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PROFORMA – I

PROFORMA FOR SUBMISSION OF PROJECT PROPOSALS ON RESEARCH AND DEVELOPMENT, PROGRAMME SUPPORT

(To be filled by the applicant)

PART I: GENERAL INFORMATION

1. Name of the Institute/ Project Proposal : Dr. N.G.P. Arts and science college , Coimbatore

2. State : Tamilnadu

3. Status of the Institute : College affiliated to Bharathiar University, Coimbatore

4. Name and designation of the Executive Authority of the Institute/University forwarding the application : Dr. P.R. Muthuswamy, Principal Dr. N.G.P. Arts and Science College, Coimbatore

5. Project Title : Screening, Optimization and Molecular Characterization of High potential Dye Degrading Indigenous Microbes from Noyyal

River Basin, Tamilnadu.

6. Category of the Project (Please tick) : R&D

7. Specific Area (Please see Annexure - II) : 3. Environment (Microbiology) 3.1. Bioremediation

8. Duration : 3 Years

9. Total Cost (Rs.) : Rs. 37, 61,000 (Thirty seven lakhs and sixty one thousand rupees)

10. Is the project Single Institutional or Multiple-Institutional (S/M) ? : Single institutional 11. If the project is multi-institutional, please furnish the following: NA 12. Scope of application indicating anticipated product and processes

• Our research focus is to substitute physical and chemical mode of effluent treatment by biological remediation. • This study will give the clear knowledge and characteristics of native organisms

from noyyal river, tamilnau and their dye degrading potential. • It will be cost effective and eco – friendly.

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13. Project Summary (Not to exceed one page. Please use separate sheet) : Enclosed (ANNEXURE- I)

PART II: PARTICULARS OF INVESTIGATORS Principal Investigator : 14. Name : Dr.S.S. Sudha

Date of Birth : 01/05/1972

Sex (M/F) : Female

Designation : Professor& Head

Department : Microbiology

Institute/University : Dr. N.G.P. Arts and Science College (Affiliated to Bharathiar University)

Address : Dr. N.G.P- Kalapatti Road, Coimbatote, Tamilnadu.

PIN: 641048

Telephone : 0422- 2369221, Mob : 09245282174

Fax : 0422-2369206

E-mail : sudhakrish_1972@yahoo.com

Number of Research

projects being handled at present : Nil

Co-Investigator 15. Name : Mr. S. Senthil Prabhu

Date of Birth : 24/07/1980

Sex (M/F) : Male

Designation : Assistant Professor

Department : Microbiology

Institute/University : Dr. N.G.P. Arts and Science College (Affiliated to Bharathiar University)

Address : Dr. N.G.P- Kalapatti Road, Coimbatote, Tamilnadu.

PIN : 64 1048

Telephone : 0422- 2369221, Mob : 09842814320

Fax : 0422-2369206

E-mail : sepisp@yahoo.co.in

Number of Research projects being handled at present: Nil

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PART III : TECHNICAL DETAILS OF PROJECT

16. Introduction

Screening, Optimization and Molecular Characterization of High potential Dye Degrading Indigenous Microbes from Noyyal River Basin, Tamilnadu

India's dye industry produces every type of dyes and pigments close to 80,000 tones.

India is the second largest exporter of dyestuffs and intermediates (developing countries) after

China. The textile industry accounts for the largest consumption of dyestuffs; at nearly 80%.

Industrialization is vital to a nation’s economy because it serves as a vehicle for development.

However, there are associated problems (pollutions and adverse effect) resulting from the

introduction of industrial waste products into the environment.

Textile dyes are chemicals with complex aromatic structures designed to resist the effects

of laundering and sunshine, for example a great number of dyes and other chemicals are used in

textile wet processing. There are more than 105 commercially available dyes with over 1×106 tons

of dyestuff produced annually world-wide. Dyes are a synthetic origin and complex aromatic

molecular structures, which make them stable and difficult to degrade.

The textile industries utilize large volumes of water in its processing operations and

generate substantial quantities of wastewater. Dye wastes are usually discarded into water with or

without processing. Aesthetic merit, gas solubility and water transparencies are affected by the

presence of dyes even in small amounts. The presence of colour in waste water is very important

and usually contributes the major fraction of biochemical oxygen demand. Many classes of

synthetic dyes used in the textile and dyeing industries play a predominant role in almost every

type of application.

The textile factories daily discharge millions of litres of untreated effluents in the forms of

wastewater into public drains that eventually empty into rivers (Olayinka and Alo, 2004). This

alters the pH, increases the biochemical oxygen demand (BOD) and chemical oxygen demand

(COD), and gives the rivers intense colorations. The use of these water resources is limited and

the ecosystem is affected. Colored industrial effluent from the dyeing industries is one more

reason for pollution of aquatic systems which leads major environmental problems due to its

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impact on water bodies and growing public concern over its toxicity and it spoil the agriculture

land too.

