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Transcript of pure - LabXphotos.labwrench.com/equipmentManuals/14233-5498.pdfUsing this optional feature in...
> Reality requires a chromatography solution that can be tailored to new needs
Whether your operation is small or large, one of the things that you know for sure is that your research
goals are likely to change. You need a solution that can be adapted as your needs evolve.
ÄKTA pure grows with your research
> Science needs protein purification to be easy
Regardless of your field of research, purified protein is often a necessity to accomplish your goals.
You need a solution that helps you spend less time on purifying proteins and more time focusing on the results.
ÄKTA pure lets you focus on your research
> In a competitive world, my chromatography system should just do the job
Research has never been as competitive as it is today. Protein purification is often one step of many in a much
larger research process. You need a solution you can rely upon to produce your pure proteins in time for when
they are needed.
ÄKTA pure builds on more than 50 years of protein purification expertise
There’s an ÄKTA pure for everyone Discover yours
Pure convenience
I need to get onwith my research
Pure cooperation
We’re all working on different projects
In our lab, we have to share the same chromatography system. None of us are purifying lots of protein, but we all need to do it at times. We need a system that’s easy to share and maintain together.
I develop assays that need pure proteins. I am not really comfortable purifying proteins, so I need a system that’s easy to work with.
For local office contact information, visit:
www.gelifesciences.com/contact
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
www.gelifesciences.com/aktapure
GE, imagination at work and GE monogram
are trademarks of General Electric Company.
ÄKTA and UNICORN are trademarks of GE
Healthcare companies.
© 2012 General Electric Company
All rights reserved.
First published Aug. 2012
GE Healthcare UK Limited
Amersham Place
Little Chalfont
Buckinghamshire, HP7 9NA
UK
GE Healthcare Europe, GmbH
Munzinger Strasse 5
D-79111 Freiburg
Germany
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327
Piscataway, NJ 08855-1327
USA
GE Healthcare Japan Corporation
Sanken Bldg., 3-25-1
Hyakunincho Shinjuku-ku
Tokyo 169-0073
Japan
Reverse-flow elution increases sample concentration Bypass for automation of cleaning Detection of standard proteins at 280 nm
Four inlet positions enable automatic system and column cleaning while running overnight
Fraction collection in tubes
We explore new proteins and want to learn more about their function. The most interesting proteins are often really difficult to purify. But has breaking new ground ever been easy? I need an efficient system to help me explore.
We purify mostly antibodies for our discovery programs, but also some drug targets and endogenous proteins. We are always looking to deliver more and keep costs down. What we need is more automation.
Pure automation
We need more efficiency
Pure exploration
Getting my proteins as pure as I need them is a real challenge
Completely load multiple samples automatically
Connect up to five columns, with automatic switching among them, with integrated pressure sensors for column protection
Detect any biomolecule with triple wavelength UV detection
Select multiple buffers and wash solutions with 14 inletswith integrated air sensors for system protection
Control gradients and elution conditions with pH and conductivity monitors
Use tubes or plates for flexible, high capacity fraction collection
ÄKTA™ pureProtein purification your way
GE HealthcareLife Sciences
> Intuitive and easy to use
Whether you are an expert or an absolute beginner in protein purification, ÄKTA pure makes routine protein purification easy.
The flow path is forward facing and organi-zed so that it is easy to understand how buffers and samples flow.
Convenient valves enable basic automation such as running overnight and including automatic cleaning steps.
> Fast method set-up
ÄKTA pure works with any column, but if you use one of ours, the method to run it is already defined in UNICORNTM 6 software.
If you would like to customize your method, it is easily achieved using the drag-and-drop interface.
For direct and full control of your runs, the software provides a user-friendly, interactive process picture.
> Add functionality when you need it
What if your next project takes an unexpec-ted turn to, for example, require the use of more automation or the use of a special detection method?
Relax. With the flexibility provided by ÄKTA pure in terms of valves, detectors, pumps, and software you can add almost any functionality you need to overcome any purification challenge.
If you need to run at low temperature, ÄKTA pure is small enough to fit inside a standard cold cabinet.
> Go quickly from one purification task to the next
With ÄKTA pure, turnaround from one purification task to another can be done in only a few minutes.
With a bypass function for your column and with multiple buffer inlet positions, cleaning can be automated to make sure the system is ready-to-use immediately after a run.
Changing sample application method from, for example, direct loading to injection from a loop is now simply done by a few clicks of a mouse due to the convenient injection valve.
