Recombinant Technology for ND and MD Vaccine strains

Early Protection…. Always Producing More

Fowl

Jungle Fowl

Domestication

Backyard

Fowl

Commercial Farm

Modern Farm

21 days 50 days

19802020

Cycle of 71 days… 30% hatchery… 70% farms

Cycle of 56 days… 38% hatchery …62% farms

35 days

Cycle of 71 days… 30% hatchery… 70% farms

Genetics : Broiler

Genetics : Layer

Chick 17 week 72 week 100 week.

330 488.5

Body weight (Broiler )

4.5 TIMES.50 TIMES.

Chick 1 Week 35 Days

40 GM 180 GM 2 KG

• Housing

• Feed

• Climate control

• Disease control

Hatchery

High quality Hatching eggHealthy parentsVaccination program for MDA

• Housing

• Feed

• Climate control

• Disease control

Biosecurity

Monitoring

Vaccination

KEEP BROILER PRODUCTION EFFICIENT

Optimize the processes

Ease the processes

Safeguard performance

Do as much as possible in the hatchery

hatchery vaccination- (s/c, in ovo)

•Equipment to standardize, less labor• Minimum and early reaction• No disturbance during growing period

MOVE VACCINATION AS

MUCH AS POSSIBLE INTO

THE HATCHERY

• Fewer hatcheries than farms ( Hatchery Vs Farms )

• Easier to control standardized procedures (SOP )

-Preparation and administration

• (semi) automatic equipment

-standardize, uniform application, reduce human factor

• Create uniform conditions

Easier to control temperature, relative humidity and light

• Introduction of new Hatchery vaccines

VaccinationEarly protection built from the hatchery

Vaccination

Vaccination Options

Conventional Method

Hatchery Vaccination

In ovo Vaccination

Farm level

Hatchery level

Hatchery level

Vaccination

Vaccination options

In ovo D18

Subcutaneous injection

At hatch

Spray/eye drop

Introduction of new Hatchery vaccines

Innovating with Science

Leading with Prevention

Advancing Well-being

Recombinant vaccines

• Recombinant vaccines are based on new technologies of molecular engineering

• It is all based on the technology of the recombination of the DNA (genetic engineering)

Recombinant vaccineswe have single construct and dual construct vaccine on the market

• Consists of a vector (virus or bacteria) expressing foreign antigens

• Genes that codify for 1 or more antigens (proteins) are introduced in the vector’s genome

• The immune response (cellular & humoral) is against the vector, including the inserted antigen(s), without the need of using the causative agent

2 in 1Marek + ND protection

Innovax-ND

2 in 1!

Nobilis ND CL30

HVT

HVT

ILTpart of ND virus

F-gene of ND Cl30

HVT

rHVT+ND/F

molecular engineeringConstruction of recombinant

Herpes virus

NDV disease of poultry

ND SB1 first generation“single construct”

rHVT+ND/F

Recombinant Innovax NDcombine 2 in 1

HVT

HVT

ILT

ND Cl30

Existing vaccines

Marek protection

Newcastle disease

protection

NDV - disease of poultry

Herpes virus

3/2/2020

How does it work

3/2/2020

Expression of F gene in NDVresponsible for F-protein on virus surface

F Gene

F Gene

ND VirusF Protein

F Protein

AttachmentHost cell

NDV Infection

3/2/2020

Mode of Action F Protein NDV:

Initiates the infection of host cell

Chicken cell

F protein fuses with receptor cell membrane

ND Virus F-protein

NDV Infection

3/2/2020

Insertion of the F gene of NDV in HVT genome

rHVT/F “Recombinant”

INNOVAX-ND single constuct

F gene + promoter & terminator

inserted in HVT viral genome

HVT Virus

ND virus

Construction of recombinant

3/2/2020

Mode of Action INNOVAX-ND Vaccine(HVT/F)

F protein

Foreign protein

Production

F antibody

HVT/F Recombinant replication

F geneHVT Glycoproteins

inducing immunity

against MD

3/2/2020

INNOVAX®-ND-SB1

The HVT part will still induce marek protection up

to the level of related to HVT

The F-protein will induce F-antibodies and

The F antibodies will neutralize the F protein of

ND virus “blocks” (prevents) the infection of the

chicken after a NDV field exposure.

3/2/2020

Action of INNOVAX-ND SB1 Vaccine

Chicken cell

F antibody

ND virus

3/2/2020

Action of INNOVAX-ND SB1

Chicken cell

F antibody

ND virus

Antibodies block attachment

3/2/2020Presentation Name

INNOVAX®-ND +SB1

Is a combination of HVT (ND )+SB1

To improve mareks protection up to the level of HVT+SB1

INNOVAX®-ND

Is a combination of HVT +ND

Advantages of a recombinant vaccine

• Absence of post vaccination reaction

• No interference with ND MDA or other vaccines

• No dissemination of vaccine virus

• Convenient and homogenous administration (in-ovo or day-old hatchery)

• Act as a marker vaccine (DIVA: Differentiate Infected from Vaccinated Animals )

• Ease vaccination program 2 in 1

Recombinant Vaccines: Characteristics

• Development of immunity depends of the replication of the vector

• Need of priming with conventional ND vaccines in high challenge areas

• Serological monitoring is different

Efficacy

Innovax ND-SB1

Challenge study ND

Protection from 2 weeks of age increasing tofull protection* by 4 weeks of age

MD challenge with vvMDV(RB1B)

5th Day – 94% Protection

0

20

40

60

80

100

11 15 21

% P

rote

cti

on

Age (days)

Development of protection after in ovo vaccination of SPF birds with Innovax-ND

Challenge studies with vvNDV strains from different genotypes and geographic areas

USA: Texas GB – Genotype II

Mexico: Chimalhuacan – Genotype V

Thailand: CU2 – Genotype VII

UK: Herts – Genotype IV

For our new dual construct (InnovaxND-IBD) in addition we also showed ND protection against GenotypeVII from Egypt and Belgium

As reference the Hitchner B1 and LaSota strains belong to genotype II

Challenge study ND

3 in 1Marek +ND+IBD protection

Innovax- ND-IBD

3 in 1!Marexine CA

Vector

HVT

ILT

Immunogenic parts of ND virus

F gene

HVTrHVT-F-VP2

Herpes virus

ILT

ND-IBD second generation“Double construct”

ILTF surface glycoprotein

Immunogenic parts of IBD virus

VP2 gene VP2 glycoprotein

NDCl30

Donor F gene

Faraghar

Donor VP2

Marek & Recombinant vaccine storageCell associated vaccines (CA)

LN2 (-196 C)

CA vaccines are frozen vaccines and should be stored under LN

Presentation title /35

•

Preparation of CA vaccines training as it is one of the major steps for the success

4 Key Points- Preparation

Needle Size – 18 G ( while mixing after thawing )

Thawing Time - 90-120 sec

Thawing Temp – 27 °C

Administration Time – 45 Min

work in hygienic way prevent contamination

37

In ovoDay-old Sub-cutaneous injection

RecombinantHatchery vaccination

Perfect fit in:

Innovating with Science

First dual recombinant vaccine ever in Animal Health

Revolutionary technology to control main viral diseases in poultry

Leading with Prevention

Hatchery vaccine solution to ND, IBD and ILT

Improved performance early vaccination

Advancing Well-being

No vaccine reaction

Reduced diseases incidence leading to less use of antibiotics

Recombinant Vaccines

Summary

Technology

Products

Automation

Thank you for saving my life

WVPA (India) ConferenceAdvances in Poultry Science for One Health

28th February 2020, Bangalore – Indiafor Membership and other info please Log

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