Bioremediation using a variety of microbes for the degradation of xeno-biotics seems a

green solution to the problem of environmental pollution. Microbes have been gifted by nature

with the ability of degrading a wide spectrum of environmental pollutants. Microorganisms are

the most abundant organisms in natural freshwater systems and also play a key role in ecological

processes. Detailed knowledge of the diversity and function of microorganisms dwelling in

freshwater habitats is an essential prerequisite for the sustainable management of freshwater

resources. Bacteria, filamentous fungi and microalgae have been extensively evaluated for the

decoloration and degradation of dyes and colored effluents.

Microbes and their enzymes have the potentials to degrade complex and organic

compounds into simpler fragments; sometimes achieving complete mineralization. There has been

an intensive research on microbial decolorization of dye waste waters. Moreover, filamentous

fungi’s extracellular enzymes where Oxidative enzymes (Ferreira et al, 2006) and Bacteria’s

thermostable azoreductase (Maier et al, 2004) plays a significant role in biodegradation of Dyes.

Algae are photosynthetic organisms, which distributed in nearly all parts of the world and in all

kinds of habitats. Alga can degrade number of dyes, postulating that the reduction appears to be

related to the molecular structure of dyes and the species of algae used (Yan and Pan, 2004).

Compared with chemical and Physical methods, biological processes have received more

interest because of their cost effectiveness, lower sludge production and environmental

friendliness. Bioremediation is a cheaper alternative for colour removal in textile effluents.

Decolourization of dye wastewater by microbial metabolic activities and the mixed culture of the

microbes are the subject of many studies which is also our extension but through indigenous

microbial population, a rare prospect also.

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16.1 Origin of the research Proposal

The major and minor rivers flowing in Tamilnadu were the major source for surface and

ground water. One of such rivers flowing in Coimbatore, Erode and Karur districts is the Noyyal

River. The river Noyyal is a seasonal river and it originates from Vellingiri hills in the Western

Ghats of Coimbatore district. It flows through Coimbatore, Erode and Karur districts and finally

joins into Cauvery River near Noyyal village. It flows over a distance of 175 kilometers. The

catchment area of the river is 3.49 lakh hectares. Throughout its distance on both sides of the

banks of river Noyyal, more than 100 villages are situated. Noyyal was the major source for

irrigation, drinking water and other activities of the people living on both sides of the river and

even for people living beyond 3 kms from the river.

The industrial effluents released by dyeing and bleaching factories in Tirupur, a major

hosiery centre in South India has become a serious issue because, it has severe impact on water

bodies. After semi-treatment or without treatment, the effluents are released into Noyyal river. At

present there are about 750 dyeing and bleaching industries in Tirupur. The effluents released by

these units are stored in Orathupalayam Dam, which was constructed in 1991 at the cost of

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Rs. 1646 lakhs. The water-spread area is 1049 acres and it was expected to irrigate 500 acres in

Erode district and 9875 acres in Karur district.

The effluent has altered the nature and quality of both soil and water, which subsequently

and indirectly affect the health and the progeny of human beings. Environmental changes are

inevitable, but at what rate the change is happening will have an impact over the dwellings of

earth because, the drift should provide enough time to adapt, which is not available in the current

problem. This has motivated us to go for indigenous micro- flora that will be a divine force in

striving for welfare of both environment and economy.

Dying industry and garments are purely related to chemistry and Textiles, where the

survival of these firms and its employees has become a big question mark due to the legal and

responsible interference by environmental activists, farmers and guidelines by department of

environment and forestry, government of India. It is essential that everything survives and

environment is protected without fail. Our research purely aims at understanding micro-biota of

Noyyal river basin and discovering the possibilities for biological remediation; it creates a

significant role in installing the knowledge of microbiology for renaissance of both dying and

garment industries.

16.2 (a) Rationale of the study supported by cited literature

Dyes may also significantly affect photosynthetic activity in aquatic life by reducing light

penetration intensity and may also be toxic to some aquatic fauna and flora due to the presence of

aromatics, metals, chlorides, etc. (Dhaneshvar et al., 2007).

Dyes are resistant towards conventional methods of waste water treatment (Joshi et

al.,2004).

The economic and safe removal of the polluting dyes is still an important issue. Because

all the physicochemical methods are very expensive and results in the production of large amount

of sludge, which creates the secondary level of land pollution. In this situation bioremediation is

becoming important, because it is cost effective and environmental friendly and produces less

sludge (Chen et al.,2003).

