Prime Pro qPCR reagent kits - Techne · 2020. 9. 23. · TKIT08001 TKIT08001M DNA, Brucella abortus...

Prime Pro qPCR reagent kits

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Techne offer a range of over 400 Prime Pro DNA/RNA pathogen

detection kits for a wide variety of applications. The kits contain all of the

components required to run 150 tests. To use the kits, simply combine the

components and introduce your DNA/RNA before placing into your real

time PCR instrument. For your convenience, each kit is available with and

without the mastermix component. The kit components are lyophilised

(freeze dried) so can be shipped and stored at ambient temperature. They

are stable for 18 months. Suitable for:

qPCR reagent kits from Techne

Pathogen Internal control Negative control Positive control Interpretation

+ + - +

+ - - +

- + - + Negative result

- - - -

+ + + +

DNA Results

Step Time Temperature

Enzyme activation 10 minutes 95ºC

Denaturation 10 seconds 95ºC

Data collection* 60 seconds 60ºC

DNA Protocol

* Fluorogenic data for the pathogen and Beta-actin endogenous control is collected during this step through the FAM channel. Fluorogenic data for

the internal extraction control should be collected during this step through the VIC channel (or the Cy5 channel if using a Roche LightCycler 1.0 or 1.5)

• Molecular Biology research

• Hospitals

• Research

• Food agencies

• Water testing

• Standards and testing agencies

• Health agencies

• Defence agencies

• Veterinary/Agriculture

• Parasitology

• For general laboratory and

research only

Plate setup includes:

1. Internal control DNA (read through VIC) and samples (read through FAM).

2. Beta-actin endogenous control (read through VIC).

3. Positive control, also used for standard curve (read through FAM).

4. Negative control (read through FAM).

DNA KitsEach Prime Pro DNA reagent kit employs the same experimental setup, amplification protocol and interpretation of results.

Experiment fail

Positive result

50 cycles

1 cycle

RNA KitsEach Prime Pro RNA reagent kit employs the same experimental setup, amplification protocol and interpretation of results.

Pathogen Internal control Negative control Positive control Interpretation

+ + - +

+ - - +

- + - + Negative result

- - - -

+ + + +

RNA Results

RNA Protocol Step Time Temperature

Reverse transcription 10 minutes 55ºC

Enzyme activation 8 minutes 95ºC

Denaturation 10 seconds 95ºC

Data collection* 60 seconds 60ºC

• Kits packed in a convenient foil pouch

• Lyophilised - (freeze dried)

o No dry ice shipping required

o Reduced shipping costs

o Ship to hot climates

o Ship at the weekend

• Simple protocol, identical for each kit

• 150 reactions per kit.

o Low cost per test

• qPCR can be days faster, more accurate and more

quantifiable than traditional microbiological methods

• Suitable for any qPCR instrument with FAM/VIC detection

Advantages

• Lyophilised primer and probe mix

• Lyophilised master mix

• Lyophilised master mix resuspension buffer

• Lyophilised ROX Ampule

• High-copy DNA for standard curve

• Internal extraction control DNA

• Beta-actin endogenous control

• RNAse/DNAse free water

• Reverse transcriptase (RNA kits only)

Kit contents

Experiment fail

Positive result

* Fluorogenic data for the pathogen and Beta-actin endogenous control is collected during this step through the FAM channel. Fluorogenic data for

the internal extraction control should be collected during this step through the VIC channel (or the Cy5 channel if using a Roche LightCycler 1.0 or 1.5)

50 cycles

1 cycle

Plate setup includes:

