Recombinant DNA technology
Transcript of Recombinant DNA technology
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Today we will
• Understand what Diabetes is and how it affects the person
• Understand how insulin can be produced by recombinant DNA technology
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The problem - Diabetes
• What do you know?• Symptoms? • Cause? • Treatment?
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Insulin – what is it exactly?
• A hormone (chemical messenger) that allows glucose to pass from the blood into your cells.
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How it works
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The solution - how can we getb lots of insulin?
• Other animals?
Get something less cute to make it for us using the gene for insulin.
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Recombinant DNA technology
• Joining together DNA molecules from two different species. This is then inserted into a host organism which can produce new genetic combinations that are useful to us
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Bacteria – E. coli
Transformation
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And this is how…
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Steps (not in order)
The gene for insulin is isolated andremoved from human DNA leaving it with sticky ends
A plasmid from the bacteria E coli is removed and cut open using restriction enzymes
The insulin gene is inserted into the bacterial plasmid using DNA ligase
The recombinant plasmid is taken up by the bacteria by transformation
The bacteria reproduces, making copies of the gene each time, allowing lots of insulin to be produced
Key wordsIsolated gene Sticky ends Vector Plasmid Restriction enzyme DNA ligaseRecombinant plasmid Transformation
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What else?
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Recombinant D
NAPLASMID
Vector
Restriction
enzyme
Transformation
Insulin
Pancreas
Sticky ends
Gene
Host
DNA ligase
HORMONE