Quiz #5 (9%) Biol710 11/7/12 name___________

iz #5 (9%) Biol710 11/7/12 name______ t the three ingredients for a functional plasmid used in cloning: _____ ___ t is the value of a multiple cloning site? (not needed for plasmid functiona ______________________________________ 1% w an expression plasmid (all or none) whereby you can use it for testing enh #1 ATG TTAATTAA GAATTC, ATGA TTAATTAA GAATTC, ATGAA TTAATTAA GAATTC #2 ATG AGATCT GAATTC, ATGA AGATCT GAATTC, ATGAA AGATCT GAATTC #3 ATG TGATCA GAATTC, ATGA TGATCA GAATTC, ATGAA TGATCA GAATTC nning to make a plasmid library to over express unknown proteins using eye c dT primer for reverse transcription will have an EcoR1 site (GAATTC) on it, g between doing a Pac I (TTAATTAA) , a Bgl II (AGATCT) or a Bcl I (TGATCA) pen up the coding sequences for fusion into one of the three sets of vectors Q1 Q2 ch set of vectors would you choose? (1%)_____ two reasons why you wouldn’t choose the other two sets. (2%)__________ ___

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Quiz #5 (9%) Biol710 11/7/12 name___________. Q1. a) List the three ingredients for a functional plasmid used in cloning: _____ _____ _____ 3%. b) What is the value of a multiple cloning site? ( not needed for plasmid functionality! ) - PowerPoint PPT Presentation

Transcript of Quiz #5 (9%) Biol710 11/7/12 name___________

Quiz #5 (9%) Biol710 11/7/12 name___________

a) List the three ingredients for a functional plasmid used in cloning: _____ _____ _____ 3%

b) What is the value of a multiple cloning site? (not needed for plasmid functionality!)_____________________________________________ 1%

c) Draw an expression plasmid (all or none) whereby you can use it for testing enhancers: 2%

#1 ATG TTAATTAA GAATTC, ATGA TTAATTAA GAATTC, ATGAA TTAATTAA GAATTC

#2 ATG AGATCT GAATTC, ATGA AGATCT GAATTC, ATGAA AGATCT GAATTC

#3 ATG TGATCA GAATTC, ATGA TGATCA GAATTC, ATGAA TGATCA GAATTC

You are planning to make a plasmid library to over express unknown proteins using eye cDNA. Your oligo dT primer for reverse transcription will have an EcoR1 site (GAATTC) on it, but now youare deciding between doing a Pac I (TTAATTAA) , a Bgl II (AGATCT) or a Bcl I (TGATCA)digest to open up the coding sequences for fusion into one of the three sets of vectors below.

Which set of vectors would you choose? (1%)_____

Give me two reasons why you wouldn’t choose the other two sets. (2%)__________ ___________