1. Internal control DNA (read through VIC) and samples (read through FAM).

2. Beta-actin endogenous control (read through VIC).

3. Positive control, also used for standard curve (read through FAM).

4. Negative control (read through FAM).

Without WithMastermix Mastermix Description

Respiratory InfectionsTKIT10005 TKIT10005M DNA, Adenovirus Type B

TKIT10006 TKIT10006M DNA, Adenovirus Type C

TKIT10007 TKIT10007M DNA, Adenovirus Type F&G

TKIT10003 TKIT10003M DNA, Ajellomyces capsulata

TKIT11016 TKIT11016M DNA, Chlamydophila pneumoniae

TKIT11017 TKIT11017M DNA, Chlamydophila psittaci

TKIT11015 TKIT11015M DNA, Cryptococcus neoformans

TKIT10028 TKIT10028M DNA, Enterobacter cloacae

TKIT11023 TKIT11023M DNA, Geosmithia argillacea

TKIT10044 TKIT10044M RNA, H1N1 influenza

TKIT11026 TKIT11026M RNA, Avian Influenza A virus Subtype H7N9

TKIT10040 TKIT10040M DNA, Haemophilus influenzae

TKIT10046 TKIT10046M DNA, Human Bocavirus genomes

TKIT10049 TKIT10049M RNA, All Group 1 Coronavirus genomes

TKIT10050 TKIT10050M RNA, All Group 2 Coronavirus genomes

TKIT10035 TKIT10035M RNA, Influenza Type A M1

TKIT10036 TKIT10036M RNA, Human Influenza Type A M2

TKIT10043 TKIT10043M RNA, Human Influenza A virus Subtype H1

TKIT10045 TKIT10045M RNA, Human Influenza A virus Subtype H3

TKIT10037 TKIT10037M RNA, Human Influenza Type B

TKIT10064 TKIT10064M RNA, Human Metapneumovirus

TKIT10066 TKIT10066M RNA, Human Parainfluenza virus Type 1



TKIT10069 TKIT10069M RNA, Human Parainfluenza virus Type 4A

TKIT10070 TKIT10070M RNA, Human Parainfluenza virus Type 4B

TKIT10082 TKIT10082M DNA, Human Polyomavirus 6



TKIT10085 TKIT10085M RNA, Human Rhinovirus Subtype 14


TKIT10087 TKIT10087M RNA, Human Rhinovirus Subtype 1B



TKIT10090 TKIT10090M RNA, Human Rhinovirus All subtypes (generic)

TKIT10097 TKIT10097M DNA, Klebsiella pneumoniae

TKIT07012 TKIT07012M DNA, All Legionella species

TKIT10102 TKIT10102M DNA, Legionella pneumophila

TKIT11027 TKIT11027M DNA, Leptospirosis

TKIT10114 TKIT10114M DNA, Merkel cell polyomavirus

TKIT10115 TKIT10115M DNA, Methicillin-resistant Staphylococcus aureus

TKIT10108 TKIT10108M DNA, Moraxella catarrhalis

TKIT08017 TKIT08017M DNA, Mycobacterium avium subspecies

paratuberculosis

TKIT10113 TKIT10113M DNA, Mycobacterium tuberculosis complex

TKIT11044 TKIT11044M DNA, Mycobacterium avium

TKIT10113 TKIT10113M DNA, Mycobacterium tuberculosis complex

TKIT10112 TKIT10112M DNA, Mycoplasma pneumoniae

TKIT10133 TKIT10133M RNA, Respiratory Syncytial virus

TKIT10134 TKIT10134M RNA, Respiratory Syncytial virus A

TKIT10135 TKIT10135M RNA, Respiratory Syncytial virus B

TKIT11035 TKIT11035M RNA, SARS coronavirus

TKIT10100 TKIT10100M DNA, Legionella longbeachae

TKIT07017 TKIT07017M DNA, Simkania negevensis

TKIT10155 TKIT10155M DNA, WU polyomavirus

TKIT10098 TKIT10098M DNA, KI polyomavirus

Sexually transmitted InfectionsTKIT10016 TKIT10016M DNA, Candida albicans

TKIT10021 TKIT10021M DNA, Chlamydia trachomatis

TKIT10039 TKIT10039M DNA, Haemophilus ducreyi

TKIT07008 TKIT07008M RNA, Hepatitis A virus

TKIT10047 TKIT10047M DNA, Hepatitis B virus

TKIT10091 TKIT10091M DNA, Herpes simplex Type 1 (HHV1)

TKIT10092 TKIT10092M DNA, Herpes simplex Type 1 and 2 (HHV1&2)


Human pathogen detection kits form the largest part of

the Prime Pro reagent range. This segment includes kits for

pathogenic bacteria, viruses, protozoa and parasites.

• Respiratory infections

• Sexually transmitted infections

• Herpes infections

• Hepatitis viruses

• Human papillomavirus

• Gastrointestinal infections

• Vector-borne diseases

• Meningitis

• Periodontal infections

• Influenza H1N1

Human pathogens


TKIT10062 TKIT10062M RNA, Human Immunodeficiency virus Type 1

TKIT10063 TKIT10063M RNA, Human Immunodeficiency virus Type 2

TKIT10071 TKIT10071M DNA, Human Papillomavirus 11






TKIT10077 TKIT10077M DNA, Human Papillomavirus 52 and 52b



TKIT10110 TKIT10110M DNA, Mycoplasma hominis

TKIT10119 TKIT10119M DNA, Neisseria gonorrhoeae

TKIT10149 TKIT10149M DNA, Treponema pallidum

TKIT10150 TKIT10150M DNA, Trichomonas vaginalis

TKIT10154 TKIT10154M DNA, Ureaplasma urealyticum

Herpes viral InfectionsTKIT10024 TKIT10024M DNA, Cytomegalovirus (HHV5)

TKIT10030 TKIT10030M DNA, Epstein Barr virus (HHV4)




TKIT10057 TKIT10057M DNA, Human Herpesvirus 6


TKIT10058 TKIT10058M DNA, Human Herpesvirus 6 variant A

TKIT10059 TKIT10059M DNA, Human Herpesvirus 6 variant B



Hepatitis InfectionsTKIT07008 TKIT07008M RNA, Hepatitis A virus

TKIT10047 TKIT10047M DNA, Hepatitis B virus

TKIT10051 TKIT10051M RNA, Hepatitis C virus

TKIT10054 TKIT10054M RNA, Hepatitis Delta virus

TKIT07009 TKIT07009M RNA, Hepatitis E virus

Human PapillomavirusTKIT10079 TKIT10079M DNA, Human Papillomavirus 6






TKIT10077 TKIT10077M DNA, Human Papillomavirus 52 and 52b



Gastrointestinal infectionsTKIT11006 TKIT11006M DNA, Aeromonas hydrophila

TKIT11003 TKIT11003M DNA, Ancylostoma duodenale

TKIT07001 TKIT07001M DNA, Bifidobacterium bifidum

TKIT06001 TKIT06001M DNA, Bifidobacterium longum

TKIT11002 TKIT11002M DNA, Blastocystis genus (All species)

