Programacion de Pruebas Manuales Elitech

SEPPIM S.A.S.

ZONE INDUSTRIELLE 61500 SEES - FRANCE

: 02 33 81 21 00 : 02 33 28 77 51

DIAGNOSTIC REAGENTS

MANUAL METHODS

Version : MMAN-09 – April 2010

SEPPIM S.A.S.

ZONE INDUSTRIELLE 61500 SEES - FRANCE

: 02 33 81 21 00 : 02 33 28 77 51

REAGENTS

A Version

ACID PHOSPHATASE .................................MM-PACI-1

ALBUMIN .................................................. MM-ALBU-1

ALP (DEA) SL .............................................. MM-PASL-1 ALP (DEA) ................................................... MM-PALC-1

ALT/GPT 4+1 SL .................................... MM-ALSL4/1-1 ALT/GPT ..................................................... MM-ALAT-2

AMYLASE SL ............................................. MM-AMSL-3

AST/GOT 4+1 SL .................................... MM-ASSL4/1-1 AST/GOT ..................................................... MM-ASAT-2

B

BILIRUBIN TOTAL & DIRECT 4+1 .......... MM-BITD-2

C

CALCIUM ARSENAZO ............................. MM-CALA-1 CALCIUM OCPC ........................................ MM-CALO-1

Update CHLORIDE ................................................. MM-CHLO-2

CHOLESTEROL SL ..................................... MM-CHSL-2 Update CHOLESTEROL ......................................... MM-CHOL-3 CHOLESTEROL HDL SL 2G .................... MM-HDLL-1 CHOLESTEROL LDL SL 2G ...................... MM-LDLL-1 HDL CHOLESTEROL ................................ MM-HDLC-2

CHOLINESTERASE .................................... MM-CHES-2

CK MB SL ................................................... MM-CMSL-2 CK MB ........................................................ MM-CKMB-2

CK NAC SL .................................................. MM-CKSL-2 CK NAC ....................................................... MM-CKNA-2

COPPER........................................................ MM-CUIV-1

CREATININE JAFFE .................................. MM-CRCO-1 CREATININE PAP SL ................................. MM-CRSL-3

G Version GAMMA GT SL ........................................... MM-GASL-1 GAMMA GT ............................................... MM-GAGT-1

GLUCOSE HK SL ........................................ MM-GHSL-1 GLUCOSE PAP SL ...................................... MM-GPSL-2 GLUCOSE PAP ............................................ MM-GLUP-1

I IRON CHROMAZUROL ............................. MM-FECA-1 IRON FERROZINE® ................................... MM-FEFR-2

L

LACTATE .................................................... MM-LACT-2

New LDH-L SL ......................................................MM-LLSL-1 LDH-P .......................................................... MM-LDHP-2

M

MAGNESIUM CALMAGITE ................... MM-MAGN-1

MICROPROTEIN ........................................ MM-PRTP-2

P

PHOSPHORUS ............................................ MM-PHOS-1

T TOTAL PROTEIN PLUS ............................. MM-PROB-1

TRIGLYCERIDES MONO SL NEW ...... MM-TGMLN-2 TRIGLYCERIDES ........................................ MM-TRIG-2

U

UREA UV SL ............................................... MM-URSL-2 UREA UV .................................................... MM-URUV-1

URIC ACID ................................................. MM-ACUR-2 URIC ACID MONO SL .............................. MM-AUML-2 Update URIC ACID SL ............................................ MM-AUSL-2

ELITECH ELITECH ELITECH

SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

ACID PHOSPHATASE

Ref. : PACI- 0030 18 x 3 mL

MANUAL METHOD

PROCEDURE Wavelength : 405 nm Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank.