> UNICORN on every computer
Because UNICORN 6 licenses are for concur-rent users, you can to freely install and access the software on as many computers as you like.
Add licenses as your usage of the software increases. Further, results and methods are easily shared within the team via the UNICORN central database.
The optional UNICORN column logbook keeps track of column performance and important run data, providing traceability and increased operational security, especially if columns are shared.
> Convenient, prepacked columns
We provide a vast selection of columns prepacked with our modern chromatogra-phy media providing solutions for conve-nient purification of tagged proteins and antibodies, but also gel filtration, IEX and HIC techniques, to match your varying needs for speed, purity, and yield.
The so called “crude concept” column design simplifies your workflow by enabling purification directly from crude, homogeni-zed, unclarified cell lysate.
> Automate the task
With ÄKTA pure, automation possibilities are endless—multiple buffer inlet positions, safe loading of up to seven samples completely, the ability to automatically switch between up to five columns, and flexibility to allow automatic two-step purification.
Add to that a fraction collector that can collect in tubes as well as microplates and ÄKTA pure will fit any purification workflow.
> From one system to a whole facility
UNICORN is a software platform that is common to ÄKTA systems from research- to process scale. The software can run in a network such that methods and data can be shared between all users and systems.
Sharing provides full flexibility and enables optimal use of available systems.
With UNICORN software and ÄKTA pure systems running in a network, increasing capacity by adding more systems is easy.
> Run unattended with confidence
ÄKTA pure automation valves have been designed to maximize your productivity.
Failures are eliminated with inlets safeguar-ded by integrated air sensors.
Columns are protected from overpressure by integrated pressure sensors controlling the flow.
Should a system alarm go off or if you simply want to know when the run is completed, UNICORN 6 can automatically send an e-mail notification so that you can take immediate action.
> Screening for optimal conditions made easy
Screening for optimal chromatography conditions is an important first step to maximize protein purity.
ÄKTA pure column selection valve allows automatic screening on multiple columns.
The loop valve enables, for example, scree-ning using the same sample in multiple conditions, and multiple buffer inlets can be used to screen for optimal buffer conditions.
> Detection to verify and evaluate any method
Several monitors to suit your application can be fitted to ÄKTA pure.
Up to two UV monitors can be combined. The pH valve in combination with the conducti-vity monitor gives you full verification of elution conditions.
Further, easy connection of external monitors for, for example, fluorescence, refractive index, and light scattering detec-tion applications greatly expands your possibilities.
> Integrated Design of Experiments (DoE)
DoE provides a structured approach to method optimization.
Using this optional feature in UNICORN 6 software, it is not only possible to plan an experimental series to maximize the amount of information obtained from a minimum number of experiments; the system can also run it in an automated fashion.
Once the experiment has been run, the results are used to create statistical models to determine the optimal chromatography conditions to be used going forward.
> Reality requires a chromatography solution that can be tailored to new needs
Whether your operation is small or large, one of the things that you know for sure is that your research
goals are likely to change. You need a solution that can be adapted as your needs evolve.
ÄKTA pure grows with your research
> Science needs protein purification to be easy
Regardless of your field of research, purified protein is often a necessity to accomplish your goals.
You need a solution that helps you spend less time on purifying proteins and more time focusing on the results.
ÄKTA pure lets you focus on your research
> In a competitive world, my chromatography system should just do the job
Research has never been as competitive as it is today. Protein purification is often one step of many in a much
larger research process. You need a solution you can rely upon to produce your pure proteins in time for when
they are needed.
ÄKTA pure builds on more than 50 years of protein purification expertise
There’s an ÄKTA pure for everyone Discover yours
Detection of standard proteins at 280 nm
> Reality requires a chromatography solution that can be tailored to new needs
Whether your operation is small or large, one of the things that you know for sure is that your research
goals are likely to change. You need a solution that can be adapted as your needs evolve.
ÄKTA pure grows with your research
> Science needs protein purification to be easy
Regardless of your field of research, purified protein is often a necessity to accomplish your goals.
You need a solution that helps you spend less time on purifying proteins and more time focusing on the results.
ÄKTA pure lets you focus on your research
> In a competitive world, my chromatography system should just do the job
Research has never been as competitive as it is today. Protein purification is often one step of many in a much
larger research process. You need a solution you can rely upon to produce your pure proteins in time for when
they are needed.