TKIT08004 TKIT08004M DNA, Campylobacter Coli

TKIT08005 TKIT08005M DNA, Campylobacter Jejuni

TKIT10016 TKIT10016M DNA, Candida albicans

TKIT07004 TKIT07004M DNA, All Clostridium perfringens species

TKIT10017 TKIT10017M DNA, Clostridium difficile (toxin A)

TKIT10018 TKIT10018M DNA, Clostridium difficile (toxin B)

TKIT11018 TKIT11018M DNA, Cryptosporidium

TKIT08003 TKIT08003M DNA, Cyclospora cayetanensis

TKIT07002 TKIT07002M DNA, Bacillus cereus E33

TKIT10013 TKIT10013M DNA, All Bacteroides species

TKIT10012 TKIT10012M DNA, Balamuthia mandrillaris

TKIT10029 TKIT10029M DNA, Entamoeba histolytica

TKIT10032 TKIT10032M DNA, All Entamoeba species

TKIT10028 TKIT10028M DNA, Enterobacter cloacae

TKIT10027 TKIT10027M DNA, Enterococcus caseliflavus

TKIT07006 TKIT07006M DNA, Enterococcus faecalis

TKIT07007 TKIT07007M DNA, Enterococcus faecium

TKIT08013 TKIT08013M DNA, Enteropathogenic Escherichia coli

TKIT08008 TKIT08008M DNA, Escherichia coli

TKIT08007 TKIT08007M DNA, Escherichia coli 0157:H7

TKIT08009 TKIT08009M DNA, Escherichia coli O104:H4

TKIT08015 TKIT08015M DNA, Giardia intestinalis

TKIT10041 TKIT10041M DNA, H Pylori

TKIT10046 TKIT10046M DNA, Human Bocavirus

TKIT08016 TKIT08016M DNA, Listeria monocytogenes


TKIT11031 TKIT11031M RNA, Rotavirus A

TKIT11032 TKIT11032M RNA, Rotavirus B

TKIT11033 TKIT11033M RNA, Rotavirus C

TKIT08018 TKIT08018M DNA, Salmonella enterica

TKIT08010 TKIT08010M DNA, Shiga toxin (stx1) producing Escherichia coli

TKIT08011 KIT08011M DNA, Shiga toxin (stx2b) producing Escherichia coli

TKIT05001 TKIT05001M DNA, All pathogenic Salmonella species

TKIT07020 TKIT07020M DNA, All Vibrio species

TKIT02002 TKIT02002M DNA, Toxigenic subspecies of Vibrio cholerae

TKIT07019 TKIT07019M DNA, All Vibrio cholerae subspecies

TKIT02003 TKIT02003M DNA, Yersinia enterocolitica

TKIT10121 TKIT10121M DNA, Oxalobacter formigenes

Vector-borne diseasesTKIT11004 TKIT11004M DNA, African Trypanosomiasis

TKIT11007 TKIT11007M DNA, Anaplasma phagocytophilum

TKIT10008 TKIT10008M DNA, Borrelia afzelii

TKIT10009 TKIT10009M DNA, Borrelia burgdorferi

TKIT10011 TKIT10011M DNA, Borrelia garinii

TKIT10023 TKIT10023M RNA, Chikungunya virus

TKIT08002 TKIT08002M DNA, Coxiella burnetii

TKIT08006 TKIT08006M RNA, Crimean-Congo Haemorrhagic Fever virus

TKIT10025 TKIT10025M RNA, Dengue virus

TKIT10026 TKIT10026M RNA, Dengue virus Type 3

TKIT10031 KIT10031M DNA, All Ehrlichia species

TKIT08014 TKIT08014M DNA, Francisella tularensis

TKIT01001 TKIT01001M QPCR Kit, RNA, Japanese Encephalitis virus

TKIT10099 TKIT10099M DNA, Leishmania infantum and donovani

TKIT10101 TKIT10101M DNA, Leishmania major

TKIT10103 TKIT10103M DNA, Leishmania tropica

TKIT10104 TKIT10104M DNA, Lactobacillus genus

TKIT10105 TKIT10105M DNA, All Leishmania species

TKIT10107 TKIT10107M DNA, Lyme disease

TKIT10122 TKIT10122M DNA, Plasmodium falciparum

TKIT10129 TKIT10129M DNA, Plasmodium ovale

TKIT10130 TKIT10130M DNA, Plasmodium vivax

TKIT10126 TKIT10126M DNA, Plasmodium knowlesi

TKIT10127 TKIT10127M DNA, Plasmodium malariae

TKIT10131 TKIT10131M DNA, All Plasmodium species

TKIT10132 TKIT10132M DNA, Rickettsia (All species)