Mix and after a 5 minute incubation measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION Total ACP : Activity (U/L) = ∆OD/min. x 853 NP ACP : Activity (U/L) = ∆OD/min. x 860 Prostatic fraction (U/L) = Total ACP (U/L) - NP ACP (U/L). LINEARITY If ∆OD/min. exceeds 0.070 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(07/2005) MM-PACI-1

Total acid phosphatase Non-prostatic acid phosphataseWorking Reagent 1 1 mL 1 mL Working Reagent 2 10 µL Sample 100 µL 100 µL



ALBUMIN

Ref.: ALBU - 0600 2 x 125 mL ALBU - 0700 4 x 250 mL

MANUAL METHOD


Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 15 minutes. CALCULATION OD Sample x n OD Standard n = standard concentration

NOTE: For more details, see the Instructions for use

(07/2005) MM-ALBU-1

BLANK STANDARD SAMPLE Reagent 1 mL 1 mL 1 mL Distilled Water 10 µL Standard 10 µL Sample 10 µL



ALP (DEA) SL

Réf. : PASL- 0400 2 x 62.5 mL PASL- 0420 4 x 62.5 mL PASL- 0500 5 x 125 mL

MANUAL METHOD

PROCEDURE Wavelength : 405 nm (410) Temperature : 37°C, 30°C, 25°C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure

Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. • Two-reagent procedure

Mix, wait 1 minute and add:

Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION One reagent procedure Two reagents procedure At 405 nm: Activity (U/L) = ∆OD/min. x 2 750 3424 At 410 nm: Activity (U/L) = ∆OD/min. x 2 910 3623 LINEARITY If ∆OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(07/2005) MM-PASL-1

Working reagent : 1 mL Sample : 20 µL

R1 : 1 mL Sample : 20 µL

R2 : 250 µL



ALP (DEA)

Réf. : PALC- 0030 20 x 3 mL PALC- 0200 12 x 20 mL

MANUAL METHOD

PROCEDURE Wavelength : 405 nm (410) Temperature : 25°C, 30°C, 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION At 405 nm: Activity (U/L) = ∆OD/min. x 2 750 At 410 nm: Activity (U/L) = ∆OD/min. x 2 910 LINEARITY If ∆OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(07/2005) MM-PALC-1




ALT/GPT 4+1 SL

Ref.: ALSL – 0410 2 x 62.5 mL ALSL – 0430 4 x 62.5 mL ALSL – 0510 5 x 125 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm Temperature : 37°C Cuvette : 1 cm light path Read against distilled water. • One-reagent procedure



Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION For the two procedures, at 340 nm: Activity (U/L) = ∆OD/min. x 1 746 LINEARITY If ∆OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(07/2005) MM-ALSL4/1-1



R2 : 250 µL



ALT/GPT

Ref.: ALAT - 0200 12 x 20 mL ALAT - 0400 9 x 50 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm (334 – 365) Temperature : 30°C, 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION At 340 nm: Activity (U/L) = ∆OD/min. x 1 746 At 334 nm: Activity (U/L) = ∆OD/min. x 1 780 At 365 nm: Activity (U/L) = ∆OD/min. x 3235 LINEARITY If ∆OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(03/2008) MM-ALAT-2




AMYLASE SL

Réf. : AMSL - 0390 1 x 50 mL AMSL - 0400 6 x 50 mL

MANUAL METHOD

PROCEDURE Wavelength : 405 nm Temperature : 30°C, 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION 37 °C 30 °C At 405 nm: Activity (U/L) = ∆OD/min. x 4640 2812 Take dilution factor into account for the calculation of the activity in urine. LINEARITY Up to 900 U/L If ∆OD/min. exceeds 0.190 at 405 nm, repeat test using sample diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(11/2006) MM-AMSL-3

37 °C 30 °C Reagent 1 mL 1 mL Sample 15 µL 25 µL



AST/GOT 4+1 SL

Réf. : ASSL- 0410 2 x 62.5 mL ASSL- 0430 4 x 62.5 mL ASSL- 0510 5 x 125 mL

MANUAL METHOD




Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION For the two procedures, at 340 nm: Activity (U/L) = ∆OD/min. x 1 746 LINEARITY If ∆OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(07/2005) MM-ASSL4/1-1



R2 : 250 µL



AST/GOT

Ref.: ASAT - 0200 12 x 20 mL ASAT - 0400 9 x 50 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm (334 – 365) Temperature : 30°C, 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION At 340 nm: Activity (U/L) = ∆OD/min. x 1 746 At 334 nm: Activity (U/L) = ∆OD/min. x 1 780 At 365 nm: Activity (U/L) = ∆OD/min. x 3235 LINEARITY If ∆OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(07/2008) MM-ASAT-2




BILIRUBIN

TOTAL & DIRECT 4+1

Ref.: BITD – 0600 Total 125 mL

Direct 125 mL

BITO - 0600 Total 2 x 125 mL

BIDI - 0600 Direct 2 x 125 mL

MANUAL METHOD

PROCEDURE for TOTAL BILIRUBIN

Wavelength : 550 nm Temperature : 37°C Cuvette : 1 cm light path

Read against distilled water.

