Purple Non Sulfur Bacteria
description
Transcript of Purple Non Sulfur Bacteria
![Page 1: Purple Non Sulfur Bacteria](https://reader035.fdocuments.in/reader035/viewer/2022062218/568166f0550346895ddb4d44/html5/thumbnails/1.jpg)
Purple Non Sulfur Bacteria
Hopkins Microbiology CourseAnn Lesnefsky 7-15-2011
![Page 2: Purple Non Sulfur Bacteria](https://reader035.fdocuments.in/reader035/viewer/2022062218/568166f0550346895ddb4d44/html5/thumbnails/2.jpg)
Metabolism of PNS Bacteria
• Organic acid electron donor• Photosynthesis for energy
generation– Bacterial chlorophyll b– Absorbs light in the infrared
range• Currently PNS bacteria are
being studied for H2 production
![Page 3: Purple Non Sulfur Bacteria](https://reader035.fdocuments.in/reader035/viewer/2022062218/568166f0550346895ddb4d44/html5/thumbnails/3.jpg)
Course Enrichment Method for PNS
• 6-18-2011– Plated samples from the slough on succinate and
ethanol plates, incubated anaerobically with light– Course’s media and Holly’s media
• 6-29-2011– Restreak single colonies for isolation
![Page 4: Purple Non Sulfur Bacteria](https://reader035.fdocuments.in/reader035/viewer/2022062218/568166f0550346895ddb4d44/html5/thumbnails/4.jpg)
TA Enrichment Method for PNS
• TA’s enriched for PNS by filtering– Filter both water above mat and
slurry of mat material– Place filter face down on plate– Flip and all allow PNS bacteria to
grow on filter
![Page 5: Purple Non Sulfur Bacteria](https://reader035.fdocuments.in/reader035/viewer/2022062218/568166f0550346895ddb4d44/html5/thumbnails/5.jpg)
Carbon Source
Enrichment Method
Succinate (# of colonies before restreak)
Ethanol (# of colonies before restreak)
No filter Course
43 6
Holly
0 0
Filter Course
Many
Some
Holly
Few None
![Page 6: Purple Non Sulfur Bacteria](https://reader035.fdocuments.in/reader035/viewer/2022062218/568166f0550346895ddb4d44/html5/thumbnails/6.jpg)
Microscopy of PNS• Colonies isolated from 5 different plates– 4 plates from strawberry– 1 from Kerby Park
• All looked like stubby rods
![Page 7: Purple Non Sulfur Bacteria](https://reader035.fdocuments.in/reader035/viewer/2022062218/568166f0550346895ddb4d44/html5/thumbnails/7.jpg)
Recommendations
• Next year use the filter method.
![Page 8: Purple Non Sulfur Bacteria](https://reader035.fdocuments.in/reader035/viewer/2022062218/568166f0550346895ddb4d44/html5/thumbnails/8.jpg)
Questions?