PROTOZOARE

Asistent universitar NEAGOE IONELA

Medicina generala – UMF “Carol Davila”

AMIBE

AMIBE PATOGENE

Entamoeba hystolitica

Entamoeba hystolitica Stadiul de CHIST

Examen coproparazitologic = Frotiu din fecale – coloratie Tricrom

Entamoeba hystolitica Stadiul de TROFOZOIT Trophe (gr)=

hranire; zoon

(gr)= animal

Examen

coproparazitologic =

Frotiu din fecale

muco-pio-

sangvinolente –

coloratie Giemsa

Entamoeba hystolitica Colonoscopie – Abcese primare in “buton de camasa”

Entamoeba hystolitica Abces primar in “buton de camasa”

Examen histopatologic = Sectiune tesut intestinal – coloratie H&E

Entamoeba hystolitica Abces secundar amoebian - hepatic

Entamoeba hystolitica Abces secundar amoebian -perianal

AMIBE COMENSALE

Entamoeba coli Stadiul de CHIST

Examen

coproparazitologic

= Preparat proaspat

L+l din fecale +

solutie Lugol

8 nuclei in

planuri

suprapuse

AMIBE LIBERE CONDITIONAT

PATOGENE

1. Naegleria fowlery

2. Acanthamoeba sp.

Naegleria fowlery

Naegleria fowlery Stadiul de CHIST

Naegleria fowlery Stadiul FLAGELAT

Flagel

(2)

Naegleria fowlery

Examen histopatologic =

Sectiune tesut cerebral

coloratie H&E

Stadiul de TROFOZOIT

Recunoastere dupa nucleul cu aspect de tinta

Acanthamoeba sp.

Acanthamoeba sp. Stadiul de CHIST

Preparat

proaspat

L+l din

suspensie

tesut de

raclaj

corneean

Acanthamoeba sp. Stadiul de TROFOZOIT

Nucleu

Acantopodii

Preparat

proaspat

L+l din

LCR

Acanthamoeba sp. Keratita amoebiana

Acanthamoeba sp. Abcese subcutanate granulomatoase (la imunosupresati)

Encefalita granulomatoasa amoebiana cronica (la imunosupresati)

Acanthamoeba sp.

FLAGELATE

FLAGELATE CAVITARE

1. Giardia duodenalis

2. Trichomonas vaginalis

Giardia duodenalis

Giardia duodenalis

Examen

coproparazitologic

= Preparat proaspat

L+l din fecale formate

+ SF

Stadiul de CHIST

Giardia duodenalis Stadiul de TROFOZOIT

Examen

coproparazitolo

gic =

Frotiu fecale

diareice –

coloratie Giemsa

Giardia duodenalis

Instalare MALABSORTII

Ventuza/Disc adeziv

Stadiul de TROFOZOIT

Trichomonas vaginalis

Trichomonas vaginalis Stadiul de TROFOZOIT

Frotiu secretie – coloratie Giemsa

Trichomonas vaginalis Coposcopie – Vaginita + Leucoree – FAZA INCIPIENTA

Trichomonas vaginalis Colposcopie – “Strawberry cervix” + coinfectie cu Candida

Trichomonas vaginalis

Testul

InPouch

TV pentru

detectia

directa si

cultivarea

trofozoitilor

Test rapid imunocromatografic – detectie antigene

Trichomonas vaginalis

HEMOFLAGELATE

1. Leishmania sp.

2. Trypanosoma brucei

3. Trypanosoma cruzii

Leishmania sp.

Leishmania sp.

Lutzomia sp. Phlebotomus sp

(Sandfly)

kinetoplast

Flagel liber

nucleu

Leishmania sp.