CALIBRATION TEST

R1 BILIRUBIN TOTAL 4+1 800 µL 800 µL

Calibrator 100 µL

Sample 100 µL

Mix and read the optical density (OD) after 5 minutes of incubation at 37°C

OD1 OD2

Then add:

R2 BILIRUBIN TOTAL & DIRECT 4+1 200 µL 200 µL


OD3 OD4

PROCEDURE for DIRECT BILIRUBIN

Wavelength : 550 nm Temperature : 37°C Cuvette : 1 cm light path

Read against distilled water.

CALIBRATION TEST

R1 BILIRUBIN DIRECT 4+1 800 µL 800 µL

Calibrator 100 µL

Sample 100 µL


OD1 OD2

Then add:

R2 BILIRUBIN TOTAL & DIRECT 4+1 200 µL 200 µL


OD3 OD4

CALCULATION FOR BOTH PROCEDURES (Total and Direct Bilirubin)

With Factor: (OD4 - OD2) x F

mg/dL: F = 14.0

mg/L: F = 140

µmol/L: F = 240

With calibrator: OD4 - OD2 x n

OD3 - OD1

n = Calibrator concentration

NOTE: For more details, see the Instructions for use (08/2009)

MM-BITD-2



CALCIUM ARSENAZO

Ref.: CALA - 0600 2 x 125 mL

MANUAL METHOD

PROCEDURE Wavelength : 650 nm (600) Temperature : 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and read the optical density (OD) after a 1 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard n = standard concentration Take dilution factor into account for the calculation of the concentration in urine.


(07/2005) MM-CALA-1




CALCIUM OCPC

Ref.: CALO - 0600 2 x 125 mL

MANUAL METHOD

PROCEDURE Wavelength : 570 nm Temperature : 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard n = standard concentration Take dilution factor into account for the calculation of the concentration in urine.


(07/2005) MM-CALO-1

BLANK STANDARD SAMPLE Working Reagent 1 mL 1 mL 1 mL Distilled Water 10 µL Standard 10 µL Sample 10 µL


(09/2009)

MM-CHLO-2


CHLORIDE

Réf. : CHLO – 0600 2 x 125 mL

250 mL

MANUAL METHOD

PROCEDURE

Wavelength : 480 nm

Temperature : 25°C, 30°C, 37°C

Cuvette : 1 cm light path

Read against reagent blank.

Mix and read the optical density (OD) after a 5 minute incubation.

The final colour is stable for at least 1 hour.

Information for urine sample: The urine sample must be diluted ½ with distilled water before analysis.

CALCULATION

Serum/Plasma: Urine:

n StandardOD

SampleOD 2n

StandardOD

SampleOD

n= Standard concentration


BLANK STANDARD SAMPLE

Reagent 1 mL 1 mL 1 mL

Distilled Water 10 µL

Standard 10 µL

Sample 10 µL


SEPPIM S.A.S. ZONE INDUSTRIELLE - 61500 SEES - FRANCE

CHOLESTEROL SL

Réf. : CHSL- 0490 1 x 100 mL CHSL- 0500 6 x 100 mL CHSL- 0700 4 x 250 mL CHSL- 0507 6 x 100 mL* CHSL- 0707 4 x 250 mL*

MANUAL METHOD

PROCEDURE Wavelength : 500 nm (492-550) Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank.

Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 1 hour. CALCULATION OD Sample x n OD Standard

n= Standard concentration NOTE: For more details, see the Instructions for use

(07/2007) * these kits contain a standard MM-CHSL-2




CHOLESTEROL

Ref.: CHOL - 0220 12 x 20 mL CHOL - 0420 9 x 50 mL

MANUAL METHOD

PROCEDURE Wavelength : 500 nm (492 – 550) Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank.




