PROTOZOA

The protozoa are contained within the kingdom Protista along with the unicellular algae. Theclasses of protozoa are categorized by a variety of factors: cell architecture, motility structure,even hosts. They do not photosynthesize, rather being chemoheterotrophic like animals.This means that they use chemicals for energy production and they get their carbon from thesame compounds, e.g. sugar. However, there are some pigmented protozoa and there areeven a few that seem to be crossover organisms, being claimed by the botanists because oftheir photosynthetic ability. for example, the flagellate Euglena will photosynthesize in light (itcontains chlorophyll) or will switch over to regular aerobic respiration (chemoheterotrophism)without light.

Many of the protozoa form a resistant, dormant structure called a cyst. Parasitic protozoaare identified by the active feeding stage, called a trophozoite, in addition to the cyst stage,both of which may be found in the feces.

For our purposes, there are only 4 groups of protozoa that will be covered here: thesegroups are separated by motility and cell structure.

Amebas (representative: Ameba proteus) Flagellates (representative: Trypanosoma, Euglena) Ciliates (representative: Paramecium) Apicomplexa (representative: Plasmodium)

Many protozoa are found in the gut of warm-blood animals and cold-blooded animals, as wellas in insects such as termites and cockroaches. In addition, there are quite a fewprotozoans that live in blood. You will see some of these examples in lab.

Amebas move by cytoplasmic streaming, having no motility structure. You will likely seesome freshwater amebas in the pond water, some of which may have tests or 'shells' thatsurround their cytoplasm. The flagellates have flagella or an undulating membrane formotility. The ciliates have cilia. The Apicomplexa have a unique arrangement ofmicrotubules, called the apical complex (used in the takeover of the host cell), in the cell.This last class has most of the human and animal pathogens in it.

EXTERNAL LINKS:

Pond Water Critters, Pond Water CrittersMolecular Expressions, http://micro.magnet.fsu.edu/moviegallery/pondscum.html - excellentmovies

Ameba

Paramecium

Plasmodium Trypanosoma

OBJECTIVES:

Identify common species of protozoa.Differentiate among the major classes of protozoa.Identify different types of motility.

MATERIALS NEEDED:

prepared slides: Trypanosoma and Plasmodiumfresh specimens: Ameba and Parameciumpond water

PROCEDURE:

1. Make wet mounts of the pond water.

Go down to the bottom of the container or in the gunk to get your specimen. Do NOT stir the specimen: you will get fewer that way. Start with the 10X and go to 40X. Oil-immersion will magnify too much for most pond

water protozoa. Once you have found your objects on brightfield, change over to darkfield and phase-

contrast for even better viewing.

2. Look at the READY VIEW (also called DEMOSLIDE) slide/tubes containing Parameciumand Ameba. a use culture tube/slide for studying microscopic organisms. It is a taperedtube with a compressed viewing chamber at its tip, so you can just place the tube into aholder slide that fits right on the microscope stage. The protozoa will be at the bottom of thetube, making it very easy to focus on them. The optimal magnification for these organisms is10X, maybe up to 40X. You can use both brightfield microscopy as well as phase-contrastmicroscopy (be sure to use the appropriate phase condenser setting)

3. Look at the prepared blood slides of Trypanosoma and Plasmodium on 100X, usingbrightfield microscopy. Trypanosoma will be easy to see: it is far larger than the red bloodcells. However, Plasmodium will be difficult since the parasite will be inside of the RBCs.

QUESTIONS:

1. Where is the malarial parasite located---in the RBC or in the plasma outside of the RBC?

2. By what major criterion are the protozoa subcategorized into classes?

Fall 2011, Jackie Reynolds, Richland College, BIOL 2420