Protein Metrics Solutions
15
Bispecific Antibodies Bispecific Antibody Possess the characteristics and specificity of two distinct monoclonal antibodies
Transcript of Protein Metrics Solutions
Bispecific Antibodies
Bispecific AntibodyPossess the characteristics and specificity
of two distinct monoclonal antibodies
What is a Bispecific Antibody?
Dillon & Yin (2016) MABS 9: 213-230Schachner & Han (2016) Anal. Chem. 88: 12122-12127
RECRUIT cytotoxicT-cells or NK-cells
ACTIVATE receptor
• Possess the characteristics and specificity of two distinct monoclonal
antibodies
• Can bind to two different targets simultaneously
• >50 molecules currently in clinical development
• Complex multi-chain molecules that can be challenging to produce in
sufficient quantity and quality
• Bispecific antibody formats based on IgG preferred for many applications
• Easy purification
• Long serum half-life
• Effector functions
Wilson Phung, Genentech (Webcast Sept 2017)(https://www.technologynetworks.com/tn/webinars/high-throughput-quantitative-analysis-of-bispecific-antibody-moieties-and-glycosylation-290703)
What is a Bispecific Antibody?
Dillon & Yin (2016) MABS 9: 213-230Schachner & Han (2016) Anal. Chem. 88: 12122-12127
DELIVERY acrossblood brain barrier
BLOCK two independentligands or receptors
• Possess the characteristics and specificity of two distinct monoclonal
antibodies
– Can bind to two different targets simultaneously
• >50 molecules currently in clinical development
• Complex multi-chain molecules that can be challenging to produce in
sufficient quantity and quality
• Bispecific antibody formats based on IgG preferred for many applications
– Easy purification
– Long serum half-life
– Effector functions
Wilson Phung, Genentech (Webcast Sept 2017)(https://www.technologynetworks.com/tn/webinars/high-throughput-quantitative-analysis-of-bispecific-antibody-moieties-and-glycosylation-290703)
Alternative Formats for Bispecific Antibodies
Technical Hurdles to Bispecific Production
+ + +
Single Cell Production – in vivo assembly
Unwanted IgG speciesDesired Bispecific
Problems to solve:
1. Unwanted heavy chain homodimerization
2. Unwanted light chain – heavy chain pairing
Bi-Specific webinar with Genentech
• Moving data processing to the next level of efficiency
• Software to quickly identify and report desired, undesired and unknown species
• High throughput capabilities
https://www.technologynetworks.com/tn/webinars/high-throughput-quantitative-analysis-of-bispecific-antibody-moieties-and-glycosylation-290703
Workflow Automation - Bispecifics
Identification of desired forms
INCORRECT
Identification of undesired forms
Antibody Drug Conjugates (ADC)
ADCs
Drug loading distribution (by sample)
DAR values (Automatically calculated)
ADC webinar StemCentrx (BRK and PMI)
• Ability to use ALL of the data, no matter how complex
• Bruker data on Antibody-Drug-Conjugates (ADCs) in a StemCentrx webinar shows complex data
• Need to calculate ‘Drug-to-Antibody Ratio’ (DAR)
• StemCentrx, Bruker and Protein Metrics worked to improve the workflow in a real-world environment with HIGHLY COMPLEX data
http://worldadc-webinars.com/view-webinars/webinar-archive/