(03/2010) MM-CHOL-3




CHOLESTEROL HDL SL 2G

Ref.: HDLL – 0380 1 x 80 mL HDLL – 0390 3 x 80 mL

MANUAL METHOD

PROCEDURE Wavelength : 600 nm Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank. BLANK STANDARD SAMPLE R1 900 µL 900 µL 900 µL Distilled water 9 µL Standard 9 µL Sample 9 µL Mix and read absorbance (A1) after 5 minutes of incubation at 37°C. Then add: R2 300 µL 300 µL 300 µL Mix and read absorbance (A2) after 5 minutes of incubation at 37°C CALCULATION

nCalibratorFdilAA

SampleFdilAA×

×−×−

)()(

12

12 n = Calibrator concentration

Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume) NOTE: For more details, see the Instructions for use

(04/2009) MM-HDLL-1



CHOLESTEROL LDL SL 2G

Ref.: LDLL – 0380 1 x 80 mL

MANUAL METHOD

PROCEDURE Wavelength : 550 nm Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank. BLANK STANDARD SAMPLE R1 900 µL 900 µL 900 µL Distilled water 9 µL Standard 9 µL Sample 9 µL Mix and read absorbance (A1) after 5 minutes of incubation at 37°C. Then add: R2 300 µL 300 µL 300 µL Mix and read absorbance (A2) after 5 minutes of incubation at 37°C CALCULATION

nCalibratorFdilAA

SampleFdilAA×

×−×−

)()(

12

12 n = Calibrator concentration

Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume) NOTE: For more details, see the Instructions for use

(04/2009) MM-LDLL-1



HDL

CHOLESTEROL

Ref.: HDLC - 0060 3 x 10 mL x 100 mL

MANUAL METHOD

PROCEDURE

1) Sample preparation: Add to 500 µL of sample, 50 µL of precipitating reagent. Mix, wait for 10 minutes and centrifuge at 5000 rpm

for 15 minutes. The supernatant is collected for HDL determination.

2) HDL determination:

Wavelength : 500 nm (492-550)

Temperature : 37°C




The final colour is stable for at least 1 hour.

CALCULATION

OD Sample x n x 1.1

OD Standard

n = Standard concentration

1.1 = Sample dilution factor


(09/2009)

MM-HDLC-2


Working Reagent 1 mL 1 mL 1 mL


Standard 50 mg/dL 50 µL

Supernatant 50 µL



CHOLINESTERASE

Ref.: CHES - 0053 16 x 3 mL

MANUAL METHOD

PROCEDURE Wavelength : 405 nm (400 –440) Temperature : 25°C, 30°C, 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and measure the change of optical density every 30 seconds (∆OD/30 s.) during 90 seconds. CALCULATION Activity (U/L) = ∆OD/30 s. x 23 460 LINEARITY If ∆OD/30 sec. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/5 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(03/2008) MM-CHES-2

Working reagent 1 : 3 mL Sample : 20 µL Working reagent 2 : 100 µL



CK MB SL

Réf. : CMSL- 0410 2 x 62.5 mL CMSL- 0430 4 x 62.5 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm Temperature : 30°C, 37°C Cuvette : 1 cm light path Read against distilled water. • One-reagent procedure

Mix and after a 3 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes.

• Two-reagent procedure

Mix and wait 30 seconds

Mix and after a 5 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION For the two procedures, at 340 nm: Activity (U/L) = ∆OD/min. x 8254 LINEARITY Inhibition : up to 2 000 U/L of CK-MM at 37° C. If ∆OD/min. exceeds 0.075 dilute the sample (one part with nine parts saline solution), repeat the test and multiply the result by 10. Linear up at least 600 U/L. NOTE: For more details, see the Instructions for use

(11/2008) : Modification from the previous version MM-CMSL-2


R1 : 1 mL R2 : 250 µL

Sample : 50 µL



CK MB

Réf. : CKMB- 0030 20 x 3 mL

MANUAL METHOD


Mix and after a 5 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION

a) Total CK activity : Determine total CK activity in serum using the CK-NAC reagent. b) CK-B activity : CK-B (U/L) = ∆OD/min x 3 333 c) CK-MB activity : CK-MB (U/L) = CK-B (U/L) x 2 d) Percentage of CK-MB activity in sample : % CK-MB = CK-MB x 100 Total-CK

LINEARITY Inhibition : up to 2000 U/L of CK-MM at 37° C. If the assay of the total CK exceeds 1200 U/L at 37°C, dilute the sample appropriately with physiological saline before assay of CK-MB. Multiply the result by the dilution factor to obtain the correct value of the isoenzyme. NOTE: For more details, see the Instructions for use

(05/2006) MM-CKMB-2




CK NAC SL

Réf. : CKSL- 0410 2 x 62.5 mL CKSL- 0430 4 x 62.5 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm Temperature : 30°C, 37°C Cuvette : 1 cm light path Read against distilled water. One-reagent procedure

Mix and after a 2 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes.

