Proteomics & Metabolomics
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Proteomics and Metabolomics Shared Resource (PMSR) Stephen Byers, PhD, Co-Director Al Fornace, MD, Co-Director Amrita Cheema, PhD, Metabolomics [email protected] Rado Goldman, Proteomics [email protected]
Transcript of Proteomics & Metabolomics
Proteomics and Metabolomics Shared Resource (PMSR)Stephen Byers, PhD, Co-Director
Al Fornace, MD, Co-Director
Amrita Cheema, PhD, Metabolomics [email protected] Goldman, [email protected]
Proteomics: An Integrated Approach
Gel Based Workflow
LC Based Workflow
Integrated Proteomics
2D Gel Workflow
DIGE Imager
2D DIGE
ECL Plex Western Blot
Applications: • Multiplexing of gels and visualization of differentially expressed spots between the sample and the control on the same gel.• Differential Protein analysis: using the De Cyder Software.• Improved spot detection: due to high sensitivity of the fluorescent labeling.• Imager: used for other fluorescent imaging applications.
Spot Picker
Robotic spot picker:
•picks spots from 2D gels with increased accuracy
•efficiency based on the protein pick list generated by the image analysis software.
MALDI TOF-TOF Mass Spectrometers
Applications:
•Identification of Proteins from complex biological samples using MASCOT.•Supports LC MALDI workflows for isobariclabeling of proteins followed by identification.•Serum Biomarker studies.•In-Silico digest workflows for the analysis of post translational modifications.
Nano LC coupled with
Probotic Spotter
4800 Proteomics Analyzer
Nano Acquity UPLC- QSTAR ELITE LCMS/MS system
•Optimized for high throughput‘Shot gun’ proteomics workflowsin conjunction with the online nano flow TEMPO MDLC system.
•High Speed data acquisition, database searching and processing Software.
•Ideally suited for quantitative proteomics using iTRAQ/ ICAT labeling methods for differential proteomic profiling
MALDI TOF-TOF Mass Spectrometers
•Ideally suited for quantitative proteomics using iTRAQ/ ICAT labeling methods for differential proteomic profiling.
•Detection of Post translational modifications.
•Serum Profiling studies made easy with marker view softwares for Principle component analysis.
•Metabolomics and metabolite detection.
•Detection and Identification of low abundance proteins due to high sensitivity and mass accuracy.
What Responses are Possible?
UPLC-TOFMS
Applications:•Small molecule profiling•Small molecule quantization
Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01
In your publications, remember to mention support provided from the GHUCCTS.
