PROSPECT OF THE ENTOMOPATHOGENIC NEMATODES AS BIOLOGICAL CONTROL FOR THE FUTURE IN INDONESIA AND...

NARESUAN UNIVERSITY-PHITSANULOK- 29 JULI 2010NARESUAN UNIVERSITY-PHITSANULOK- 29 JULI 2010

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Prof. Dr. Ir. Didik Sulistyanto

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PROSPECT OF THE ENTOMOPATHOGENIC

NEMATODES AS BIOLOGICAL CONTROL FOR THE FUTURE IN

ASEAN COUNTRIES

Prof.Dr. sc. agr. Ir. Didik Sulistyanto

Indonesia2013


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• Entomopathogenic nematodes have emerged as Entomopathogenic nematodes have emerged as excellent biological control agents for insect pestsexcellent biological control agents for insect pests,,

• Broad host range, safety to non-target organisms and Broad host range, safety to non-target organisms and the environment,the environment,

• Exemption from registration in many countries, ease Exemption from registration in many countries, ease of mass production and application, ability to search of mass production and application, ability to search for pests, for pests,

• Rapid host mortality, potential to recycle in the Rapid host mortality, potential to recycle in the environment and capability with most agricultural environment and capability with most agricultural chemicals,chemicals,

INTRODUCTION


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• Entomopathogenic nematodes as biological control Entomopathogenic nematodes as biological control agents for soil-dwelling stage of many insect pests,agents for soil-dwelling stage of many insect pests,

• Progress EPNs available commercially for large scale Progress EPNs available commercially for large scale application in plantation, horticultureapplication in plantation, horticulture,, turf grass, rice turf grass, rice plantation, fruit plantation, coffee, cacao, sugarcane, plantation, fruit plantation, coffee, cacao, sugarcane, etc,…etc,…

• SteinernemaSteinernema sp. sp. andand HeterorhabditisHeterorhabditis sp. isolat sp. isolat effective as biological control insect pests and ease effective as biological control insect pests and ease to mass produce to mass produce in vivo in vivo and and in vitro in vitro culture,culture,

PENDAHULUAN


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POTENTIAL OF ENTOMOPATHOGENIC NEMATODESPOTENTIAL OF ENTOMOPATHOGENIC NEMATODES

Advantage:

1. Rapid host mortality 24-48 hour,

2. Broad host range, Safety to non-target organisms and environment,

3. Ease of mass production in vitro and in vivo,

4. Ease to application in the field,

5. Compatibility with most agricultural chemicals,

6. Available commercially for large-scale application in plantation and others,


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ENTOMOPATHOGENIC NEMATODES

Didik Photo Collection

- Size 500-800 um,- Baktery simbion: Xenorhabdus spp.- dariSteinernema spp.Photorhabdus sp.- dari Heterorhabditis spp.- Efektif on all pest like:-Pest (Lepidoptera,-Coleoptera, Diptera, dll)- Hoticulture, Rice, Fruit, Industrial Crop, Crop Plantation, - Isolated from any kind of soil


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LIFE CYCLE OF LIFE CYCLE OF ENTOMO-ENTOMO-PATHOGENIC PATHOGENIC NEMATODESNEMATODES


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INFECTION MECHANISM OF ENTOMOPATHOGENIC INFECTION MECHANISM OF ENTOMOPATHOGENIC NEMATODES IN INSECTNEMATODES IN INSECT

Didik Sulistyanto Photo Collection


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INSECT MORTALITY WITHIN 24 HOURSINSECT MORTALITY WITHIN 24 HOURS



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Nematodes penetrated in anus of P. xylostella



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PATHOGENICITY OF EPNs SYMBIOTIC BACTERIA

Bakteria

Nematodes

Didik Photo Collection


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MECHANISM OF PENETRATION OF EPNs MECHANISM OF PENETRATION OF EPNs IN INSECT PESTSIN INSECT PESTS



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IDENTIFICATION TECHNIQUE OFIDENTIFICATION TECHNIQUE OF ENTOMOPATHOGENIC NEMATODES ENTOMOPATHOGENIC NEMATODES

Identification technique with:

(a) Infektif Juvenile (IJs),

(b) Adult Male,

(c) Molekuler biology,


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IDENTIFICATION ON INFECTIVE JUVENILEIDENTIFICATION ON INFECTIVE JUVENILE


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HEAD OF HEAD OF HETERORHABDITIS HETERORHABDITIS SPP.SPP.


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IDENTIFICATION OF HETERORHABDITIS SPP. ADULT MALE


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IDENTIFICATION OF STEINERNEMA SPP. ADULT MALE


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HETERORHABDITISHETERORHABDITIS SPP. ADULT MALE SPP. ADULT MALE


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HETERORHABDITISHETERORHABDITIS SPP. ADULT MALE SPP. ADULT MALE


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STEINERNEMASTEINERNEMA SPP. ADULT MALE SPP. ADULT MALE


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STEINERNEMASTEINERNEMA SPP. ADULT MALE SPP. ADULT MALE


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ENTOMOPATHOGENIC NEMATODES SPECIESENTOMOPATHOGENIC NEMATODES SPECIES

ENTOMOPATOGENIC 2 GENUS:ENTOMOPATOGENIC 2 GENUS:

(a) (a) HeterorhabditisHeterorhabditis sp.- sp.- PhotorhabduPhotorhabdus sp.s sp.

