PROTEIN BASED NANO MACHINES FOR SPACE APPLICATIONS · 2012-10-10 · PROTEIN BASED NANO MACHINES...
Transcript of PROTEIN BASED NANO MACHINES FOR SPACE APPLICATIONS · 2012-10-10 · PROTEIN BASED NANO MACHINES...
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PROTEIN BASED NANO MACHINES FOR SPACE
APPLICATIONS
Dr. Constantinos Mavroidis, Associate Professor
Department of Mechanical and Aerospace Engineering
Rutgers, The State University of New JerseyNIAC Phase I GrantNIAC Phase I Grant
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THE TEAM
Dr. C. MavroidisAssociate Professor Mechanical Engineering, Rutgers University
Dr. M. YarmushChair, Biomedical Engineering, Rutgers University
Dr. M. S. TomassoneAssistant Professor, Biochemical Engineering Rutgers University
Dr. F. PapadimitrakopoulosAssociate Professor Department of Chemistry University of Connecticut
Dr. B. YurkeResearcher, Bell Laboratories, Lucent Technologies Inc.
Mr. Atul DubeyGraduate Student Rutgers University
Ms. Angela ThorntonGraduate Student Rutgers University
Mr. Kevin NikitczukUndergraduate Student Rutgers University
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OUR VISIONTo Develop Protein Based To Develop Protein Based NanoNano Machines and RobotsMachines and Robots
NovelNovelBiologicalBiologicalMultiMulti--Degree of FreedomDegree of FreedomApplyApply Forces Forces Manipulate Objects Manipulate Objects Move From Move From NanoNano to Macroto MacroLightweight / EfficientLightweight / EfficientSelfSelf--AssemblingAssemblingSelfSelf--ReproducingReproducing
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APPLICATIONS
Outer Outer SpaceSpace and Planetary Missionsand Planetary Missions
Colonization
Workstations
Military
Medical
Manufacturing
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APPLICATIONS
Bio-Nano-Robot Repairing a Damaged Blood Cell
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0-10 YEARS: DEVELOPMENT OF BIO NANO COMPONENTS
DNA VPL Motor Bacteriorhodopsin
DNA – Structural Member, Power Source
VPL – Protein Based Actuator
Bacteriorhodopsin, HSF – Nano Sensors
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MACRO-NANO EQUIVALENCEStructural Elements
Metal, Plastic Polymer DNA, Nanotubes
Power Sources
Electric Motors, Pneumatic Actuators, Smart Materials, Batteries, etc.
ATPase, VPL Motor, DNA
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MACRO-NANO EQUIVALENCE
Compliance DevicesSprings
Various Types of Gears, Belts, Chains etc.
VPL Platforms, DNA Double Crossover Molecules
β-Sheets
Transmission Elements
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MACRO-NANO EQUIVALENCE
Sensors
Light sensors, force sensors, position sensors, temperature sensors
Actuated Joints
Revolute, Prismatic, Spherical Joints etc.
DNA Nanodevices,Nanojoints
Rhodopsin, Heat Shock Factor
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10-20 YRS: NANOROBOTIC ASSEMBLIES
ATPase Motor Propelled
Structure – Nanotubes
Legs – Helical Proteins
Vision of a Nano Robot
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20-30 YRS – SELF SUSTAINMENT AND REPLICATION
Self Replication SustainmentSwarm IntelligenceControllability
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30-50 YRS – DEPLOYMENT FOR SPACE COLONIZATION
Courtesy: http://members.cox.net/kableguy/bryceworks/
Space Colonization
Non-living Robots
Bio Mimetic
Remote Sensing
Signal Transmission
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SPECIFIC AIMS FOR PHASE I
Identify Proteins for Use in Nanoscale MechanismsIdentify Proteins for Use in Nanoscale Mechanisms
Develop Concepts for Bio Develop Concepts for Bio NanoNano Machine componentsMachine components
Develop Dynamic Models and Realistic SimulationsDevelop Dynamic Models and Realistic Simulations
Perform a Series of Biomolecular ExperimentsPerform a Series of Biomolecular Experiments
Assembly and Interface Assembly and Interface NanoNano Machine ComponentsMachine Components
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VPL MOTOR CONCEPT
Viral Membrane Peptides
pH Dependent
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VPL ACTUATED PLATFORMS
Viral Protein Linear Motor Actuated Parallel Platforms with Controllable Motion
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VPL OUTPUT MULTIPLICATION
VPL Motors in Parallel –Force Multiplication
VPL Motors in Series –Displacement Multiplication
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BIOSENSOR SYSTEM
HSF Protein in Organisms
Responds to Stimuli – Trimerises
Binds to DNA
Color Change
Signal Transmission
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MULTI- DOF DEVICES
3 VPL Actuators
Nanotubes
DNA Joints
Response to pH Changes
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COMPUTATIONAL STUDIESModel Reversible Folding of VPL Motor Protein
Estimate Forces, Displacements etc. Through Energy
Software Usage - CHARMM
Input – Structure Files in .pdb Format
Output – Simulated Energy and Displacements
Microsecond Modeling – Assumptions, Targeted MD
Parallel Processing Facilities at CAIP (Teal)
Comparison with Experimental Observations
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EXPERIMENTAL WORK
Peptide Selection
Protein ExpressionProtein PurificationProtein Conformation as a Function of pH Calculate Force Expended upon ExtensionReversibilityDifferent Sequence - Different Designs
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WEBPAGE
http://bionano.rutgers.edu
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OUTREACH ACTIVITIES
High School Students in Research
Minority Students in Research
Undergraduate Students Employed
Technology Transfer
International and Industry Collaboration
Colloquiums, Symposia and Journal Clubs
Interdepartmental Course on Bio Nano Technology
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ACKNOWLEDGEMENTS
NASA Institute of Advanced Concepts (NIAC)SROA Program and Rutgers University, NJNSF Nanomanufacturing Program