1997 8.0
40 Indian Council of
Medical Research
Cloning and expression of dextran
sucrose gene from
leuconostocmesenteroides
1990-1993 5.0
GRAND TOTAL 882.679+2051.784 2934.463
PATENTS:
S.No.
Patent Title
Submitted
Pending
Award
Awarded
( INDIAN)
1 Generation and functional characterization of anti-Mtb Enolase monoclonal antibody Amit Rahi, Alisha Dhiman, Damini Singh,
Priyanka, Jaishree Garhyan, Rakesh Bhatnagar.(2017)
Submitted
2 Poly L-glutamines adjuvants isolated from Mycobacterium tuberculosis H37Rv cell wall. Manish Gupta, Rajesh Mani, Nirupama Banerjee,
Rakesh Bhatnagar, Ravi Tandon.(2017)
Submitted
3 A novel anthrax vaccine comprising LF-PA chimera Somya Aggarwal,
Vikas Kumar Somani, Sonal Gupta & Rakesh Bhatnagar (2017) Submitted
4 1,3,6-Trigalloyl-β-D-Glucose and Quercitin Dihydrate: The Novel and
Potential Therapeutic Compounds Against Anthrax. Vikas Kumar
Somani*1, Somya Aggarwal*1, Sukriti Goyal2, Abhinav Grover2 &
Rakesh Bhatnagar (2017)
Submitted
5 Hedgehog functionalized nanoparticles. Samer Singh, Rakesh Bhatnagar.
(2012) Submitted
6 A novel bispecific monoclonal antibody capable of cross reacting with
the lethal factor(LF) and edema factor(EF) and neutralizing edema toxin(ET) as well as lethal toxin(LT) of Bacillus anthracis. Parul
Kulshreshtha, Rakesh Bhatnagar(2010)
Submitted
7 A Pharmaceutical Composition of Vaccine against Rabies which Comprises of DNA Vaccine based on Glycoprotein Gene of Rabies
Virus Conjugated to LAMP-1 Signal Sequence of the C-Terminus and an
Adjuvant and Method of its Preparation thereof. Manpreet Kaur, Anant
Rai, Rakesh Bhatnagar (2008)
Submitted
8
DNA Vaccine against Anthrax. Shuchi Midha, Rakesh Bhatnagar. (2007)
Submitted
9 Insecticidal XnGroEL protein of xenorhabdus nematophila. Mohan
Chandra Joshi, Nirupama Bannerjee, Rakesh Bhatnagar (2007)
Submitted
10 A process for constitutively preparing anthrax protective antigen.
Waheed Mohsin, Vibha Chauhan, Rakesh Bhatnagar (2001)
Granted
11 An Anthrax Protective Antigen. Pankaj Gupta, Rakesh Bhatnagar (2002) Granted
12 Transformation of an Edible Crop with paga Gene of Bacillus anthracis.
M. A. Aziz, Rakesh Bhatnagar (2005)
Submitted
13 An immunodominant B cell epitope based protein vaccine against
Anthrax.Manpreet Kaur, Hema Chug, Rakesh Bhatnagar (2008).
Submitted
PCT
1 A Pharmaceutical Composition of Vaccine against Rabies which
Comprises of DNA Vaccine based on Glycoprotein Gene of Rabies
Virus Conjugated to LAMP-1 Signal Sequence of the C-Terminus and an Adjuvant and Method of its Preparation thereof. Manpreet Kaur, Anant
Rai, Rakesh Bhatnagar (2008)
Granted
2 DNA Vaccine against Anthrax. Shuchi Midha, Rakesh Bhatnagar.
(2008)
Submitted
3 Insecticidal XnGroEL protein of xenorhabdus nematophila. Mohan
Chandra Joshi, Nirupama Bannerjee, Rakesh Bhatnagar (2008)
Submitted
4 A Process for the Preparation of Non-Toxic Anthrax Vaccine. Vibha
Chauhan, Rakesh Bhatnagar (2001)
Granted
5 A process for constitutively preparing anthrax protective antigen.
Waheed Mohsin, Vibha Chauhan, Rakesh Bhatnagar (2001)
Granted
6 High Level Constitutive Production of Anthrax Protective Antigen.
Europe. Waheed Mohsin, Vibha Chauhan, Rakesh Bhatnagar
Granted
7 An immunodominant B cell epitope based protein vaccine against
Anthrax.Manpreet Kaur, Hema Chug, Rakesh Bhatnagar (2009).
Submitted
INTERNATIONAL
1 High Level Constitutive Production of Anthrax Protective Antigen.
Europe. Waheed Mohsin, Vibha Chauhan, Rakesh Bhatnagar. (US
Patent, 2001)
Granted
2 High Level Constitutive Production of Anthrax Protective Antigen.
USA. Waheed Mohsin, Vibha Chauhan, Rakesh Bhatnagar. (European
Patent, 2001)
Granted
3 A Process for the Preparation of Non-Toxic Anthrax Vaccine. USA.
Pankaj Gupta, Vibha Chauhan, Praveen Kumar, Nidhi Ahuja, Smriti
Batra, Aparna Singh, Rakesh Bhatnagar. (US Patent, 2002).
Granted
4 A Process for the Preparation of Non-Toxic Anthrax Vaccine. Europe.
Pankaj Gupta, Vibha Chauhan, Praveen Kumar, Nidhi Ahuja, Smriti
Batra, Aparna Singh, Rakesh Bhatnagar. (European Patent, 2002).
Granted
5 A Novel Bispecific Antibody capable of cross reacting into lethal factor
(LF) and edema factor (EF) and neutralizing edema toxin (ET) as well as
lethal toxin (LT) of Bacillus anthracis. USA. Parul Kulshrestha &
Rakesh Bhatnagar (US Patent, 2010)
Submitted
RESEARCH PUBLICATIONS
175. Gogoi, H., Mani, R., Aggarwal, S., Malik, A., Munde, M. and Bhatnagar, R. (2019)
Crystalline and amorphous preparation of aluminum hydroxide nanoparticles enhances
protective antigen domain 4 specific immunogenicity and provides protection against
anthrax Int. J. Nanomedicine. (Accepted)
174. Joshi, H., Malik, A., Aggarwal, S., Munde, M., Maitra, S. S., Adlakha, N. and
Bhatnagar, R. (2019) In-vitro detection of phytopathogenic fungal cell wall by
polyclonal sera raised against Trimethyl chitosan nanoparticles. Int. J. Nanomedicine.
