Pyruvate Oxidation and Krebs Cycle - Weebly

Pyruvate Oxidation and Krebs Cycle

Saturday, October 14, 17

recall that glycolysis involved one splitting glucose producing 2 molecules of each: NADH, H2O, ATP and pyruvate


Stage 2: Pyruvate Oxidation

occurs in matrix of mitochondria

when O2 is present, the 2 pyruvate molecules made in glycolysis get transported through transport proteins in to enter the matrix


Overall equation for pyruvate oxidation:

2 pyruvate + 2NAD+ + 2 CoA → 2 AcetylCoA + 2NADH + 2H+ + 2CO2

* CoA stands for Coenzyme A


3 changes happen in one step:Cytoplasm

Matrix of Mitochondria

Pyruvate




Pyruvate

CO2 1) Remove CO2




Pyruvate

CO2NAD+

NADH

1) Remove CO2

2) 2C complex gets oxidized to become acetate




Pyruvate

Acteyl CoA

CO2NAD+

NADH

Coenzyme A

1) Remove CO2

2) 2C complex gets oxidized to become acetate

3) Coenzyme A attaches to acetate via S, forms acetylCoA


Final Products of Pyruvate Oxidation:

2 NADH (saved for ETC)

2 AcetylCoA

can either be stored as fat or it well enter Krebs to create ATP


Occurs in the matrix of the mitochondria

Stage 3: Kreb’s Cycle


Overall equation for Krebs Cycle:

oxaloacetate + acetylCoA + ADP + P + 3 NAD+ + FAD → CoenzymeA + ATP + 3NADH + FADH2 + 3H+ + oxaloacetate + CO2


Citrate (6C)


Citrate (6C)

aconitate (6C)

H2O


Citrate (6C)

aconitate (6C)

isocitrate (6C)

H2O

H2O


Citrate (6C)

aconitate (6C)

isocitrate (6C)

a-ketoglutarate (5C)

H2O

H2O

CO2 NADNADH


Citrate (6C)

aconitate (6C)

isocitrate (6C)


succinyl CoA (4C)

H2O

H2O

CO2 NADNADH

NADNADH

CO2CoA


Citrate (6C)

aconitate (6C)

isocitrate (6C)


succinyl CoA (4C)

succinate (4C)

H2O

H2O

CO2 NADNADH

NADNADH

CO2CoA CoA


Citrate (6C)

aconitate (6C)

isocitrate (6C)


succinyl CoA (4C)

succinate (4C)

H2O

H2O

CO2 NADNADH

NADNADH

CO2CoA CoA

GDP

GTP


Citrate (6C)

aconitate (6C)

isocitrate (6C)


succinyl CoA (4C)

succinate (4C)

H2O

H2O

CO2 NADNADH

NADNADH

CO2CoA CoA

GDP

GTPADP

ATPSaturday, October 14, 17

Citrate (6C)

aconitate (6C)

isocitrate (6C)


succinyl CoA (4C)

succinate (4C)

fumarate (4C)

H2O

H2O

CO2 NADNADH

NADNADH

CO2CoA CoA

GDP

GTPADP

ATP

FADFADH2


Citrate (6C)

aconitate (6C)

isocitrate (6C)


succinyl CoA (4C)

succinate (4C)

fumarate (4C)

malate (4C)

H2O

H2O

CO2 NADNADH

NADNADH

CO2CoA CoA

GDP

GTPADP

ATP

FADFADH2

H2O


Citrate (6C)

aconitate (6C)

isocitrate (6C)


succinyl CoA (4C)

succinate (4C)

fumarate (4C)

malate (4C)

oxaloacetate (4C)

H2O

H2O

CO2 NADNADH

NADNADH

CO2CoA CoA

GDP

GTPADP

ATP

FADFADH2

NAD

NADH

H2O


Citrate (6C)

aconitate (6C)

isocitrate (6C)


succinyl CoA (4C)

succinate (4C)

fumarate (4C)

malate (4C)

oxaloacetate (4C)

Acetyl CoA (2C) CoA *Recycled to pyruvate oxidation

H2O

H2O

CO2 NADNADH

NADNADH

CO2CoA CoA

GDP

GTPADP

ATP

FADFADH2

NAD

NADH

H2O


CooksApproved

InKitchens

ServeSavory

FoodMoreOften

CitrateAconitateIsocitrate

KetoglutarateSuccinyl CoA

SuccinateFumarateMalate

Oxaloacetate


Net energy from Krebs

2 ATP, 6 NADH, 2 FADH2


Net energy at the end of Krebs

4 ATP (2 glycolysis, 2 Krebs)

10 NADH (2 glycolysis, 2 PO, 6 Krebs)

2 FADH2


Pyruvate Oxidation and Krebs Cycle - Weebly

Documents

Transcript of Pyruvate Oxidation and Krebs Cycle - Weebly