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Biological process have potential to convert or degrade the pollutant into water, carbon

dioxide and various salts of inorganic nature. The isolation of potent species and there by

degradation is one of the interest in biological aspect of effluents treatment (Mohan et al., 2002).

Therefore, the use of microbial communities for on-site treatment of dye containing waste

waters from textile and dye-stuff industries could be an economical alternative (Ola et al., 2010).

Development of efficient dye degradation requires a suitable strain and its use under

favorable conditions to realize the degradation potential. In recent years there has been an

intensive research on fungal decolorization of dye wastewater. It is turning into a promising

alternative to replace or supplement present treatment processes (Mohandass Ramya et al., 2007).

Most of the Microorganisms have detoxifying abilities (that is, mineralization,

transformation and or immobilization of pollutants), microorganisms, particularly bacteria, play a

crucial role in biogeochemical cycles for sustainable development of the biosphere (Tropel and

Van der Meer, 2004).

Many microorganisms belonging to different taxonomic group of bacteria, fungi,

actinomycetes and algae have been reported for their ability to decolourize azo dyes. Many

bacteria are capable to degrade azo reactive dyes aerobically and anaerobically (Kuhad et al.,

2004).

The presence of extra bands in TLC in decolourized samples indicates the ability of the

bacterium to degrade dyes enzymatically and can be exploited for bioremediation of azo dyes and

their derivates containing wastes, since it seem to have the potential to degrade the toxic azo dyes

into non-toxic product form (Usman Aftab et al., 2011).

Decolorization of textile dyes from fungi Aspergillus Flavus, Fusarium oxysporum,

F.moniliforme and Trichoderma harzianum isolated from the soil samples around the textile

distillery industries of Nanjangud, Karnataka, India were tested their efficacy in decolorization of

textile dyes, Orange 3R, Blue 3R, Yellow GR etc. It was found that all the four fungal species

were found to be efficient in decolouring in textile dyes (Raju et al., 2007).

The potential of Cosmarium species, belonging to green algae, was investigated as a

viable biomaterial for biological treatment oftriphenylmethane dye, Malachite Green (MG). The

results obtained from the batch experiments revealed the ability of algal species in removing dye.

The effects of operational parameters (temperature, pH, dye concentration and algal

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concentration) on decolorization were examined. Optimal initial pH was determined 9 (Daneshvar

et al., 2006).

b) Hypothesis

I) This study is to thorough investigation of high potential dye degrading indigenous microbes

from noyyal river basin .

H1: The indigenous microbes has the high efficiency for dye degradation when individual

Strain.

H2: The consortium of indigenous microbes has high efficiency to dye degradation.

H ∅ : The indigenous microbes of noyyal river basin doesn’t have dye degradation efficiency.

II) This study also investigates the enzymes produced by isolated microbes on effective dye

degradation.

H1 : The enzymes from indigenous microbes posses high efficiency in dye degradation

H ∅ : The enzymes from indigenous microbes are not efficient in dye degradation.

C) Key questions

1. Why this proposed research is important?

2 How many bacterial strains are having high efficiency on dye degradation from

noyyal river basin, Tamilnadu?

3. How many fungal strains are having high efficiency on dye degradation from

noyyal river basin ?

4. How many microalgae can effectively degrade the dyes from noyyal river basin?

5. At what extent mixed cultures (consortium) of native microbes will degrade the dyes?

6. Will the enzymes from native microbes has the high potential to dye degradation?

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16.5 Current status of research and development International status

Wastewater effluents from the textile and other dye-stuff industries contain

significant amounts of synthetic dyes that require treatment to prevent groundwater

contamination. In research aimed at biotechnology for treatment of azo dyes, this study examined

4 strains of azo-dye degrading bacteria to identify efficient strains and determine incubation times

required for decolorization (Mohammad Reza et al.,2011).

The potential of Trametes villosa and Pycnoporus sanguineus to decolorize reactive textile

dyes used for cotton manufacturing in the State of Minas Gerais, Brazil, was evaluated. Growth

and decolorization halos were determined on malt extract agar containing 0.002g L-1 of the

dye(Katia et al., 2006).

Textile effluent-adapted bacteria were isolated from effluent samples collected from the

discharge and drainage pipes of three textile industries in the Oshodi/Isolo Local Government

area of Lagos, Nigeria. The textile effluent non-adapted bacteria were isolated from soil samples

taken from a municipal landfill. As a preliminary step in the development of textile effluent

biotreatment processes involving indigenous microbes, we have discovered textile effluent

adapted strains of Acinetobacter and Bacillus, and effluent non-adapted Bacillus species with

potential use in effluent treatment. (Olukanni et al., 2006).

Practical development of bacteria for bioaugmentation requires careful screening that is

based not only on their efficacy in pure culture, but also on their ability to compete with the

indigenous microbial communities in wastewater streams and ability to be produced and delivered

as a stable inoculum. (Azeem Khalid et al.,2010).

