Purifier Logic Class II Biological Safety Cabinets Presentation

Purifier® Logic® Class II Biological Safety Cabinet

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Fume Hood or Biological Safety Cabinet?

Fume HoodsFume Hoods -- Enclosures that capture, contain and remove chemical fumesand vapors.

Biological Safety CabinetsBiological Safety Cabinets --Enclosures that capture, contain and remove biohazardous aerosols.

Purifier® Logic® Class II Biological Safety Cabinet

Optional 254 nm UV lamp

Bright, glare-free fluorescent lighting

Fully-closing, counterbalanced tempered safety glass sash

Large 21.7” sash opening height

Leak-tight Type 304 stainless steel liner

Type 304 stainless steel dished work surface with lift out knobs

Contain-Air Negative Pressure Channel

Innovative LCD information center display

Easy-to-clean touchpad controls

Reserve-Air Secondary Airflow Slots

Curved stainless steel inlet grille

Accessory Base Stand (sold separately)

Being Green is being responsible

Labconco is a member of the United States Green Building Council (USGBC).Three Labconco product lines contribute to LEED® points:

Purifier® Logic® Biosafety CabinetsFlaskScrubber® and SteamScrubber® Laboratory Glassware WashersProtector® XStream® Laboratory Fume Hoods

Purifier® Logic® Biosafety Cabinet

Purifier® Logic® Motor

New motor technology• Electronically Commutated Motor (ECM)• Over 3 million in use

Customer Advantages• Quieter • Cooler Operation • Energy savings of 60%• More reliable for correct airflow maintenance• Continuously outputs information to be

displayed and triggers alarms when out of parameters

Intelligence Built In


Why is it cooler and quieter?• The ECM is more energy

efficient ~ less energy is wasted as heat


How do we know it is running okay?• The motor is programmed to maintain constant

airflow as the filter loads, no sensors needed

How does that work?• Each BSC is tested at various airflows and

volumes• Software uses data to generate a motor-blower

curve programmed into each motor• The motor remembers the speeds and torques

required as filter pressures change

Intelligence Built In


How do we know the ECM is running correctly?• The ECM continuously outputs information to be displayed and

triggers an airflow alert when the motor speed changes suddenly

How do we know to change the filters?• Motor initial and maximum speeds are calculated and filter life

remaining is figured

The conventional motor used in biosafety cabinets is a Permanent Split Capacitor (PSC) motor. It is known as an induction motor.

Labconco Delta (pre-2008)NuAire LabGardESCO Labculture

ECM PSC motorPermanent magnet on the rotor Magnetic field must be inducedMore efficient Generates a lot of waste heatPerformance feedback No feedback


How the Logic ECM is betterHeat is the #1 killer of motors because it reduces the life of the bearing/bushing lubricant. Because the PSC runs hotter, it will fail faster than an ECM.The ECM uses hardened ball bearings on the rotor-most PSC motors use simple bushings. Cooler operation and bearings in the ECM means a much longer lifespan for the ECM…rated to 50,000 hours.


Logic ECM energy comparison

The ECM saves significant energy compared to other motor technologies used in biosafety cabinets.

Power Consumption (Watts)*

ECM PSC 3-Phase4' BSC** 290 582 4806' BSC** 490 1440 804

*At 120V** 10" sash height



LCD Information CenterLine-of-sight on inside wall

Filter status

Cabinet status

Error messages

Icons indicate features on/off

Interval or “stop watch” timer

Fluorescent/UV Hour meter with alarm

LCD Information CenterStatus area

• “OK”• “Sash is too high”• “Airflow Alert”• “System Error”

Data area• Displays the filter remaining,

inflow and downflow velocitiesIcon Area

• Displays icons of active features


IconsNormal blower operation

Night-Smart blower operation

Fluorescent light

UV light

Timer

Security Lock

Mute



Simple interior design• Easy to clean• Electrical duplex up and

out of work surface area• Accessories keep work

area uncluttered• Improved lighting

Dual fluorescent lamps instead of one


Simple interior design• No thumb screws • Lifting knobs on both ends• Work surface support to assist for

removal• Towel catch/Pre-filter retained

without fasteners• Smooth work surface

Towel Catch

Work Surface Support

Purifier Logic® Biosafety Cabinet

Sash Changes• Viewing height increased 1.75"• Angled sash with no sash handle

across the bottom edge for better viewing

• Formed handles attached to sash for easy gripping


Lower exterior height • Approx. 3/4" lower overall

Greater interior height• Approx. 2" taller in the front

Increased viewing height

Dimensions

Purifier® Logic® Accessories

Improved Filter Options• Accept ULPA filters• Optional pre-filters• Optional carbon post-filters on 4' - 6' models

