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Prof. Dr. Xiaoxun Xie 2006.10 What is Histology ? a science which studies normal microstructures &...
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Transcript of Prof. Dr. Xiaoxun Xie 2006.10 What is Histology ? a science which studies normal microstructures &...
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Prof. Dr. Xiaoxun Xie2006.10
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What is Histology ?a science which studies normal microstructures & relationship between these structures and functions.
What is Tissue ? cells with their ground substance acting together performing particular functions.
What is organ? organized by multiple tissues in particular ways.
What is system? formed by several organs with related functions.
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Molecules
Organelles
Cells
Tissues
Organs
Systems
Relative proportion & arrangement of tissue types determine the organ’s structure & capabilities.
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Microscopy: Light microscopy (LM)
Electron microscopy (EM)• Transmission electron microscope (TEM)• Scanning electron microscope (SEM)
Staining: H&E Histochemistry immunohistochemistry In situ hybridiztion …
Methodology in Histology
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TEM
SEM
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Heamatoxylin: Base dye; blue color; combine with the acidic components
Eosin: Acidic dye; Red color; combine with the basic components
• basophilic• acidophilic (eosinopilic)• neutrophilc
nucleolus
nucleus
H&E ------ Routine staining for LM
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H&E Staining
Hematoxylin(Base dye)
Eosin(acid dye)
Stain :NucleusRERRibosome…
* Basophilia
Stain:CytoplasmLysosomeMitochodriaCollagen F…
*Acidophilia
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Epithelial Tissue Tissue
Two basic types– Covering epithelia
outer layer of the skin
inner surface of cavities and vessels;
ducts of the exocrine glands– Glandular epithelia
* main function for secretion
* make up most of the glands
Other types: sensory /germinal epithelia
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Name Distribution
Covering Epi. Surface of body, luminal organs & cavity organs
Glandular Epi. Glands (secretion part)
Sensory Epi. Sensory organs
Myoepithelium Basal portion of the glands
Germinal Epi. Sexual glands
Type and distribution of epitheliumType and distribution of epithelium
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- Protection;
- Absorption;
- Lubrication;
- Excretion and Secretion;
- Sensory reception;
- Reproduction.
Functions of epithelium
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Free (apical) Surface
Basement Membrane
C.T.
Characteristics of Epithelia
* Highly cellular with little intercellular space
* Polarity
* Avasculation: no blood vessels
* Supported by underlying connective tissue
* High regeneration capacity
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Free, basal and lateral surfaces of epi.cells display specific structural modifications
Polarity
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Classification of coveringClassification of covering EpitheliaEpithelia
based on following features:
- Number of cell layer
one layer: simple epi.
several layers: stratified epi.
- Shape of cells on the apical surface
flat: squamous epi.,
square: cuboidal epi.,
rectangular: columnar epi.,
- Specializations of cell surfaces: cilia
exception: transitional epi..
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Classification depends on layer
Simple layerSimple layer Stratified layerStratified layer
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Classification depends on cilia
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•one layer of flat cells with one flat nucleus
•found in: -alveoli of the lungs, -kidney glomeruli, -heart, blood and lymphatic vessels -ventral body cavities.
• thinnest of all - gas diffusion - filtration - exchange of waste & nutrients filtration
Simple squamous epithelium
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Simple squamous epithelium
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1. Nuclei (flat and parallel to the surface of the epithelium)
2. Face to lumen
21
1
1
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The entire circulatory system is lined by a S. S. Epi. that is called endothelium.
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Mesothelium
The major body cavities ( peritoneal, pleural, pericardial) are lined by S. S. epi that is called mesothelium.
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serosa ( 浆膜)( mesothelium + CT )
lining thoracic cavity & covering the lungs----------pleura (胸膜)
lining abdominal cavity & covering the abdominal organs -----peritoneum (腹膜)
enclosing the heart -----pericardium (心包膜)
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- Cuboidal cells with one round nucleus
- surface view: six sides-hexagonal
- distribution:
small glands,
kidney tubules,
ovary surface, ect.
Simple Cuboidal Epithelium
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Simple Cuboidal EpitheliumSimple Cuboidal Epithelium
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columnar cells with one oval nucleus
• Two subtypes:
-non-ciliated:
*microvilli on its apical surface.
*found in digestive tract, ducts of some glands.
*function: absorption of digested food products; secretion of mucus, enzymes, ect.
- ciliated: * cilia on its apical surface.* found in uterine and uterine tubes.
Simple Columnar Epithelium
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Pseudostratified ciliated columnar epithelium
Different types of cells
Columnar cell----------------------Goblet cell ---------------------------------------------
Fusiform cell------------------------
Basal cell--------------------------------
All cells rest on the BM
Not all can reach to the free surface
cilia in columnar cell
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Differences between stratified & pseudostratified
• Appears layers but all cells touch the basement membrane
B.M.
