Quality Of Lamellar Dissections Prepared With The 500 Khz Femtosecond Laser System
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Quality Of Lamellar Quality Of Lamellar Dissections Prepared Dissections Prepared With The 500 Khz With The 500 Khz Femtosecond Laser Femtosecond Laser System System Volkan Hurmeric, MD, Sonia H. Yoo, MD, Mohamed Abou Shousha, MD, William W. Culbertson, MD, Noel Ziebarth, PhD, Jean-Marie Parel, PhD. Bascom Palmer Eye Institude Bascom Palmer Eye Institude Miami-FLorida Miami-FLorida Financial Disclosure: Financial Disclosure: Sonia H. Yoo and William W. Culbertson are on the speakers Sonia H. Yoo and William W. Culbertson are on the speakers bureau for Carl Zeiss Meditec bureau for Carl Zeiss Meditec
description
Quality Of Lamellar Dissections Prepared With The 500 Khz Femtosecond Laser System. Volkan Hurmeric, MD, Sonia H. Yoo, MD, Mohamed Abou Shousha, MD, William W. Culbertson, MD, Noel Ziebarth, PhD, Jean-Marie Parel, PhD. Bascom Palmer Eye Institude Miami-FLorida. Financial Disclosure : - PowerPoint PPT Presentation
Transcript of Quality Of Lamellar Dissections Prepared With The 500 Khz Femtosecond Laser System
Quality Of Lamellar Dissections Quality Of Lamellar Dissections Prepared With The 500 Khz Prepared With The 500 Khz Femtosecond Laser SystemFemtosecond Laser System
Volkan Hurmeric, MD, Sonia H. Yoo, MD,Mohamed Abou Shousha, MD, William W. Culbertson, MD,
Noel Ziebarth, PhD, Jean-Marie Parel, PhD. Bascom Palmer Eye InstitudeBascom Palmer Eye Institude
Miami-FLoridaMiami-FLorida
Financial Disclosure:Financial Disclosure:Sonia H. Yoo and William W. Culbertson are on the speakersSonia H. Yoo and William W. Culbertson are on the speakers
bureau for Carl Zeiss Meditecbureau for Carl Zeiss Meditec
Quality of Lamellar Dissections Prepared with the 500 Khz Femtosecond Laser SystemPurpose:
– To study the quality of lamellar dissection prepared with the 500 kHz femtosecond laser (FL)
Materials and Methods: – 6 human globes were cut with the VisuMax 500 kHz FL. – 200, 300, and 400 micron deep cuts were made each at low
(180 nJ) and high (340 nJ) energy settings [1.6 µ spot and line sep].
– Environmental scanning electron microscopy (eSEM) and atomic force microscopy (AFM) were performed on the tissue to assess the quality of the cuts.
Results
eSEMeSEMCut depth : 200 µm
Energy : 180 nJCut depth : 200 µm
Energy : 340 nJ
The 200 micron cut with high energy settings resulted in smooth dissections.
Results
AFMAFMCut depth : 200 µm
Energy : 180 nJ
Cut depth : 200 µm Energy : 340 nJ
Results
eSEMeSEMCut depth : 300 µm
Energy : 180 nJCut depth : 300 µm
Energy : 340 nJ
The 300 micron cut with low energy settings resulted in the smoothest dissection.
Results
AFMAFMCut depth : 300 µm
Energy : 180 nJ
Cut depth : 300 µm Energy : 340 nJ
Results
eSEMeSEMCut depth : 400 µm
Energy : 180 nJCut depth : 400 µm
Energy : 340 nJ
The 400 micron cut with low energy settings resulted in the roughest dissection.
Results
AFMAFMCut depth : 400 µm
Energy : 180 nJ
Cut depth : 400 µm Energy : 340 nJ
Roughness from AFM scans
0.0 1.0x10-5 2.0x10-5 3.0x10-5 4.0x10-5 5.0x10-5-2.0x10-6
-1.0x10-6
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2.0x10-6
200m 300m 400m
Hei
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High Energy
0.0 1.0x10-5 2.0x10-5 3.0x10-5 4.0x10-5 5.0x10-5
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Low Energy
200 and 300 micron lamellar cuts with the 500 kHz VisuMax FL result in excellent quality dissections.
“High frequency” roughness is lowest for the 300µm scans (both low and high energy).
Depth of cut and laser energy settings seem to affect the quality of the dissection.
Conclusions