ÄKTA pure builds on more than 50 years of protein purification expertise
There’s an ÄKTA pure for everyone Discover yours
Pure convenience
I need to get onwith my research
Pure cooperation
We’re all working on different projects
In our lab, we have to share the same chromatography system. None of us are purifying lots of protein, but we all need to do it at times. We need a system that’s easy to share and maintain together.
I develop assays that need pure proteins. I am not really comfortable purifying proteins, so I need a system that’s easy to work with.
For local office contact information, visit:
www.gelifesciences.com/contact
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
www.gelifesciences.com/aktapure
GE, imagination at work and GE monogram
are trademarks of General Electric Company.
ÄKTA and UNICORN are trademarks of GE
Healthcare companies.
© 2012 General Electric Company
All rights reserved.
First published Aug. 2012
GE Healthcare UK Limited
Amersham Place
Little Chalfont
Buckinghamshire, HP7 9NA
UK
GE Healthcare Europe, GmbH
Munzinger Strasse 5
D-79111 Freiburg
Germany
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327
Piscataway, NJ 08855-1327
USA
GE Healthcare Japan Corporation
Sanken Bldg., 3-25-1
Hyakunincho Shinjuku-ku
Tokyo 169-0073
Japan
Reverse-flow elution increases sample concentration Bypass for automation of cleaning Detection of standard proteins at 280 nm
Four inlet positions enable automatic system and column cleaning while running overnight
Fraction collection in tubes
We explore new proteins and want to learn more about their function. The most interesting proteins are often really difficult to purify. But has breaking new ground ever been easy? I need an efficient system to help me explore.
We purify mostly antibodies for our discovery programs, but also some drug targets and endogenous proteins. We are always looking to deliver more and keep costs down. What we need is more automation.
Pure automation
We need more efficiency
Pure exploration
Getting my proteins as pure as I need them is a real challenge
Completely load multiple samples automatically
Connect up to five columns, with automatic switching among them, with integrated pressure sensors for column protection
Detect any biomolecule with triple wavelength UV detection
Select multiple buffers and wash solutions with 14 inletswith integrated air sensors for system protection
Control gradients and elution conditions with pH and conductivity monitors
Use tubes or plates for flexible, high capacity fraction collection
ÄKTA™ pureProtein purification your way
GE HealthcareLife Sciences
> Intuitive and easy to use
Whether you are an expert or an absolute beginner in protein purification, ÄKTA pure makes routine protein purification easy.
The flow path is forward facing and organi-zed so that it is easy to understand how buffers and samples flow.
Convenient valves enable basic automation such as running overnight and including automatic cleaning steps.
> Fast method set-up
ÄKTA pure works with any column, but if you use one of ours, the method to run it is already defined in UNICORNTM 6 software.
If you would like to customize your method, it is easily achieved using the drag-and-drop interface.
For direct and full control of your runs, the software provides a user-friendly, interactive process picture.
> Add functionality when you need it
What if your next project takes an unexpec-ted turn to, for example, require the use of more automation or the use of a special detection method?
Relax. With the flexibility provided by ÄKTA pure in terms of valves, detectors, pumps, and software you can add almost any functionality you need to overcome any purification challenge.
If you need to run at low temperature, ÄKTA pure is small enough to fit inside a standard cold cabinet.
> Go quickly from one purification task to the next
With ÄKTA pure, turnaround from one purification task to another can be done in only a few minutes.
With a bypass function for your column and with multiple buffer inlet positions, cleaning can be automated to make sure the system is ready-to-use immediately after a run.
Changing sample application method from, for example, direct loading to injection from a loop is now simply done by a few clicks of a mouse due to the convenient injection valve.
> UNICORN on every computer
Because UNICORN 6 licenses are for concur-rent users, you can to freely install and access the software on as many computers as you like.
Add licenses as your usage of the software increases. Further, results and methods are easily shared within the team via the UNICORN central database.
The optional UNICORN column logbook keeps track of column performance and important run data, providing traceability and increased operational security, especially if columns are shared.
> Convenient, prepacked columns
We provide a vast selection of columns prepacked with our modern chromatogra-phy media providing solutions for conve-nient purification of tagged proteins and antibodies, but also gel filtration, IEX and HIC techniques, to match your varying needs for speed, purity, and yield.
The so called “crude concept” column design simplifies your workflow by enabling purification directly from crude, homogeni-zed, unclarified cell lysate.
> Automate the task
With ÄKTA pure, automation possibilities are endless—multiple buffer inlet positions, safe loading of up to seven samples completely, the ability to automatically switch between up to five columns, and flexibility to allow automatic two-step purification.