TKIT10141 TKIT10141M RNA, Sandfly Fever Sicilian virus

TKIT10152 TKIT10152M RNA, Tick-borne Encephalitis virus

TKIT10145 TKIT10145M DNA, Trypanosoma cruzi

TKIT12080 TKIT12080M DNA, Trypanosoma equiperdum

TKIT11043 TKIT11043M RNA, Wesselsbron virus

TKIT01003 TKIT01003M RNA, West Nile virus

TKIT01002 TKIT01002M RNA, Western equine encephalomyelitis virus

TKIT10156 TKIT10156M RNA, Yellow Fever virus

MeningitisTKIT10024 TKIT10024M DNA, Cytomegalovirus (HHV5)

TKIT10120 TKIT10120M DNA, All Neisseria meningitidis

TKIT10033 TKIT10033M RNA, All Human Enterovirus species

TKIT10030 TKIT10030M DNA, Epstein Barr virus (HHV4)

TKIT10040 TKIT10040M DNA, Haemophilus influenzae





TKIT11038 TKIT11038M DNA, Streptococcus pneumoniae


Periodontal infectionsTKIT10001 TKIT10001M DNA, Aggregatibacter actinomycetemcomitans

TKIT10123 TKIT10123M DNA, Porphyromonas gingivalis

TKIT10124 TKIT10124M DNA, Prevotella intermedia

TKIT10140 TKIT10140M DNA, Streptococcus mutans

TKIT11040 TKIT11040M DNA, Streptococcus salivarius

TKIT10147 TKIT10147M DNA, Tannerella forsythia

TKIT10146 TKIT10146M DNA, Treponema denticola

OthersTKIT10004 TKIT10004M DNA, All Acanthamoeba species

TKIT10002 TKIT10002M DNA, Acinetobacter baumannii

TKIT11005 TKIT11005M DNA, Aspergillus fumigatus

TKIT11008 TKIT11008M DNA, Aspergillus

TKIT03001 TKIT03001M DNA, Bacillus anthracis

TKIT11009 TKIT11009M DNA, Bartonella henselae

TKIT08001 TKIT08001M DNA, Brucella abortus

TKIT07003 TKIT07003M DNA, Brucella genus (All species)

TKIT10010 TKIT10010M DNA, Burkholderia cepacia complex

TKIT11001 TKIT11001M DNA, Burkholderia mallei (with Mastermix)

TKIT11010 TKIT11010M DNA, Burkholderia pseudomallei

TKIT11012 TKIT11012M DNA, Campylobacter fetus

TKIT11013 TKIT11013M DNA, Campylobacter fetus subspecies venerialis

TKIT10022 TKIT10022M RNA, Chaoyang virus

TKIT11011 TKIT11011M DNA, Chlamydophila abortus

TKIT10017 TKIT10017M DNA, Clostridium difficile (toxin A)

TKIT10018 TKIT10018M DNA, Clostridium difficile (toxin B)

TKIT11019 TKIT11019M DNA, Clostridium tetani

TKIT10019 TKIT10019M DNA, Corynebacterium diphtheriae toxin A&B

TKIT11020 TKIT11020M RNA, Dobrava-Belgrade virus

TKIT11021 TKIT11021M DNA, Enterocytozoon bieneusi

TKIT11022 TKIT11022M DNA, All Encephalitozoon species


TKIT10038 TKIT10038M DNA, Fungi Kingdom (including Yeast)

TKIT10055 TKIT10055M RNA, Hand, foot and mouth disease

TKIT10033 TKIT10033M RNA, All Human Enterovirus species

TKIT10065 TKIT10065M RNA, Human Measles virus

TKIT10080 TKIT10080M QPCR Kit, DNA, Human Parvovirus B19

TKIT10081 TKIT10081M DNA, Human polyomavirus 12

TKIT10094 TKIT10094M RNA, Human T-lymphotropic virus Type I

TKIT10095 TKIT10095M RNA, Human T-lymphotropic virus Type 2

TKIT10096 TKIT10096M DNA, Klebsiella oxytoca

TKIT10097 TKIT10097M DNA, Klebsiella pneumoniae

TKIT06015 TKIT06015M DNA, Lactobacillus plantarum


TKIT10107 TKIT10107M DNA, Lyme disease

TKIT10114 TKIT10114M DNA, Merkel cell polyomavirus

TKIT10115 TKIT10115M DNA, Methicillin-resistant Staphylococcus aureus

TKIT10116 TKIT10116M DNA, MRSA Staphylococcal cassette chromosome

TKIT10117 TKIT10117M DNA, MRSA (SCC mec) Type Iva

TKIT10118 TKIT10118M RNA, Mumps virus

TKIT10111 TKIT10111M DNA, Mycobacterium marinum and ulcerans

TKIT10110 TKIT10110M DNA, Mycoplasma hominis

TKIT11028 TKIT11028M DNA, Mycoplasma species

TKIT07014 TKIT07014M DNA, All Naegleria species

TKIT12070 TKIT12070M DNA, Orf virus (Contagious pustular dermatitis)