Activated sludge samples collected from wastewater treatment plant (WWTP) in textile

factory “Giorgetti Bulgaria” AD were exploited for isolation of dye decolorizing bacteria. A

microbial strain AZO29 was selected based on its efficiency, showing maximum and faster

decolourization of textile dye Amaranth. Identification of isolate by 16SrRNA technique revealed

that the microorganism were from family Pseudomonadaceae of Gammaproteobacteria (99%

confidence), the closest matches found after BLAST alignment were 16S sequences of

Pseudomonas strains (97% similarity) (Grekova-Vasileva et al., 2009).

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The industrial manufacturing of azo dyes and textile finishing processes generate

wastewater streams contaminated with azo dyes. Some of the azo dyes are difficult to treat by

conventional wastewater treatment methods. Compared with physical and chemical methods,

biological techniques are preferable because of economical advantages and low possibility of by

products production. Screening potential microorganisms is a critical step in the construction of

an effective remediation system (Usman Aftab et al., 2011).

Biological processes have potential to convert or degrade the pollutant in water into

carbon dioxide and various salts of inorganic nature. (Hazrat Ali et al., 2009).

Biological methods of treatment combined with physical or chemical methods or both for

colour removal are immensely useful and cost effective. It is therefore imperative to study the

metabolic and enzymatic systems of fungi in order to gain insight into their degradation

metabolisms. Amongst the fungi studied so far, wood degrading white-rot fungi has been reported

to have the potential to successfully treat coloured industrial effluents (Moturi et al.,2009).

In recent years, the ability of microorganisms to decolorize textile wastewater has received

great attention due to the environmental persistence and toxicity of these pollutants. In this paper

biological decolorization of triphenylmethane dye,C.I. Basic Green 4 (BG 4), by Chlorella

species was investigated. The effect of operational parameters (temperature, pH, initial dye

concentration and algal concentration) on decolorization efficiency was examined (Kahtee et

al.,2009).

National Status

Biological decolourization has been investigated as a method to transform, degrade or

mineralize azo dyes. In the present studies bacteria from soil from dye waste area were subjected

for acclimatization to C.I. Reactive yellow 145, an azo dye in the basal nutrient media.The most

promising bacterial isolate was used for further dye degradation studies. The 16s r RNA gene

sequencing revealed the isolated organism as Enterococcus faecalis strain YZ66. A preliminary

identification of the culture was based upon biochemical tests. 16sr-RNA sequencing of the

isolated organism was done in GeneOmbio Technologies Pvt. Ltd., Pune, India. Phylogenetic tree

was constructed by the Neighbour –Joining method (Sahasrabudhe and Pathade et al., 2011).

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A number of biotechnological approaches have been suggested by recent research as of

potential interest in combating this pollution source in an eco- efficient manner. The ligninolytic

enzymes of white-rot fungi (e.g., Mn peroxidases, lignin peroxidases, and laccases,) are directly

involved not only in the degradation of lignin in their natural lignocellulosic substrates, but also in

the degradation of various xenobiotic compounds including dyes (Indira Priyadarshini et

al.,2011).

The Pleurotus sajor caju silver nanoparticle effectively decolorized the dye within

24hours of incubation when compared with its plain culture (Pleurotus sajor caju) which takes

more than 48 hours for the same process.This is for the first time reporting that Pleurotus sajor

caju silver nanoparticle was used for the decolorization of the dye Congo red (Nithya and

Ragunathan,2011).

Isolation and identification of dye decolourizing fungal isolates from textile dye effluent

was carried out. The isolates of Aspergillus flavus, Aspergillus niger, Helminthosporium sp.,

Mucor sp. and Penicillium sp. were isolated from the textile effluent samples collected from

Elampillai Jalagandapur, Tamilnadu . Different parameters were used for optimizing. Conditions

for maximum decolourization effect by the fungal isolate (Ponraj et al.,2011).

The study describes degradation ability of A.tamaraii, mixed fungal culture (Trichoderma

sp. and A.flavus) and Penicillium purpurogenum on various synthetic dyes.A.tamaraii decolorized

>90% of Coomassie brilliant blue, Phenol blue, and malachite green dyes (Ramalingam et al.,

2010).

Decolourisation of textile dyes from fungi Aspergillus flavus, Fusarium oxysporum,

Fusarium moniliforme and Trichoderma harzianum isolated from the soil samples around the

textile distillery industries of Nanjangud, Karnataka, India were tested for their efficacy in

decolourisation of textile dyes (Raju et al., 2007).

