8/3/2019 priyanshi

1/4

PROJECT

For

Structural Biology and SecondaryMetabolites

2011-2012

Submitted By: Submitted To:

Name: PRIYANSHI GIRI Dr. Pankaj.K.Mishra

Programme: Msc. Biotechnology

Email: priyanshi.giri@gmail.com

Mob.: 8826267823

Date of Submission:11-11-2011

Department of Biotechnology

School of Engineering and Technology

8/3/2019 priyanshi

2/4

Sharda University, Greater Noida

Research Article 01

SECTION A (1.5)

Title: Cardiac differentiation of embryonic stemcells by substrate immobilization of insulin-likegrowth factor binding protein 4 with elastin-likepolypeptides

Name of Authors:Ayaka Minato a, Hirohiko Ise b,*,Mitsuaki Goto c, Toshihiro Akaike b

Affiliation:

a) Graduate School of Bioscience andBiotechnology, Tokyo Institute of Technology, B-57,4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japanb )Frontier Research Center, Tokyo Institute of

Technology, 4259 Nagatsuta-cho, Midori-ku,Yokohama 226-8503, Japanc )Celagix Res Ltd., 5800-3 Nagatsuta-cho, Midori-

ku, Yokohama 226-0026, Japan

Name of Journal: BIOMATERIALS

Impact Factor: 7.882

Year in which article published: 2011

Volume: 33

8/3/2019 priyanshi

3/4

Issue: 2012

Page No.:515-523

SECTION B (1.5)

Objectives:

i.a useful strategy for cardiovascular regenerativemedicine.

ii. we report a strategy for cardiomyocytedifferentiation ofESCs using substrate immobilization of insulin-likegrowth factor binding protein 4 (IGFBP4) withelastin-like polypeptides.

SECTION C (1.5)

Statistical model used for data analysis:Cellexperiments were performed in triplicate, and theresults were expressed asmean _ standard deviation (SD). Students t testwas used to assess the statistically significant

differences in the results. A probability (p) value of

8/3/2019 priyanshi

4/4

SECTION D (1.5)

Summary: It was indicated that IGFBP4 was stablyimmobilized to polystyrene dishes by the fusion of(GVGVP)67 because the number of GVGVP repeatsneeded to be more than 67 in order to keep thehydrophobic interaction with the dishes at 37 _C.Moreover, it was suggested that (GVGVP)67-IGFBP4 that was immobilized to the dishes could

inhibit the Wnt/b-catenin signaling more stronglyand continuously than IGFBP4 as a solublefactor.These results demonstrated thatcardiomyocyte differentiation of ESCs waseffectively promoted by strong and continuousinhibition of Wnt/b-catenin signaling with(GVGVP)67-IGFBP4. Substrate immobilization ofsoluble factors with elastin-like polypeptides might

lead to the development of useful strategies forregenerative medicine of various tissues such asheart, liver, and neuron.