Carbon Filtered ExhaustCarbon Filtered ExhaustStickStick--on Foam on Foam PrefilterPrefilter

Purifier® Logic® Biosafety Cabinets

Telescoping Base Stand

Adjusts in 1 inch increments from 27" to 34.5"NSF-listed and ADA-compliantDurable epoxy coatingAvailable with fixed leveling feet or toe-locking polyurethane castersSeismic models available

Accessories


Hydraulic Lift Base Stand

Built-in electric or manual hydraulic lift

Height is adjustable from 25.5" to 33.5"

Supports loads up to 1000 pounds

Available in 3', 4', 5' and 6' widths

NSF-listed and ADA-compliant

Left: Manual Hydraulic Lift Base Stand is shown with Caster Kit installed.

Accessories


Optional Wire Shelves (stainless steel snap-in)Optional Cord/Tubing management hooks

Accessories


Stainless Steel TurntableVacuum Aspiration Pump

Accessories

Airflow Sensor Kit (not shown)

• Kit allows for installation of airflow sensors in the field• Optional sensor can be factory installed• Recognizes sensor and displays inflow (after calibration)


Accessories

IV Bar supports intravenous solution bottles and bags

UV Light Kits include one 254 nm UV lampService Fixture


Provides convenient supply storage

Fits easily under the Purifier Logic Class II Safety Cabinets mounted on Base

Stand

2" casters provide mobility

Logic® Cart

Accessories


Accessories

Ergonomic Chair• 6-way articulating seat• Removable armrests

Adjustable Footrest• Elevate feet and permits angle

repositioning while in use


Accessories

Support Shelves• Provide work surface to support remote

equipment• Epoxy-coated steel shelf mounts to left-side

frame of base stand

Laptop Computer Arm• Securely supports laptop computer• Articulating arm raises, lowers and pivots to

a steady position

Purifier® Logic® Agency Approvals

NSF Standard 49 Listed• With carbon post-filter and pre-filter

installed• With accessory shelves, hooks, and IV bar

in place

ETL, ETL-C and CE markAccessory Base stand tip tested by NSF

Working in Biological Safety Cabinets

Planning

Thoroughly understand procedures and equipment required before beginning work

and arrange for minimal disruptions

Have disinfectant and spill cleanup materials prepared


Start Up

Turn off UV light, open sash to its proper height, and turn on cabinet lights and blower

Check grille for obstructions and let the cabinet operate for 5 minutes

Wash hands and arms thoroughly with disinfectant soap; wear a long sleeved lab coat and gloves

Use eye protection


Wipe-Down

Wipe down all interior surfaces of the work area with a solution of 70% ethanol or other suitable disinfectant


Loading

Load only the materials needed. Do not overload the cabinet or obstruct the grille. Keep large objects separated.

Lower the sash until it is in its proper position. Allow the unit to operate for 2 to 3 minutes to purge any airborne contaminants.


Work Techniques

Segregate clean and contaminated materials. Arrange materials to minimize movement of contaminated materials into clean areas. Keep all contaminated material in the rear of the work area.

Avoid excessive movement of arms or materials through the front opening during operation.

Keep materials at least 4" inside the sash opening and perform all contaminated operations as far to the rear of the work area as possible.


Work Techniques

Use proper aseptic technique.

Avoid techniques that disrupt airflow patterns in the cabinet, such as an open flame.

If there is a spill or splatter during use, all objects must be decontaminated before removal. Thoroughly

disinfect the interior surfaces of the cabinet while it is still in operation.


Final Purging

After completing work, allow the cabinet to operate for 2 to 3 minutes undisturbed to purge airborne contaminants from the work area.


Wipe-Down

Periodically lift the work surface and clean underneath it. Clean the towel catch.

Dispose of rubber gloves and have lab coat properly laundered. Wash arms and hands thoroughly with germicidal soap.

Wipe down all interior surfaces of the work area with a suitable disinfectant.


Shutdown

Turn off the fluorescent light and cabinet blower, close the sash and turn on the UV light if appropriate.

