Prof. Richard Christopherson. Left-right: Dr Larissa Belov, Prof Richard Christopherson, Dr Ben...
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Transcript of Prof. Richard Christopherson. Left-right: Dr Larissa Belov, Prof Richard Christopherson, Dr Ben...
Cancer Proteomics Group
Prof. Richard Christopherson
Left-right: Dr Larissa Belov, Prof Richard Christopherson, Dr Ben Crossett (SUPRU), Munther Alomari (PhD), Duthika Mallawaaratchy (PhD), Jerry Zhou (PhD),
Kieran Matic (Honours), Yiping Che (PhD), Dr Kim Kaufman, Trisha Almazi (PhD)
Missing: Dr Swetlana Mactier, Erin Sykes (PhD).
Colorectal cancer (CRC)LeukaemiaMelanomaGlioblastoma (GBM)
Cancers we study:
Identification of proteome changes in cancer cells after treatment with a drug such as:
◦ Purine analogues (e.g., fludarabine) ◦ HSP90 inhibitors (e.g., SNX-7081)◦ ER-stress inducers (e.g., thapsigargin)
Understand mechanisms of drug action and resistance, identification of new drug targets
Anti-cancer drugs
DotScan™ CD antibody microarrays◦ Capture live cells and obtain a surface profile
(disease signature, immunophenotype)◦ In collaboration with major NSW hospitals, we
have collected and tested many different clinical samples, including:
Leukaemias Colorectal cancers Metastatic melanoma
◦ To improve disease stratification, prognostication and prediction of response to treatment
Cell surface markers
Stage III melanoma Survival analyses – prognostic biomarkers good versus poor prognosis groups with
identical stage cancers
Glioblastoma multiformeSerial lesions, pre and post chemotherapy, biomarkers for chemo-resistance
Chronic lymphocytic LeukaemiaStable vs progressive disease course
Comprehensive, quantitative clinical proteomics
Clinical samples and cell lines In vitro chemotherapy treatment Flow cytometry Sub-cellular proteome isolation
◦ Cytosol, nucleus, mitochondria and plasma membrane
Mass Spectrometry and gel-based analyses (SUPRU)
Quantitative analysis◦ DIGE, iTRAQ, SRM and
label-free methods
Techniques
Thank you!
Christopherson lab