(Accepted)
173. Garhyan, J, Das, B. and Bhatnagar, R. (2019). Mesenchymal stem cells: A hidden
arsenal for Mtb persistence, resuscitation and reactivation. In : Mycobacterium
tuberculosis: Molecular Infection Biology, Pathogenesis, Diagnostics and new
Interventions. Springer Nature Singapore Pte Ltd. (In Press)
172. Garg, R., Mani, R., Gupta, M., Tripathi, D., Chandra, H., Bhatnagar, R. and Banerjee,
N. (2019). Importance of cell wall-associated Poly-α-Lglutamine in the biology of
pathogenic mycobacteria. In: Mycobacterium tuberculosis: Molecular Infection Biology,
Pathogenesis, Diagnostics and new Interventions. Springer Nature Singapore Pte Ltd.
(In Press)
171. Mohan, S., Rangappa, S., Fuchs, J.E., Bender, A. and Bhatnagar, R. (2019). Sulfated
ceria catalyzed synthesis of imidazopyridines and their implementation as DNA minor
groove binders. Chem..biodivers. doi:10.1002/cbdv.201800435
170. Malik, A., Gupta, M., Mani, R. and Bhatnagar, R. (2019) Single dose Ag85B-ESAT-6
loaded PLGA nanoparticles confer protective immunity against tuberculosis. Int. J.
Nanomedicine 14: 3129-3143
169. Majumder, S., Das, S., Somani, V., Makam, S.S., Kingston, J.J. and Bhatnagar,
R.(2019). A bivalent protein r-PAbxpB comprising PA domain IV and exosporium
protein BxpB confers protection against B. anthracis spores and toxin
Front. Immunol. DOI: 10.3389/fimmu.2019.00498
168. Aggarwal, S., Somani, V., Gupta, S., Garg, R. and Bhatnagar, R., (2019) Development
of a novel multiepitope chimeric vaccine against anthrax. Med. Micro. Immunology.
DOI: 10.1007/s00430-019-00577-x
167. Kandari, D., Gopalani, M., Gupta, M., Joshi, H. and Bhatnagar, R. (2019) Identification,
Functional Characterization, and Regulon Prediction of the Zinc Uptake Regulator (zur)
of Bacillus anthracis – An Insight Into the Zinc Homeostasis of the Pathogen. Front.
Micorbiol. DOI: 10.3389/fmicb.2018.03314
166. Nayak, P.S., Boorah, S.M., Gogoi, H., Asthana, S., Bhatnagar, R., Jha, A.N. and Jha, S.
(2019) Lactoferrin adsorption onto silver nanoparticle interface: Implications of corona
on protein conformation, nanoparticle cytotoxicity and the formulation adjuvanticity.
J. Chem. Engg. DOI: 10.1016/j.cej.2018.12.084
165. Singh, D., Rahi, A., Kumari, R., Gupta, V., Gautam, G., Aggarwal, S., Rehan, M. and
Bhatnagar, R. (2019). Computational and mutational analysis of TatDDNase of Bacillus
anthracis. J. Cell Biochem. DOI: 10.1002/jcb.28408.
164. Mani R, Gupta M, Malik A, Tandon R, Prasad R, Bhatnagar R, Banerjee N.(2018)
Adjuvant Potential of Poly-α-L- glutamine from Cell Wall of Mycobacterium
tuberculosis. Infect Immun. Doi: 10.1128/IAI.00537-18.
163. Kant, N., Kulshreshtha, P., Singh, R., Mal, A., Dwivedi, A., Ahuja, R., Mehra, R.,
Tehlan, M., Ahmed, P., Kaushik, S., Shipra, Kumar, S., Mohammed, A., Shukla, S.,
Singh, D. and Bhatnagar, R. (2018). A study to identify the practices of the buffalo
keepers which inadvertently lead to the spread of brucellosis in Delhi. BMC Veterinary
Research 14(1) DOI: 10.1186/s12917-018-1670-2
162. Malik A.,Bhatnagar, R. (2018). Novel application of Trimethyl chitosan as an adjuvant
in vaccine delivery. Int. J. Nanomedicine 13 : 7959—7970.
161. Gogoi, H., Mani, R. and Bhatnagar, R. (2018). A niosome formulation modulates the
Th1/Th2 bias immune response in mice and also provides protection against anthrax
spore challenge .Int. J. Nanomedicine13: 7427–7440.
160. Majumder, S., Das, S., Somani, V., Makam, S.S., Kingston J. J., Bhatnagar, R. (2018) A
bivalent protein r-PB, comprising PA and Bcl Aimmunodominant regions for
comprehensive protection against Bacillus anthracis. Scientific Reports. 8:7242 .
159. Malik A., Gupta, M., Mani, R., Gogoi, H. and Bhatnagar, R. (2018). Trimethyl
chitosan nanoparticles encapsulated protective antigen protects the mice against anthrax.
Front. Immunol. 1(562): 1-12.
158. Gupta, V., Jain, K., Garg, R., Malik, A., Gulati, P. and Bhatnagar, R..(2018)
Characterization of a two component system, Bas1213-1214, important for oxidative
stress in Bacillus anthracis.J Cell Biochem., 1:14
157. Rahi, A., Dhiman, A., Singh, D., Lynn, A.M., Rehan, M., and Bhatnagar, R. (2018)
Exploring the interaction between Mycobacterium tuberculosis Enolase and human
plasminogen using computational methods and experimental techniques. J Cell Biochem.
119(2):2408-2417
156. Pasari, N., Adlakha, N., Gupta, M., Bashir, Z., Rajacharya, G.,Verma, G., Munde, M.,
Bhatnagar, R. and Yazdani S.S. (2017). Impact of Module-X2 and Carbohydrate
Binding Module-3 on the catalytic activity of associated glycoside hydrolases towards
plant biomass. Scientific reports. 16-43246C
155. Manish, M. and Bhatnagar, R. (2017) Antibody Response is Differentially Influenced
by PLGA-PAD4 Particle Characteristics. Int. J. Infact. 4(4):e15594.
154. Gayan, S., Mokhtari, R.B., Pal, B., Sarma, A., Talkundar, J., Sandhya, S., Bhuyan, R.,
Bhuyan, S., Garhyan, J., Baishya, D., Kataki, A., Bhatnagar, R.,Yeger, H. and das, B.