Frequently encountered thirteen fungi isolated from dye contaminate soils were assessed

for their decolourisation and biodegradation of three sulphonated azo dyes. The fungal

organisms showed 41-80% decolourisation of scarlet red and 80-90% of fast greenish blue and

48-89 % of brilliant violet. For the decolourisation the responsible enzymes, K-amylase, protease

and catalase were estimated. A. niger showed maximum secretion of K-amylase in 14 days of

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incubation. The protease production was ranged from 25-38 U/ml by majority of the fungi tribe

T. notatum was found to be efficient in production of catalase. This study is the fungal based

treatment system for the reclamation of the dye contaminated soils (Laxminarayana, 2010).

Development of efficient dye degradation requires a suitable strain and its use under

favourable conditions to realize the degradation potential. In recent years there has been an

intensive research on fungal decolorization of dye wastewater. It is turning into a promising

alternative to replace or supplement present treatment processes (Mohandass Ramya et al., 2007).

16.6 The relevance and expected outcome of the proposed study

This study is to provide the high potential dye degrading organisms from noyyal river basin and

molecular characterization is to give clear knowledge of high efficiency dye degrading Indigenous

organisms. So this study will give the solution for major environmental problem & solution for dyeing

industries with cost effective and eco friendly method.

16.7 Preliminary work done so far

Studies on decolorization of textile dyes by white rot fungi- Trametes hirsute (2010).

The Study studies on white rot fungi Trametes hirsute given the greatest extent in

decolorization of Blue RR, Blue FNG, and Black dyes. Various parameters have been optimized

and dye degradation is analysed by GCMS. Blue RR was decolorized at 35o C, and at pH 4

using maltose as a carbon source and Yeast extract as nitrogen source. Blue FNG was

decolorized at 30o C and pH 3 using maltose as carbon source and yeast extract as nitrogen

source. Black dye was effectively decolorized at 35o C at pH 4 using glucose as carbon source

and yeast extract as nitrogen source. The laccase enzyme production from above fungi were

well optimized of for different parameters. Decolorization of dyes with Immobilized beads of

crude enzyme (laccase) extract was also observed and dye degradation was analysed by

GCMS. Along with other fungi like Aspergillus fumigates, Penicillium sp. and Fusarium

oxysporum were also used for degradation of various dyes.

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17. Objectives

Ø Collection of samples from noyyal river basin and industrial effluents. Ø Isolation and Screening of dye degrading potential organisms. Ø Identification of Bacteria, fungi and microalgae. Ø Molecular based Characterization of Isolates. Ø Optimization of culture condition and enzymes for effective dye degradation.

Ø Effect of mixed culture (consortium) on biodegradation. Ø Toxicity studies on plants, animals and microbes. Ø The field trial will be conducted using the enzymes and cell biomass.

18. Work Plan (Year-wise Plan of work and targets to be achieved)

18.1 Methodology 1st year 1.1Collection of sample

Soil, water samples from noyyal river basin, effluents from industries will be collected and

samples are transport to the laboratory.

1.2 Isolation of Organisms

Bacterial isolation by serial dilution on nutrient agar and maintain on same medium. Soil

Fungi will be isolated by serial dilution and plating on PDA (potato Dextrose Agar) medium.

Microalgae isolation and it will be subcultured on growth (MN) medium. (Khataee et al., 2009).

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2nd Year 2.1 Screening of organisms Screening of the bacterial isolates in Bushnell and Hass medium amended with different

concentrations of dyes, (Prachi et al., 2009). The fungal isolates to be screened for dye

degrading activity (Subramanyan, 1971). The dye degrading efficiency for isolated microalgae

will be checked by growth medium ( Incubation in glass jar with growth medium t 25 ο C for 15

days and exposed in light) amended with dye (Khataee et al., 2009) .

2.2 Decolorization assay

Decolorization activity will be analysed by UV-VIS Spectrophotometer (Moorthi et al.,

2007, Nehra et al., 2008).

2.3 Identification of Screened Organisms

The identification of bacterial isolates by gram staining, morphology, biochemical

methods and other standard microbiological methods (Cappuccino and Sherman, 1999). The

identification of fungal taxa will be made based on the Manual of Soil Fungi (Gilman, 1957);

2002, Rafi M, and Sjjad – Ur-Rahman, 2002). Hyphomycetes (Subramanyan, 1971); Dematiceous

and More Dematiceous Hyphomycetes (Ellis, 1971); Identification of cyanobacteria by using the

standard taxonomic manuals of Geitler (1932) and Desikachary (1959).

2.4 Molecular characterization

16S rRNA gene sequencing, plasmid profiling will be done based on standard protocol.

(Azcarate-Peril and Raya,2001).

2.5 Enzyme assay The enzymes from isolates to be assayed by using UV spectrophotometer. (Hatvani and Mecs, 2001, Shanmugam et al.,1999, Moturi and Singara charya, 2009).