• Two-reagent procedure

Mix and wait 30 seconds

Mix and after a 2 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION For the two procedures, at 340 nm: Activity (U/L) = ∆OD/min. x 4127 LINEARITY If ∆OD/min. exceeds 0.250 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(11/2008) : Modification from the previous version MM-CKSL-2


R1 : 1 mL R2 : 250 µL

Sample : 50 µL



CK NAC

Réf. : CKNA- 0030 20 x 3 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm (334-365) Temperature : 25°C, 30°C, 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and after a 2 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION At 340 nm : Activity (U/L) = ∆OD/min. x 8 095 At 334 nm : Activity (U/L) = ∆OD/min. x 8 252 At 365 nm : Activity (U/L) = ∆OD/min. x 14 571

LINEARITY If ∆OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(03/2008) MM-CKNA-2




COPPER

Réf. : CUIV- 0050 5 x 10 mL

MANUAL METHOD

PROCEDURE Wavelength : 580 nm (570-590) Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank. One-reagent procedure

Mix and read the optical density (OD) after a 5 minute incubation. • Two-reagent procedure

Mix and read the optical density (OD1).

Mix and read the optical density (OD2) after a 5 minute incubation. The final colour is stable for at least 1 hour. CALCULATION • One-reagent procedure : OD Sample x n OD Standard

• Two-reagent procedure : OD2 - OD1 Sample x n OD2 - OD1 Standard

n = standard concentration NOTE: For more details, see the Instructions for use

(07/2005) MM-CUIV-1

BLANK STANDARD SAMPLE Working Reagent 2 1 mL 1 mL 1 mL Distilled Water 50 µL Standard 50 µL Sample 50 µL

BLANK STANDARD SAMPLE Working Reagent 1 1 mL 1 mL 1 mL Distilled Water 50 µL Standard 50 µL Sample 50 µL

Reagent 2 50 µL 50 µL 50 µL



CREATININE JAFFE

Ref.: CRCO - 0600 2 x 125 mL CRCO - 0700 4 x 250 mL

MANUAL METHOD

PROCEDURE Wavelength : 492 nm (480 –520) Temperature : 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and read the optical density (OD1) 10 seconds after the sample or standard addition. Exactly 2 minutes after the first reading, take second reading (OD2). CALCULATION ∆OD Sample x n ∆OD Standard Take dilution factor into account for calculation of concentration in urine. NOTE: For more details, see the Instructions for use

(07/2005) MM-CRCO-1




CREATININE PAP SL

Ref.: CRSL - 0630 2 x 133 mL

MANUAL METHOD


Mix, and wait 5 minutes at 37°C. Then, add:

Mix and read the change of optical density (∆OD) between 1 and 4 minutes.. CALCULATION ∆OD Sample x n ∆OD Standard n = standard concentration Take dilution factor into account for the calculation of the concentration in urine.


(03/2008) MM-CRSL-3

BLANK STANDARD SAMPLE R1 1.5 mL 1.5 mL 1.5 mL Distilled Water 35 µL Standard 35 µL Sample 35 µL

R2 0.5 mL 0.5 mL 0.5 mL



GAMMA GT SL

Ref.: GASL - 0400 2 x 62.5 mL GASL - 0420 4 x 62.5 mL GASL - 0500 5 x 125 mL

MANUAL METHOD

PROCEDURE Wavelength : 405 nm Temperature : 30°C, 37°C Cuvette : 1 cm light path Read against distilled water. • One-reagent procedure



Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION

One reagent procedure Two reagents procedure At 405 nm: Activity (U/L) = ∆OD/min. x 1 158 1421 LINEARITY If ∆OD/min. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(07/2005) MM-GASL-1



R2 : 250 µL



GAMMA GT

Ref.: GAGT – 0030 20 x 3 mL GAGT – 0200 12 x 20 mL

MANUAL METHOD


Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION At 405 nm: Activity (U/L) = ∆OD/min. x 1 111 LINEARITY If ∆OD/min. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L). Multiply result by 10. NOTE: For more details, see the Instructions for use