(2017). MYC and HIF-2alpha mediates resistance to Epidermal Growth Factor Receptor
(EGFR) antagonism in oral squamous carcinoma cells. Cancer Research 77(13):2056-
2056
153. Joon, S., Gopalani, M., Rahi, A., Kulshreshtha, P., Gogoi, H., Bhatnagar, S and
Bhatnagar, R.(2017) Biochemical characterization of the GTP sensing protein, CodY of
Bacillus anthracis. Pathogens and Disease., 75(4): 1-9
152. Gupta, V., Chaudhary, N., Aggarwal, S., Gulati, P. and Bhatnagar, R. (2017) Functional
analysis of BAS2108-2109 two component system: evidence for protease regulation in
Bacillus anthracis. Int. J. Biochem. Cell Biol. 89: 71-84
151. Garg, R., Kaur, M., Saxena, A., Prasad, R. and Bhatnagar, R. (2017). Alum adjuvanted
rabies DNA vaccine confers 80% protection against lethal 50 LD50 rabies challenge
virus standard strain. Mole.Immunol. 85:166-173.
150. Nagar, S.D., Aggarwal, B., Joon, S., Bhatnagar, R. and Bhatnagar, S. (2016). A
Network Biology Approach to Decipher Stress Response in Bacteria Using Escherichia
coli As a Model. Omics: a journal of integrative biology. 20 (5): 310-324.
149. Dhiman A, Rahi, A., Gopalani, M., Bajpai, S. Bhatnagar, S. and Bhatnagar, R. (2016).
Role of the Recognition Helix of Response Regulator WalR from Bacillus anthracis in
DNA Binding and Specificity. Int J Biol Macromol . 96:257-264
148. Panda, G. and Bhatnagar, R. (2016). Recent advances in anthrax pathophysiology,
prophylaxis and treatment ; Eds. A.Datta and V.P.Sharma, pp. 280-291.
147. Aggarwal, S., Somani, V.K., Gupta, V., Kaur, J., Singh, D., Graver, A. and Bhatnagar, R.
(2016). Functional Characteriazation of PhoPR two component system and its implication
in regulating phosphate homeostasis in bacillus anthracis. Biochim Biophys Acta
1861:2956-2970.
146. Rahi A, Matta SK, Dhiman A, Garhyan J, Gopalani M, Chandra S, Bhatnagar, R.(2016)
Enolase of Mycobacterium tuberculosis is a surface exposed plasminogen binding protein.
Biochim Biophys Acta.1861:3355-3364.
145 Gopalani M, Dhiman A, Rahi A, Kandari D, Bhatnagar R.(2016). Identification,
Functional Characterization and Regulon Prediction of a Novel Two Component System
Comprising BAS0540-BAS0541 of Bacillus anthracis. PLoSOne. 11(7):e0158895. doi:
10.1371/journal.pone.0158895.
144. Gupta M, Nayyar N, Chawla M, Sitaraman R, Bhatnagar, R. and Banerjee N (2016)
The Chromosomal parDE2 Toxin–Antitoxin System of Mycobacterium tuberculosis
H37Rv: Genetic and Functional Characterization. Front. Microbiol. 7:886. doi:
10.3389/fmicb.2016.00886.
143. Chaudhary N, Tøndel K, and Bhatnagar, R, Martins Dos Santos VA, Puchałka J. (2016).
Characterizing the optimal flux space of genome-scale metabolic reconstructions through
modified latin-hypercube sampling .Mol Biosyst. 23;12(3):994-1005.
142 Somani VK, Aggarwal S, Singh D, Prasad T, and Bhatnagar R. (2016). Identification of
novel raft marker protein, FlotP in Bacillus anthracis. Front. Microbiol. 169(7): 1-18.
141. Newly identified motifs in Candida albicans Cdr1 protein nucleotide binding domains
are pleiotropic drug resistance subfamily-specific and functionally asymmetric (2016)
Manpreet Rawal, Atanu Banerjee, Abdul Shah, Mohammad Firoz Khan, Sobhan Sen,
Ajay Kumar Saxena, Brian Monk, Richard D. Cannon, Rakesh Bhatnagar, Alok Kumar
Mondal, and Rajendra Prasad. Scientific Reports. 6:27132
140. Manish M, Bhatnagar R and Singh S. (2016). Preparation and Characterization of
PLGA Encapsulated Protective Antigen Domain 4 Nanofor mulation. Methods. Mol
Biol. 1404:669-81.
139. Garhyan J, Bhuyan S, Pulu I, Kalita D, Das B and Bhatnagar R. (2015). Preclinical and
clinical evidence of Mycobacterium tuberculosis persistence in the hypoxic niche of bone
marrow mesenchymal stem cells after therapy.Am J Pathol.185(7): 1924-34.
138. Panda, G, Tanwer, P, Ansari, S, Khare, D. and Bhatnagar, R. (2015). Regulation and
RNA binding properties of Hfq-like RNA chaperones in Bacillus anthracis, Biochimic
Biophysic Acta. 1850(9): 1661-8.
137. Aggarwal, S, Somani VK, Bhatnagar, R. (2015). Phosphate starvation enhances the
pathogenesis of Bacillus anthracis. Int. J. Med. Microbiol. 305(6): 523-31.
136. Singh, D, Goel D, Bhatnagar R.(2015).Recombinant L7/L12 protein entrapping PLGA
(Poly lactide-co-glycolide) micro particles protect BALB/c mice against the virulent B.
abortus 544 infection. Vaccine. 33(24): 2786-92.
135. Singh D, Somani VK, Aggarwal S, Bhatnagar, R.. (2015). PLGA (85:15) nanoparticle
based delivery of rL7/L12 ribosomal protein in mice protects against Brucellaabortus 544
infection: A promising alternate to traditional adjuvants. Mol. Immunol. 68(2):272-279.
134. Kulshreshtha P, Tiwari A, Priyanka, Joon S, Sinha S, Bhatnagar R. (2015). Investigation
of a panel of monoclonal antibodies and polyclonal sera against anthrax toxins resulted in
identification of an anti-lethal factor antibody with disease-enhancing characteristics.Mol.
Immunol. 68(2):185-193.