Stadiul PROMASTOGOT

Frotiu GLANDE

SALIVARE

MUSCULITA DE

NISIP - coloratie

GIEMSA

Mastigot =

kinetida (gr) =

flagel+

corpuscul

bazal/kinetosom

Leishmania sp. Stadiul AMASTOGOT

Amastigot uninucleat

kinetoplast

Frotiu aspirat

medular –

coloratie

Giemsa

Macrofag

Leishmania sp. Buton de Orient

- Forma uscata - L. tropica

Leishmania sp. Buton Orient - Forma umeda – L. major

Leishmania sp. Forma ESPUNDIA – L. brasiliensis

Leishmania sp. Ulcerul chiclero- L. mexicana

Leishmania sp. Boala Kala-azar, febra Dum-Dum, boala neagra – FAZA CRONICA

L. donovani

L. Infantum

L. chagasi

Leishmania sp.

Leishmanioza cutanata post kala-azar – FAZA CRONICA

Leismania donovani

Trypanosoma brucei

Trypanosoma brucei Glossina sp. ( musca tze-tze )

Trypanosoma brucei Stadiul EPIMASTOGOT

Frotiu din

cultura

dezvoltata

pe mediul

NNN -

coloratie

GIEMSA

Nucleu

Membrana ondulanta

scurta

Flagel

liber

anterior

Trypanosoma brucei Stadiul TRIPOMASTIGOT

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Membrana

ondulanta lunga

Corpuscul bazal /Kinetosom

Kinetoplast

Nucleu

Flagel liber

anterior

Trypanosoma brucei gambiense Sleeping sickness (Boala somnului) – FAZA INCIPIENTA

Semnul Winterbottom

Trypanosoma brucei Sleeping sickness (Boala somnului) – FAZA CRONICA

Meningoencefalita cronica

Trypanosoma cruzii

Trypanosoma cruzii

Triatoma sp. (Kissing bug)

Reduviid bug

Trypanosoma cruzii

Stadiul TRIPOMASTIGOT

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Trypanosoma cruzii Stadiul AMASTOGOT

Amastogoti

uninucleati

Celula

nervoasa

Aprenta tesut cardiac –

coloratie Giemsa

Trypanosoma cruzii

Semnul Romana

Boala Chagasi (FAZA INCIPIENTA)

Trypanosoma cruzii Boala Chagasi (FAZA CRONICA)

Anevrism

cardiac

Cardiomiopatie cu

megalie cardiaca

Trypanosoma cruzii Boala Chagasi (FAZA CRONICA)

Megacolon

SPOROZOARE

1. Toxoplasma gondii

Sporozoare tisulare

Toxoplasma gondii

Toxoplasma gondii Stadiul de OOCHIST

Examen coproparazitologic – fecale PISICA

P.C.

Sporochisti

Sporozoiti

uninucleati

Toxoplasma gondii Stadiul de TACHIZOIT (trofozoitul de Toxoplasma)

TACHIZOITI uninucleati

Macrofag

tachys (gr)=rapid; zoon (gr)= animal

Frotiu

ascita

soarece

infectat –

coloratie

Giemsa

Toxoplasma gondii Stadiul de CHIST TISULAR cu bradizoiti

Celula

nervoasa

BRADIZOITI

bradys (gr)=lent; zoon (gr)= animal

Amprenta

tesut

cerebral

coloratie

Giemsa

Toxoplasma gondii Neurotoxoplasmoza (la imunosupresati)

CT

Toxoplasma gondii Corioretinita

(la imunosupresati si la

nou-nascuti cu

toxoplasmoza

congenitala)

Toxoplasma gondii

Hidrocefalie

2. Plasmodium vivax

Plasmodium ovale

Plasmodium malariae

Plasmodium falciparum

Sporozoare tisulare

Plasmodium sp.

Plasmodium sp. Tantarul Anopheles sp.

Plasmodium sp. OOCHISTI fixati pe fata externa a stomacului unui tantar infectat.

Plasmodium sp. SPOROZOITI

Frotiu GLANDE SALIVARE TANTAR - coloratie GIEMSA

Plasmodium sp.

