S13Abstracts / Cell Biology International 32 (2008) S1eS67

PRETREATMENT WITH BCG ATTENUATE CARDIACREMODELING VIA ACTIVATION OF TLR4Wen Feng Cai, Xiao Wei Zhang, Hui Min Yan, Qing Qing Wang,Liu Yuying, Bing Mu Xing, Han Zhi Liu, Hong Zhen Yang, ZhuoWei Hu*

Institue of Meteria Medica, Chinese Academy of Medical Sciencesand Peking Union Medical College, Beijing, ChinaHypertension-induced cardiovascular hypertrophy and fibrosis are critical in

the development of heart failure. We wondered if Bacillus Calmette Guerin

(BCG), a safe vaccine which has strong immune modulating effect, could pre-

vent cardiac hypertrophy and fibrosis in mice by regulation of immune micro-

enviroment through Toll like receptor 4 (TLR4) and DC-SIGN. Animals

received BCG intraperitoneally with or without TLR4 antagonist (msbB) or

DC-SIGN inhibitor (mannan) every 3 days for a week before abdominal aortic

constriction (AAC). Myocardial hypertrophy and fibrosis were evaluated by

echocardiography and pathohistology. Biochemical and immunological

changes were investigated by RT-PCR, immunohistochemistry and confocal.

Pretreatment of BCG attenuated myocardial hypertrophy and fibrosis, which

up-regulated expression of IFN-g and decreased TGF-b, IL-10 in the myocar-

dium. We also found BCG reversed AAC-induced increase in the number of

myocardium-infiltrate in M2-macrophages. Moreover, inhibition of TLR4 sig-

nificantly attenuated the protective effects of BCG, while mannan did not. Our

results suggest that BCG can prevent cardiac remodeling induced by pressure

overload by modulating immune microenvironment via activation of TLR4.

DAMAGE EFFECTS OF SLEEP DEPRIVATION ONMYOCARDIUM AND ITS ANTIOXYGEN INDEX IN RATSHui Juan Chai 1, Shu Mei Xu* 1, Hai Xian Yang 2

1 Department of Physiology, Tianjin Medical University, Tianjin,China2 Center of Electron Microscopy, Tianjin Medical University, Tianjin,ChinaSleep deprivation is a very common phenomenon in our society now and can

cause damaging effects to our body. Using the ‘‘flower pot technique’’ sleep

deprivation model, we demonstrated that sleep deprivation could cause damag-

ing effects on myocardium. After sleep deprivation, heart rate increased and S-

T segments of ECG rose or mixed with T waves showing ischemia of myocar-

dium, P-R interval and Q-T interval prolonged. In addition, cadiocytes lysis or

necrosis, subcellular organelles were impaired: the chromosome dissolved

gently, the endoplasmic reticulum expanded accompanied with evidence of

Ca2+ over loaded. Following morphological changes were observed: structure

of the mitochondria blurred; the intercalated disk dissolved; thrombocytes ac-

cumulated in microvessels; protein deposited in endotheliocytes interstitium;

edema, bleeding and monocytes invasion in the lumen; and lipid peroxidation

reaction effects spread widely. In addition to morphological changes, myocar-

dium mitochondrial showed increased malondialdehyde level and increased

superoxide dismutase activity followed by a decreased trend as sleep depriva-

tion prolonged. These results suggest that sleep deprivation can induce damage

on myocardium and the stress especially oxygen stress caused by sleep depri-

vation may be the possible mechanism, indicating potential use of antioxidants

to treat sleep deprivation.

DISTRIBUTION OF e 159 C/T GENE POLYMORPHISM INPROMOTER REGION OF CD14 AND ITS SIGNIFICANCEFOR PLASMA LIPIDS LEVELSFang ChenDepartment of Clinical Laboratory Science, Second XiangyaHospital, Central South University, Changsha, ChinaTo investigate the distribution of e159 C/T gene polymorphisms in the pro-

moter region of CD14 and its relation to plasma lipid levels in normal Chinese

Han population in Hunan, genotypes of CD14 were typed in 118 normal men

by PCR - RFLP and their plasma lipid levels were assessed. The - 159C/T

gene polymorphism was present in normal Han population of Hunan, and

the frequencies of CC, CT and TT were 19.5 %, 51.7 %, and 28.8 %,

respectively. The TT genotype carriers had lower plasma low - density lipopro-

tein cholesterol (LDL - C) level than that of the CT and TT genotype carriers

(P< 0.001, P< 0.05). No association was found between the genotypes and

plasma levels of total cholesterol, triglyceride, high -density lipoprotien, apo-

lipoprotein A1, apolipoprotein B100 and body mass index. TT genotype sig-

nificantly influenced the plasma levels of LDL - C in the normal Chinese

Han population.

