Barcoding the Kelp Forests of

California’s Channel Islands National Park

Ralph Imondi, David Kushner, Daniel Distel, Timery Deboer, Robert Hanner,

and Linda Santschi

Rattus norvegicus Mus musculus Gallus domesticus

Danio rerio Drosophila melanogaster

Schistocerca nitens

Xenopus laevis

Caenorhabditis elegans Hirudo medicinalis

Energizing science through

interdisciplinary studies

concepts

tools processes

ecology

environmental

science & policy

molecular

biology

genetics

biochemistry

earth science

computer

science

cell biology developmental

biology

Mending the disconnect between

scientific research and science education

scientific research community 2˚ educational community

exploration

& discovery

community

analysis

&

feedback

benefits

&

outcomes

TESTING

IDEAS

Emphasizing the scientific discovery process

utilizing barcode data

application

user

research

user

general

public

GenBank BOLD

EOL

Potential student entry points into the

DNA barcoding workflow

specimen collecting and

tissue sampling

Student engagement in the entire workflow

specimen collecting

tissue processing

creating specimen pages on BOLD

generating COI amplicons

analyzing, editing, and uploading sequence data

California’s northern Channel Islands

The northern Channel Islands are fringed by

extensive kelp forest habitat

Upwelling features near the islands

contribute to kelp forest productivity

* * *

*

Point

Conception

Ventura

Santa Barbara

Los Angeles

California

Current

California

Countercurrent

cold subartic

water

warm equatorial

Pacific water

San Luis Obispo

Oregonian

Province

Californian

Province

The confluence of major current systems

gives rise to ecologically diverse species

assemblages

Santa Cruz Anacapa San Miguel Santa Rosa

variable transition zone

special

assemblage

temperate

biota

boreal

biota

Pycnopodia helianthoides Hypsypops rubicundus

The CINP monitors up to 37 sites around the

northern Channel Islands

San Miguel

Santa Rosa Santa Cruz

Anacapa

the sites reflect the broad range of conditions and

biological assemblages found in the park

O. pictus C. gigantea P. helianthoides C. punctipinnis

B. elegans H. rufescens M. californica S. californicus

U. lofotensis H. rubicundus M. undosa O. californica

The park collects abundance data for select

species of algae, invertebrates, and fishes

C. californica C. spadicea P. miniata C. nicholsii

P. stearnsii K. kelletii P. californicus E. jacksoni

L. chilensis M. crenulata D. imbricata L. dalli

Sebastes barcoding project

S. mystinus S. chrysomelas S. auriculatus S. rosaceus

S. dalli S. carnatus S. semicinctus S. serriceps

S. umbrosus S. atrovirens S. miniatus S. serranoides

number of species

number

of species

1 - 5

6 - 10

11 - 15

16 - 20

21 - 25

26 - 30

31 - 35

36 - 40

41 - 45

46 - 50

51 - 55

56 - 60

1960 1965 1970 1975 1980 1985 1990 1995 2000 2005 2010

1.0

0.8

0.6

0.4

0.2

0

rela

tive d

eple

tion

Rockfishes are a heavily exploited by

commercial and recreational fisheries

Pacific Ocean Perch

Widow

Yelloweye

Bocaccio

Darkblotched

Cowcod

Canary

The inability to identify larval rockfish

hinders efforts to characterize their

distribution, abundance and life history traits

Forms of student engagement

Direct (residential program for students)

Indirect (professional development program for teachers)

CMB

training

instructional materials

standardized lab protocols

transportable equipment

CMB

high

school

Collaboration framework (scientific strand)

specimen

collection & tissue

processing sample curation

& data accession

Ocean

Genome

Legacy taxonomic

verification

& vouchering

SBMNH

CCDB distribution

students

CINP resource management & enforcement personnel

UCSB Marine Operations Facility

volunteers

Economic workforce development linkages

Recruitment Equipment support

Residential program for students

Professional development program

CMB

high

schools

SoCal Biotech Center

LA/OC Biotech Center

North Valley Biotech Center

Bay Area Biotech Center

Recruitment

Equipment support

Economic workforce development linkages

Arcadia

Bakersfield

Calabasas

Camarillo

Carpinteria

Chatsworth

Compton

Dana Point

Dos Pueblos

Dublin

Fontana

Granada Hills

Hollywood

Huntington Beach

La Jolla

Lakewood

Long Beach

Los Angeles

Los Altos

Los Gatos

Los Osos

Mission Viejo

Moorpark

North Hollywood

Norwalk

Oak Park

Geographic Distribution of Participants

300 km

Project status

NORTHERN CHANNEL ISLANDS SEBASTES BARCODE PROJECT [CMBS]

Project status

Channel Islands Kelp Forest and MPA Fish and Invertebrates [CNOI]

Geographic Distribution of Participants

300 km

Mohave

Desert

Expanding student access to specimens

Sonoran

Desert

Klamath

Sierra

Nevada San

Francisco

Bay Area

Mediterranean

Coast

Alignment of student-led barcoding activities

with NPS I&M activities and youth programs

Technology Resource Development

Technology Resource Development

www.DNAbarcodingAssistant.org

Technology Resource Development

CMB Guelph

BOLD data portal

meeting the

barcode data

standard

user user

BOLD

Technology Resource Development

high

schools

Guelph

BOLD

data portal

meeting the

barcode data

standard

professional

user

BOLD

professional

user

non-professional

user

pre-BOLD

Biotechnology ExplorerTM

An Internet platform to broaden student

engagement in DNA barcoding and iBOL

Robert Hanner

Sujeevan Ratnasingham

Acknowledgements

David Kushner

Sonia Ibarra

Eric Mooney

Joshua Sprague

Milton Love

Samuel Hammond

Christoph Pierre

Dan Distel

Abigail Fusaro

Timery Deboer

Eric Hochberg

Paul Valentich-Scott

Jeffery O’Neal

Wendi Johnston