Add to that a fraction collector that can collect in tubes as well as microplates and ÄKTA pure will fit any purification workflow.
> From one system to a whole facility
UNICORN is a software platform that is common to ÄKTA systems from research- to process scale. The software can run in a network such that methods and data can be shared between all users and systems.
Sharing provides full flexibility and enables optimal use of available systems.
With UNICORN software and ÄKTA pure systems running in a network, increasing capacity by adding more systems is easy.
> Run unattended with confidence
ÄKTA pure automation valves have been designed to maximize your productivity.
Failures are eliminated with inlets safeguar-ded by integrated air sensors.
Columns are protected from overpressure by integrated pressure sensors controlling the flow.
Should a system alarm go off or if you simply want to know when the run is completed, UNICORN 6 can automatically send an e-mail notification so that you can take immediate action.
> Screening for optimal conditions made easy
Screening for optimal chromatography conditions is an important first step to maximize protein purity.
ÄKTA pure column selection valve allows automatic screening on multiple columns.
The loop valve enables, for example, scree-ning using the same sample in multiple conditions, and multiple buffer inlets can be used to screen for optimal buffer conditions.
> Detection to verify and evaluate any method
Several monitors to suit your application can be fitted to ÄKTA pure.
Up to two UV monitors can be combined. The pH valve in combination with the conducti-vity monitor gives you full verification of elution conditions.
Further, easy connection of external monitors for, for example, fluorescence, refractive index, and light scattering detec-tion applications greatly expands your possibilities.
> Integrated Design of Experiments (DoE)
DoE provides a structured approach to method optimization.
Using this optional feature in UNICORN 6 software, it is not only possible to plan an experimental series to maximize the amount of information obtained from a minimum number of experiments; the system can also run it in an automated fashion.
Once the experiment has been run, the results are used to create statistical models to determine the optimal chromatography conditions to be used going forward.
> Reality requires a chromatography solution that can be tailored to new needs
Whether your operation is small or large, one of the things that you know for sure is that your research
goals are likely to change. You need a solution that can be adapted as your needs evolve.
ÄKTA pure grows with your research
> Science needs protein purification to be easy
Regardless of your field of research, purified protein is often a necessity to accomplish your goals.
You need a solution that helps you spend less time on purifying proteins and more time focusing on the results.
ÄKTA pure lets you focus on your research
> In a competitive world, my chromatography system should just do the job
Research has never been as competitive as it is today. Protein purification is often one step of many in a much
larger research process. You need a solution you can rely upon to produce your pure proteins in time for when
they are needed.
ÄKTA pure builds on more than 50 years of protein purification expertise
There’s an ÄKTA pure for everyone Discover yours
Pure convenience
I need to get onwith my research
Pure cooperation
We’re all working on different projects
In our lab, we have to share the same chromatography system. None of us are purifying lots of protein, but we all need to do it at times. We need a system that’s easy to share and maintain together.
I develop assays that need pure proteins. I am not really comfortable purifying proteins, so I need a system that’s easy to work with.
For local office contact information, visit:
www.gelifesciences.com/contact
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
www.gelifesciences.com/aktapure
GE, imagination at work and GE monogram
are trademarks of General Electric Company.
ÄKTA and UNICORN are trademarks of GE
Healthcare companies.
© 2012 General Electric Company
All rights reserved.
First published Aug. 2012
GE Healthcare UK Limited
Amersham Place
Little Chalfont
Buckinghamshire, HP7 9NA
UK
GE Healthcare Europe, GmbH
Munzinger Strasse 5
D-79111 Freiburg
Germany
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327
Piscataway, NJ 08855-1327
USA
GE Healthcare Japan Corporation
Sanken Bldg., 3-25-1
Hyakunincho Shinjuku-ku
Tokyo 169-0073
Japan
Reverse-flow elution increases sample concentration Bypass for automation of cleaning Detection of standard proteins at 280 nm
Four inlet positions enable automatic system and column cleaning while running overnight
Fraction collection in tubes
We explore new proteins and want to learn more about their function. The most interesting proteins are often really difficult to purify. But has breaking new ground ever been easy? I need an efficient system to help me explore.
We purify mostly antibodies for our discovery programs, but also some drug targets and endogenous proteins. We are always looking to deliver more and keep costs down. What we need is more automation.
