ReaPan B27 Reagent
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Transcript of ReaPan B27 Reagent
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8/14/2019 ReaPan B27 Reagent
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ReaMetrixAccelerating Innovation
Human Leukocyte Antigen B27 is a class I surface antigenencoded by the B locus in the major histocompatibility complex(MHC) that presents microbial antigens to T-Lymphocytes.The expression of HLA-B27 antigen is associated with theonset of a class of seronegative spondylo-arthropathies that
include ankylosing spondylitis, Reiters disease, psoriaticarthritis and inammatory bowel disease.
The ReaPan B27 reagent uses a two-color immunouorescencebased method for detecting HLA-B27 antigen on a owcytometry platform. The reagent is provided in a unitizeddry format containing predispensed amounts of materialadequate for a single test.. This reagent is intended for use onopen ow cytometer systems .
Qualitative test results are reported on the basis of a RMXvalue recorded on the sample acquisition template. RMX value
is a quantication of the relative dierence in uorescentstaining intensities of the sample and the reference beads. Apositive sign on the RMX value implies that the sample testedcan be typed as positive. A negative RMX value indicates thatthe expression of the HLA-B27 antigen is not sucient forthe sample to be typed as positive.
ReaPan B27A reagent or detection
o HLA-B27 Antigen
ConvenienceProvided as a dried-down reagent in a readyto use format; Easy storage and handling.
SpecicityAnti-B7 antibody minimizes cross-reactivityof Anti-B27 antibodies with the B7 antigen lower probability of false positives.
Internal Reerence BeadsNo external calibration required;internal reference beads provided inevery tube. Makes the test robust.
Simple WorkfowLyse-no wash protocol simplies and reducessample processing time signicantly.
Ease o ReportingSign of the RMX value indicates apositive or negative result.
SensitivityGating of T-lymphocytes (CD3+) to reduce
background; Use of two clones of Anti-HLA-B27 enhances sensitivity of the test.
Features
Ph: +91-80-28378693/5
E-mail: [email protected]
www.reametrix.com
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Take dry unitzed
dried-down reagent tube
Add 25l
blood sample
Vortex for 30 secs and
incubate for 30 min
Add 300l lysis solutonand incubate for 15 min
Acquisiton and
analysis by fow
cytometry+
30min
15min
Sample Workfo(ReaPan B
CD3-PE-Dy649 uorescence ver-sus side-scatter to capture CD3+(R1) and uorescence beads (R2)
HLA-B27-PE uorescence(FL2) (gated from R1 ofFigure 1) versus Counts
Fluorescent bead intensity(FL2) from R3 of Figure 1versus Counts
Sample analysis and gating using ReaPan B27 reagent
Product Information
ReaPan B27
Product Catalogue No.: 25238-00
Antibodies used:Labeled Anti-CD3 (Clone UCHT1)Labeled Anti-HLA-B27(Clones FD705 and ABC-m3)
Unlabeled Anti-B7 (Clone BB7.1)
50-B, II PhasePeenya Industrial AreaPeenya, Bangalore 560058, India
Ph: +91-80-28378693/5Fax: +91-80-41172451
E-mail: [email protected],
www.reametrix.com
ReaMetrixAccelerating Innovation
Intensity Controlreference beads
Selection ofT-Lymphocytes
T-Lymphocytesstained withAnti-HLA B27antibodies
Intensitycontrolreference beads
Figure 1 Figure 2