133. Gopalani M, Dhiman A, Rahi A, Bhatnagar R. (2015).Overexpression of the Pleiotropic
Regulator CodY Decreases Sporulation, Attachment and Pellicle Formation in Bacillus
anthracis.Biochem. Biophys Res Commun. 469 (3):672-8
132. Dhiman A, Gopalani M, Bhatnagar R. (2015). WalRK two component system of Bacillus
anthracis responds to temperature and antibiotic stress.Biochem. Biophys Res
Commun;459 (4);623-8.
131. Tripathi D, Kant S, Garg R, Bhatnagar R (2015).Low expression level of glnA1 accounts
for absence of cell wall associated poly-L-glutamate/glutamine in Mycobacterium
smegmatis. Biochem Biophys Res Commun458(2);240-5.
130 Aggarwal S., Somani,V. K. and Bhatnagar, R. (2015).Phosphate starvation enhances the
pathogenesis of Bacillus anthracis Int. J. Med. Microbiol. 305(6):523-31.
129. Chaudhary P, SinghS, Bhatnagar R, Sarin NB and Farhat S.(2014). Basic Expression of
Edema Factor Gene (cya) from Bacillus anthracis in Model Plant Tobacco .P.1143-1148.
128. Kaur M, Garg R, Singh S, Bhatnagar R. (2014). Rabies vaccines: where do we stand,
where are we heading? Expert Rev Vaccines;28: 1-13.
127. Garg R, Tripathi D, KantS, Chandra H, Bhatnagar R, Banerjee
N.(2014).Conserved Hypothetical Protein Rv0574c Is Required for Cell Wall Integrity,
Stress Tolerance and Virulence of Mycobacterium tuberculosis. Infect Immun83(1): 120–
129
126. Verma S, Bhatnagar R. (2014). MoxT toxin of Bacillus anthracis exhibits sequence
specific ribonuclease activity Biochem Biophys Res Commun450(2);998-1004.
125. Garg R, Kaur M, Bhatnagar R. (2014). DNA vaccination for Rabies: Evaluation of
Preclinical Safety and Toxicology. Trials in Vaccinology;3: 73–80
124. Verma S, Kumar S,Gupta VP, Gourinath, S, Bhatnagar S, Bhatnagar R. (2014) Structural
basis of Bacillus anthracisMoxXT disruption and the modulation of MoxTribonuclease
activity by rationally designed peptides. J. Biomol. Struct.Dyn33(3);606-24.
123. Gorantala J, Grover S, Rahi A, Chaudhary P, Rajwanshi R, Sarin NB, Bhatnagar R.
(2014). Generation of Protective immune response against anthrax by oral immunization
with protective antigen plant based vaccine. J. Biotechnol.176:1-10.
122. Dhiman, A, Bhatnagar S, KulshreshthaP,Bhatnagar, R. (2014). Functional
Characterization of WalRK: a Two Component Signal Transduction System from Bacillus
anthracis.FEBS Open Bio4: 65–76.
121 .Panda, G, Basak, T, Tanwer, P, Sengupta, S, Vitor, A.P. M Santos,Bhatnagar, R.
(2014).Delineating the Effect of Host Environmental Signals on a Fully Virulent Strain of
Bacillus anthracis usingan Integrated Transcriptomics and Proteomics Approach.J.
Proteom.105;242-65.
120. Chopra, N, Pathak,S.A.andBhatnagar, S.Bhatnagar, R. (2013).Linkage, Mobility and
Selfishness in the MazF Family of Bacterial Toxins: A Snapshot of Bacterial Evolution.
Genome Biol. Evol5(12): 2268-2284.
119. Kaur M, Singh S, Bhatnagar R. (2013). Anthrax vaccines: present status and future
prospects.Expert Rev Vaccines12(8):955-70.
118. Tripathi D, Chandra H,Bhatnagar R.(2013).Poly-L-glutamate/glutamine synthesis in the
cell wall of Mycobacterium bovis is regulated in response to nitrogen availability. BMC
Microbiology; 13: 226.
117. ManishM, RahiA, KaurM, SinghS.BhatnagarR.(2013).A Single-Dose PLGA
Encapsulated Protective Antigen Domain 4 Nanoformulation Protects Mice against
Bacillus anthracis Spore Challenge. PloS One; 8 (4): e61885
116. Goel D, Rajendran V, Ghosh PC andBhatnagar R. (2013). Cell mediated immune
response after challenge in Omp25 liposome immunized mice contributes to protection
against virulent Brucellaabortus 544. Vaccine; 31 (8): 1231-1237
115. Sinha K and Bhatnagar, R. (2013). Recombinant GroEL enhances protective antigen-
mediated protection against Bacillus anthracis spore challenge. Medical Microbiology
and Immunology; 202(2): 153-65
114. Goel D andBhatnagar R. (2012). Intradermal immunization with outer membrane
protein 25 protects Balb/c mice from virulent B. abortus 544. MolImmunol.51(2):159-68
113. Kulshreshtha, P,Aggarwal,S., Jaiswal,H. andBhatnagar, R. (2012) S-layer homology
motif is an immunogen and confers protection to mouse model against anthrax. Mol.
Immunol.50(1-2):18-25.
112. Kaur M., Bhatnagar R. (2011). Recent Progress in development of anthrax
vaccines.Recent Patents on Biotechnology5(3):148-159.
111. Gorantala J, Grover S, Goel D, Rahi A, Chandra S, Bhatnagar R. (2011). A plant based
protective antigen [PA(dIV)] vaccine expressed in chloroplasts demonstrates protective
immunity in mice against anthrax. Vaccine; 29(27):4521-33.
110. Rahi A., Rehan M., Garg R, Tripathi D., Lynn A.M., and Bhatnagar
R. (2011). Enzymatic characterization of Catalase from Bacillus anthracis and prediction
of critical residues using information theoretic measure of Relative
Entropy. Biochem.Biophys. Res. Commun.; 411(1):88-95.
109. Kulshreshtha P. and Bhatnagar R. (2011). Inhibition of anthrax toxins with a bispecific
monoclonal antibody that cross reacts with edema factor as well as lethal factor
of Bacillusanthracis. Mol.Immunol.48(15-16):1958-65.
108. Chopra, N., Agarwal, S., Verma, S., Bhatnagar, S., Bhatnagar R. (2011).Modeling of the
structure and interactions of the B. anthracis antitoxin, MoxX: deletion mutant studies
highlight its modular structure and repressor function. J Comput Aided Mol Des; 25:275–
291.