(b) (b) SteinernemaSteinernema sp.- sp.- XenorhabdusXenorhabdus sp. sp.

HETERORHABDITISHETERORHABDITIS SP.: SP.:

- H. indicaH. indica

- H. bacteriophoraH. bacteriophora

- H. popilliaeH. popilliae

- Heterorabhditis spp. Heterorabhditis spp.


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STEINERNEMASTEINERNEMA SP.: SP.:

- S. carpocapsaeS. carpocapsae

- S. affinisS. affinis

- S. glaseriS. glaseri

- S. bicornutumS. bicornutum

- S. feltiaeS. feltiae

- S. japanicumS. japanicum

- Steinernema spp. Steinernema spp.


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SYMBIOTIC OF BACTERIA SYMBIOTIC OF BACTERIA ENTOMOPATHOGENIC NEMATODESENTOMOPATHOGENIC NEMATODES

• Symbiotic bacteria of entomopathogenic Symbiotic bacteria of entomopathogenic nematodes live in Vesikel,nematodes live in Vesikel,

•Symbiotic bacteria have 2 phase: primer and Symbiotic bacteria have 2 phase: primer and secondary phase,secondary phase,

• Very difficult to separate with Host,Very difficult to separate with Host,

• Very sensitive with Ultra VioletVery sensitive with Ultra Violet

• 200 – 2000 cell of symbiotic bacteria per EPN,200 – 2000 cell of symbiotic bacteria per EPN,


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SYMBIOTIC BACTERIA OF SYMBIOTIC BACTERIA OF ENTOMOPATOGENIC NEMATODESENTOMOPATOGENIC NEMATODES


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SYMBIOTIC BACTERIA OF SYMBIOTIC BACTERIA OF Xenorhabdus nematophilusXenorhabdus nematophilus



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SYMBIOTIC SYMBIOTIC BACTERIA 0F - BACTERIA 0F - Photorhabdus Photorhabdus luminenscensluminenscens



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LIVE Steinernema sp. Heterorhabditis sp.



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ORBITAL SHAKER FOR PRODUCTIONORBITAL SHAKER FOR PRODUCTION


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Strain Collection

Mass Production

In Vivo

INSECT

In Vitro

Small Scale

Medium Scale Large Scale

Media Agar

Media Bedding

Bio-reaktor Liquid Culture

MASS PRODUCTION SCHEMEMASS PRODUCTION SCHEME


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MASS PRODUCTION TECHNIQUE OF MASS PRODUCTION TECHNIQUE OF ENTOMOPATHOGENIC NEMATODESENTOMOPATHOGENIC NEMATODES

•(1) (1) IN VIVO METHODSIN VIVO METHODS (INSECT CULTURE): (INSECT CULTURE):

- Tenebrio mollitorTenebrio mollitor,,

-Galleria mellonellaGalleria mellonella,,

- with WHITE TRAP method,with WHITE TRAP method,

- harvest after 7-10 days,harvest after 7-10 days,

- on petridis with paper and water,on petridis with paper and water,

- keep in room temperature and dark avoid the keep in room temperature and dark avoid the Ultra Violet,Ultra Violet,


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In-Vivo



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• (2) (2) IN VITROIN VITRO METHODS METHODS- use solid or liquid culture,use solid or liquid culture,

- harvest after 7-14 days and filter by 30 um mesh,harvest after 7-14 days and filter by 30 um mesh,

- keep in room temperature and dark avoid the Ultra keep in room temperature and dark avoid the Ultra Violet.Violet.

MASS PRODUCTION TECHNIQUE OF MASS PRODUCTION TECHNIQUE OF ENTOMOPATHOGENIC NEMATODESENTOMOPATHOGENIC NEMATODES


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IN VIVO IN VITRO

MASS PRODUCTION OF ENTOMPATHOGENIC NEMATODES IN VIVO

AND IN VITRO


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PRODUCTIONPRODUCTION IN VITRO IN VITRO (Sponge)(Sponge)



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MASS PRODUCTION IN FLASK



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MASS MASS PRODUCTION PRODUCTION TECHNIQUE TECHNIQUE OF EPNsOF EPNs

(a) (a) In vivoIn vivo(b) (b) In vitroIn vitro in media in media agaragar© © In vitroIn vitro in solid in solid media media (d) Harvest technique(d) Harvest technique



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MASS PRODUCTION EPNS IN BIOREACTOR IN VITROMASS PRODUCTION EPNS IN BIOREACTOR IN VITRO

IN VITRO BIOREAKTORDidik Sulistyanto Photo Collection


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Bio-Fermentor For Mass Production of Entomopathogenic Nematodes in Liquid media volume 50.000 - 100.000 liter



