Protein Engineering

BIT317 Class 2438

Lecture 12Protein Structure

Motifs (supersecondary structures) in proteins

Greek keyBeta hairpin

Beta-omega-betaRossman fold

All Alpha proteins

Three helix bundle

Globin fold (8 helices)

All Beta Sheet Proteins

Beta Barrel(ion channels) Beta Propellor

(light harvesting complexes)

Alpha / beta structures

Rossman Fold(beta-alpha-beta-alpha-beta)TIM Barrel (alpha/beta)8

Alpha + beta structures

Ferridoxin Fold DNA Clamp

Non-covalent interactions in proteins

Intramolecular interactions Hydrogen bonding Ion pair interactions (salt bridges) Cation-pi interactions Pi-pi interactions Hydrophobic interactions

Intermolecular interactions Solvation / hydration

Specific examples for the above should be learnt as discussed in the class

Non-covalent interactions in proteins