Stratified Pseudostratified
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1. Nuclei2. Epithelium3. Cilia 4. Lumen
Pseudostratified ciliated columnar epithelium
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Stratified EpitheliumStratified Epithelium
Stratified squamous: the outer layer of skin
Stratified cuboidal and columnar: large ducts of glands
Transitional: lining of urinary organs can stretch as the bladder fills
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Stratified Squamous Epithelium
* Multi-layer cells
* Squamous cells in outermost layers
* Large/polygonal cells in intermediate regions
* cells in deep: cuboidal / low columnar resting on the BM
* two types: -Non-keratinised (mucous)
-Keratinised (skin)
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Keratinization: a process by which the superficial cells die and become tough, hard squama.
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* to accommodate distension and stretching of organ.
* Lining of urinary bladder and ureter.
Transitional Epithelium
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Specializations of Epithelial Cells
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- Glycocalyx: glycoprotein containing coat
- Cilia
- Microvilli
- Stereocilia: very long microvilli (immotile)
Specializations in cellular apical surface
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Cilia
• elongated, motile
structures;
• 0.3~0.5 µm (diameter);
• 5~10 µm long.
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KARTEGENER’S SYNDROME
• Absence of dynein arms• Respiratory difficulty• Males sterile- flagellum of sperm is modified ciliu
m
Plasma Membrane
Microtubule Doublet
Dynein
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Microvilli
• "brush border" ( LM)• Finger-like projection(EM)• 0.1 µm in diameter• 1 µm long
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Brush border
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MICROVILLI STRUCTURE
• covered by the cell membrane
• microfilaments in the core
• microfilaments interact
with terminal web
ZA
Microvilli
Cell membrane
visible network of horizontal actin filaments on the apical of an epithelial cell.
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Microvilli Cilia
appearance Small and short Big & long
observation EM LM
Surface Cell-membrane Cell-membrane
Axes Cytoplasm Cytoplasm
Microfilament MicrotubuleBasal portion Terminal web Basal body
Function Increase surface area Movement
Difference between microvilli & cilia
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Plasma membrane
Microvilli-with core filaments
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Specializations in cellular lateral surface
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* at the apical border of the cell;
* outer layers of plasma membrane fuse together;
* forming a waterproof belt
surrounding the cell;
* serving as a barrier;
* Preventing diffusion of
molecules between cells.
Tight junction (zonula occluden)
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* located just below the tight j. as a 'belt'
* intercellular space (15-20 nm) with an adhering
substance (glycoprotein)
* microfilaments associated with
cytoplasmic surface of epi. cell
* Function in mechanical
attachment
Intermediate Junction (Zonula Adherens)
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Tonofilaments--------------
Attachment plaque------
Desmosome (Macula Adherens)
• Spot-like
• intercellular space 25-30 nm with a intermediate line
• attachment plaques in cytoplasmic surface
• tonofilaments
intermediate line
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Gap junction
Connexon-------------
* Narrow intercellular space spanned by connexon
* A low-resistance pathway between cells
* Not only in the epithelial cells.
Cell membrane
Gap / Communicating Junction
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Gap Junction
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3 or 4 junctions are collectively termed junctional complexes.
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Pemphigus
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Specialization of the cellular basal surface
• Hemi-desmosome
• Basal infolding
• Basement membrane
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Basal infoldings show the increased surface area available for the ion and water transport.
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Basement membrane
• Amorphous material on LM• Two layers on EM• Function as connection and semi permeable membrane
Hemidesmosome
Basal Lamina
Reticular lamina
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Glandular Epithelium
• A type of epithelial tissue whose primary function is secretion, and is the prominent tissue forming glands.
A specialization of epithelial tissue to secrete substances. It may consist of a single cell (unicell) or a multicellular arrangement.
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Classified into two types of gland:– endocrine - internal secretion: produce hormones– exocrine - external secretion : secrete into body
cavities or the body surface i.e. tears, sweat, saliva
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General structure of exocrine gland
• Secretory unit (acius)- serous cell- mucous cell
•duct
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“TYPICAL” EXOCRINE GLAND STRUCTURE
Secretory Portion
Duct
Surface Epithelium
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WHAT IS CONNECTIVE TISSUE(C.T)?
a basic type of tissue which provides structural
& metabolic support for other tissues and organs.
WHERE IS CONNECTIVE TISSUE FROM?
All C.T develop from mesenchyme (embryonal connective tissue).
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General characteristics of C.T.
• relatively small number of cells without polarity
• intercellular material is the dominant component
• enrichment of blood and lymphatic vessels
• same origination-----from mesenchyme
• Function as
connecting, supporting, nutrition, defense, repair.
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Umbilical Cord, human - H&E
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Components of Connective Tissues
• Cells
• intercellular material---dominant component
matrix--- amorphous ground substance
• polysaccharide-rich macromolecules
•Tissue fluids
• ions
fibers: extracellular filamentous structure
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Classification of Connective Tissue
Connective tissue proper
• Loose connective tissue (L.C.T)
• Dense Connective Tissue (D.C.T.)
• Adipose tissue
• Reticular tissue
Special connective tissue
• Cartilage
• Bone
• Blood
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Introduction of L.C.T• most common type of C.T;
• most widely distributed in the body;
• sponge-like;
• greater variety of cells and fiber than other C.T;• flexible but not very resistant to stretching;
• Function as
-binding other tissue to each other;
-nutrients providing;
-wounding healing and destruction foreign matter.