TKIT10125 TKIT10125M DNA, Pneumocystis jirovecii

TKIT10128 TKIT10128M DNA, Proteus mirabilis

TKIT07016 TKIT07016M DNA, Pseudomonas aeruginosa

TKIT11030 TKIT11030M RNA, Rabies virus

TKIT10136 TKIT10136M RNA, Rubella virus

TKIT03003 TKIT03003M RNA, Reston ebola virus

TKIT10139 TKIT10139M DNA, Serratia marcescens

TKIT10144 TKIT10144M DNA, Simian virus 40

TKIT10142 TKIT10142M RNA, Sin Nombre virus

TKIT10143 TKIT10143M DNA, Saint Louis Polyomavirus

TKIT02001 TKIT02001M DNA, Staphylococcus aureus

TKIT10137 TKIT10137M DNA, Staphylococcus epidermidis

TKIT10138 TKIT10138M DNA, Staphylococcus haemolyticus

TKIT11034 TKIT11034M DNA, Streptococcus agalactiae

TKIT11036 TKIT11036M QPCR Kit, DNA, Streptococcus mitis

TKIT11037 TKIT11037M DNA, Streptococcus oralis

TKIT11038 TKIT11038M QPCR Kit, DNA, Streptococcus pneumoniae

TKIT11039 TKIT11039M QPCR Kit, DNA, Streptococcus pyogenes

TKIT11040 TKIT11040M QPCR Kit, DNA, Streptococcus salivarius

TKIT11041 TKIT11041M QPCR Kit, DNA, Streptococcus sanguinis

TKIT03005 TKIT03005M RNA, Sudan Ebola virus

TKIT03006 TKIT03006M RNA, Taï Forest Ebola virus

TKIT11042 TKIT11042M DNA, Toxoplasma gondii

TKIT10153 TKIT10153M DNA, Trichodysplasia spinulosa polyomavirus

TKIT10148 TKIT10148M DNA, Tsukamurella inchonensis

TKIT03002 TKIT03002M RNA, Zaire ebola virus

Primers and probes provide highly specific, easy

to interpret test results with sensitivity down to

10 copies.

High sensitivityInternal extraction control confirms that the DNA

extraction process was successful. Exogenous

DNA is spiked into the lysis buffer.

Internal controlPositive control confirms all conditions and

reagents are working well. A high-concentration

standard is used as positive control.

Positive control

• Endogenous control confirms quality of biological sample.

• Beta-actin confirms that a valid biological template was extracted.

• A primer and probe mix is included to detect Beta-actin through the VIC channel.

• Poor Beta-actin signal may indicate the sample did not contain sufficient biological material.

Endogenous control


AvianTKIT10002 TKIT10002M DNA, Acinetobacter baumannii

TKIT11004 TKIT11004M DNA, African Trypanosomiasis


TKIT12033 TKIT12033M DNA, Avian adenovirus EDS76 Egg Drop Syndrome

TKIT12055 TKIT12055M QPCR Kit, RNA, Avian Infectious Bronchitis virus (IBV)

TKIT12049 TKIT12049M RNA, Avian Influenza A virus Subtype H5





TKIT12004 TKIT12004M RNA, Avian orthoreovirus

TKIT12014 TKIT12014M DNA, Beak and Feather Disease virus


TKIT11001 TKIT11001M DNA, Burkholderia mallei




TKIT12023 TKIT12023M DNA, Chicken anemia virus


TKIT12030 TKIT12030M DNA, Columbid herpes virus 1



TKIT12032 TKIT12032M DNA, Duck Hepatitis B virus




TKIT12045 TKIT12045M DNA, Fowlpox virus

TKIT12046 TKIT12046M DNA, Gallid herpesvirus 1

TKIT12047 TKIT12047M DNA, Gallid herpesvirus 2

TKIT11025 TKIT11025M RNA, Bird flu H5N1


TKIT12054 TKIT12054M RNA, Infectious Bursal Disease virus (IBDV)