107. Sinha, K. andBhatnagar,R.. (2010). GroEL provides protection against Bacillus
anthracis infection in BALB/c mice Mol.Immunol. 48: 264-71
106. Matta S.K, Agarwal S, Bhatnagar R (2010).Surface localized and extracellular
Glyceraldehyde-3- phosphate dehydrogenase of Bacillus anthracis is a plasminogen
binding protein. Biochim.BiophysActa.; 1804(11):2111-2120
105. Agarwal S, Mishra N.K, Bhatnagar S. and Bhatnagar R. (2010).PemK Toxin of Bacillus
anthracisis a Ribonucleasean insight into its active site, structure and function
J.Biol.Chem;285:7254-7270.
104. Kaur M, Saxena A, Rai A. and BhatnagarR. (2010). Rabies DNA vaccine encoding
lysosome targeted glycoprotein supplemented with EMULSIGEN D confers complete
protection in pre-and post-exposure studies in BALB/c mice. FASEB J; 24:173-183
.
103. Chawla A. and Bhatnagar R. (2009). AnthraxVaccine.Adult Immunization; 1: 1-7.
102. Chawla A., Midha S. and BhatnagarR. (2009). Efficacy of recombinant anthrax vaccine
against Bacillus anthracis aerosol spore challenge: Pre-clinical evaluation in Rabbits and
Rhesus monkeys. Biotechnology Journal;4: 1-9.
101. Kaur M., Chug H., Singh H., Chandra S., Mishra M., Sharma M. and Bhatnagar
R.(2009). Identification And Characterization of Immunodominant B-Cell Epitope of The
C-Terminus of Protective Antigen of Bacillus anthracis. Mol.Immunol. 46:2107-2115.
100. Kaur M., Rai A. and Bhatnagar R. (2009). Rabies DNA vaccine: No impact of MHC
Class I and Class II targeting sequences on immune response and protection against lethal
challenge. Vaccine;27: 2128-2137.
99. Midha S. and Bhatnagar R. (2009). Genetic Immunization with GPI-anchored Anthrax
Protective antigen raises combined CD1d- and MHC II-restricted Antibody Responses by
Natural Killer T cell-mediated help. Vaccine; 27(11):1700-9
98. Midha S. and Bhatnagar R. (2009). Anthrax protective antigen administered by DNA
vaccination to distinct subcellular locations potentiates humoral and cellular immune
responses. European Journal of Immunology;39(1):159-77
97. Kanodia S., Agarwal S., Singh P., Agarwal S., Singh P., Bhatnagar R. (2009).
Biochemical and functional characterization of alanine racemase spore protein of Bacillus
anthracis. Biochem.Mol.Biol. Reports;42(1): 47-52.
96. Joshi M.C., Sharma A., Kant S., Birah A., Gupta G.P., Khan S.R., Bhatnagar R. and
Banerjee N. (2008). An Insecticidal GroEL protein with chitin binding activity from
Xenorhabdusnematophila.J.Biol.Chem.;283(42):28287-96.
95. Agarwal S., Kulshrestha P., Mukku D.B. and Bhatnagar R. (2008).-enolase binds to
human plasminogen on the surface of Bacillus anthracis. Biochim.BiophysActa.;
1784:986-94
94. Gupta M., Alam S. and Bhatnagar R. (2007). Catalytically inactive anthrax toxin(s) are
potential prophylactic agents. Vaccine;25: 8410-8419.
93. Chandra S, Kaur M, Midha S, Gorantala J. and Bhatnagar R. (2007). Induction of
cytotoxic T lymphocyte response against Mycobacterial antigen using domain I of anthrax
edema factor as antigen delivery system. Biochem.Biophys. Res. Commun;357:50-55.
92. Aziz M. A., Midha S., Waheed S. M. and Bhatnagar R. (2007). Oral vaccines: New
needs, New Possibilities. BioEssays;29: 591-604.
91. Agarwal S., Agarwal S. and Bhatnagar R. (2007). Identification and Characterization of a
Novel Toxin-Antitoxin Module from Bacillus anthracis.FEBS Letters; 581: 1727-34.
90. Chandra S., Kaur M., Midha S., Bhatnagar N. B. and Bhatnagar R. (2006).Evaluation of
the ability of N-terminal fragment of lethal factor of Bacillus anthracis for delivery of
Mycobacterium T-cell antigen ESAT-6 into cytosol of antigen presenting cells to elicit
effective cytotoxic Tlymphocytes response. Biochem.Biophys. Res. Commun; 351: 702-
7.
89. Gupta M., Alam S. and Bhatnagar R. (2006). Kinetic characterization and ligand binding
studies of His351 mutants of Bacillus anthracis denylatecyclase. Archives of
Biochemistry and Biophysics; 446:28-34.
88. Alam S., Gupta M. and Bhatnagar R. (2006).Inhibition of platelet aggregation by anthrax
edema toxin. Biochem.Biophys. Res. Commun.; 399:107-114.
87. Midha S., Mishra R., Aziz M. A., Sharma M., Mishra A., Khandelwal P. and Bhatnagar
R. (2005). Cloning, Expression and Characterization of Recombinant Nitric Oxide
Synthase-like protein from Bacillus anthracis.Biochem.Biophys. Res. Commun; 336:
346-356
86. Aziz M. A., Sikriwal D., Singh S., Jarugula S., Kumar A. and Bhatnagar R. (2005).
Transformation of an edible crop with pagAgene of Bacillus anthracis.FASEB J;19:1501-
3
85. Singh S., Singh A., Aziz M. A., Waheed S. M., Bhat R. and Bhatnagar R.
(2004).Thermalinactivation of Protective Antigen of B. anthracis and its prevention by
polyosmolytes.Biochem.Biophys. Res. Commun;322:1029-1037.
84. Ahuja N, Kumar P. and Bhatnagar R. (2004). Adenylatecyclase toxins.Crit. Rev.
Microbiol.; 30:187–196.
83. Singh J, Joshi M.C. and Bhatnagar R. (2004).Cloning and expression of mycobacterial
glutamine synthetase gene in E. coli. Biochem.Biophys. Res. Commun.; 317:634-638.
82. Singh S, Aziz M. A, Khandelwal P, Bhat R. and Bhatnagar R. (2004). The
osmoprotectants glycine and its methyl derivatives prevent the thermal inactivation of
protective antigen of Bacillus anthracis. Biochem.Biophys. Res. Commun.; 316: 559–
564.
