Prion Project - Update Mass Spectrometry of Prions Andy Lo, Béla Reiz, Messele Fentabil Dr. Liang...

Prion Project - UpdateMass Spectrometry of Prions

Andy Lo, Béla Reiz, Messele Fentabil

Dr. Liang Li

Department of Chemistry

University of Alberta

Mass Spectrometry (MS)

Mass Spectrometry is an instrumental method used to identify chemical substances by means of the separation of their gaseous ions using magnetic and/or electric fields according to their mass-to-charge ratio.

MS in PROTEOMICS

Molecular weight of proteins

Masses of peptides in a mixture

Masses of fragment ions of a peptide

Experimental Procedure

Protein

Digestion (Peptides)

LC-MALDIMS

MS

MSMS

MS

Database Search

Protein MS


Protein


LC-MALDIMS

MS

MSMS

MS

Database Search

PrP Tryptic Digest - MS

PrP&Myo Tryptic Digest - MS


Protein


LC-MALDIMS

MS

MSMS

MS

Database Search

PrP&Myo Tryptic DigestLC-MALDI-MS


Protein


LC-MALDIMS

MS

MSMS

MS

Database Search

PrP&Myo Tryptic DigestLC-MALDI-MSMS


Protein


LC-MALDIMS

MS

MSMS

MS

Database Search

Database Search Results

PrP Chymotryptic DigestLC-MALDI

PrP Chymotryptic DigestResults

Future Work

Chemical surface modification of surface exposed residues using:

N-bromosuccinimide (NBS) for Trp exposed residues

Hydroxylation of surface exposed Cys, Trp, Tyr, Met

Thanks!

Dr. Liang Li Andy and Messele All Li Group members

Prion Project - Update Mass Spectrometry of Prions Andy Lo, Béla Reiz, Messele Fentabil Dr. Liang...

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