TKIT12064 TKIT12064M DNA, Microsporum gypseum

TKIT11044 TKIT11044M DNA, Mycobacterium avium

TKIT10106 TKIT10106M DNA, Mycobacterium leprae & Mycobacterium

lepromatosis

TKIT12063 TKIT12063M DNA, Mycoplasma gallisepticum

TKIT12069 TKIT12069M RNA, Newcastle disease virus

TKIT12071 TKIT12071M DNA, Ornithobacterium rhinotracheale

TKIT11029 TKIT11029M DNA, Pasteurella multocida

TKIT03004 TKIT03004M RNA, Rift Valley Fever virus







TKIT08011 TKIT08011M DNA, Shiga toxin (stx2b) producing Escherichia coli

TKIT08012 TKIT08012M DNA, Tellurite resistant Escherichia coli

BovineTKIT11004 TKIT11004M DNA, African Trypanosomiasis

TKIT12001 TKIT12001M DNA, Anaplasma centrale

TKIT12003 TKIT12003M DNA, Anaplasma marginale


TKIT12006 TKIT12006M DNA, Babesia bigemina

TKIT12007 TKIT12007M DNA, Babesia bovis

TKIT12013 TKIT12013M DNA, Babesia divergens

TKIT03001 TKIT03001M DNA, Bacillus anthracis


TKIT12017 TKIT12017M RNA, Bluetongue virus

TKIT12018 TKIT12018M RNA, Bluetongue virus 1


TKIT12015 TKIT12015M DNA, Bovine herpesvirus 1

TKIT12016 TKIT12016M RNA, Bovine Leukemia virus

TKIT12020 TKIT12020M RNA, Bovine Viral Diarrhoea virus

TKIT08001 TKIT08001M DNA, Brucella abortus













TKIT12044 TKIT12044M RNA, Foot and Mouth Disease virus




paratuberculosis

TKIT12058 TKIT12058M DNA, Mycoplasma bovis

TKIT12067 TKIT12067M DNA, Mycoplasma mycoides cluster










TKIT12078 TKIT12078M DNA, Theileria annulata

TKIT12084 TKIT12084M DNA, Theileria mutans

TKIT12085 TKIT12085M DNA, Theileria parva

TKIT12083 TKIT12083M DNA, Trichophyton mentagrophytes

TKIT12082 TKIT12082M DNA, Tritrichomonas foetus

TKIT12081 TKIT12081M DNA, Trypanosoma evansi

Veterinary and agriculture kits are a fast growing

part of the Prime Pro qPCR reagent range,

currently addressing many challenges in the

environment.