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2.6 Optimization Optimization of physicochemical characters like temperature, pH, Carbon sources,

Nitrogen sources for potential dye degradation by the cultures and enzymes (Rajendran et al.

2011, Ponraj et al., 2011).

2.7 Estimation of protein by lowry’s method. 2.8 Design and development of Microbial consortium & immobilization Various combinations of isolated native microbes to developing consortium by applying

Cayley’s table (Rajendran et al.,2011). Immobilized cells also will be used to analysis the dye

degradation potential (Ohmomo et al. 1987; Shin et al. 2002).

2.9 Biodegradation activity analysis After completion of degradation, the decolorized medium will be analyzed in HPLC (High

performance liquid chromatography), FTIR (Fourier Transform Infrared Spectroscopy) UV-VIS

Spectrophotometer (Faisal et al.,2006).

3rd Year 3.1 Toxicity studies Phytotoxic studies on plants, Microbial toxicity by zone of inhibition method and toxicity

of both treated and untreated dyes will be checked on various animals and aquatic organisms

(Puwaneswari et al.,2006; Subhasini Sharma et al.,2007).

3.2 Field trails

The high potential dye degrading organisms, Mixed cultures, and enzymes will be applied

in bioreactors, industrial effluent treatment plants and the contaminated sites.

3.3 Report Preparation The results obtained will be interpreted by statistical analysis, finally the report preparation with documental evidences.

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18.2 Connectivity of the participating institutions and investigators (in case of multi- institutional projects only) : Not Applicable 18. 3. Alternate strategies (if the proposed experimental design or method does not work what is the alternate strategy) : Molecular cloning (recombinant technology) will be applied to isolated organisms for effective degradation of dyes. 19. Timelines: (Please provide quantifiable outputs) Period of study Achievable targets 0-6 Months Collection of review

6-12 Months Collection of samples and Isolation

12-18 Months Screening , identification and Molecular characterization

18-24 Months Optimization by different parameters for enzymes and cell biomass for dye degradation

24-30 Months Toxicity studies, Effect of consortium on dye degradation

30-36 Months Field trial (application) and report preparation

20. Name and address of 5 experts in the field

Sr.No. Name Designation Address 1. Dr. R. Rajendran Associate

professor Department of Microbiology, PSG College of Arts and Science College, Coimbatore- 14.Tamilnadu.

2. Dr.S. Rajakumar Assistant professor

Department of Marine Biotechnology Bharathidasan University, Trichirapalli-24, Tamilnadu

3. Dr.P.M. Ayyasamy Assistant professor

Department of Microbiology, Periyar University Salem-11, Tamilnadu

4. Dr.P..Lakshmanaperumalsamy Registrar Karpagam University, Coimbatore-21. Tamilnadu

5. Dr. K. Selvam Head & professor

Department of Biotechnology, Dr.N.G.P.Arts & Scince College, Coimbatore- 48, Tamilnadu

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Budget (In Rupees)

PART IV: BUDGET PARTICULARS

A. Non-Recurring (e.g. equipments, accessories, etc.)

S. No. Item Year 1 Year 2 Year 3 Total 1. Phase contrast microscope 1,50,000 1,50,000 2. HPLC 8,00,000 8,00,000 3. Gradient PCR 4,00,000 4,00,000 4 Books & Journals 1,20,000 1,20,000

Sub-Total (A) = 14,70,000 B. Recurring B.1 Manpower (See guidelines at Annexure-III)

S. No. Position No.

Consolidated Emolument

Year 1 Year 2 Year 3 Total

1. Project fellow Rs.12,000 /m 1-2 Yrs Rs. 14,000/m 3rd yer

1,44,000 1,44,000 1,68,000 4,56,000

Sub-Total (B.1) =4,56,000 B.2 Consumables

S. No.

Item Qty Year 1 Year 2 Year 3 Total

1. Chemicals, Reagents - 90,000 1,50,000 80,000 3,20,000 2. Glass wares - 75,000 75,000 60,000 2,10,000 3. Molecular characterization

kit - - 2,00,000 - 2,00,000

4. Animals for toxicity Studies

- 1,00,000 1,00,000

Sub-Total (B.2) =8,30,000

Other items Consolidated Emolument

Year 1 Year 2 Year 3 Total

B.3 Travel & field work

1,00,000 75,000 1,00,000 2,75,000 B.4 Contingency & hiring - 75,000 75,000 80,000 2,30,000 B.5 Overhead

- 5,00,000 Sub-total of B (B.1+B.2+B.3+B.4+B.5)

22,91,000

Grand Total (A + B) 37,61,000

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PART V : EXISTING FACILITIES Resources and additional information

