Rb cs count
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Red cell count Red cell count
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Transcript of Rb cs count
Red cell countRed cell count
MethodsMethodsA. Manual diluting method.
Principle:Using a diluting fluid that destruct all blood cells
leaving only RBCs intact.
With the aid of a hemocytometer, counts the number of cells expressed in millions per micro liter of blood.
ReagentsReagents
Gower’s solution: Sod. Sulphate, Glacial acetic acid and D.W
Hayem’s solution: mercuric chloride, NaCl, sod. Sulphate and
D.W. Physiological saline (0.85%)
EquipmentsEquipments::
HemocytometerCover slipPipettes
ProceduresProcedures::1. Dilution:
Add 2 ml of hayem’s solution to clean and dry tube.
Add 10 µl of the sample (whole anti-coagulated blood) and mix.
Dilution factor: 1:200
2. Charging
Charge the hemocytometer between the cover slip and the counting chamber.
Leave the cells to settle down.The depth factor is 1\10
3. Counting:
Count the RBCs in 5 medium squares (4 at the 4 corners & 1 in the center) inside the central large square.
Count those cells in L-shape manner
16 Tertiary squares
Reading (counting)Reading (counting)????? Cell /square.
CalculationCalculation::Erythrocytes / µl of blood= cells counted per
square х 10 (depth) х 5 х200(dilution)
ORErythrocytes / µl of blood= cells counted per
square х 10 (depth)х 25х200 (dilution)
= …………………. Cells / µl or cells / mm3
B. Other automatic B. Other automatic methodsmethods : :
Electronic cell counterFlow cell cytometers : depends
on laser lights.