Veterinary and Agriculture



Ovine/caprineTKIT11004 TKIT11004M DNA, African Trypanosomiasis

TKIT12003 TKIT12003M DNA, Anaplasma marginale



TKIT12017 TKIT12017M RNA, Bluetongue virus





TKIT12022 TKIT12022M DNA, Capripoxvirus












paratuberculosis

TKIT12067 TKIT12067M DNA, Mycoplasma mycoides cluster

TKIT12073 TKIT12073M RNA, Peste-des-petits-ruminants virus







TKIT12076 TKIT12076M DNA, Sheep Poxvirus





EquineTKIT04001 TKIT04001M RNA, African Horse Sickness virus

TKIT11004 TKIT11004M DNA, African Trypanosomiasis

TKIT12009 TKIT12009M DNA, Babesia caballi

TKIT11002 TKIT11002M DNA, Blastocystis genus





TKIT12035 TKIT12035M DNA, Equid Herpesvirus 1



TKIT12038 TKIT12038M RNA, Equine infectious anemia virus








TKIT12095 TKIT12095M DNA, Strongylus vulgaris

TKIT12079 TKIT12079M DNA, Theileria equi

TKIT12080 TKIT12080M DNA, Trypanosoma equiperdum

TKIT12081 TKIT12081M DNA, Trypanosoma evansi


FelineTKIT11004 TKIT11004M DNA, African Trypanosomiasis



TKIT11009 TKIT11009M DNA, Bartonella henselae

TKIT12009 TKIT12009M DNA, Babesia caballi


TKIT12008 TKIT12008M DNA, Bordetella Bronchiseptica & Bordetella

Parapertussis

TKIT12025 TKIT12025M DNA, Chlamydophila felis



TKIT12039 TKIT12039M RNA, Feline coronavirus

TKIT12040 TKIT12040M RNA, Feline calicivirus

TKIT12042 TKIT12042M DNA, Feline Herpesvirus

TKIT12043 TKIT12043M RNA, Feline Immunodficiency virus

TKIT12041 TKIT12041M RNA, Feline Leukemia virus





TKIT12062 TKIT12062M DNA, Mycoplasma felis

TKIT12065 TKIT12065M DNA, Mycoplasma haemofelis







TKIT11042 TKIT11042M DNA, Toxoplasma gondii


TKIT12082 TKIT12082M DNA, Tritrichomonas foetus

CanineTKIT11004 TKIT11004M DNA, African Trypanosomiasis




TKIT12008 TKIT12008M DNA, Bordetella Bronchiseptica & Bordetella

Parapertussis

TKIT12021 TKIT12021M DNA, Canine Babesiosis

TKIT12024 TKIT12024M RNA, Canine Distemper virus

TKIT12026 TKIT12026M DNA, Canine herpes virus

TKIT12028 TKIT12028M RNA, Canine Norovirus

TKIT12090 TKIT12090M RNA, Canine parainfluenza virus






TKIT10099 TKIT10099M DNA, Leishmania infantum


TKIT12059 TKIT12059M DNA, Microsporum canis


TKIT12062 TKIT12062M DNA, Mycoplasma felis

TKIT12065 TKIT12065M DNA, Mycoplasma haemofelis

TKIT12066 TKIT12066M DNA, Mycoplasma species haemofelis &

haemocanis

TKIT12068 TKIT12068M DNA, Neospora caninum









PorcineTKIT11004 TKIT11004M DNA, African Trypanosomiasis




TKIT12092 TKIT12092M DNA, Mycoplasma hyopneumoniae

TKIT12066 TKIT12066M DNA, Mycoplasma species haemofelis & haemocanis

TKIT12093 TKIT12093M DNA, Mycoplasma suis

TKIT12068 TKIT12068M DNA, Neospora caninum

TKIT12088 TKIT12088M DNA, Porcine circovirus 1

TKIT12072 TKIT12072M DNA, Porcine circovirus 2

TKIT12074 TKIT12074M RNA, Porcine Reproductive and Respiratory

Syndrome virus













PisceanTKIT11006 TKIT11006M DNA, Aeromonas hydrophila

TKIT12031 TKIT12031M DNA, Cyprinid herpes virus 3


TKIT12048 TKIT12048M RNA, Grass Carp Reovirus

TKIT12056 TKIT12056M RNA, Infectious Hematopoietic Necrosis virus

A small list of detection kits aimed

at detecting some particularly

harmful viruses and bacteria such

as Rift Valley Virus and E.coli 0157.

If your test is not available contact

PCRmax or your local dealer to

enquire about getting a custom kit

made for you.

Biothreat BiothreatTKIT03001 TKIT03001M DNA, Bacillus anthracis

TKIT11001 TKIT11001M DNA, Burkholderia mallei








TKIT10044 TKIT10044M RNA, H1N1 influenza



TKIT12096 TKIT12096M DNA, Vaccinia virus


TKIT12057 TKIT12057M RNA, Infectious Pancreatic Necrosis virus

TKIT10111 TKIT10111M DNA, Mycobacterium marinum and

ulcerans

TKIT09001 TKIT09001M DNA, Shewanella putrefaciens

TKIT12077 TKIT12077M RNA, Spring Viremia of Carp virus

TKIT12087 TKIT12087M RNA, Viral Hemorrhagic Septicemia virus

OthersTKIT12002 TKIT12002M DNA, Aleutian Disease virus

TKIT12012 TKIT12012M DNA, Batrachochytrium dendrobatidis

TKIT12010 TKIT12010M DNA, Botrytis cinerea

TKIT12089 TKIT12089M DNA, Camelpox virus

TKIT12027 TKIT12027M DNA, Clavibacter michiganensis subspecies

michiganensis

TKIT11015 TKIT11015M DNA, Cryptococcus neoformans


TKIT11020 TKIT11020M RNA, Dobrava-Belgrade virus

TKIT12034 TKIT12034M RNA, Epizootic Hemorrhagic Disease virus


TKIT12053 TKIT12053M RNA, Israeli Acute Paralysis virus

TKIT12061 TKIT12061M RNA, Maize Dwarf Mosaic virus

TKIT03005 TKIT03005M RNA, Sudan Ebola virus

TKIT12075 TKIT12075M RNA, Sugarcane Mosaic virus

TKIT03006 TKIT03006M RNA, Taï Forest Ebola virus

TKIT12086 TKIT12086M RNA, Vesivirus2117

TKIT04002 TKIT04002M RNA, Slow Bee Paralysis virus

TKIT03002 TKIT03002M RNA, Zaire ebola virus (with Mastermix)


Meat Speciation kitsTKIT06024 TKIT06024M DNA, Bos taurus (bovine/beef)

TKIT06022 TKIT06022M DNA, Bubalus bubalis (buffalo)

TKIT06032 TKIT06032M DNA, Felis catus (cat)

TKIT06033 TKIT06033M DNA, Gallus gallus (chicken)

TKIT06026 TKIT06026M DNA, Capreolus capreolus (deer)

TKIT06027 TKIT06027M DNA, Canis familiaris (dog)

TKIT06030 TKIT06030M DNA, Equus asinus (donkey)

TKIT06021 TKIT06021M DNA, Anas platyrhynchos (duck)

TKIT06028 TKIT06028M DNA, Capra hircus (goat)

TKIT06037 TKIT06037M DNA, Melanogrammus aeglefinus (haddock)

TKIT06031 TKIT06031M DNA, Equus caballus (horse)

TKIT06049 TKIT06049M DNA, Struthio camelus (ostrich)

TKIT06050 TKIT06050M DNA, Sus scrofa (pig/pork)

TKIT06042 TKIT06042M DNA, Ovis aries (sheep)

TKIT06038 TKIT06038M DNA, Meleagris gallopavo (turkey)

TKIT06043 TKIT06043M DNA, Phacochoerus africanus (warthog)

TKIT06052 TKIT06052M DNA, Universal meat detection

Fish Speciation kitsTKIT06045 TKIT06045M DNA, Pleuronectes platessa (european plaice)

TKIT06037 TKIT06037M DNA, Melanogrammus aeglefinus (haddock)

TKIT06022 TKIT06022M DNA, Bubalus bubalis (buffalo)

TKIT06035 TKIT06035M DNA, Gadus morhua (cod)

TKIT06040 TKIT06040M DNA, Merlangius merlangus (whiting)