(07/2005) MM-GAGT-1




GLUCOSE HK SL

Ref.: GHSL - 0600 5 x 125 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm (334-365) Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank. • One-reagent procedure

Mix and read the optical density (OD) after a 5 minute incubation. • Two-reagents procedure


Mix and read the optical density (OD) after a 5 minute incubation. CALCULATION OD Sample x n OD Standard



(07/2005) MM-GHSL-1


BLANK STANDARD SAMPLE R1 1 mL 1 mL 1 mL Distilled Water 10 µL Standard 10 µL Sample 10 µL

R2 250 µL 250 µL 250 µL



GLUCOSE PAP SL

Ref.: GPSL - 0490 1 x 100 mL GPSL - 0500 6 x 100 mL GPSL - 0700 4 x 250 mL GPSL - 0507 6 x 100 mL* GPSL - 0707 4 x 250 mL*

MANUAL METHOD


Mix and measure the optical density (OD) after a 10 minute incubation. The reading can be done during 30 minutes. CALCULATION OD Sample x n OD Standard



(07/2007) * these kits contain a standard MM-GPSL-2




GLUCOSE PAP

Ref.: GLUP - 0700 4 x 250 mL GLUP - 0800 5 x 500 mL

MANUAL METHOD





(07/2005) MM-GLUP-1




IRON CHROMAZUROL

Ref.: FECA - 0600 2 x 125 mL

MANUAL METHOD





(07/2005) MM-FECA-1




IRON FERROZINE

Ref.: FEFR – 0600 2 x 125 mL

MANUAL METHOD

PROCEDURE Wavelength : 560 nm (550-570) Temperature : 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and read the optical density (OD).

Then add:

Mix and read the optical density (OD) within one hour.

CALCULATION OD4 - OD2 x n OD3 - OD1 n = standard concentration


(09/2009) MM-FEFR-2


- OD1 OD2

R2 50 µL 50 µL 50 µL

- OD3 OD4



LACTATE

Réf. : LACT- 0100 10 x 10 mL

MANUAL METHOD




(05/2006) MM-LACT-2




LDH-L SL

Ref.: LLSL - 0400 2 x 62.5 mL LLSL - 0420 4 x 62.5 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank. • Two-reagent procedure BLANK SAMPLE R1 1 mL 1 mL Distilled Water 35 µL Sample 35 µL Mix, wait 3 minute and add: R2 250 µL 250 µL Mix and after a 100 second incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION At 340 nm: Activity (U/L) = ∆OD/min. x 5828 NOTE: For more details, see the Instructions for use

(10/2007) MM-LLSL-1



LDH-P

Ref. : LDHP - 0030 20 x 3 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm (334-365) Temperature : 30°C, 37°C Cuvette : 1 cm light path Read against distilled water.

Mix and after a 1 minute incubation, measure the change of optical density per minute (∆OD/min.) during 3 minutes. CALCULATION At 340 nm: Activity (U/L) = ∆OD/min. x 8095 At 334 nm: Activity (U/L) = ∆OD/min. x 8252 At 365 nm: Activity (U/L) = ∆OD/min. x 15000 LINEARITY If ∆OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/5 or 1/10 with sodium chloride solution (9 g/L). Multiply result by 5 or 10. NOTE: For more details, see the Instructions for use

(03/2008) MM-LDHP-2




MAGNESIUM CALMAGITE

Ref.: MAGN - 0600 2 x 125 mL

MANUAL METHOD



n= Standard concentration Take dilution factor into account for the calculation of magnesium concentration in urine.


(07/2005) MM-MAGN-1




MICROPROTEIN

Ref.: PRTP - 0600 2 x 125 mL

MANUAL METHOD


Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for 10 minutes.

CALCULATION OD Sample x n OD Standard



(11/2008) : Modification from the previous version MM-PRTP-2




PHOSPHORUS

Ref.: PHOS - 0600 2 x 125 mL

MANUAL METHOD



n= Standard concentration Take dilution factor into account for the calculation of phosphorus concentration in urine.


(07/2005) MM-PHOS-1



(08/2009)

MM-PROB-1


TOTAL PROTEIN PLUS

Réf.: PROB - 0600 2 x 125 mL

PROB - 0700 4 x 250 mL

MANUAL METHOD

PROCEDURE

Wavelength : 546 nm

Temperature : 37°C



Mix and read the optical density (OD) after 10 minutes incubation.