81. Khandelwal P, Bhatnagar R, Choudhury D. and Banerjee N. (2004). Characterization of
a cytotoxic pilin subunit of Xenorhabdusnematophila.Biochem.Biophys. Res. Commun.;
314:943-949.
80. Chattopadhyay A, Bhatnagar R. and Bhatnagar N. (2004). Bacterial Insecticidal Toxins
Crit.Rev. Microbiol.;30:33–54.
79. Ahuja N, Kumar P, Alam S., Gupta M. and Bhatnagar R. (2003).Deletion mutants of
protective antigen that inhibit anthrax toxin both in vitro and in vivoBiochem. Biophys.
Res. Commun.; 307: 446–450.
78. Aziz M.A, Singh S, Kumar A. P. and Bhatnagar R. (2002). Expression of protective
antigen in transgenic plants: a step towards making edible vaccine against anthrax.
Biochem.Biophys. Res. Commun.; 299:345-51.
77. Kumar P, Ahuja N. and Bhatnagar R. (2002). Anthrax edema toxin requires influx of
calcium for inducing cAMP toxicity in target cells. Infect. Immun.; 70: 4997-5007.
76. Singh S, Ahuja N, Chauhan V, Rajasekaran E, Waheed S.M, Bhat R. and Bhatnagar R.
(2002). Gln 277 and Phe 554 residues are involved in thermal inactivation of protective
antigen of Bacillus anthracis.Biochem. Biophys. Res. Commun;296:1058-1062.
75. Chauhan V. and Bhatnagar R. (2002). Identification of amino acid residues of Protective
antigen involved in binding to Lethal factor Infect.Immun; 70:4477- 4484.
74. Singh A, Chauhan V, Sodhi A. and Bhatnagar R. (2002).Asp 187 and Phe 190 residues
in lethal factor are required for the expression of anthrax lethal toxin activityFEMS
Microbiology Letters; 212:183-6.
73. Singh A, Singh S, Waheed S. M, Khandelwal P. and Bhatnagar R. (2002). Expression of
anthrax lethal factor gene by osmolyte induction FEMS Microbiology Letters; 209: 301-
5.
72. Ahuja N, Kumar P. and Bhatngar R. (2001). Hydrophobic residues Phe552, Phe554,
Ile562, Leu566 and Ile574 are required for oligomerization of anthrax protective antigen.
Biochem.Biophy. Res. Commum;287: 542-9.
71. Ahuja N, Kumar P. and Bhatnagar R. (2001). Rapid Purification of Recombinant
Anthrax-Protective Antigen under Non-denaturing Conditions. Biochem.Biophys. Res.
Commun.; 286:6-11.
70. Batra S. and Bhatnagar R (2001). Anthrax Toxin.Crit. Rev. Microbiol.; 27: 167-200.
69. Gupta P, Sahai V. and Bhatnagar R. (2001). Enhanced expression of the recombinant
lethal factor of Bacillus anthracis by Fed-Batch culture.Biochem.Biophys. Res.
Commun;285: 1025-1033.
68. Gupta P, Singh S, Tiwari A, Bhat R. and Bhatnagar R. (2001). Effect of pH on stability
of Anthrax lethal factor: Correlation between denaturation and activity. Biochem.Biophy.
Res. Commun; 284:568-73.
67. Chauhan V, Singh A, Waheed S. M, Singh S. and BhatnagarR.(2001). Constitutive
Expression of Protective Antigen Gene of Bacillus anthracis in Escherichia
coli.Biochem.Biophy. Res. Commun; 283: 308-315.
66. Kumar P, Ahuja N. and Bhatnagar R. (2001). Purification of Anthrax Edema Factor from
Escherichia coli and identification of residues required for binding to Protective Antigen
Infec. & Immun;69:6532-6.
65. Batra S, Gupta P, Chauhan V, Singh A. and Bhatnagar R. (2001).Trp 346 and Leu 352
residues in protective antigen are required for the expression of anthrax lethal toxin
activity. Biochem.Biophy. Res. Commun;281:186-192.
64. Gupta P., Singh A, Chauhan V, and Bhatnagar R. (2001).Involvement of residues
147VYYEIGK153 in binding of Lethal factor to Protective antigen of Bacillusanthracis.
Biochem.Biophy. Res. Commun; 280: 158-163.
63. Adhuna., Salotra P. and Bhatnagar R. (2000). Nitric oxide induced expression of stress
proteins in virulent and avirulentpromastigotes of L.donovani.ImmunologyLetters;71:71-
176.
62. Gupta P, Waheed S.M. and Bhatnagar R. (1999). Expression and purification of the
recombinant Protective antigen of Bacillusanthracis.Protein
ExpressionandPurification;16: 369-376.
61. Bhatnagar R,Ahuja N, Goila R, Khetarpal S, Waheed S.M. and Gupta P. (1999).
Activation of phospholipase C and protein kinase C is required for expression of anthrax
lethal toxin cytotoxicity in J774A. 1 cells.CellularSignalling; 11: 111-116.
60. Gupta P, Khetarpal S, Chopra A.P, Singh Y, Bhatnagar R. (1998). Expression and
Purification of the recombinant Lethal factor of Bacillusanthracis.InfecImmun; 66:862-
865.
59. Bhatnagar R, Dixit A. and Roy K.B. (1998).Biochemistry(Book) National Open
School.
58. Salotra, P and Bhatnagar,R. (1998). Role of stress proteins in Leishmania.Chapter in
book “Microbes: For Health, Wealth & Sustainable Environment”. Malhotra Publishing
House, New Delhi. PP. 595-615
57. Adhuna, Salotra P, Mukhopadhya B. and Bhatnagar R. (1997). Modulation of
macrophage heat shock protein (HSPs) expression in response to intracellular infection by
virulent and avirulent strains ofLeishmaniadonovani.Biochem. Mol.Bio.Int.; 43: 1265-
1275.
56. Radha C, Salotra P, Bhat R. and Bhatnagar R. (1996). Thermostabilization of Protective
antigen-the binding component of anthrax lethal toxin.J.Biotechnol; 50: 235-242.
55. Salotra P, Seal K.P, Krishna N, Jaffe H. and Bhatnagar R. (1995). Expression of Dnak
and GroEl homologs in Leuconostocmesenteroides in response to heat shock or chemical
stress.FEMSMicrobiologyLetters; 131:7-62.