MEROZOITI INTRAHEPATOCITARI

Amprenta

tesut

hepatic

coloratie

Giemsa

Plasmodium sp. Plasmodium vivax

Nucleu

inel

Citoplasma

putina,

periferica

Vacuola

centrala

INEL= TROFOZOIT TANAR

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium vivax

Citoplasma

bogata, cu

multe

prelungiri Nucleu

amoeba

AMOEBA = TROFOZOIT MATUR

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium vivax

Nuclei

merozoiti

Granulatii

Schuffner

ROZETA = SCHIZONT MATUR 16-24 merozoiti

“Corp muriform”

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium vivax

GAMETOCIT MASCULIN

Nucleu

central

nedefinit

Granulatii

Schuffner

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium vivax

GAMETOCIT FEMININ

Nucleu gametocit -

periferic, triunghiular

Granulatii

Schuffner

Frotiu

SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium ovale

INEL= TROFOZOIT TANAR

Nucleu inel

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium ovale

Nucleu

amoeba

AMOEBA = TROFOZOIT MATUR

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium ovale

ROZETA = SCHIZONT MATUR 8-12 merozoiti

Nuclei

merozoiti

Frotiu

SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium ovale

Granulatii

Schuffner /

James

GAMETOCIT MASCULIN

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium ovale

Nucleu gametocit -

periferic, triunghiular

Granulatii

Schuffner /

James

GAMETOCIT FEMININ

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium malarie

INEL= TROFOZOIT TANAR

Nucleu

inel

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium malarie

Granulatii

Ziemann

Nucleu

amoeba

Amoeba “in banda”

AMOEBA = TROFOZOIT MATUR

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium malarie

Nuclei

granulocit

Nuclei

merozoiti

ROZETA = SCHIZONT MATUR 8-12 merozoiti

Rozeta “in margareta”

Granulatii

Ziemann

Examen

hematologic =

Picatura

groasa

SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium malarie

GAMETOCIT MASCULIN

Granulatii

Ziemann

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium malarie

GAMETOCIT FEMININ

Nucleu gametocit -

periferic, triunghiular

Granulatii

Ziemann

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium falciparum

INEL= TROFOZOIT TANAR

Inele uni si bi nucleate

Hematie

multiparazitata

cu inele

FAZA INCIPIENTA

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium falciparum

FAZA COMATOASA

AMOEBA = TROFOZOIT MATUR

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium falciparum

ROZETA = SCHIZONT MATUR 8-32 merozoiti

granulatii

Maurer

Merozoiti

uninucleati

FAZA COMATOASA

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium falciparum

GAMETOCIT MASCULIN “in cosulet”

granulatii

Maurer

Nucleu

gametocit

FAZA INCIPIENTA

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium sp. Plasmodium falciparum

GAMETOCIT FEMININ “in cosulet”

FAZA INCIPIENTA

Examen

hematologic =

Frotiu SANGE

PERIFERIC

coloratie

GIEMSA

Plasmodium falciparum Neuropaludism – Malarie cerebrala

Cryptosporidium sp.

3. Cryptosporidium parvum

Cryptosporidium hominis

Sporozoare digestive

Cryptosporidium sp. Stadiul de OOCHIST

Examen coproparazitologic = Frotiu fecale coloratie ZIEHL-NIELSEN modificata

Sporozoiti

uninucleati

(4)

4. Isospora belli

Sporozoar digestiv

Isospora belli

Isospora belli Stadiul de OOCHIST

Frotiu fecale coloratie

ZIEHL-NIELSEN

modificata

Preparat

proaspat L+ l

din fecale cu SF

Qochist

matur Qochist

imatur

Sporochist

Sporozoiti uninucleati

Examen coproparazitologic

5. Pneumocystis carinii

Pneumocystis jiroveci

Sporozoar - Transfug

Pneumocystis carinii Pneumocystis

jiroveci

Cale

aerogena

Pneumocystis carinii

TROFOZOITI (nuclei trofozoiti in “ nor cu puncte”)

Frotiu LBA coloratie GIEMSA.

Pneumocystis carinii

CHISTI

cu (8)

corpusculii

colorati

TROFOZOITI

Frotiu LBA coloratie GIEMSA.

Pneumocystis carinii

Frotiu LBA coloratie Albastru de Toluidina.

CHISTI cu perete

celular colorat

Pneumocystis carinii

RX- plamani in “geam mat”