Racing Bacterial Cells in Microfluidic Gradients
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![Page 1: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/1.jpg)
Racing Bacterial Cells in Microfluidic Gradients
in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology
![Page 2: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/2.jpg)
Racing Bacterial Cells in Microfluidic Gradients
in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology
Why:
Length variation is observed in isogenic bacteria population
![Page 3: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/3.jpg)
Racing Bacterial Cells in Microfluidic Gradients
in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology
Why:
Length variation is observed in isogenic bacteria population
Does length variation have any functional role? → e.g. enhanced/diminshed motility?
![Page 4: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/4.jpg)
Racing Bacterial Cells in Microfluidic Gradients
in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology
Why:
Length variation is observed in isogenic bacteria population
Does length variation have any functional role? → e.g. enhanced/diminshed motility?
Aim: Physical model of how cell size and number of flagella relate to swimming speeds and efficiency in chemotaxis
![Page 5: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/5.jpg)
How:
Build microfluidics chamber using PDMS based soft-lithography
![Page 6: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/6.jpg)
How:
Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar)
→ Quantitative measurement of gradient by adding dye in same conc. → Simulating gradient with physics modeling program
chemoattractant bacteria
![Page 7: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/7.jpg)
How:
Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar)
→ Quantitative measurement of gradient by adding dye in same conc. → Simulating gradient with physics modeling program
Recording bacterias with DIC timelapse microscopy Identify single cells and measure their motion tracks (Matlab) as well as size
chemoattractant bacteria
![Page 8: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/8.jpg)
How:
Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar) → Quantitative measurement of gradient by adding dye in same conc.
→ Simulating gradient with physics modeling program Recording bacterias with DIC timelapse microscopy Identify single cells and measure their motion tracks (Matlab) as well as size
![Page 9: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/9.jpg)
Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar) → Quantitative measurement of gradient by adding dye in same conc.
→ Simulating gradient with physics modeling program Recording bacterias with DIC timelapse microscopy Identify single cells and measure their motion tracks (Matlab) as well as size
~1.5 µm thickness~ 3 µm spacing
![Page 10: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/10.jpg)
![Page 11: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/11.jpg)
Positions of tracked beads
![Page 12: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.fdocuments.in/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/12.jpg)