54. Salotra P, Chauhan D, Rallhan R. and Bhatnagar R. (1995). Tumor necrosis factor-alpha
induces preferential expression of stress proteins in virulent promastigotes of
Leshmaniadonovani. ImmunologyLetters; 44: 1-5.
53. Richa S, Salotra P, Bhatnagar R. and Datta K. (1995). L-alanine- dioxovalerate
transaminase in Leishmaniadonovani that differs from the mammalian
enzyme.Microbiol.Res.; 150: 1-5.
52. Salotra P, Ralhan R. and Bhatnagar R. (1994). Differential expression of heat shock
proteins in virulent and attenuated strains of
Leishmaniadonovani.BiochemMolecularBiologyInternational;33: 691-697.
51. Bhatnagar R. and Friedlander A.M. (1994). Protein synthesis is required for the
expression nthrax lethal toxin cytotoxicity.Infec.Immunol;62: 2958-62.
50. Friedlander A.M, Bhatnagar R,Leppla S.H, Johnson L. and Singh Y.
(1993).Characterization of macrophage sensitivity and resistance to anthrax lethal
toxin.Infec.lmmunol. 61: 245-252.
49. Dhaunsi G. S, Sidhu G.S, GargU.C, Singh Y, Chakravarti R.N. and Bhatnagar R. (1990).
Effect on intestinal nutrient uptake and brush border membrane enzymes in response to
atherogenic diet in rhesus monkeys.J. Med.Primatol;18: 467-475.
48. Dhaunsi G.S, Garg U.C,Ganguly N.K. and ChakravartiR.andBhatnagar R. (1990). Renal
brush border membrane enzymatic status in atherogenic diet fed Rhesus monkeys.
BiochemicalArchives;6: 95-102.
47. Leppla S.H, Friedlander A.M, Singh Y, Cora E.M. and Bhatnagar R. (1990).A model for
anthrax lethal toxin action at the cellular level.SalisburyMedicalBullatin; 68: 41-43.
46. Garg U.C, Ganguly N.K, Sharma S. and Bhatnagar R. (1990).Humoral and cell mediated
immunity in pyelonephritic rats immunized with fimbriae of Escherichia coli. Med. Sci.
Res; 18: 601-603.
45. Singh Y, LepplaS.H, Bhatnagar R. and Friedlander A.M. (1990). Basis of cellular
sensitivity and resistance to anthrax lethal toxin.Salisbury MedicalBullatin; 68: 46-48.
44. Singh Y, Leppla S.H, Friedlander A. andBhatnagar R. (1989). Internalization and
processing of Bacillus anthracis lethal toxin-sensitive and resistance cells.J.Biol.
Chem;254: 11099-11102.
43. Singh Y, Leppla S. and Friedlander A. and Bhatnagar R. (1989). Calcium is required for
expression of anthrax lethal toxin activity. Infec.Lmmun; 57: 2107-2114.
42. Krishna G, Singh Y, Sidhu G.S. and Bhatnagar R. (1989). Mechanism of MPTP induced
Parkinson's disease at cellular and subcellular level. In Neurochemical
Pharmacology165-174.
41. Singh Y, Bhatnagar R,Sidhu G.S, Batra J.K. and Krishna G. (1989). MPTP inhibits
proton motive force in energized mitochondria. Arch.Biochem.Biophys; 271: 217-222.
40. Garg U.C, Ganguly N.K, Sharma S. and BhatnagarR.(1989). Transport of nutrients into
the renal brush border membrane vesicles as markers in evaluating the role of antibodies
in modulation of ascending pyelonephritis.FEMSMicrobiol.Lett; 57: 155-160.
39. Mereish K.A, Solow R, Singh Y. and Bhatnagar, R. (1989). Comparative toxicity of
cyclic peptides.Med.Sci.Res;17: 869-871.
38. Mauviel A, Teyton L, Penfornis H, Laurent M, Hartman D, Bonaventure J, Loyau G,
Saklatvala J. and Pujol J.P. andBhatnagar R. (1988). Interleukin-I modulates collagen
gene expression in cultured synovial cells. Biochem.J; 252: 247-255.
37. Garg U.C, Rai N, Dhaunsi G.S, Sidhu G.S, Singh Y, Ganguly N.K. and Bhatnagar R.
(1988). A spectrophotometric assay for calmodulin.Biotechniques; 6: 294-296.
36. Falah AMS, Bhatnagar N, Singh Y, Murthy P.S. and Venkitasubramanian T.A. and
Bhatnagar R. (1988).On the presence of calmodulin like protein in Mycobacteria
FEMSMicrobiol.Let; 56: 89-94.
35. Singh Y, Chaudhary V.K, and Misra U.K. and Bhatnagar R. (1988). Mixed function
oxidases in response to quality and quality of dietary protein. Biochem.
International;17:1-8.
34. Singh Y, and MisraU.K.andBhatnagar R. (1988). Hepatic phosphatidylcholine synthesis
in defficiency of lysine and lysine and threonine: Effect of malathione. Experientia; 44:
758-759.
33. Garg U.C, Ganguly N.K. and Bhatnagar R. (1987). Brush border enzyme as markers for
ascending pyelonephritis: active immunization with pilli. FEMSMicrobiol Lett;43: 127-
131.
32. Garg U.C, Ganguly N.K, Sharma S. and Bhatnagar R. (1987).New sensitive markers for
the detection of experimental ascending pyelonephritis.LifeSci;41: 1-56.
31. Garg U.C, Ganguly N.K, Sharma S, Chakravarti R.N. and Bhatnagar R.
(1987).Quantitative histopathological method for the evaluation of experimental
pyelonephritis in rat.Med. Sci.Res;15:367-368.
30. Garg U.C, Ganguly N.K. and Bhatnagar R. (1987).Towards the mechanism of altered
nutrients uptake in brush border membrane vesicles from pyelonpehritis
rats.Biochem.In;14: 949-955.
29. Garg U.C, Ganguly N.K. and Bhatnagar R. (1987).Calmodulin: Factor contributing
towards altered nutrient uptake in pyelonephritis in rat. Med. Sci.Res; 15: 369-370.
28. Garg U.C, Ganguly N.K, Sharma S. and Bhatnagar R. (1987). Antipilli antibodies afford
protection against ascending pyelonephritis in rats: evaluation by renal brush border
membrane enzymes.Biochem.Int;14:517-524.
