Quantitative analysis of cytoskeletal network structures fileCytoskeleton Keratin filaments. Role of...
Transcript of Quantitative analysis of cytoskeletal network structures fileCytoskeleton Keratin filaments. Role of...
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Quantitative analysis of
cytoskeletal network structures
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5 µm
MTMF
IF (keratin)
microfilaments(MF)
persistence tensile length [µm] modulus [a.u.]
MF 18 300MT >100 150IF 1 1
intermediatefilaments (IF)
microtubules(MT)
Cytoskeleton
Keratin filaments
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Role of cytoskeletal filaments
1. cell and tissue architecture
2. cell migration
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Motility of leukaemia cells - vimentin
ATRAPMA
NB4 - Vimentin
neutrophil
Retinoic Acid Syndrome
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Tensegrity (R.B. Fuller, D. Ingber)
microtubulesmicrotubules
intermediate filaments
F-actin
substrate anchor
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Keratin networks in microgravity
t2µg
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control sphingosylphosphorylcholine (SPC)
Regulation of keratin networks
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min
F-actin
K8/18
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Keratin network morphology
perinuclear
periphery
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50 ng/mL TGFα
min
ERK
2 ac
tivat
ion
Seufferlein et al. 1999
control
TGFα (30 min)
Keratin network reorganisation in response to TGFα
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control
TGFα, 30 min
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control TGFα control TGFα
Structural features of keratin networks
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Fitting of random tessellation models
Poisson-Line (PLT) Poisson-Voronoi (PVT) Poisson-Delaunay (PDT)
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Iterated tesselations
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Bernoulli thinning
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Model selected as best fitcontrol TGFα
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control PVT / PLT
TGFα PDT / PLT
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Actin networks in lamellipodia
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Elasticity ~ mesh size4.4
PVT PDT PLT
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F-actin
Migration
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Stephanie Eckel, Frank Fleischer, Hendrik Schmidt, Volker SchmidtDept. of Stochastics, University Ulm
Paul WaltherDept. of Electron Microscopy, University Ulm
Thomas SeufferleinDept. of Internal Medicine I, University Ulm
Bishr OmaryDept. of Medicine, Stanford University
Tatjana SvitkinaDept. of Biology, University of Pennsylvania
Joachim SpatzDept. of Physical Chemistry, University Heidelberg
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