1. Laboratory:

a. Manpower Lab assistants: 2 (no’s)

b. Equipments

S.No Name of the Instrument

1. Autoclave vertical (350 X 550 mm)

2. UV – VIS Spectrophotometer

3. Electrophoresis apparatus

4. High Speed Centrifuge

5. Laminar air flow chamber

6. Incubator

7. BOD Incubator

8. Rotary Evaporator

9. Ultra Cooling Centrifuge

10. Shaker

11. Colorimeter

12. UV Transilluminator

13. pH Meter

14. Magnetic Stirrer

15. Hot plate

16. Incubatory Shaker

17. Deep freezer

18. Clinical centrifuge

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19. Hot air oven

20. Qubec colony counter

21. Membrane filter unit with vaccum pump

22. Soxhlet apparatus

2. Other Infrastructural facilities:

Animal House, Fungal Room, Incubation room, Research Laboratory, Sterilization and Decontamination rooms.

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PART VII: PROFORMA FOR BIOGRAPHICAL SKETCH OF INVESTIGATORS Provide the following information for the key personnel in the order listed on PART II. Follow

this format for each person. Principal Investigator

Name : Dr. S.S. Sudha Designation : Professor & Head

Department/Institute/University : Microbiology/ Dr. N.G.P. Arts & Science College/ Bharathiar

University

Date of Birth: 01/05/1972 Sex (M/F); Female (39yrs) SC/ST: NA

Education (Post-Graduation onwards & Professional Career)

Sl No. Institution Place

Degree Awarded

Year Field of Study

1. Kandasamy Kandar College, Paramathy Vellur

M.Sc 1999 Microbiology

2. AVVM Sri Pushpam College, Thanjavur

PhD 2006 Microbiology

3. Bharathiar University, Coimbatore

MBA 2010 Hospital Management

A. Position and Honors Position and Employment (Starting with the most recent employment)

Sl No. Institution Place

Position From (Date) To (date)

1. Dr.N.G.P. Arts & Science College, Coimbatore

Professor & Head 01/06/2006 Till date

2. Shrimathi Indira Gandhi College, Trichy

Asst. Professor 01/06/2004 20/05/2005

3. Kurinji college of arts and Science, Trichy

Asst. Professor 01.06.1999 30.05.2004

Honors/Awards : 1. Acted as chair person for the technical session in International Conference on

Bioresource Technology organized by Department of Botany Nirmala college for Women on Antihyperlipidemic activity of spirulina species in diet induced albino rats. During 7 & 8th October 2010.

2. Acted as chair person for judging projects presented on National Children’s

Science Congress by Coimbatore district school students at GRD College on

12th November 08.

3. Invited speaker in Coimbatore Management Association Weekly Meeting on

24.11.2008 and delivered a speech on “Disaster Management. Professional Experience and Training relevant to the Project : NA

22

B. Publications (Numbers only) : 35 Books: NA Research Papers, Reports: 35 General articles:..... Patents: NA Others (Please specify) NA Selected peer-reviewed publications (Ten best publications in chronological order)

1. S.S.Sudha and Parvathy A. “A study on Screening for L-Asparaginase Producing

Microorganism from Beach soil” Research J of Biol. Sci.: II .1, 2010 : 50-54.

2. Uma P., and S S Sudha “Biodecolorization of textile Reactive Dyes using Trametes

hirsute”. Research J of Biol. Sci. Vol : I No.1, 2009: 30-38.

3 S S Sudha. “Marine Environment: A Potential Source for L-Asparaginase Producing Microorganism” The Icfai University Journal of Biotechnology. Vol : 3 No.4, 2009 :57-62.

4.S.Kalaivani, A.MahalakshmiPriya, S.S.Sudha and S.Balasubramaniyan

Phycoremediation of sewage using Microalgae Chlorella sp. Nature

Environment and Pollution Technology.Vol 8, No1, 2009: 187-192.

5. Vimalin J, S.S. Sudha, D. Padmanabhan, A. Asarudeen, P. Ayyappan. Isolation &

Characterization of community acquired Methicillin Resistant

Staphylococcus aureus in school going children. Res J Biol Sci.

2009;1(3): 153-56.

6. R. Vijayakumar, S.S. Sudha, A. Pannerselvam, T Thaajuddin. A comparative

study on cultivation and nutritional status of Oyster Mushroom Pleurotus

ostreatus on different substrates. J.Sci.Trans.Environ.Technov, 2008.

1(4):209-212.

7. S.S.Sudha,A.Panneerselvam and N.Tajuddin “Antagonistic interaction of some

Mangrove Soil Fungi against plant pathogens-Curuvlaria lunata and

Drechslera ellisii” (J.Sci. Trans.Environ.Technov., 1(3):154-157, 2008.