RECONSTITUTION OF CORONARY VASCULATURE INISCHEMIC HEARTSHao Chen 1,2, Lei Cheng 2, Jie Ting Zhang 1, Ming Li 1,2, HsiaoChang Chan 1,2

1 Epithelial Cell Biology Research Center, Department of Physiology,Faculty of Medicine, The Chinese University of Hong Kong, Shatin,Hong Kong2 Joint Laboratory For Regenerative Medicine, The ChineseUniversity of Hong Kong, Shatin, Hong KongIn the remodeling process of post myocardial infarction, limited neoangiogen-

esis to the infarct-bed capillary is insufficient to support the greater demands

of the hypertrophied but viable myocardium, which results in further ischemic

injury to the viable cardiomyocytes at risk. Here we reported the rapid angio-

genic effect induced by the extracts from Dagencao (angio-T) to form func-

tional vasculature and the promoted survival potential of the viable

cardiomyocytes at risk after myocardial infarction. Our results demonstrated

dual effects of angio-T on up-regulating expression of angiogenic factors

and protection of the cardiomyocytes against further ischemic injury. Echocar-

diograph studies demonstrated significant functional improvement of the in-

fracted hearts by 2 days after infarction and angio-T treatment. These

therapeutic properties of angio-T to induce early reconstitution of a blood sup-

ply network, and improve heart function post infarction appear entirely novel

and may provide a new dimension for therapeutic angiogenesis medicine for

the treatment of ischemic heart diseases.

COATING PREPARATION AND DRUG DELAYED RELEASEIN VITRO AND IN VIVO OF DEXAMETHASONE-ELUTINGINTRAVASCULAR STENTSYong Peng Chen 1,2, Gui Xue Wang* 2, Xian Chun Jin 1, LaiLong Luo 2, Gang Zhang 1, Laichun Lu 1

1 Xinqiao Hospital, Third Military Medical University, Chongqing,China2 Bioengineering College and ‘‘111 Project’’ Laboratory ofBiomechanics and Tissue Repair, Chongqing University, Chongqing,ChinaTo observe the effect of drug delayed release in vitro and in vivo in dexameth-

asone-eluting intravascular stent, we prepared stents by dip coating, and then

high performance liquid chromatography (HPLC) was used to detect the drug

loading. The weight of bare stents and drug eluting stents were determined.

NETZSCH thermal analyser was used to analyze dexamethasone. The drug

slow-release rate in vitro was detected with parallel plate flow chamber perfu-

sion system, and then the dexamethasone-eluting intravascular stents were im-

planted into rabbit abdominal aorta to detect drug slow-release character in

vivo. The blood flow velocity of rabbit abdominal aorta was detected by ultra-

sonic inspection. The results showed the drug loading of stents prepared by dip

coating for four days was 93.15� 7.83mg. The weight of drug load was about

13.70� 0.84 % in the coating. Differential scanning calorimetry (DSC)

indicated that the melting point was 259.3�C, which was coincided with

254e264�C in the pharmacopoeia of the People’s Republic of China. The

thermogravimetry (TG) curve indicated the rate of loss in weight of dexameth-

asone was 76.67% from 250�C to 400�C. DSC and TG curve hinted the course

of evaporation and sublimation of dexamethasone on the temperature. Drugs

were released slowly and the release rate reached 84 % at 15d. The maximum

blood flow velocity of abdominal aorta was 27.03� 3.25 cm/s, while the

minimum velocity was 12.13� 2.20 cm/s. The drug concentration detected

after implantation 2 hours was 4.59mg/g in vessel wall and 1.91 mg/g in liver,

respectively. The drug concentration detected after implantation 10 days was