Protein Targeting.

Presented by:-

Ajay kr. Chandra

BT/02/09 5th semester

•What is Protein Targeting?

•Why protein is targeted?

•Where it occurs?

signal sequences • First by Gunter Blobel (1970) ,won the Nobel Prize (1999) for his

work on protein targeting.• Directs a protein to its appropriate location after synthesis.• Signals vary from 13 to 36 amino acid residues. a. About 10 to 15 hydrophobic amino acid residues. b. One or more positively charged residues near N-terminus. c. A short cleavage sequence often including(polar A.A) Ala.

Posttranslational Modification of Eukaryotic Proteins Begins in the ER.

Glycosylation Plays a Key Role in Protein Targeting.

Golgybody & Lysosomal targeting.

Phosphorylation of a mannose residue on lysosome-targeted enzymes.

Proteins are Targeted to Mitochondria and Chloroplasts by Similar Pathways.

Nuclear Targeting.

Bacteria Also use Signal Sequences for protein targeting.

Model for protein export in bacteria.

Cells Import Proteins by Receptor-mediated Endocytosis.

clathrin

Summary• Protein targeting is necessary for proteins that are

destined to work outside the cytoplasm. • Requires sorting signals that direct their transport from the

cytosol. • Correct sorting is crucial for the cell; errors can lead to

diseases.• Protein targeting is more complex in eukaryotes because of

the presence of many intracellular compartments.• targeting either of two ways depending on the protein …. 1) Co translational translocation . 2) Posttranslational translocation .

References• Wikipedia… www.google.co.in.• LEHNINGER :- principles of Biochemistry ; Cox and Nelson ;

5th edition ; page no.= 1100-1107.• Molecular cell Biology:- Lodish and Berk et al ; 5th edition ;

page no.= 657-739.

Thanks