Cabinet Maintenance

User Performed Maintenance

Replace fluorescent and UV lamps annuallyReset circuit breakersAirflow smoke testEstablish a smoke split

• Mark work surface where smoke split occurs• May use smoke stick or dry ice to determine

where split occurs • User should work behind smoke split

Cabinet Maintenance

Certification

A validation of cabinet integrity and performance

Performed by a qualified independent technician

Performed when the cabinet is newly installed, annually thereafter, or if the

cabinet is moved to a new location

Cabinet Maintenance

Certification Tests

HEPA filter leak testInflow and downflow verificationAirflow smoke pattern testElectrical safetyVibration, sound and lighting intensity tests (optional comfort tests)

Cabinet Maintenance

What to Look For in a Certifier

Accredited by an independent organization (NSF, AFCA, CETA)

Has attended cabinet manufacturer certifier training course

Membership in professional organizations

Experience with the cabinet type and model

References

Written estimates before work is begun

Operating Procedures

Myths, Lies and Gobbledygook

During operation, the flame of a burner is very disruptive to the airflow patterns of the cabinet, and may actually increase the dispersion of aerosols throughout the work area.

If the flame of the burner is too large, the excessive heat may melt the adhesive holding the HEPA filter together or literally burn holes in the filter media. An unattended burner may blow out. In a Type A1 or A2 cabinet, the gas may reachexplosive concentrations.

"I've got to use a Bunsen burner in my Biosafety Cabinet..."

Use alternate methods, such as electric incinerators, or disposable inoculating loops. Don’t flame bottle mouths; the work area of the biosafety cabinet should be a sterile environment, if used properly.



The Fume Hood is designed to remove noxious or toxic fumes and aerosols away from the operator. It should be constructed of materials that are inert to a wide variety of chemical agents.

The Biosafety Cabinet's primary purpose is to protect the operator, environment, and often the product from biohazardous contaminants. The biosafety cabinet and its HEPA filters are constructed of materials that are inert to the chemicals used in connection with biological research, but may be damaged by some of the more corrosive chemicals commonly used in fume hoods.

"I can use a biological safety cabinet as if it were a fume hood..."

Do NOT use a biosafety cabinet like a fume hood!



Wrong. The biosafety cabinet will provide Personnel and Product protection only if used properly. Aseptic technique must be practiced at all times while working in a Biohazard Cabinet.

"If I work in a biohazard cabinet, I don't have to be as careful with my technique."



Yes. The UV light is only an adjunct, to minimize contamination of the work area when the cabinet is not in use. UV light has practically no penetrating power, and will not kill microbes protected by dust, dirt, or organic material.

The best method to prevent contamination in the cabinet is regular decontamination of the work area surfaces, before and after each work session.

"If I use the UV light, do I need to decontaminate the work area?"



You shouldn’t. Large objects placed in the work area will impede the airflow, reducing the efficiency of the cabinet. Electrical appliances like centrifuges, blenders, etc. often have cooling fans that can disrupt the cabinet’s airflow. It’s better to use sealed safety cups in the centrifuge that are loaded and unloaded in the safety cabinet.

"Can I put a centrifuge in the biosafety cabinet? "



Not Necessarily. If you’re talking about protection from volatile toxic chemicals, yes; if your talking about protection from biohazardous aerosols, no.

Assuming the units being considered are both NSF listed, they were both subjected to the same biological challenge testing. Any claims of superior containment should be documented.

"A Type B cabinet will give better protection than a Type A."



In some applications, such as working with cytotoxic drugs, it is recommended the cabinet operate continuously, to prevent the escape of toxic material.

In most applications, operating the cabinet continuously will only shorten the life of the HEPA filters.

"All biohazard cabinets should operate continuously.”



Assuming you have an air-tight room, with no ventilation system, and no activity in it, then a recirculating Type A cabinet mightclean the air to Class 5 levels. Unfortunately, as soon as the user entered the room, particle-laden air would re-contaminate the room.

All this will really do is shorten the life of the HEPA filters.

"If I leave the biosafety cabinet operating continuously, it will clean the air to Class 5 conditions.”



Not necessarily, HEPA filters should be replaced when the pressure differential across them becomes excessive. The Purifier Logic Filter Life gauge will alert the user when the HEPA filters should be replaced.

The two biggest factors in filter life are the level of contaminants in the room air, and how many hours the cabinet operates.

Arbitrarily replacing HEPA filters on a timetable, without regard to loading may be unnecessarily expensive and wasteful.

"I’ve heard that you should replace HEPA filters annually.”

Labconco Corporation 8811 Prospect Ave.

Kansas City, MO 64132

1-800-821-5525 816-333-8811

www.labconco.com

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Purifier Logic Class II Biological Safety Cabinets Presentation

Health & Medicine

Transcript of Purifier Logic Class II Biological Safety Cabinets Presentation