TKIT06048 TKIT06048M DNA, Pollachius virens (pollock)

TKIT06051 TKIT06051M DNA, Universal fish detection

AllergensTKIT06039 TKIT06039M DNA, Macadamia integrifolia (Macadamia)

TKIT06020 TKIT06020M DNA, Anacardium occidentale (cashew)

TKIT06047 TKIT06047M DNA, Pistacia vera (pistacio)

TKIT06025 TKIT06025M DNA, Corylus avellana (hazelnut)

TKIT06044 TKIT06044M DNA, Prunus dulcis (almond)

TKIT06036 TKIT06036M DNA, Juglans regia (walnut)

TKIT06046 TKIT06046M DNA, Pisum Satvium (green pea)

TKIT06018 TKIT06018M DNA, Apium graveolens var. dulce (Celery)

Pathogen contaminationTKIT07002 TKIT07002M DNA, Bacillus cereus E33

TKIT07003 TKIT07003M DNA, Brucella genus (All species)




TKIT06002 TKIT06002M DNA, Clostridium estertheticum

TKIT07005 TKIT07005M DNA, Clostridium perfringens Types A&B





TKIT06003 TKIT06003M DNA, Dekkera bruxellensis

TKIT07006 TKIT07006M DNA, Enterococcus faecalis

TKIT07007 TKIT07007M QPCR Kit, DNA, Enterococcus faecium



TKIT06004 TKIT06004M DNA, Eubacteria Bacteria Domain



TKIT07008 TKIT07008M RNA, Hepatitis A virus

TKIT07009 TKIT07009M RNA, Hepatitis E virus

TKIT07010 TKIT07010M DNA, JC virus

TKIT10102 TKIT10102M DNA, Legionella pneumophila




paratuberculosis

TKIT07014 TKIT07014M DNA, All Naegleria species

TKIT07015 TKIT07015M RNA, Norovirus genotypes 1 and 2

TKIT07016 TKIT07016M DNA, Pseudomonas aeruginosa



TKIT09001 TKIT09001M DNA, Shewanella putrefaciens



TKIT07018 TKIT07018M DNA, Shigella (All species)

TKIT07017 TKIT07017M DNA, Simkania negevensis

TKIT02001 TKIT02001M DNA, Staphylococcus aureus



TKIT07019 TKIT07019M DNA, All Vibrio cholerae subspecies

TKIT07020 TKIT07020M DNA, All Vibrio species

TKIT02003 TKIT02003M DNA, Yersinia enterocolitica

OthersTKIT07001 TKIT07001M DNA, Bifidobacterium bifidum

TKIT06001 TKIT06001M DNA, Bifidobacterium longum

TKIT06015 TKIT06015M DNA, Lactobacillus plantarum

qPCR is the fastest and most accurate way to screen water and

food for pathogens. Prime Pro qPCR reagents also facilitate

speciation and allergen detection.

• Speciation of meat and fish

• Pathogen contamination

• Allergen detection

• GMO detection/quantification

Labelling of genetically modified organisms is now mandatory

in many regions, including the European Union. To guarantee

consumer choice between GM and non-GM products,

screening of samples is performed by PCR amplification of

regulatory sequences frequently introduced into genetically

modified organisms. Cauliflower mosaic virus is commonly

used as a means of GM detection

Food and water

48 wells. Less DNA. Due to lack of dependence on large sample volumes, the Techne Prime Pro 48 is validated for

between 5-20ul meaning you can do more tests with your precious DNA sample and cut reagent

costs in half.

48 wells. Less Waste. Due to patented thermal technology and 5X higher uniformity than a typical 96 well system, there

is no dependency on unnecessary replicates to mitigate poor system performance. This means less

sample waste and more valid experiments.

48 wells. Less time. A single peltier and low block mass enables fast ramp rates. Comfortably perform 40 cycles in 40

minutes. Zero settle time is required to hit precise temperatures allowing optimised 40 cycle runs

in 20 minutes. Process more samples per day than a typical 96 well qPCR system which can take

80 minutes per run.

Do more with less with Techne Prime Pro 48

• Higher uniformity than a 96 well system

• Higher quality data than a 96 well system

• Higher throughput than a 96 well system

• Faster ramp rate than a 96 well system

• Shorter run times than a 96 well system

• Use less DNA than a 96 well system

• Needs fewer replicates than a 96 well system

• Lower running costs than a 96 well system

• Lower price than a 96 well system

Get more for less...

...with a 48 well block

Bibby Scientific - UK (Group HQ)

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United Kingdom

T: +44 (0)1785 812121

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E: [email protected]

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T: +1 609 589 2560

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T: +357 22 660 423

F: +357 22 660 424


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Singapore 049712

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F: +44 (0) 1785 810405


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Prime Pro qPCR reagent kits - Techne · 2020. 9. 23. · TKIT08001 TKIT08001M DNA, Brucella abortus...

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Transcript of Prime Pro qPCR reagent kits - Techne · 2020. 9. 23. · TKIT08001 TKIT08001M DNA, Brucella abortus...