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SIMPLE HARVEST SIMPLE HARVEST TECHNIQUE OF TECHNIQUE OF ENTOMOPATHOGENIENTOMOPATHOGENIC NEMATODESC NEMATODES



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FORMULATION TECHNIQUE OF ENTOMOPATHOGENIC NEMATODES

(a) Sponge,(b) Mineral soil,(c) Mountain soil,(d) Dust,(e) Extract mineral,



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MASS PRODUCTION OF ENTOMOPATHOGENIC MASS PRODUCTION OF ENTOMOPATHOGENIC NEMATODES FOR FARMERSNEMATODES FOR FARMERS

• IN VIVO METHOD:- Serangga di kumpulkan dan diletakkan dalam

wadah yang berisi kertas serap air/tanah pasir,

- Larva diinokulasi dengan NEP dan diusahakan

jangan menggenang (kondisi lembab),

- Setelah serangga mati (warna coklat/merah),

disusun di wadah terbalik yang berada dalam

wadah besar yang dilapisi kertas & berisi air,

- Tutup dan biarkan selama 1- 2 minggu dan setelah itu

dipanen untuk diaplikasikan,


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ENTOMOPATHOGENIC NEMATODES PRODUCT ENTOMOPATHOGENIC NEMATODES PRODUCT AS BIOLOGICAL CONTROL OF PESTIN THE AS BIOLOGICAL CONTROL OF PESTIN THE

WORLDWORLD


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PRODUCT OF PRODUCT OF ENTOMOPATHOGENIC ENTOMOPATHOGENIC NEMATODES LOCAL NEMATODES LOCAL ISOLATES FROM ISOLATES FROM INDONESIAINDONESIA


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ENTOMOPATHOGENIC NEMATODES PRODUCT ENTOMOPATHOGENIC NEMATODES PRODUCT FOR CONTROL HORTICULTURE INSECTS PESTFOR CONTROL HORTICULTURE INSECTS PEST


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ENTOMOPATHOGENIC NEMATODES PRODUCT ENTOMOPATHOGENIC NEMATODES PRODUCT FOR CONTROL WHITE GRUBS INSECTS PESTFOR CONTROL WHITE GRUBS INSECTS PEST



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ENTOMOPATHOGENIC NEMATODES PRODUCT ENTOMOPATHOGENIC NEMATODES PRODUCT FOR CONTROL COFFEE AND CACAO INSECTS FOR CONTROL COFFEE AND CACAO INSECTS

PESTPEST


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ENTOMPPATHOGENIC NEMATODES PRODUCT FOR ENTOMPPATHOGENIC NEMATODES PRODUCT FOR CONTROL FLIES INSECTS PEST ON CAMPIGNONCONTROL FLIES INSECTS PEST ON CAMPIGNON


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PEMANFAATAN NEP SEBAGAI AGENSIA PEMANFAATAN NEP SEBAGAI AGENSIA HAYATI HAMAHAYATI HAMA

• Effektif mengendalikan hama dari ordo Effektif mengendalikan hama dari ordo Lepidoptera (kupu-kupu), Coleoptera (kumbang), Lepidoptera (kupu-kupu), Coleoptera (kumbang), Diptera (lalat), dan serangga hama lainnya,Diptera (lalat), dan serangga hama lainnya,

• Aman terhadap musuh alami (parasitoid, Aman terhadap musuh alami (parasitoid, predator dan musuh alami lainnya)predator dan musuh alami lainnya)

• Kompatibel diaplikasikan dengan pengendalian Kompatibel diaplikasikan dengan pengendalian kimiawi dan hayati lainnya,kimiawi dan hayati lainnya,

• Aman dimanfaatkan untuk tanaman pangan, Aman dimanfaatkan untuk tanaman pangan, sayuran, perkebunan dan jenis tanaman lainnya,sayuran, perkebunan dan jenis tanaman lainnya,

• Diaplikasikan pada sore hari untuk menghindari Diaplikasikan pada sore hari untuk menghindari sinar matahari (UV).sinar matahari (UV).


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Jenis-Jenis Hama Pada Tanaman Pertanian yang Dapat Dikendalikan dengan Nematoda Entomopatogen

(Steinernema sp. & Heterorhabditis sp.)


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FIELD RESEARCH IN BROMO MOUNTAIN



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Cabbage destructed by Plutella xylostella and Crocidolomia binotalis

Cabbage Normal

CABBAGE PROTECTED BY ENTOMOPATHOGENIC NEMATODES



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Plutella xylostella and Crocidolomia sp. Insect Pests Mortality 80 - 90% in the field control with EPNs



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(a) TERINFEKSI (b) SEHAT

a b

ab

P. xylostella

INFECTION OF EPNs ON SEVERALINSECT PESTS



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CABBAGE WITH FULL PESTICIDE


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Khob Khun KhrabEmail:

[email protected]

www.didiksulis.blogspot.com

HP : 08124982150

PROSPECT OF THE ENTOMOPATHOGENIC NEMATODES AS BIOLOGICAL CONTROL FOR THE FUTURE IN INDONESIA AND...

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