The final colour is stable for 20 minutes.

CALCULATION

nStandardOD

SampleOD




Reagent 1 mL 1 mL 1 mL


Standard 10 µL

Sample 10 µL



TRIGLYCERIDES MONO SL NEW

Réf. : TGML-0425 6 x 50 mL TGML-0515 6 x 100 mL TGML-0700 4 x 250 mL TGML-0427 6 x 50 mL* TGML-0517 6 x 100 mL* TGML-0707 4 x 250 mL*

MANUAL METHOD




(07/2007) * these kits contain a standard MM-TGMLN-2




TRIGLYCERIDES

Ref.: TRIG - 0200 12 x 20 mL TRIG - 0400 9 x 50 mL

MANUAL METHOD

PROCEDURE Wavelength : 546 nm (520 – 570) Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank.

Mix and read the optical density (OD) after a 5 minute incubation. The final colour is stable for at least 30 minutes. CALCULATION OD Sample x n OD Standard



(07/2007) MM-TRIG-2




UREA UV SL

Réf. : URSL- 0400 2 x 62.5 mL URSL- 0420 4 x 62.5 mL URSL- 0500 5 x 125 mL URSL- 0407 2 x 62.5 mL* URSL- 0427 4 x 62.5 mL* URSL- 0507 5 x 125 mL*

MANUAL METHOD


Mix and read the variation of optical density (∆OD) between 30 seconds and 90 seconds. • Two-reagent procedure


Mix and read the variation of optical density (∆OD) between 30 seconds and 90 seconds. CALCULATION ∆OD Sample x n ∆OD Standard n = standard concentration NOTE: For more details, see the Instructions for use

(07/2007) * these kits contain a standard MM-URSL-2



R2 : 250 µL



UREA UV

Réf. : URUV-0400 9 x 50 mL URUV-0500 6 x 100 mL

MANUAL METHOD

PROCEDURE Wavelength : 340 nm Temperature : 37°C, 30°C Cuvette : 1 cm light path Read against reagent blank.

Mix and read the variation of optical density (∆OD) between 30 seconds and 90 seconds. CALCULATION ∆OD Sample x n ∆OD Standard


Take the dilution factor into account for the calculation of urea concentration in urine.


(07/2005) MM-URUV-1



SEPPIM S.A..S- ZONE INDUSTRIELLE - 61500 SEES - FRANCE

URIC ACID

Réf. : ACUR - 0200 12 x 20 mL

ACUR - 0400 9 x 50 mL

MANUAL METHOD

PROCEDURE

Wavelength : 510 nm (492-550)

Temperature : 37°C




The final colour is stable for at least 15 minutes.

CALCULATION

OD Sample x n

OD Standard


Take the dilution factor into account for the calculation of uric acid concentration in urine.


(09/2009)

MM-ACUR-2


Working Reagent 1 mL 1 mL 1 mL


Standard 25 µL

Sample 25 µL



URIC ACID MONO SL

Réf. : AUML - 0420 6 x 50 mL AUML - 0500 6 x 100 mL AUML - 0700 4 x 250 mL AUML - 0427 6 x 50 mL* AUML - 0507 6 x 100 mL* AUML - 0707 4 x 250 mL*

MANUAL METHOD




Take the dilution factor into account for the calculation of uric acid concentration in urine.


(07/2007) * these kits contain a standard MM-AUML-2




URIC ACID SL

Réf. : AUSL – 0400 2 x 62.5 mL AUSL – 0600 5 x 125 mL

MANUAL METHOD

PROCEDURE Wavelength : 555 nm Temperature : 37°C Cuvette : 1 cm light path Read against reagent blank. • One-reagent procedure

Mix and read the optical density (OD) after a 5 minute incubation. • Two-reagent procedure



n= Standard concentration Take the dilution factor into account for the calculation of uric acid concentration in urine.


(03/2010) MM-AUSL-2


BLANK STANDARD SAMPLE R1 1 mL 1 mL 1 mL Distilled Water 25 µL Standard 25 µL Sample 25 µL

R2 250 µL 250 µL 250 µL

Programacion de Pruebas Manuales Elitech

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