27. Garg U.C, Sharma D.K, Dhaunsi G.S. and Bhatnagar R. (1987). Altered intestinal brush
border membrane enzymes and nutrients uptake in naloxone treated rats Med.SciRes;15:
551-552.
26. Gill N.J, Garg U.C, Ganguly N.K, Mahajan R.C. and Bhatnagar R. (1987). Alteration of
brush border membrane enzymes in intestinal amoebiasis.Med. Sci. Res; 15: 101-102.
25. Garg U.C, Ganguly N.K, Sharma S. and Bhatnagar R. (1987).Prevention of alteration in
the uptake of nutrients in pyelonephritis immunization with pilli.Biochem.Int; 15:881-
886.
24. Gill N.J, Garg U.C, Ganguly N.Y, Mahajan R.C. and Bhatnagar R. (1987). Release of
lysosomal enzymes by kupffer cells in experimental hepatic amoebiasisMed. Sci. Res;15:
991-992.
23. Dhaunsi G.S, Garg U.C, Sharma D.K, Aulakh B.S. and Bhatnagar R. (1987). Towards
the mechanism of protection against indomethace in induced gastrointestinal ulceration by
naloxone. Med. Sci. Res;15: 1034-1035.
22. Dilwari J.B, Kumar A.V.K, Khurana S, and Dash R.J.andBhatnagar R.(1987).The effect
of legumes on blood glucose in diabetesmelitus.Ind.J.Med. Res;85:184-187.
21. Bhatnagar R,Penfornis H, Mauviel A., Loyau G, Saklatvala J. and Poujol J. P. (1986).
Interleukin-I inhibits and synthesis of collagen by fibroblast. Biochem.Int;13: 709-720.
20. Sidhu G.S, Dhuansi G.S, Garg U.C, Ganguly N.K, Chakravarti R.N. and Bhatnagar R.
(1986). Renal nutrients uptake and brush border membrane enzymes in cholesterol fed
guinea pigs. Biochem.Med;36:179-185.
19. Sidhu G.S, Garg U.C, Dhaunsi G.S, Bhari S.K. and Bhatnagar R. (1986). Enzymatic and
transport studies in cholesterol fed guinea pigs using intestinal brush border membrane
vesicle. Digestion; 34: 251-258.
18. Garg U.C, Ganguly N.K, Sharma S. and Bhatnagar R. (1986).Kinetics of nutrient uptake
in renal brush border membrane vesicles from rat with experimental
pyelonephritis.Biochem.Int; 7-14.
17. Garg U.C, Sidhu G.S, Sharma S, Chakravarti R.N, Ganguly N.K. and Bhatnagar R.
(1986). The presence of calmodulin in renal micrivillar core.Int. Res.Comm. Sys. Med.
Sci;14: 752-753.
16. Garg U.C, Sidhu G.S, Sharma S, Chakravarti R.N. and Bhatnagar R. (1985).
Pyelonephritis alters the reabsorption of nutrients and brush border enzymes of rat kidney
Biochem. Int;11:145-152.
15. Dhaunsi G.S, Garg U.C, Sidhu G.S. and Bhatnagar R. (1985). Enzymatic andtransport
studies on rat intestinal brush border membrane vesicles bound to pea and lentil lectins.
Int.Res.Comm.Sys. Med. Sci; 13: 469-70.
14. Bhatnagar N. and PrabhuK.A.andBhatnagarR. (1985). Extracellular dextransucrase from
a new strain of Leuconostocmesenteroides.Int. Res. Comm. Sys. Med. Sci;13: 129-130.
13. Bhatnagar N, and VenkitasubramanianT.A.andBhatnagar R. (1984).Characterization and
metabolism of cGMP in Mycobacterium smegmatis.Biochem. Biophys.Res.Commun;
121: 634-640.
12. Decker K, Birmelin M, and Rieder H.and Bhatnagar R. (1982)."Role of Ca++ and
prostaglandins in response of kuffer cells to external stimuli".InSinusoidalLiverCells(Ed.
E. Wisse and D.L. Knook) Elsevier Biomed.Press Holland; pp 367-379.
11. BhatnagarR, Schade U, Rietschel E.T. and Decker, K. (1982).Involvement of
prostaglandin E and cAMP in lipopolysaccride stimulated collagenase release in rat
kupffer cells. Eur. J.Bioche;125: 125-130.
10. Bhatnagar R. and Decker K. (1981). Collagenase assay using 3H- methylcollagen.
J.Biochem.Biophys.Methods 5: 147-152.
9. BhatnagarR, Schiermer R., Ernst M. and Decker K. (1981).Superoxide release by
zymosan stimulated kupffer cells in vitro. Eur.J.Biochem; 119: 171-175.
8. Banerjee N, Bhatnagar R. and Viswanathan L. (1981). Inhibitionn of glycolysis by
furfural in Saccharomyces cerevisiae.Eur.J.Microbiol Biotech; 11: 226-228.
7. Banerjee N, Bhatnagar R. and Viswanathan L. (1981). Development of resistance in
Saccharomyces cerevisiae against inhibitory effects of Browning reaction products
Enz.Microb.Tech; 3: 24-28.
6. BhatnagarR, Banerjee N, Srivastava H.C, and Prabhu K.A. (1981). Structure of dextran
synthesized by a new strain of Leuconostocmesenteroides.Carbohy;Res.89: 346-349.
5. Srivastava G.S, Bhatnagar R.,Viswanathan R. and Venkitasubramanian T.A.(1980).
Microsomal and mitrochondrial cytochromes in acutely hypoxic rat liver and lung
Ind.J.Biochem.Biophys;17: 130-134.
4. Banerjee N, Bhatnagar R. and Viswanathan L. (1980). Inhibition of growth and lactic
acid synthesis by maltol in Lactobacillus casie and its reversal by glutamic acid
Experentia; 36:313-314.
3 .Bhatnagar R. and Prabhu K.A. (1976). Studies on the removal of dextran by
dextranaseProc.Sug.Tech.Assoc;42: 101-106
2. Bhatnagar R. and Prabhu K.A. (1975). Effect of disinfectants on dextran forming
Leuconostoc bacteria. Proc. Sug. Tech. Assoc;41: 95-99.
1. Bhatnagar R. and Prabhu K.A. (1974). Studies on the use of cane waste molasses in the
production of dextran. Proc.Sug. Tech.Assoc;40: 23-27.
Prof. Rakesh Bhatnagar