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A stretched preparation of L.CT. The pink fibers of different thicknesses are collagenous (or white) fibers. The dark, thin, more tortuous fibers are elastic (or yellow) fibers. Most of the nuclei belong to fibroblasts.
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Component of L.C.T.
Cells Fibers Ground substance
Fibroblast Collagen glycosaminoglycan (GAG)
Macrophage Elastic proteins
Plasma cell Reticular tissue fluid
Mast cell
Fat cells (adipocyte)
Undifferentiated cell
Wandering cell
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Ground substance
• jelly-like substance , no definite shape
• contains: * proteoglycan Protein + glycosaminoglycan (GAG)
chondroitin sulphatekaratin sulphatehyaluric acid
* glycoprotein
• function as* diffusion of nutrients & metabolic waste* physical barrier
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FIBROBLAST
Origin: mesenchymal cells LM:
Appearance: long flat cell with filopodia
Nucleus: oval and pale with fine chromatin; conspicuous nucleoli;
Cytoplasm: nearly homogenous,
weakly basophilic
Fibroblast fibrocyteinacitve
older
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Fibroblast & Fibrocyte
fibroblast ——
fibrocyte——————
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EM: rich in RERs, free ribosomes,
well-developed Golgi apparatus
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Function:
involving in the synthesis
and secretion of :
* Proteins related to production of collagenous, reticular, and elastic fibres.
* Ground substances
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MACROPHAGE
Origin: monocytes
LM:
• irregular in shape
• blunt processes
(pseudopodia)
• acidophilic cytoplasm with
phagocytosing material
• inactive macrophages may look like fibroblasts
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FIBROBLAST
Origin: mesenchymal cells LM:
Appearance: long flat cell with filopodia
Nucleus: oval and pale with fine chromatin; conspicuous nucleoli;
Cytoplasm: nearly homogenous,
weakly basophilic
Fibroblast fibrocyteinacitve
older
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Fibroblast & Fibrocyte
fibroblast ——
fibrocyte——————
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EM: rich in RERs, free ribosomes,
well-developed Golgi apparatus
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Function:
involving in the synthesis
and secretion of :
* Proteins related to production of collagenous, reticular, and elastic fibres.
* Ground substances
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MACROPHAGE
Origin: monocytes
LM:
• irregular in shape
• blunt processes
(pseudopodia)
• acidophilic cytoplasm with
phagocytosing material
• inactive macrophages may look like fibroblasts
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EM* irregular surface with numerous finger-like projections* numerous lysosomes * many phagocytic vacuoles
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Function of macrophages•phagocytosis and destruction of foreign bodies
•immune response
- capture, process and re-secrete antigen
- transfer antigen into lymphocytes
- stimulate immune response
•secrete bioactive products
lysosome emzyme
chemotatic factors for neutrophils
growth factors…
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PLASMA CELL
Origin: B lymphocytesLM:
Appearance: round or ovoid
Cytoplasm: always basophilia ( ribosomal RNA)
pale stained perinuclear region
Nucleus: located eccentrically
clock-face chromatin arrangement
or wood-cart-wheel nucleus
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FIBROBLAST
Origin: mesenchymal cells LM:
Appearance: long flat cell with filopodia
Nucleus: oval and pale with fine chromatin; conspicuous nucleoli;
Cytoplasm: nearly homogenous,
weakly basophilic
Fibroblast fibrocyteinacitve
older
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Fibroblast & Fibrocyte
fibroblast ——
fibrocyte——————
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EM: rich in RERs, free ribosomes,
well-developed Golgi apparatus
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Function:
involving in the synthesis
and secretion of :
* Proteins related to production of collagenous, reticular, and elastic fibres.
* Ground substances
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MACROPHAGE
Origin: monocytes
LM:
• irregular in shape
• blunt processes
(pseudopodia)
• acidophilic cytoplasm with
phagocytosing material
• inactive macrophages may look like fibroblasts
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MAST CELLS
LM:Appearance: fairly large,
round, ovoid.Nucleus: non-lobulated spherical,
pale staining.Cytoplasm:
many coarse granules stains with basic dyes but exhibit metachromasia.
Phennomenon in which a basic dye changes color from blue to red/purple due to the dense presence of polyanionic groups (i.e. phosphates sulfates)
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EM
• undulant surface folds • many secretion granules (very heterogeneous) containing:
*histamine
*heparin
*SRS-A
*other chemical
mediators
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Function of mast cells
Involving in allergic reaction Antigen (Ag) IgE produced by plasma cell
binding to mast cells re-exposed to same Agre-exposed to same Ag
interacting with mast cell surface-bound IgE triggering triggering
Mast cell degranulation affecting various tissues/organsaffecting various tissues/organs
• increasing vascular permeability, edema• smooth muscle contraction
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FAT CELLS ( ADIPOCYTES )
• cytoplasm is filled with lipid droplets
• a typical thin rim cytoplasm
• flattened nucleus is eccentric.