Pure automation
We need more efficiency
Pure exploration
Getting my proteins as pure as I need them is a real challenge
Completely load multiple samples automatically
Connect up to five columns, with automatic switching among them, with integrated pressure sensors for column protection
Detect any biomolecule with triple wavelength UV detection
Select multiple buffers and wash solutions with 14 inletswith integrated air sensors for system protection
Control gradients and elution conditions with pH and conductivity monitors
Use tubes or plates for flexible, high capacity fraction collection
ÄKTA™ pureProtein purification your way
GE HealthcareLife Sciences
> Intuitive and easy to use
Whether you are an expert or an absolute beginner in protein purification, ÄKTA pure makes routine protein purification easy.
The flow path is forward facing and organi-zed so that it is easy to understand how buffers and samples flow.
Convenient valves enable basic automation such as running overnight and including automatic cleaning steps.
> Fast method set-up
ÄKTA pure works with any column, but if you use one of ours, the method to run it is already defined in UNICORNTM 6 software.
If you would like to customize your method, it is easily achieved using the drag-and-drop interface.
For direct and full control of your runs, the software provides a user-friendly, interactive process picture.
> Add functionality when you need it
What if your next project takes an unexpec-ted turn to, for example, require the use of more automation or the use of a special detection method?
Relax. With the flexibility provided by ÄKTA pure in terms of valves, detectors, pumps, and software you can add almost any functionality you need to overcome any purification challenge.
If you need to run at low temperature, ÄKTA pure is small enough to fit inside a standard cold cabinet.
> Go quickly from one purification task to the next
With ÄKTA pure, turnaround from one purification task to another can be done in only a few minutes.
With a bypass function for your column and with multiple buffer inlet positions, cleaning can be automated to make sure the system is ready-to-use immediately after a run.
Changing sample application method from, for example, direct loading to injection from a loop is now simply done by a few clicks of a mouse due to the convenient injection valve.
> UNICORN on every computer
Because UNICORN 6 licenses are for concur-rent users, you can to freely install and access the software on as many computers as you like.
Add licenses as your usage of the software increases. Further, results and methods are easily shared within the team via the UNICORN central database.
The optional UNICORN column logbook keeps track of column performance and important run data, providing traceability and increased operational security, especially if columns are shared.
> Convenient, prepacked columns
We provide a vast selection of columns prepacked with our modern chromatogra-phy media providing solutions for conve-nient purification of tagged proteins and antibodies, but also gel filtration, IEX and HIC techniques, to match your varying needs for speed, purity, and yield.
The so called “crude concept” column design simplifies your workflow by enabling purification directly from crude, homogeni-zed, unclarified cell lysate.
> Automate the task
With ÄKTA pure, automation possibilities are endless—multiple buffer inlet positions, safe loading of up to seven samples completely, the ability to automatically switch between up to five columns, and flexibility to allow automatic two-step purification.
Add to that a fraction collector that can collect in tubes as well as microplates and ÄKTA pure will fit any purification workflow.
> From one system to a whole facility
UNICORN is a software platform that is common to ÄKTA systems from research- to process scale. The software can run in a network such that methods and data can be shared between all users and systems.
Sharing provides full flexibility and enables optimal use of available systems.
With UNICORN software and ÄKTA pure systems running in a network, increasing capacity by adding more systems is easy.
> Run unattended with confidence
ÄKTA pure automation valves have been designed to maximize your productivity.
Failures are eliminated with inlets safeguar-ded by integrated air sensors.
Columns are protected from overpressure by integrated pressure sensors controlling the flow.
Should a system alarm go off or if you simply want to know when the run is completed, UNICORN 6 can automatically send an e-mail notification so that you can take immediate action.
> Screening for optimal conditions made easy
Screening for optimal chromatography conditions is an important first step to maximize protein purity.
ÄKTA pure column selection valve allows automatic screening on multiple columns.
The loop valve enables, for example, scree-ning using the same sample in multiple conditions, and multiple buffer inlets can be used to screen for optimal buffer conditions.
> Detection to verify and evaluate any method
Several monitors to suit your application can be fitted to ÄKTA pure.
Up to two UV monitors can be combined. The pH valve in combination with the conducti-vity monitor gives you full verification of elution conditions.
Further, easy connection of external monitors for, for example, fluorescence, refractive index, and light scattering detec-tion applications greatly expands your possibilities.
> Integrated Design of Experiments (DoE)
DoE provides a structured approach to method optimization.
Using this optional feature in UNICORN 6 software, it is not only possible to plan an experimental series to maximize the amount of information obtained from a minimum number of experiments; the system can also run it in an automated fashion.
Once the experiment has been run, the results are used to create statistical models to determine the optimal chromatography conditions to be used going forward.