8. S.S.Sudha,A.Panneerselvam and N.Tajuddin “Seasonal variation of cyanobacteria at

Muthupet mangrove environs, Tamilnadu,South India.( Seaweed Res.Utiln.,29

(1&2): 263-271,2007).

23

9. S.S.Sudha,A.Panneerselvam and N.Tajuddin “Antagonistic Potentials of

Mangrove Soil Fungi against plant pathogens” Indian Journal of Applied

Microbiology, 6(1): 61-65,2006).

10. S.S.Sudha,A.Panneerselvam and N.Tajuddin.“Antagonistic effect of some mangal

soil fungi against Colletotrichum falcatum went. and Curvelaria pallescens

(Walker) Boedijn” .Geobios32, 288-290,2005 .

11. S.S.Sudha,A.Panneerselvam and N.Tajuddin .“Antagonistic Potential of some

Mangrove Soil Fungi against Fusarium oxysporum and Rhizoctonia solani”

J.Microb. World 7(2) PP.140-143, 2005..

Publications relevant to the proposed area of work

Publications

Ø Uma P., and S S Sudha “Biodecolorization of textile Reactive Dyes using Trametes

hirsute”. Research Journal of Biological Science. Vol : I No.1, 2009: 30-38.

Ø S.S.Sudha and Parvathy A. “A study on Screening for L-Asparaginase Producing

Microorganism from Beach soil” Research Journal of Biological Science. Vol : II

No.1, 2010 : 50-54.

Ø S.S.Sudha,A.Panneerselvam and N.Tajuddin “Seasonal variation of cyanobacteria at

Muthupet mangrove environs, Tamilnadu,South India.( Seaweed Res.Utiln.,29 (1&2):

263-271,2007).

Ø S.S.Sudha,A.Panneerselvam and N.Tajuddin “Antagonistic interaction of some

Mangrove Soil Fungi against plant pathogens-Curuvlaria lunata and Drechslera

ellisii” (J.Sci. Trans.Environ.Technov., 1(3):154-157, 2008).

Ø R. Vijayakumar, S.S. Sudha, A. Pannerselvam, T Thaajuddin. A comparative study

on cultivation and nutritional status of Oyster Mushroom Pleurotus ostreatus on

different substrates. J.Sci.Trans.Environ.Technov, 2008. 1(4):209-212.

24

Ø S.Kalaivani, A.MahalakshmiPriya, S.S.Sudha and S.Balasubramaniyan

Phycoremediation of sewage using Microalgae Chlorella sp. Nature Environment and

Pollution Technology.Vol 8, No1, 2009: 187-192.

Biographical sketch of Co- Investigator

Name : Mr. S. Senthil Prabhu Designation : Assistant Professor

Department/Institute/University : Microbiology/ Dr. N.G.P. Arts & Science College/ Bharathiar

University

Date of Birth: 24/07/1980 Sex (M/F) ; Male (30yrs) SC/ST: NA

Education (Post-Graduation onwards & Professional Career)

Sl No. Institution Place

Degree Awarded

Year Field of Study

1. Periar University, Salem M.phil 2008 Microbiology

2. G.R.Damodaran College of Science, coimbatore

M.Sc 2005 Microbiology

3. Dr.N.G.P. arts and science college, coimbatore

B.Sc 2001 Biochemistry

A. Position and Honors Position and Employment (Starting with the most recent employment)

Sl No. Institution Place

Position From (Date) To (date)

1. Dr.N.G.P. Arts & Science College, Coimbatore

Assistant Professor 01/06/2008 Till date

2. Nehru arts and science college, Coimbatore

Asst. Professor 01/06/2006 30/04/2008

Honors/Awards : Professional Experience and Training relevant to the Project : NA

B. Publications (Numbers only) : 06 Books: NA Research Papers, Reports: 05 General articles:01 Patents: NA Others (Please specify) NA

25

26

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Annexure - I

Project Summary

This study is to isolate bacteria, fungi and microalgae by various microbiological

methods. The isolated organisms are screened for dye degradation potential. Screened

organisms are subjected to molecular characterization, 16s rRNA sequencing after

polymerase chain reaction (PCR) and this to construct the phylogenetic tree, plasmid

profiling of screened organisms also will be done. The Enzymes from isolates will be used

for determination of potential dye degradation, this enzymes will be estimated by Lowry et al

method. Optimization of enzymes and cell mass by different physiological and biochemical

parameters for efficient dye degradation. The immobilized cells will be used to determine the

dye degradation potential. Toxicity studies will be conducted on plants, microbes and

animals. Finally above things will be practically applied to the fields as well as bioreactors in

Tirupur dying industries, and contaminated sites of noyyal river paves, its a way for natural

remediation for environment and remedy for dyeing industries.