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Project Nr 16 Tissue factor, tissue factor pathway inhibitor, and thrombin-antithrombin complexes in...
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Project Nr 16
Tissue factor, tissue factor pathway inhibitor,and thrombin-antithrombin complexes in patients
with abdominal aortic aneurysm undergoing endovascular and open repair.
Kotschy M.1, Witkiewicz W.1,2, Grendziak R.2, Zapotoczny N.2, Dubis J.1, Żuk N.1, Pfanhauser M.2, Kotschy D.3
1 Regional Specialized Hospital in Wrocław, Research & Development Centre Departments: 2 of Vascular Surgery, 3 of Angiology
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The aim of the study was to determinethe concentrations of TF, TFPI, VEGF-A
and TAT complexes in blood plasma of patientswith abdominal aortic aneurysm undergoing
endovascular aortic repair (EVAR)
and open aortic repair (OAR)
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• Background
Source: http://www.sirweb.org/patients/abdominal-aortic-aneurysms/
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EndoVascular Aortic Repair
Source: http://leedsvascularinstitute.org/AAA_Education_EVAR.php
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OpenAorticRepair
Source: http://www.surgeryencyclopedia.com/A-Ce/Aortic-Aneurysm-Repair.html#b
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The presence of tissue factor (TF), tissue factor pathway inhibitor (TFPI), vascular endothelial growth factor-A (VAEGF-A) in patients with
damaged aortic wall, with thrombin-antithrombin complexes (TAT) indicating thrombin-generation and appearance of intraluminal thrombin in aortic
aneurysm suggests that haemostatic processes can enhance proteolysis of aortic wall.
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Haemostatic factors:
TF, TFPI TAT complex
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Haemostasis VEGF
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VEGF - A as angiogenic factor
Source: http://www.biooncology.com
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Materials and methodsThe studies enrolled 42 patients (38 males and females)in mean age 71 (range 59-86) years in whom presurgery ultrasound and computer tomography revealed presence
of abdominal aortic aneurysm (AAA). Blood was drawn 3 times: before, directly after surgery and 24 hours after EVAR
and OAR. The concentrations of TF, TFPI, VEGF-Aand TAT complexes was measured in plasma using
enzyme immuno-assay with commercial tests.
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Kind of repair EVAR n (n = 26 %)
OARn (n=16%)
Gengermale/ female
24/2 92,3/7,7 14/2 87,5 / 12,5
Age (years)mean-range
68 (59-83) 75 (64-86)
Coronary heart diseases
11 34 8 50
Previous Myocardial Infarction
9 29 4 25
Cerebrovascular disease
6 19 2 12,5
Preipheral vascular diseases
2 6 0 0
Hypertension 9 29 6 37,5
Pulmonary diseases 7 22 1 6,25
Renal, prostate diseases
8 25 4 25
Diabetes mellutis 3 9,3 1 6,25
Smoking 20 62,5 12 75
Aneurysm diameter cm range
5,43,5-7,7
8,14,5-10,0
Tab. 1 Some demographic data
of examined patients with AAA
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Fig.1
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Examined parameters
Controlsn = 50M; SD (range)Me, Q1, Q3
Patients with AAAn = 42M; SD (range)Me, Q1, Q3
p-value
TFpg/ml
133+/- 79(18-290)
230 +/- 139(81-912)
0,03
TFPIng/ml
84+/- 19(52-124)
90+/-23(51-174)
NS
VEGF-Apg/ml
24+/- 14(8-44)
34+/-23(14-144)
NS
TAT complexng/ml
2,2+/-3,5(0,5 – 4,9)Me; Q1, Q31,4; 0,7; 2,2
7,6+/-8,2(1,1 - 33)Me; Q1, Q35,24;2,7;8,8
0,0003
Tab. 2 Haemostatic factors in plasma of patients with AAA and in controls
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Examined parameters
EVAR n=26
2Preoperative
M; SD
3End of surgery
M; SD
424 h postoperative
M; SD
p-value
TFpg/ml
229+/- 162(98-912)
131+/-89(60-443)
203+/-112(70-483)
2/3 0,00013/4 0,01
TFPIng/ml
101+/-18(66-134)
114+/-29(65-169)
125+/-30(77-194)
2/30,006
VEGFpg/ml
25+/-9(13-42)
21+/-12(6-43)
33+/-16(14-62
NS
TAT complexesng/ml
9,9+/-9,8
Me, Q1, Q37,8 2,9 12,9
37,9+/-21,6
Me, Q1, Q331,6 25,6 52,0
18,9+/-31,6
Me, Q1, Q39,9 5,9 13,6
2/3 0,00033/4 0,034
Tab. 3 Haemostatic factors during endovascular aortic repair
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1 – control 2 – preoperative3 – immediatelly postoperative 4 – postoperative (24 h)
EVAR - endovascular aortic repair
Concentration of TF in Medians during EVAR
Fig. 2
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Tab. 4 Haemostatic factors during open aortic repair (OAR)
Examined parameters
OAR n=16
2Preoperative
M; SD
3End of surgery
M; SD
424 h postoperative
M; SD
p-value
TFpg/ml
223+/- 96(75-397)
161+/-88(53-390)
218+/-75(150-389)
2/3, 3/4 <0,02
TFPIng/ml
88+/-24(51-137)
97+/-38(63-174)
87+/-23(62-122)
SN
VEGFpg/ml
38+/-19(13-61)
36+/-30(7-118)
50+/-21(8-84)
SN
TAT complexesng/ml
4,7+/-3,7(0,7 – 14,1)Me, Q1, Q34,3 1,6 14,1
34,5+/-27,2(6,5 – 90,2)Me, Q1, Q324,9 11,2 50,4
51,3+/-42,2(6,4 – 104,8)Me, Q1, Q362 8,2 70,5
2/3 0,00533/4 0,000005
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TAT complexes in Medians and Quartiles
1 – control 2 – preoperative3 – immediatelly postoperative 4 – postoperative (24 h)
Fig. 3
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TAT complexes during OAR
The relation of its preoperative concentration
1 – control 2 – preoperative3 – immediatelly postoperative 4 – postoperative (24 h)
Fig. 4
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Conclusions:
1.Significantly higher [TF] & [TAT] in AAA patients
than in controls.
2.EVAR & OAR activates thrombin-generation in AAA patients
3.The degree of clotting activation after aortic aneurysm repair
depends on preoperative clotting status of patients with AAA.
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References:
1.Yamazumi K., Ojro M., Okumura H., Aikon T. ‘An activated state of blood coagulation and fibrynolysis in patients withb abdominal aortic aneurysm.’ Am. J. Surg. 1998; 775:297-301.2.Hobbs S.D., Haggart P., Fegan Ch., Bradburry A.W., Adam D.J. ‘The role of tissue factor in patients undergoing open repair of raptured and nonraptured abdominal aortic aneurysm.’ J. Vasc. Surg. 2007;46:682-686.