Principles of Automated Hematology
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Lesson 2-10 Principles of Automated Hematology
Transcript of Principles of Automated Hematology
History of Cell Counting
• 1855, hemacytometers• Manual counts until 1950s• Coulter
– Automated counter (1956)– Principle of electrical impedance– Early counters only performed RBC and WBC
counts
Two Basic Technologies• Electrical-impedance counting
– Electrolyte solution– Aperture, impedance– Improvements
RBC Parameters• RBC indices
– Mean cell volume [MCV]– Mean cell hemoglobin [MCH]– Mean cell hemoglobin concentration [MCHC]
• Cytochemical staining• Image processing• Flow cytometry
Automated Differential Counting
Five-part differential